(Circular) (Six-base) MAPSORT of: pFN21AE0989.seq Check: 3818 from: 1 to: 6574 pFN21AE0989 6574 bp With 182 enzymes: SgfI * April 15, 2010 09:34 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4384 278 Size: 53 83 186 936 2848 2468 Fragments arranged by size: 2848 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3917 1937 Size: 1980 4594 Acc65I G'GTAC_C Cuts at: 3895 3895 Size: 6574 AclI AA'CG_TT Cuts at: 1563 4701 5074 1563 Size: 3138 373 3063 Fragments arranged by size: 3138 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2186 2511 2529 3702 4637 6021 6289 1632 Size: 554 325 18 1173 935 1384 268 1917 Fragments arranged by size: 1917 1384 1173 935 554 325 268 18 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6377 AflIII A'CryG_T Cuts at: 1203 5473 6450 1203 Size: 4270 977 1327 Fragments arranged by size: 4270 1327 977 AgeI A'CCGG_T Cuts at: 5405 5405 Size: 6574 AhdI GACnn_n'nnGTC Cuts at: 5303 5303 Size: 6574 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6574 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6574 AlwNI CAG_nnn'CTG Cuts at: 3034 3061 4317 5889 3034 Size: 27 1256 1572 3719 Fragments arranged by size: 3719 1572 1256 27 ApaLI G'TGCA_C Cuts at: 3810 3830 4631 5787 3810 Size: 20 801 1156 4597 Fragments arranged by size: 4597 1156 801 20 ApoI r'AATT_y Cuts at: 1493 2637 3883 4109 4261 6370 1493 Size: 1144 1246 226 152 2109 1697 Fragments arranged by size: 2109 1697 1246 1144 226 152 AseI AT'TA_AT Cuts at: 160 1032 2270 5128 160 Size: 872 1238 2858 1606 Fragments arranged by size: 2858 1606 1238 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6574 AvaI C'yCGr_G Cuts at: 1870 1943 3121 3899 1870 Size: 73 1178 778 4545 Fragments arranged by size: 4545 1178 778 73 BamHI G'GATC_C Cuts at: 2806 3687 3904 4271 2806 Size: 881 217 367 5109 Fragments arranged by size: 5109 881 367 217 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3895 5350 618 Size: 325 217 627 2108 1455 1842 Fragments arranged by size: 2108 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3893 1391 Size: 2502 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5603 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4765 4799 1903 Size: 34 2828 34 3678 Fragments arranged by size: 3678 2828 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2678 4466 5676 2678 Size: 1788 1210 3576 Fragments arranged by size: 3576 1788 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6574 BfrBI ATG'CAT Cuts at: 2139 2139 Size: 6574 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5185 136 Size: 107 122 71 631 605 3513 1525 Fragments arranged by size: 3513 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2522 6569 2522 Size: 4047 2527 BlpI GC'TnA_GC Cuts at: 3991 3991 Size: 6574 Bme1580I G_kGCm'C Cuts at: 1790 2506 3814 3834 4635 5791 1790 Size: 716 1308 20 801 1156 2573 Fragments arranged by size: 2573 1308 1156 801 716 20 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6574 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2716 3566 3856 Size: 416 53 83 399 24 1299 850 290 Cuts at: 3856 4305 5263 442 Size: 449 958 1753 Fragments arranged by size: 1753 1299 958 850 449 416 399 290 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6574 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3234 5234 1239 Size: 1995 2000 2579 Fragments arranged by size: 2579 2000 1995 Bpu10I CC'TnA_GC Cuts at: 2967 3062 2967 Size: 95 6479 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2167 4699 5564 5862 2167 Size: 2532 865 298 2879 Fragments arranged by size: 2879 2532 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5237 882 Size: 617 3738 2219 Fragments arranged by size: 3738 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2046 4365 493 Size: 711 842 2319 2702 Fragments arranged by size: 2702 2319 842 711 BsaBI GATnn'nnATC Cuts at: 6568 6568 Size: 6574 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4381 4763 5398 275 Size: 53 83 186 936 2848 382 635 1451 Fragments arranged by size: 2848 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5007 5405 5679 5826 6237 5007 Size: 398 274 147 411 5344 Fragments arranged by size: 5344 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6349 Fragments arranged by size: 6349 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6574 BseYI C'CCAG_C Cuts at: 3009 3222 5777 6273 3009 Size: 213 2555 496 3310 Fragments