(Circular) (Six-base) MAPSORT of: pFN21AE0779.seq Check: 5513 from: 1 to: 5749 pFN21AE0779 5749 bp With 182 enzymes: SgfI * April 15, 2010 09:34 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3559 278 Size: 53 83 186 936 2023 2468 Fragments arranged by size: 2468 2023 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3092 1937 Size: 1155 4594 Acc65I G'GTAC_C Cuts at: 3070 3070 Size: 5749 AclI AA'CG_TT Cuts at: 1563 3876 4249 1563 Size: 2313 373 3063 Fragments arranged by size: 3063 2313 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2294 2576 3812 5196 5464 1632 Size: 662 282 1236 1384 268 1917 Fragments arranged by size: 1917 1384 1236 662 282 268 AfeI AGC'GCT Cuts at: 2953 2953 Size: 5749 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5552 AflIII A'CryG_T Cuts at: 1203 4648 5625 1203 Size: 3445 977 1327 Fragments arranged by size: 3445 1327 977 AgeI A'CCGG_T Cuts at: 4580 4580 Size: 5749 AhdI GACnn_n'nnGTC Cuts at: 4478 4478 Size: 5749 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5749 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5749 AlwNI CAG_nnn'CTG Cuts at: 2161 3492 5064 2161 Size: 1331 1572 2846 Fragments arranged by size: 2846 1572 1331 ApaLI G'TGCA_C Cuts at: 2483 3806 4962 2483 Size: 1323 1156 3270 Fragments arranged by size: 3270 1323 1156 ApoI r'AATT_y Cuts at: 1493 3058 3284 3436 5545 1493 Size: 1565 226 152 2109 1697 Fragments arranged by size: 2109 1697 1565 226 152 AseI AT'TA_AT Cuts at: 160 1032 4303 160 Size: 872 3271 1606 Fragments arranged by size: 3271 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5749 AvaI C'yCGr_G Cuts at: 1870 1943 2681 2708 2862 3074 1870 Size: 73 738 27 154 212 4545 Fragments arranged by size: 4545 738 212 154 73 27 BamHI G'GATC_C Cuts at: 3079 3446 3079 Size: 367 5382 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2982 3070 4525 618 Size: 325 217 627 1195 88 1455 1842 Fragments arranged by size: 1842 1455 1195 627 325 217 88 BanII G_rGCy'C Cuts at: 1391 2100 2187 2337 2421 2688 3068 1391 Size: 709 87 150 84 267 380 4072 Fragments arranged by size: 4072 709 380 267 150 87 84 BbeI G_GCGC'C Cuts at: 2986 2986 Size: 5749 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4778 BbvCI CC'TCA_GC Cuts at: 2874 2874 Size: 5749 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2031 2065 2365 2399 3940 Size: 34 50 34 10 34 300 34 1541 Cuts at: 3940 3974 1903 Size: 34 3678 Fragments arranged by size: 3678 1541 300 50 34 34 34 34 34 10 BciVI GTATCCnnnnn_n' Cuts at: 3641 4851 3641 Size: 1210 4539 BclI T'GATC_A Cuts at: 1548 1548 Size: 5749 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2504 2900 4360 Size: 107 122 71 631 605 832 396 1460 Cuts at: 4360 136 Size: 1525 Fragments arranged by size: 1525 1460 832 631 605 396 122 107 71 BglII A'GATC_T Cuts at: 5744 5744 Size: 5749 BlpI GC'TnA_GC Cuts at: 3166 3166 Size: 5749 Bme1580I G_kGCm'C Cuts at: 1790 2487 3810 4966 1790 Size: 697 1323 1156 2573 Fragments arranged by size: 2573 1323 1156 697 BmgBI CAC'GTC Cuts at: 1318 2298 1318 Size: 980 4769 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3480 4438 442 Size: 416 53 83 399 24 2063 958 1753 Fragments arranged by size: 2063 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5749 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2084 2150 2537 3010 4409 1239 Size: 845 66 387 473 1399 2579 Fragments arranged by size: 2579 1399 845 473 387 66 