(Circular) (Six-base) MAPSORT of: pFN21AE0986.seq Check: 1838 from: 1 to: 6508 pFN21AE0986 6508 bp With 182 enzymes: SgfI * April 15, 2010 09:34 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2244 2629 2698 2829 2244 Size: 385 69 131 5923 Fragments arranged by size: 5923 385 131 69 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4318 278 Size: 53 83 186 936 2782 2468 Fragments arranged by size: 2782 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3141 3851 1937 Size: 1204 710 4594 Fragments arranged by size: 4594 1204 710 Acc65I G'GTAC_C Cuts at: 3829 3829 Size: 6508 AclI AA'CG_TT Cuts at: 1563 4635 5008 1563 Size: 3072 373 3063 Fragments arranged by size: 3072 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2069 2947 3524 4571 5955 6223 1632 Size: 437 878 577 1047 1384 268 1917 Fragments arranged by size: 1917 1384 1047 878 577 437 268 AfeI AGC'GCT Cuts at: 2777 2777 Size: 6508 AflII C'TTAA_G Cuts at: 820 1017 3050 820 Size: 197 2033 4278 Fragments arranged by size: 4278 2033 197 AflIII A'CryG_T Cuts at: 1203 3144 5407 6384 1203 Size: 1941 2263 977 1327 Fragments arranged by size: 2263 1941 1327 977 AgeI A'CCGG_T Cuts at: 3674 5339 3674 Size: 1665 4843 AhdI GACnn_n'nnGTC Cuts at: 5237 5237 Size: 6508 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6508 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6508 AlwNI CAG_nnn'CTG Cuts at: 3337 4251 5823 3337 Size: 914 1572 4022 Fragments arranged by size: 4022 1572 914 ApaI G_GGCC'C Cuts at: 2598 3473 2598 Size: 875 5633 ApaLI G'TGCA_C Cuts at: 4565 5721 4565 Size: 1156 5352 ApoI r'AATT_y Cuts at: 1493 2872 2951 3364 3817 4043 4195 6304 1493 Size: 1379 79 413 453 226 152 2109 1697 Fragments arranged by size: 2109 1697 1379 453 413 226 152 79 AseI AT'TA_AT Cuts at: 160 1032 5062 160 Size: 872 4030 1606 Fragments arranged by size: 4030 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6508 AvaI C'yCGr_G Cuts at: 1870 1943 2095 3056 3833 1870 Size: 73 152 961 777 4545 Fragments arranged by size: 4545 961 777 152 73 BamHI G'GATC_C Cuts at: 3559 3838 4205 3559 Size: 279 367 5862 Fragments arranged by size: 5862 367 279 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2368 2641 3597 3829 5284 Size: 325 217 627 581 273 956 232 1455 Cuts at: 5284 618 Size: 1842 Fragments arranged by size: 1842 1455 956 627 581 325 273 232 217 BanII G_rGCy'C Cuts at: 1391 2103 2414 2598 2937 3175 3249 3377 3473 Size: 712 311 184 339 238 74 128 96 Cuts at: 3473 3827 1391 Size: 354 4072 Fragments arranged by size: 4072 712 354 339 311 238 184 128 96 74 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5537 BbvCI CC'TCA_GC Cuts at: 3608 3608 Size: 6508 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4699 4733 1903 Size: 34 2762 34 3678 Fragments arranged by size: 3678 2762 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4400 5610 4400 Size: 1210 5298 BclI T'GATC_A Cuts at: 1548 2230 1548 Size: 682 5826 BfrBI ATG'CAT Cuts at: 3347 3347 Size: 6508 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2377 5119 136 Size: 107 122 71 631 605 705 2742 1525 Fragments arranged by size: 2742 1525 705 631 605 122 107 71 BglII A'GATC_T Cuts at: 6503 6503 Size: 6508 BlpI GC'TnA_GC Cuts at: 2915 3718 3925 2915 Size: 803 207 5498 Fragments arranged by size: 5498 803 207 Bme1580I G_kGCm'C Cuts at: 1790 2598 2646 3473 4569 5725 1790 Size: 808 48 827 1096 1156 2573 Fragments arranged by size: 2573 1156 1096 827 808 48 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6508 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4239 5197 442 Size: 416 53 83 399 24 2822 958 1753 Fragments arranged by size: 2822 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6508 