arranged by size: 3310 2555 496 213 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3689 1163 Size: 573 1953 4048 Fragments arranged by size: 4048 1953 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2822 4024 4785 5813 1540 Size: 275 186 821 1202 761 1028 2301 Fragments arranged by size: 2301 1202 1028 821 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3814 3834 4635 4720 5791 3814 Size: 20 801 85 1071 4597 Fragments arranged by size: 4597 1071 801 85 20 BsmI GAATG_Cn' Cuts at: 2141 4085 4178 2141 Size: 1944 93 4537 Fragments arranged by size: 4537 1944 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2506 3814 3834 3893 4635 4720 5791 Size: 399 716 1308 20 59 742 85 1071 Cuts at: 5791 1391 Size: 2174 Fragments arranged by size: 2174 1308 1071 742 716 399 85 59 20 BspEI T'CCGG_A Cuts at: 6237 6237 Size: 6574 BspHI T'CATG_A Cuts at: 2784 4463 6193 2784 Size: 1679 1730 3165 Fragments arranged by size: 3165 1730 1679 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3388 3929 844 Size: 2544 541 3489 Fragments arranged by size: 3489 2544 541 BsrBI CCG'CTC Cuts at: 1394 1823 4461 1394 Size: 429 2638 3507 Fragments arranged by size: 3507 2638 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3601 5069 5243 65 Size: 1183 2353 1468 174 1396 Fragments arranged by size: 2353 1468 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5218 5405 1700 Size: 110 3408 187 2869 Fragments arranged by size: 3408 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6574 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6574 BssSI C'ACGA_G Cuts at: 4634 5646 4634 Size: 1012 5562 BstAPI GCAn_nnn'nTGC Cuts at: 794 3034 3578 794 Size: 2240 544 3790 Fragments arranged by size: 3790 2240 544 BstBI TT'CG_AA Cuts at: 5378 5378 Size: 6574 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6574 BstXI CCAn_nnnn'nTGG Cuts at: 2360 3556 2360 Size: 1196 5378 BstYI r'GATC_y Cuts at: 1972 2522 2806 3687 3904 4271 4656 4673 6125 Size: 550 284 881 217 367 385 17 1452 Cuts at: 6125 6211 6223 6569 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 881 550 385 367 346 284 217 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3340 3340 Size: 6574 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 6022 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5835 Fragments arranged by size: 5835 683 56 BtsI GCAGTG_nn' Cuts at: 3032 4090 4884 4904 3032 Size: 1058 794 20 4702 Fragments arranged by size: 4702 1058 794 20 ClaI AT'CG_AT Cuts at: 1554 2988 1554 Size: 1434 5140 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6539 DraI TTT'AAA Cuts at: 2635 2913 3289 3878 2635 Size: 278 376 589 5331 Fragments arranged by size: 5331 589 376 278 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5581 809 Size: 507 4265 1802 Fragments arranged by size: 4265 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2094 2675 3679 Size: 54 1425 50 275 18 264 581 1004 Cuts at: 3679 3852 4021 4910 6539 8 Size: 173 169 889 1629 43 Fragments arranged by size: 1629 1425 1004 889 581 275 264 173 169 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4021 1537 Size: 275 2209 4090 Fragments arranged by size: 4090 2209 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2105 3222 4504 1353 Size: 752 1117 1282 3423 Fragments arranged by size: 3423 1282 1117 752 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5161 5535 5681 387 Size: 1064 138 3572 374 146 1280 Fragments arranged by size: 3572 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2186 2511 2529 3234 3702 4637 5234 Size: 393 554 325 18 705 468 935 597 Cuts at: 5234 6021 6289 1239 Size: 787 268 1524 Fragments arranged by size: 1524 935 787 705 597 554 468 393 325 268 18 EcoO109I rG'GnC_Cy Cuts at: 2876 3560 2876 Size: 684 5890 EcoRI G'AATT_C Cuts at: 3883 4261 3883 Size: 378 6196 EcoRV GAT'ATC Cuts at: 3461 3461 Size: 6574 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3508 3508 Size: 6574 FspI TGC'GCA Cuts at: 5080 5080 Size: 6574 HaeII r_GCGC'y Cuts at: 1678 5721 1678 Size: 4043 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2468 2500 5215 5247 5289 Size: 32 135 32 896 32 2715 32 42 Cuts at: 5289 5321 1373 Size: 32 2626 Fragments arranged by size: 2715 2626 896 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3417 3918 4164 669 Size: 988 281 1479 501 246 3079 Fragments arranged by size: 3079 1479 988 501 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6574 HpaI GTT'AAC Cuts at: 4164 4164 Size: 6574 KpnI G_GTAC'C Cuts at: 3899 3899 Size: 6574 MfeI C'AATT_G Cuts at: 4173 4173 Size: 6574 MluI A'CGCG_T Cuts at: 6450 6450 Size: 6574 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2542 2980 3213 3372 3449 5688 5872 1305 Size: 1237 438 233 159 77 2239 184 2007 Fragments arranged by size: 2239 2007 1237 438 233 184 159 77 MscI TGG'CCA Cuts at: 10 64 1489 1832 2096 2677 3681 3854 10 Size: 54 1425 343 264 581 1004 173 2730 Fragments arranged by size: 2730 1425 1004 581 343 264 173 54 MslI CAynn'nnrTG Cuts at: 518 1166 2021 2184 2358 2549 3174 4532 4891 Size: 648 855 163 174 191 625 1358 359 Cuts at: 4891 5050 518 Size: 159 2042 Fragments arranged by size: 2042 1358 855 648 625 359 191 174 163 159 MspA1I CmG'CkG Cuts at: 1606 2692 2923 3677 3990 4667 5815 6060 1606 Size: 1086 231 754 313 677 1148 245 2120 Fragments arranged by size: 2120 1148 1086 754 677 313 245 231 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6022 NdeI CA'TA_TG Cuts at: 387 387 Size: 6574 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6574 NotI GC'GGCC_GC Cuts at: 4021 4021 Size: 6574 NsiI A_TGCA'T Cuts at: 2141 2141 Size: 6574 NspI r_CATG'y Cuts at: 1132 1229 3142 3932 5477 1132 Size: 97 1913 790 1545 2229 Fragments arranged by size: 2229 1913 1545 790 97 PasI CC'CwG_GG Cuts at: 1348 2606 1348 Size: 1258 5316 PciI A'CATG_T Cuts at: 5473 5473 Size: 6574 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6574 PmeI GTTT'AAAC Cuts at: 3878 3878 Size: 6574 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4643 4675 6156 6188 4643 Size: 32 1481 32 5029 Fragments arranged by size: 5029 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3560 3560 Size: 6574 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6574 PsiI TTA'TAA Cuts at: 3774 4144 3774 Size: 370 6204 PstI C_TGCA'G Cuts at: 830 3039 3926 830 Size: 2209 887 3478 Fragments arranged by size: 3478 2209 887 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6574 PvuII CAG'CTG Cuts at: 2692 2923 3677 2692 Size: 231 754 5589 Fragments arranged by size: 5589 754 231 SalI G'TCGA_C Cuts at: 1936 3916 1936 Size: 1980 4594 SanDI GG'GwC_CC Cuts at: 3560 3560 Size: 6574 SbfI CC_TGCA'GG Cuts at: 3926 3926 Size: 6574 ScaI AGT'ACT Cuts at: 3440 4822 6534 3440 Size: 1382 1712 3480 Fragments arranged by size: 3480 1712 1382 SfcI C'TryA_G Cuts at: 826 1045 3035 3780 3922 5057 5738 5929 826 Size: 219 1990 745 142 1135 681 191 1471 Fragments arranged by size: 1990 1471 1135 745 681 219 191 142 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6574 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6574 SmaI CCC'GGG Cuts at: 1872 3901 1872 Size: 2029 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2146 4678 5579 5841 820 Size: 197 926 203 2532 901 262 1553 Fragments arranged by size: 2532 1553 926 901 262 203 197 SnaBI TAC'GTA Cuts at: 493 4365 493 Size: 3872 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6574 SphI G_CATG'C Cuts at: 1132 3932 1132 Size: 2800 3774 SspI AAT'ATT Cuts at: 5 52 2317 2331 3799 4498 5 Size: 47 2265 14 1468 699 2081 Fragments arranged by size: 2265 2081 1468 699 47 14 StuI AGG'CCT Cuts at: 1517 1517 Size: 6574 StyI C'CwwG_G Cuts at: 513 1065 4013 513 Size: 552 2948 3074 Fragments arranged by size: 3074 2948 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2804 2836 2862 3269 3838 4601 4618 Size: 1255 1224 32 26 407 569 763 17 Cuts at: 4618 4771 4956 5401 325 Size: 153 185 445 1498 Fragments arranged by size: 1498 1255 1224 763 569 445 407 185 153 32 26 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3438 4820 6532 Size: 274 80 33 51 1443 1461 1382 1712 Cuts at: 6532 96 Size: 138 Fragments arranged by size: 1712 1461 1443 1382 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6534 6566 6534 Size: 32 6542 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6574 XbaI T'CTAG_A Cuts at: 3910 3910 Size: 6574 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2029 2029 Size: 6574 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6574 XmaI C'CCGG_G Cuts at: 1870 3899 1870 Size: 2029 4545 XmnI GAAnn'nnTTC Cuts at: 2775 4703 2775 Size: 1928 4646 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4382 276 Size: 53 83 186 936 2848 2468 Fragments arranged by size: 2848 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI