(Circular) (Six-base) MAPSORT of: pFN21AB0409.seq Check: 2549 from: 1 to: 6103 pFN21AB0409 6103 bp With 182 enzymes: SgfI * April 15, 2010 09:34 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3913 278 Size: 53 83 186 936 2377 2468 Fragments arranged by size: 2468 2377 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3446 1937 Size: 1509 4594 Acc65I G'GTAC_C Cuts at: 3252 3424 3252 Size: 172 5931 AclI AA'CG_TT Cuts at: 1563 4230 4603 1563 Size: 2667 373 3063 Fragments arranged by size: 3063 2667 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2033 4166 5550 5818 1632 Size: 401 2133 1384 268 1917 Fragments arranged by size: 2133 1917 1384 401 268 AfeI AGC'GCT Cuts at: 2513 2513 Size: 6103 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5906 AflIII A'CryG_T Cuts at: 1203 2190 3162 5002 5979 1203 Size: 987 972 1840 977 1327 Fragments arranged by size: 1840 1327 987 977 972 AgeI A'CCGG_T Cuts at: 4934 4934 Size: 6103 AhdI GACnn_n'nnGTC Cuts at: 2557 4832 2557 Size: 2275 3828 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6103 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6103 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3149 3181 3149 Size: 32 6071 AlwNI CAG_nnn'CTG Cuts at: 2689 3033 3046 3846 5418 2689 Size: 344 13 800 1572 3374 Fragments arranged by size: 3374 1572 800 344 13 ApaLI G'TGCA_C Cuts at: 4160 5316 4160 Size: 1156 4947 ApoI r'AATT_y Cuts at: 1493 3412 3638 3790 5899 1493 Size: 1919 226 152 2109 1697 Fragments arranged by size: 2109 1919 1697 226 152 AscI GG'CGCG_CC Cuts at: 2182 2182 Size: 6103 AseI AT'TA_AT Cuts at: 160 1032 4657 160 Size: 872 3625 1606 Fragments arranged by size: 3625 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6103 AvaI C'yCGr_G Cuts at: 1870 1943 2265 2890 3428 1870 Size: 73 322 625 538 4545 Fragments arranged by size: 4545 625 538 322 73 BamHI G'GATC_C Cuts at: 3433 3800 3433 Size: 367 5736 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2175 2504 2523 2894 2959 Size: 325 217 627 388 329 19 371 65 Cuts at: 2959 3056 3252 3424 4879 618 Size: 97 196 172 1455 1842 Fragments arranged by size: 1842 1455 627 388 371 329 325 217 196 172 97 65 19 BanII G_rGCy'C Cuts at: 1391 2219 2363 2710 3075 3394 3422 1391 Size: 828 144 347 365 319 28 4072 Fragments arranged by size: 4072 828 365 347 319 144 28 BbeI G_GCGC'C Cuts at: 2179 3060 2179 Size: 881 5222 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2128 928 Size: 971 229 4903 Fragments arranged by size: 4903 971 229 BbvCI CC'TCA_GC Cuts at: 2822 2822 Size: 6103 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4294 4328 1903 Size: 34 2357 34 3678 Fragments arranged by size: 3678 2357 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3995 5205 3995 Size: 1210 4893 BclI T'GATC_A Cuts at: 1548 2730 1548 Size: 1182 4921 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4714 136 Size: 107 122 71 631 605 3042 1525 Fragments arranged by size: 3042 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2271 6098 2271 Size: 3827 2276 BlpI GC'TnA_GC Cuts at: 3520 3520 Size: 6103 Bme1580I G_kGCm'C Cuts at: 1790 2507 2663 2897 2964 3093 3248 4164 5320 Size: 717 156 234 67 129 155 916 1156 Cuts at: 5320 1790 Size: 2573 Fragments arranged by size: 2573 1156 916 717 234 156 155 129 67 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6103 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3322 3834 4792 Size: 416 53 83 399 24 1905 512 958 Cuts at: 4792 442 Size: 1753 Fragments arranged by size: 1905 1753 958 512 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6103 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2266 2554 2266 Size: 288 5815 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2219 2243 2363 2806 3089 4763 1239 Size: 980 24 120 443 283 1674 2579 Fragments arranged by size: 2579 1674 980 443 283 120 24 Bpu10I CC'TnA_GC Cuts at: 2079 2822 2079 Size: 743 5360 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2323 4228 5093 5391 2323 Size: 1905 865 298 3035 Fragments arranged by size: 3035 1905 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2014 2755 3328 4766 882 Size: 617 515 741 573 1438 2219 Fragments arranged by size: 2219 1438 741 617 573 515 BsaAI yAC'GTr Cuts at: 493 1204 2883 3249 3894 493 Size: 711 1679 366 645 2702 Fragments arranged by size: 2702 1679 711 645 366 BsaBI GATnn'nnATC Cuts at: 6097 6097 Size: 6103 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2163 2176 3057 3910 Size: 53 83 186 936 630 13 881 853 Cuts at: 3910 4292 4927 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 881 853 635 630 382 186 83 53 13 BsaWI w'CCGG_w Cuts at: 4536 4934 5208 5355 5766 4536 Size: 398 274 147 411 4873 Fragments arranged by size: 4873 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2216 2231 2246 2261 3149 Size: 30 165 30 813 15 15 15 888 Cuts at: 3149 3179 1178 Size: 30 4102 Fragments arranged by size: 4102 888 813 165 30 30 30 15 15 15 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2018 2106 2216 2255 2273 1186 Size: 832 88 110 39 18 5016 Fragments arranged by size: 5016 832 110 88 39 18 BseYI C'CCAG_C Cuts at: 2806 2876 3033 3273 5306 5802 2806 Size: 70 157 240 2033 496 3107 Fragments arranged by size: 3107 2033 496 240 157 70 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2153 2548 2912 1163 Size: 573 417 395 364 4354 Fragments arranged by size: 4354 573 417 395 364 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2213 3553 4314 5342 1540 Size: 275 186 212 1340 761 1028 2301 Fragments arranged by size: 2301 1340 1028 761 275 212 186 BsiHKAI G_wGCw'C Cuts at: 2252 2710 2817 2832 3075 4164 4249 5320 2252 Size: 458 107 15 243 1089 85 1071 3035 Fragments arranged by size: 3035 1089 1071 458 243 107 85 15 BsiWI C'GTAC_G Cuts at: 2883 2883 Size: 6103 BsmI GAATG_Cn' Cuts at: 3614 3707 3614 Size: 93 6010 BsmBI CGTCTCn'nnnn_ Cuts at: 2423 2423 Size: 6103 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2219 2252 2363 2507 2663 2710 2817 Size: 399 429 33 111 144 156 47 107 Cuts at: 2817 2832 2897 2964 3075 3093 3248 3394 3422 Size: 15 65 67 111 18 155 146 28 Cuts at: 3422 4164 4249 5320 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 429 399 156 155 146 144 111 111 107 85 67 65 47 33 28 18 15 BspEI T'CCGG_A Cuts at: 5766 5766 Size: 6103 BspHI T'CATG_A Cuts at: 3992 5722 3992 Size: 1730 4373 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2674 2854 3458 844 Size: 1830 180 604 3489 Fragments arranged by size: 3489 1830 604 180 BsrBI CCG'CTC Cuts at: 1394 1823 3990 1394 Size: 429 2167 3507 Fragments arranged by size: 3507 2167 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4598 4772 65 Size: 1183 3350 174 1396 Fragments arranged by size: 3350 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2172 2178 2850 3173 4747 4934 1700 Size: 110 362 6 672 323 1574 187 2869 Fragments arranged by size: 2869 1574 672 362 323 187 110 6 BsrGI T'GTAC_A Cuts at: 96 3312 96 Size: 3216 2887 BssHII G'CGCG_C Cuts at: 1925 2182 1925 Size: 257 5846 BssSI C'ACGA_G Cuts at: 2528 4163 5175 2528 Size: 1635 1012 3456 Fragments arranged by size: 3456 1635 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2658 794 Size: 1864 4239 BstBI TT'CG_AA Cuts at: 4907 4907 Size: 6103 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6103 BstXI CCAn_nnnn'nTGG Cuts at: 2746 3067 2746 Size: 321 5782 BstYI r'GATC_y Cuts at: 1972 2271 3433 3800 4185 4202 5654 5740 5752 Size: 299 1162 367 385 17 1452 86 12 Cuts at: 5752 6098 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1162 385 367 346 299 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2021 2086 2021 Size: 65 6038 BtgI C'CryG_G Cuts at: 513 1065 2004 2111 2158 2678 2754 2937 3052 Size: 552 939 107 47 520 76 183 115 Cuts at: 3052 