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2888 3100 3159 5168 1239 Size: 1649 212 59 2009 2579 Fragments arranged by size: 2579 2009 1649 212 59 Bpu10I CC'TnA_GC Cuts at: 3608 3608 Size: 6508 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2663 4633 5498 5796 2663 Size: 1970 865 298 3375 Fragments arranged by size: 3375 1970 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5171 882 Size: 617 3672 2219 Fragments arranged by size: 3672 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2656 2746 3147 4299 493 Size: 711 1452 90 401 1152 2702 Fragments arranged by size: 2702 1452 1152 711 401 90 BsaBI GATnn'nnATC Cuts at: 2807 6502 2807 Size: 3695 2813 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4315 4697 5332 275 Size: 53 83 186 936 2782 382 635 1451 Fragments arranged by size: 2782 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3475 3674 4941 5339 5613 5760 6171 3475 Size: 199 1267 398 274 147 411 3812 Fragments arranged by size: 3812 1267 411 398 274 199 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3632 3662 1178 Size: 30 165 30 2229 30 4024 Fragments arranged by size: 4024 2229 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2813 3625 1186 Size: 1627 812 4069 Fragments arranged by size: 4069 1627 812 BseYI C'CCAG_C Cuts at: 5711 6207 5711 Size: 496 6012 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2127 2657 3382 1163 Size: 573 391 530 725 4289 Fragments arranged by size: 4289 725 573 530 391 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2028 2052 2056 3958 4719 5747 Size: 275 186 27 24 4 1902 761 1028 Cuts at: 5747 1540 Size: 2301 Fragments arranged by size: 2301 1902 1028 761 275 186 27 24 4 BsiHKAI G_wGCw'C Cuts at: 2316 2414 2937 3249 3377 3674 4569 4654 5725 Size: 98 523 312 128 297 895 85 1071 Cuts at: 5725 2316 Size: 3099 Fragments arranged by size: 3099 1071 895 523 312 297 128 98 85 BsmI GAATG_Cn' Cuts at: 2391 4019 4112 2391 Size: 1628 93 4787 Fragments arranged by size: 4787 1628 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2103 2316 2414 2598 2646 2937 3175 Size: 399 313 213 98 184 48 291 238 Cuts at: 3175 3249 3377 3473 3674 3827 4569 4654 5725 Size: 74 128 96 201 153 742 85 1071 Cuts at: 5725 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 399 313 291 238 213 201 184 153 128 98 96 85 74 48 BspEI T'CCGG_A Cuts at: 6171 6171 Size: 6508 BspHI T'CATG_A Cuts at: 4397 6127 4397 Size: 1730 4778 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2244 2629 2698 2829 3863 844 Size: 1400 385 69 131 1034 3489 Fragments arranged by size: 3489 1400 1034 385 131 69 BsrBI CCG'CTC Cuts at: 1394 1823 2033 2124 2377 4395 1394 Size: 429 210 91 253 2018 3507 Fragments arranged by size: 3507 2018 429 253 210 91 BsrDI GCAATG_nn' Cuts at: 65 1248 2487 5003 5177 65 Size: 1183 1239 2516 174 1396 Fragments arranged by size: 2516 1396 1239 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2051 2784 3674 5152 5339 1700 Size: 110 241 733 890 1478 187 2869 Fragments arranged by size: 2869 1478 890 733 241 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6508 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6508 BssSI C'ACGA_G Cuts at: 4568 5580 4568 Size: 1012 5496 BstAPI GCAn_nnn'nTGC Cuts at: 794 2494 794 Size: 1700 4808 BstBI TT'CG_AA Cuts at: 2514 5312 2514 Size: 2798 3710 BstEII G'GTnAC_C Cuts at: 1184 3257 1184 Size: 2073 4435 BstXI CCAn_nnnn'nTGG Cuts at: 2204 2204 Size: 6508 BstYI r'GATC_y Cuts at: 1972 2265 2802 3559 3838 4205 4590 4607 6059 Size: 293 537 757 279 367 385 17 1452 Cuts at: 6059 6145 6157 6503 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 757 537 385 367 346 293 279 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2430 