Bpu10I CC'TnA_GC Cuts at: 2874 2874 Size: 5749 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3874 4739 5037 3874 Size: 865 298 4586 Fragments arranged by size: 4586 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4412 882 Size: 617 2913 2219 Fragments arranged by size: 2913 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3540 493 Size: 711 2336 2702 Fragments arranged by size: 2702 2336 711 BsaBI GATnn'nnATC Cuts at: 2963 5743 2963 Size: 2780 2969 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2739 2754 2849 2983 Size: 53 83 186 936 1206 15 95 134 Cuts at: 2983 3556 3938 4573 275 Size: 573 382 635 1451 Fragments arranged by size: 1451 1206 936 635 573 382 186 134 95 83 53 15 BsaWI w'CCGG_w Cuts at: 4182 4580 4854 5001 5412 4182 Size: 398 274 147 411 4519 Fragments arranged by size: 4519 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5524 Fragments arranged by size: 5524 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2078 2081 2108 2762 2962 3050 1186 Size: 892 3 27 654 200 88 3885 Fragments arranged by size: 3885 892 654 200 88 27 3 BseYI C'CCAG_C Cuts at: 2341 2499 2749 3041 4952 5448 2341 Size: 158 250 292 1911 496 2642 Fragments arranged by size: 2642 1911 496 292 250 158 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5176 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2904 3199 3960 4988 1540 Size: 275 186 903 295 761 1028 2301 Fragments arranged by size: 2301 1028 903 761 295 275 186 BsiHKAI G_wGCw'C Cuts at: 2100 2155 2187 2487 2688 2976 3810 3895 4966 Size: 55 32 300 201 288 834 85 1071 Cuts at: 4966 2100 Size: 2883 Fragments arranged by size: 2883 1071 834 300 288 201 85 55 32 BsmI GAATG_Cn' Cuts at: 3260 3353 3260 Size: 93 5656 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2100 2155 2187 2337 2421 2487 2688 Size: 399 310 55 32 150 84 66 201 Cuts at: 2688 2976 3068 3810 3895 4966 1391 Size: 288 92 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 310 288 201 150 92 85 84 66 55 32 BspEI T'CCGG_A Cuts at: 5412 5412 Size: 5749 BspHI T'CATG_A Cuts at: 3638 5368 3638 Size: 1730 4019 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3037 3104 844 Size: 2193 67 3489 Fragments arranged by size: 3489 2193 67 BsrBI CCG'CTC Cuts at: 1394 1823 2704 3026 3636 1394 Size: 429 881 322 610 3507 Fragments arranged by size: 3507 881 610 429 322 BsrDI GCAATG_nn' Cuts at: 65 1248 4244 4418 65 Size: 1183 2996 174 1396 Fragments arranged by size: 2996 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2032 2592 2796 4393 4580 1700 Size: 110 222 560 204 1597 187 2869 Fragments arranged by size: 2869 1597 560 222 204 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5749 BssHII G'CGCG_C Cuts at: 1925 2124 2346 2691 1925 Size: 199 222 345 4983 Fragments arranged by size: 4983 345 222 199 BssSI C'ACGA_G Cuts at: 2623 3809 4821 2623 Size: 1186 1012 3551 Fragments arranged by size: 3551 1186 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5749 BstBI TT'CG_AA Cuts at: 4553 4553 Size: 5749 BstEII G'GTnAC_C Cuts at: 1184 2239 1184 Size: 1055 4694 BstYI r'GATC_y Cuts at: 1972 3079 3446 3831 3848 5300 5386 5398 5744 Size: 1107 367 385 17 1452 86 12 346 Cuts at: 5744 1972 Size: 1977 Fragments arranged by size: 1977 1452 1107 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2305 2478 2533 2898 513 Size: 552 1240 173 55 365 3364 Fragments arranged by size: 3364 1240 552 365 173 55 