3060 3294 513 Size: 8 234 3322 Fragments arranged by size: 3322 939 552 520 234 183 115 107 76 47 8 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2896 487 Size: 683 56 1670 3694 Fragments arranged by size: 3694 1670 683 56 BtsI GCAGTG_nn' Cuts at: 2252 2497 3619 4413 4433 2252 Size: 245 1122 794 20 3922 Fragments arranged by size: 3922 1122 794 245 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6103 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6068 DraI TTT'AAA Cuts at: 3407 3407 Size: 6103 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5110 809 Size: 507 3794 1802 Fragments arranged by size: 3794 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2210 3550 4439 Size: 54 1425 50 275 18 380 1340 889 Cuts at: 4439 6068 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1340 889 380 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2210 3550 1537 Size: 275 398 1340 4090 Fragments arranged by size: 4090 1340 398 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4033 1353 Size: 2680 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2912 4690 5064 5210 387 Size: 1064 138 1323 1778 374 146 1280 Fragments arranged by size: 1778 1323 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2708 3073 2708 Size: 365 5738 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2033 2219 2243 2363 2806 3089 4166 Size: 393 401 186 24 120 443 283 1077 Cuts at: 4166 4763 5550 5818 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1077 787 597 443 401 393 283 268 186 120 24 EcoO109I rG'GnC_Cy Cuts at: 2017 2123 2686 2724 2017 Size: 106 563 38 5396 Fragments arranged by size: 5396 563 106 38 EcoRI G'AATT_C Cuts at: 3412 3790 3412 Size: 378 5725 FspI TGC'GCA Cuts at: 3289 4609 3289 Size: 1320 4783 HaeII r_GCGC'y Cuts at: 1678 2179 2515 3060 5250 1678 Size: 501 336 545 2190 2531 Fragments arranged by size: 2531 2190 545 501 336 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2543 2575 4744 4776 4818 Size: 32 135 32 971 32 2169 32 42 Cuts at: 4818 4850 1373 Size: 32 2626 Fragments arranged by size: 2626 2169 971 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3447 3693 669 Size: 988 281 1509 246 3079 Fragments arranged by size: 3079 1509 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6103 HpaI GTT'AAC Cuts at: 3693 3693 Size: 6103 KasI G'GCGC_C Cuts at: 2175 3056 2175 Size: 881 5222 KpnI G_GTAC'C Cuts at: 3256 3428 3256 Size: 172 5931 MfeI C'AATT_G Cuts at: 3702 3702 Size: 6103 MluI A'CGCG_T Cuts at: 2190 5979 2190 Size: 3789 2314 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5217 5401 1305 Size: 3912 184 2007 Fragments arranged by size: 3912 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4281 Fragments arranged by size: 4281 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2439 2744 4061 4420 4579 518 Size: 648 1273 305 1317 359 159 2042 Fragments arranged by size: 2042 1317 1273 648 359 305 159 MspA1I CmG'CkG Cuts at: 1606 2449 2756 3030 3043 3273 3519 4196 5344 Size: 843 307 274 13 230 246 677 1148 Cuts at: 5344 5589 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 843 677 307 274 246 245 230 13 NaeI GCC'GGC Cuts at: 2180 2852 3175 2180 Size: 672 323 5108 Fragments arranged by size: 5108 672 323 NarI GG'CG_CC Cuts at: 2176 3057 2176 Size: 881 5222 NcoI C'CATG_G Cuts at: 513 1065 2004 2678 513 Size: 552 939 674 3938 Fragments arranged by size: 3938 939 674 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6103 NgoMIV G'CCGG_C Cuts at: 2178 2850 3173 2178 Size: 672 323 5108 Fragments arranged by size: 5108 672 323 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6103 NotI GC'GGCC_GC Cuts at: 3550 3550 Size: 6103 NspI r_CATG'y Cuts at: 1132 1229 2110 3166 3230 3461 5006 1132 Size: 97 881 1056 64 231 1545 2229 Fragments arranged by size: 2229 1545 1056 881 231 97 64 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6103 PciI