513 Size: 552 1365 4591 Fragments arranged by size: 4591 1365 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2137 487 Size: 683 56 911 4858 Fragments arranged by size: 4858 911 683 56 BtsI GCAGTG_nn' Cuts at: 3011 3573 4024 4818 4838 3011 Size: 562 451 794 20 4681 Fragments arranged by size: 4681 794 562 451 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6508 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2587 2622 529 Size: 35 2023 35 4415 Fragments arranged by size: 4415 2023 35 35 DraI TTT'AAA Cuts at: 3812 3812 Size: 6508 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5515 809 Size: 507 4199 1802 Fragments arranged by size: 4199 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2025 2053 3955 Size: 54 1425 50 275 18 195 28 1902 Cuts at: 3955 4844 6473 8 Size: 889 1629 43 Fragments arranged by size: 1902 1629 1425 889 275 195 54 50 43 28 18 EagI C'GGCC_G Cuts at: 1537 1812 2025 2053 3955 1537 Size: 275 213 28 1902 4090 Fragments arranged by size: 4090 1902 275 213 28 EarI CTCTTCn'nnn_ Cuts at: 1353 4438 1353 Size: 3085 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5095 5469 5615 387 Size: 1064 138 3506 374 146 1280 Fragments arranged by size: 3506 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2412 2935 3247 3375 2412 Size: 523 312 128 5545 Fragments arranged by size: 5545 523 312 128 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2069 2888 2947 3100 3159 3524 4571 Size: 393 437 819 59 153 59 365 1047 Cuts at: 4571 5168 5955 6223 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1047 819 787 597 437 393 365 268 153 59 59 EcoNI CCTnn'n_nnAGG Cuts at: 3607 3607 Size: 6508 EcoO109I rG'GnC_Cy Cuts at: 2674 3469 2674 Size: 795 5713 EcoRI G'AATT_C Cuts at: 3817 4195 3817 Size: 378 6130 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2716 3203 2716 Size: 487 6021 FspI TGC'GCA Cuts at: 3572 5014 3572 Size: 1442 5066 FspAI rTGC'GCAy Cuts at: 3572 3572 Size: 6508 HaeII r_GCGC'y Cuts at: 1678 2073 2578 2779 5655 1678 Size: 395 505 201 2876 2531 Fragments arranged by size: 2876 2531 505 395 201 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2612 2644 3098 3130 3632 Size: 32 135 32 1040 32 454 32 502 Cuts at: 3632 3664 5149 5181 5223 5255 1373 Size: 32 1485 32 42 32 2626 Fragments arranged by size: 2626 1485 1040 502 454 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2010 3852 4098 669 Size: 988 281 72 1842 246 3079 Fragments arranged by size: 3079 1842 988 281 246 72 HindIII A'AGCT_T Cuts at: 748 748 Size: 6508 HpaI GTT'AAC Cuts at: 2010 4098 2010 Size: 2088 4420 KpnI G_GTAC'C Cuts at: 3833 3833 Size: 6508 MfeI C'AATT_G Cuts at: 4107 4107 Size: 6508 MluI A'CGCG_T Cuts at: 6384 6384 Size: 6508 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5622 5806 1305 Size: 4317 184 2007 Fragments arranged by size: 4317 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4686 Fragments arranged by size: 4686 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2238 2462 2861 2897 3135 3215 4466 Size: 648 1072 224 399 36 238 80 1251 Cuts at: 4466 4825 4984 518 Size: 359 159 2042 Fragments arranged by size: 2042 1251 1072 648 399 359 238 224 159 80 36 MspA1I CmG'CkG Cuts at: 1606 2383 2795 3295 3924 4601 5749 5994 1606 Size: 777 412 500 629 677 1148 245 2120 Fragments arranged by size: 2120 1148 777 677 629 500 412 245 NcoI C'CATG_G Cuts at: 513 1065 2430 513 Size: 552 1365 4591 Fragments arranged by size: 4591 1365 552 NdeI CA'TA_TG Cuts at: 387 2632 2862 387 Size: 2245 230 4033 Fragments arranged by size: 4033 2245 230 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6508 NotI GC'GGCC_GC Cuts at: 3955 3955 Size: 6508 NsiI A_TGCA'T Cuts at: 