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5010 Fragments arranged by size: 5010 683 56 BtsI GCAGTG_nn' Cuts at: 3024 3265 4059 4079 3024 Size: 241 794 20 4694 Fragments arranged by size: 4694 794 241 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5749 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5714 DraI TTT'AAA Cuts at: 3053 3053 Size: 5749 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4756 809 Size: 507 3440 1802 Fragments arranged by size: 3440 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2224 2617 2652 Size: 54 1425 50 275 18 394 393 35 Cuts at: 2652 2805 2901 3196 4085 5714 8 Size: 153 96 295 889 1629 43 Fragments arranged by size: 1629 1425 889 394 393 295 275 153 96 54 50 43 35 18 EagI C'GGCC_G Cuts at: 1537 1812 2901 3196 1537 Size: 275 1089 295 4090 Fragments arranged by size: 4090 1089 295 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2768 3027 3679 1353 Size: 1415 259 652 3423 Fragments arranged by size: 3423 1415 652 259 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2051 2500 3026 4336 4710 4856 Size: 1064 138 462 449 526 1310 374 146 Cuts at: 4856 387 Size: 1280 Fragments arranged by size: 1310 1280 1064 526 462 449 374 146 138 EcoICRI GAG'CTC Cuts at: 2098 2185 2686 2098 Size: 87 501 5161 Fragments arranged by size: 5161 501 87 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2084 2150 2294 2537 2576 3010 3812 Size: 393 452 66 144 243 39 434 802 Cuts at: 3812 4409 5196 5464 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 802 787 597 452 434 393 268 243 144 66 39 EcoNI CCTnn'n_nnAGG Cuts at: 2210 2807 2210 Size: 597 5152 EcoO109I rG'GnC_Cy Cuts at: 2434 2551 2713 2434 Size: 117 162 5470 Fragments arranged by size: 5470 162 117 EcoRI G'AATT_C Cuts at: 3058 3436 3058 Size: 378 5371 FspI TGC'GCA Cuts at: 4255 4255 Size: 5749 HaeII r_GCGC'y Cuts at: 1678 2599 2750 2955 2986 4896 1678 Size: 921 151 205 31 1910 2531 Fragments arranged by size: 2531 1910 921 205 151 31 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4390 4422 4464 4496 1373 Size: 32 135 32 2818 32 42 32 2626 Fragments arranged by size: 2818 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3093 3339 669 Size: 988 281 1155 246 3079 Fragments arranged by size: 3079 1155 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5749 HpaI GTT'AAC Cuts at: 3339 3339 Size: 5749 KasI G'GCGC_C Cuts at: 2982 2982 Size: 5749 KpnI G_GTAC'C Cuts at: 3074 3074 Size: 5749 MfeI C'AATT_G Cuts at: 3348 3348 Size: 5749 MluI A'CGCG_T Cuts at: 5625 5625 Size: 5749 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4863 5047 1305 Size: 3558 184 2007 Fragments arranged by size: 3558 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3927 Fragments arranged by size: 3927 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3707 4066 4225 518 Size: 648 2541 359 159 2042 Fragments arranged by size: 2541 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2480 2490 2503 2535 2900 2998 3165 3842 Size: 874 10 13 32 365 98 167 677 Cuts at: 3842 4990 5235 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 874 677 365 245 167 98 32 13 10 NaeI GCC'GGC Cuts at: 2034 2594 2034 Size: 560 5189 NarI GG'CG_CC Cuts at: 2983 2983 Size: 5749 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5197 NdeI CA'TA_TG Cuts at: 387 387 Size: 5749 NgoMIV G'CCGG_C Cuts at: 2032 2592 2032 Size: 560 5189 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5749 NotI GC'GGCC_GC Cuts at: 3196 3196 Size: 5749 NspI r_CATG'y Cuts at: 1132 1229 3107 4652 1132 Size: 97 1878 1545 2229 Fragments arranged by size: 2229 1878 1545 97 PasI CC'CwG_GG Cuts at: 1348 2206 2790 1348 Size: 858 584 4307 Fragments arranged by size: 4307 858 584 PciI A'CATG_T Cuts at: 4648 4648 Size: 5749 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5749 PmeI GTTT'AAAC Cuts at: 3053 3053 Size: 5749 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3818 3850 5331 5363 3818 Size: 32 1481 32 4204 Fragments arranged by size: 4204 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2434 2551 2434 Size: 117 5632 PshAI GACnn'nnGTC Cuts at: 1613 2162 1613 Size: 549 5200 PsiI TTA'TAA Cuts at: 3319 3319 Size: 5749 PstI C_TGCA'G Cuts at: 830 3101 830 Size: 2271 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5749 PvuII CAG'CTG Cuts at: 2503 2503 Size: 5749 SacI G_AGCT'C Cuts at: 2100 2187 2688 2100 Size: 87 501 5161 Fragments arranged by size: 5161 501 87 SacII CC_GC'GG Cuts at: 2481 2536 2901 2481 Size: 55 365 5329 Fragments arranged by size: 5329 365 55 SalI G'TCGA_C Cuts at: 1936 3091 1936 Size: 1155 4594 SapI GCTCTTCn'nnn_ Cuts at: 2768 2768 Size: 5749 SbfI CC_TGCA'GG Cuts at: 3101 3101 Size: 5749 ScaI AGT'ACT Cuts at: 3997 5709 3997 Size: 1712 4037 SfcI C'TryA_G Cuts at: 826 1045 2401 3097 4232 4913 5104 826 Size: 219 1356 696 1135 681 191 1471 Fragments arranged by size: 1471 1356 1135 696 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2900 2900 Size: 5749 SfoI GGC'GCC Cuts at: 2984 2984 Size: 5749 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5749 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5749 SmaI CCC'GGG Cuts at: 1872 2864 3076 1872 Size: 992 212 4545 Fragments arranged by size: 4545 992 212 SmlI C'TyrA_G Cuts at: 820 1017 1943 3853 4754 5016 820 Size: 197 926 1910 901 262 1553 Fragments arranged by size: 1910 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3540 493 Size: 3047 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5749 SphI G_CATG'C Cuts at: 1132 3107 1132 Size: 1975 3774 SspI AAT'ATT Cuts at: 5 52 3673 5 Size: 47 3621 2081 Fragments arranged by size: 3621 2081 47 StuI AGG'CCT Cuts at: 1517 2629 1517 Size: 1112 4637 StyI C'CwwG_G Cuts at: 513 1065 2457 3188 513 Size: 552 1392 731 3074 Fragments arranged by size: 3074 1392 731 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2258 2624 3776 3793 3946 4131 4576 Size: 1255 678 366 1152 17 153 185 445 Cuts at: 4576 325 Size: 1498 Fragments arranged by size: 1498 1255 1152 678 445 366 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2520 3995 5707 Size: 274 80 33 51 1443 543 1475 1712 Cuts at: 5707 96 Size: 138 Fragments arranged by size: 1712 1475 1443 543 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5709 5741 5709 Size: 32 5717 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5749 XbaI T'CTAG_A Cuts at: 3085 3085 Size: 5749 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2219 2219 Size: 5749 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5749 XmaI C'CCGG_G Cuts at: 1870 2862 3074 1870 Size: 992 212 4545 Fragments arranged by size: 4545 992 212 XmnI GAAnn'nnTTC Cuts at: 3878 3878 Size: 5749 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3557 276 Size: 53 83 186 936 2023 2468 Fragments arranged by size: 2468 2023 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SanDI SexAI SrfI SwaI