A'CATG_T Cuts at: 3162 5002 3162 Size: 1840 4263 PflMI CCAn_nnn'nTGG Cuts at: 1108 2938 3046 1108 Size: 1830 108 4165 Fragments arranged by size: 4165 1830 108 PfoI T'CCnGG_A Cuts at: 2196 2196 Size: 6103 PmeI GTTT'AAAC Cuts at: 3407 3407 Size: 6103 PmlI CAC'GTG Cuts at: 3249 3249 Size: 6103 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3149 3181 4172 4204 5685 5717 3149 Size: 32 991 32 1481 32 3535 Fragments arranged by size: 3535 1481 991 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2686 2724 2686 Size: 38 6065 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6103 PsiI TTA'TAA Cuts at: 3673 3673 Size: 6103 PstI C_TGCA'G Cuts at: 830 2068 2492 3120 3455 830 Size: 1238 424 628 335 3478 Fragments arranged by size: 3478 1238 628 424 335 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6103 PvuII CAG'CTG Cuts at: 2449 3043 3273 2449 Size: 594 230 5279 Fragments arranged by size: 5279 594 230 SacI G_AGCT'C Cuts at: 2710 3075 2710 Size: 365 5738 SacII CC_GC'GG Cuts at: 2757 2757 Size: 6103 SalI G'TCGA_C Cuts at: 1936 3445 1936 Size: 1509 4594 SanDI GG'GwC_CC Cuts at: 2724 2724 Size: 6103 SbfI CC_TGCA'GG Cuts at: 3455 3455 Size: 6103 ScaI AGT'ACT Cuts at: 2486 4351 6063 2486 Size: 1865 1712 2526 Fragments arranged by size: 2526 1865 1712 SfcI C'TryA_G Cuts at: 826 1045 2064 2488 3116 3451 4586 5267 5458 Size: 219 1019 424 628 335 1135 681 191 Cuts at: 5458 826 Size: 1471 Fragments arranged by size: 1471 1135 1019 681 628 424 335 219 191 SfoI GGC'GCC Cuts at: 2177 3058 2177 Size: 881 5222 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6103 SgrAI Cr'CCGG_yG Cuts at: 1700 2172 2178 1700 Size: 472 6 5625 Fragments arranged by size: 5625 472 6 SmaI CCC'GGG Cuts at: 1872 2267 2892 3430 1872 Size: 395 625 538 4545 Fragments arranged by size: 4545 625 538 395 SmlI C'TyrA_G Cuts at: 820 1017 1943 2338 4207 5108 5370 820 Size: 197 926 395 1869 901 262 1553 Fragments arranged by size: 1869 1553 926 901 395 262 197 SnaBI TAC'GTA Cuts at: 493 3894 493 Size: 3401 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6103 SphI G_CATG'C Cuts at: 1132 3461 1132 Size: 2329 3774 SspI AAT'ATT Cuts at: 5 52 4027 5 Size: 47 3975 2081 Fragments arranged by size: 3975 2081 47 StuI AGG'CCT Cuts at: 1517 2084 1517 Size: 567 5536 StyI C'CwwG_G Cuts at: 513 1065 2004 2632 2678 3542 513 Size: 552 939 628 46 864 3074 Fragments arranged by size: 3074 939 864 628 552 46 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2584 2734 3248 4130 4147 4300 4485 Size: 1255 1004 150 514 882 17 153 185 Cuts at: 4485 4930 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1004 882 514 445 185 153 150 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2484 3159 3312 Size: 274 80 33 51 1443 507 675 153 Cuts at: 3312 3330 4349 6061 96 Size: 18 1019 1712 138 Fragments arranged by size: 1712 1443 1019 675 507 274 153 138 80 51 33 18 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2096 2128 2216 2231 2248 2263 6063 6095 2096 Size: 32 88 15 17 15 3800 32 2104 Fragments arranged by size: 3800 2104 88 32 32 17 15 15 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6103 XbaI T'CTAG_A Cuts at: 2605 3439 2605 Size: 834 5269 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2194 2194 Size: 6103 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6103 XmaI C'CCGG_G Cuts at: 1870 2265 2890 3428 1870 Size: 395 625 538 4545 Fragments arranged by size: 4545 625 538 395 XmnI GAAnn'nnTTC Cuts at: 2300 4232 2300 Size: 1932 4171 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3911 276 Size: 53 83 186 936 2377 2468 Fragments arranged by size: 2468 2377 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SacII SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI ApaI AvrII BaeI BfrBI BstZ17I DraIII EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PspOMI PspXI PsrI RsrII SapI SexAI SfiI SrfI SwaI