3349 3349 Size: 6508 NspI r_CATG'y Cuts at: 1132 1229 2243 2497 3208 3325 3866 5411 1132 Size: 97 1014 254 711 117 541 1545 2229 Fragments arranged by size: 2229 1545 1014 711 541 254 117 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6508 PciI A'CATG_T Cuts at: 5407 5407 Size: 6508 PflMI CCAn_nnn'nTGG Cuts at: 1108 2746 2866 1108 Size: 1638 120 4750 Fragments arranged by size: 4750 1638 120 PfoI T'CCnGG_A Cuts at: 2811 3449 2811 Size: 638 5870 PmeI GTTT'AAAC Cuts at: 3812 3812 Size: 6508 PmlI CAC'GTG Cuts at: 2656 2746 2656 Size: 90 6418 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4577 4609 6090 6122 4577 Size: 32 1481 32 4963 Fragments arranged by size: 4963 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2674 2674 Size: 6508 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6508 PsiI TTA'TAA Cuts at: 4078 4078 Size: 6508 PspOMI G'GGCC_C Cuts at: 2594 3469 2594 Size: 875 5633 PstI C_TGCA'G Cuts at: 830 3006 3568 3860 830 Size: 2176 562 292 3478 Fragments arranged by size: 3478 2176 562 292 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6508 PvuII CAG'CTG Cuts at: 2383 3295 2383 Size: 912 5596 SacI G_AGCT'C Cuts at: 2414 2937 3249 3377 2414 Size: 523 312 128 5545 Fragments arranged by size: 5545 523 312 128 SalI G'TCGA_C Cuts at: 1936 3850 1936 Size: 1914 4594 SbfI CC_TGCA'GG Cuts at: 3860 3860 Size: 6508 ScaI AGT'ACT Cuts at: 4756 6468 4756 Size: 1712 4796 SfcI C'TryA_G Cuts at: 826 1045 3002 3564 3856 4991 5672 5863 826 Size: 219 1957 562 292 1135 681 191 1471 Fragments arranged by size: 1957 1471 1135 681 562 292 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6508 SgrAI Cr'CCGG_yG Cuts at: 1700 3674 1700 Size: 1974 4534 SmaI CCC'GGG Cuts at: 1872 2097 3835 1872 Size: 225 1738 4545 Fragments arranged by size: 4545 1738 225 SmlI C'TyrA_G Cuts at: 820 1017 1943 2678 3050 4612 5513 5775 820 Size: 197 926 735 372 1562 901 262 1553 Fragments arranged by size: 1562 1553 926 901 735 372 262 197 SnaBI TAC'GTA Cuts at: 493 4299 493 Size: 3806 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6508 SphI G_CATG'C Cuts at: 1132 3208 3325 3866 1132 Size: 2076 117 541 3774 Fragments arranged by size: 3774 2076 541 117 SspI AAT'ATT Cuts at: 5 52 3772 4432 5 Size: 47 3720 660 2081 Fragments arranged by size: 3720 2081 660 47 StuI AGG'CCT Cuts at: 1517 2720 1517 Size: 1203 5305 StyI C'CwwG_G Cuts at: 513 1065 2430 3187 3378 3947 513 Size: 552 1365 757 191 569 3074 Fragments arranged by size: 3074 1365 757 569 552 191 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4535 4552 4705 4890 5335 325 Size: 1255 2955 17 153 185 445 1498 Fragments arranged by size: 2955 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4754 6466 96 Size: 274 80 33 51 1443 2777 1712 138 Fragments arranged by size: 2777 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2619 2651 6468 6500 2619 Size: 32 3817 32 2627 Fragments arranged by size: 3817 2627 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6508 XbaI T'CTAG_A Cuts at: 3844 3844 Size: 6508 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2437 2437 Size: 6508 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6508 XmaI C'CCGG_G Cuts at: 1870 2095 3833 1870 Size: 225 1738 4545 Fragments arranged by size: 4545 1738 225 XmnI GAAnn'nnTTC Cuts at: 2777 2951 4637 2777 Size: 174 1686 4648 Fragments arranged by size: 4648 1686 174 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4316 276 Size: 53 83 186 936 2782 2468 Fragments arranged by size: 2782 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbeI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FseI KasI NaeI NarI NgoMIV NruI PacI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI