(Circular) (Six-base) MAPSORT of: pFN21AE0728.seq Check: 2520 from: 1 to: 5959 pFN21AE0728 5959 bp With 182 enzymes: SgfI * April 15, 2010 09:34 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2886 2886 Size: 5959 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3769 278 Size: 53 83 186 936 2233 2468 Fragments arranged by size: 2468 2233 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2718 3302 1937 Size: 781 584 4594 Fragments arranged by size: 4594 781 584 Acc65I G'GTAC_C Cuts at: 3280 3280 Size: 5959 AclI AA'CG_TT Cuts at: 1563 4086 4459 1563 Size: 2523 373 3063 Fragments arranged by size: 3063 2523 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2425 2896 4022 5406 5674 1632 Size: 793 471 1126 1384 268 1917 Fragments arranged by size: 1917 1384 1126 793 471 268 AfeI AGC'GCT Cuts at: 2308 2308 Size: 5959 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5762 AflIII A'CryG_T Cuts at: 1203 2644 4858 5835 1203 Size: 1441 2214 977 1327 Fragments arranged by size: 2214 1441 1327 977 AgeI A'CCGG_T Cuts at: 4790 4790 Size: 5959 AhdI GACnn_n'nnGTC Cuts at: 4688 4688 Size: 5959 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5959 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3005 1184 Size: 1821 4138 AlwNI CAG_nnn'CTG Cuts at: 2111 3702 5274 2111 Size: 1591 1572 2796 Fragments arranged by size: 2796 1591 1572 ApaLI G'TGCA_C Cuts at: 4016 5172 4016 Size: 1156 4803 ApoI r'AATT_y Cuts at: 1493 2729 3268 3494 3646 5755 1493 Size: 1236 539 226 152 2109 1697 Fragments arranged by size: 2109 1697 1236 539 226 152 AseI AT'TA_AT Cuts at: 160 1032 4513 160 Size: 872 3481 1606 Fragments arranged by size: 3481 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5959 AvaI C'yCGr_G Cuts at: 1870 1943 2240 3284 1870 Size: 73 297 1044 4545 Fragments arranged by size: 4545 1044 297 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3229 3262 3229 Size: 33 5926 BamHI G'GATC_C Cuts at: 2452 3289 3656 2452 Size: 837 367 4755 Fragments arranged by size: 4755 837 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2324 2597 2923 3131 3280 Size: 325 217 627 537 273 326 208 149 Cuts at: 3280 4735 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 537 326 325 273 217 208 149 BanII G_rGCy'C Cuts at: 1391 2294 3278 1391 Size: 903 984 4072 Fragments arranged by size: 4072 984 903 BbeI G_GCGC'C Cuts at: 2927 2927 Size: 5959 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4988 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2543 2577 2717 2751 4150 Size: 34 50 34 522 34 140 34 1399 Cuts at: 4150 4184 1903 Size: 34 3678 Fragments arranged by size: 3678 1399 522 140 50 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2183 2847 3230 3851 5061 2183 Size: 664 383 621 1210 3081 Fragments arranged by size: 3081 1210 664 621 383 BclI T'GATC_A Cuts at: 1548 1548 Size: 5959 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2416 4570 Size: 107 122 71 631 605 337 407 2154 Cuts at: 4570 136 Size: 1525 Fragments arranged by size: 2154 1525 631 605 407 337 122 107 71 BglII A'GATC_T Cuts at: 5954 5954 Size: 5959 BlpI GC'TnA_GC Cuts at: 2130 3376 2130 Size: 1246 4713 Bme1580I G_kGCm'C Cuts at: 1790 2794 4020 5176 1790 Size: 1004 1226 1156 2573 Fragments arranged by size: 2573 1226 1156 1004 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5959 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2143 2790 3690 Size: 416 53 83 399 24 726 647 900 Cuts at: 3690 4648 442 Size: 958 1753 Fragments arranged by size: 1753 958 900 726 647 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5959 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2542 2542 Size: 5959 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3093 4619 1239 Size: 1854 1526 2579 Fragments arranged by size: 2579 1854 1526 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2001 4084 4949 5247 2001 Size: 2083 865 298 2713 Fragments arranged by size: 2713 2083 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3200 4622 882 Size: 617 1701 1422 2219 Fragments arranged by size: 2219 1701 1422 617 BsaAI yAC'GTr Cuts at: 493 1204 2645 3750 493 Size: 711 1441 1105 2702 Fragments arranged by size: 2702 1441 1105 711 BsaBI GATnn'nnATC Cuts at: 5953 5953 Size: 5959 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2012 2924 3766 4148 Size: 53 83 186 936 479 912 842 382 Cuts at: 4148 4783 275 Size: 635 1451 Fragments arranged by size: 1451 936 912 842 635 479 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2455 4392 4790 5064 5211 5622 2455 Size: 1937 398 274 147 411 2792 Fragments arranged by size: 2792 1937 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5734 Fragments arranged by size: 5734 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5959 BseYI C'CCAG_C Cuts at: 3024 5162 5658 3024 Size: 2138 496 3325 Fragments arranged by size: 3325 2138 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2733 1163 Size: 573 997 4389 Fragments arranged by size: 4389 997 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2859 2893 3409 4170 5198 1540 Size: 275 186 858 34 516 761 1028 2301 Fragments arranged by size: 2301 1028 858 761 516 275 186 34 BsiHKAI G_wGCw'C Cuts at: 2247 4020 4105 5176 2247 Size: 1773 85 1071 3030 Fragments arranged by size: 3030 1773 1071 85 BsmI GAATG_Cn' Cuts at: 3145 3470 3563 3145 Size: 325 93 5541 Fragments arranged by size: 5541 325 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2019 2323 2019 Size: 304 5655 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2247 2294 2794 3278 4020 4105 5176 Size: 399 457 47 500 484 742 85 1071 Cuts at: 5176 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 500 484 457 399 85 47 BspEI T'CCGG_A Cuts at: 2455 5622 2455 Size: 3167 2792 BspHI T'CATG_A Cuts at: 3848 5578 3848 Size: 1730 4229 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2886 3314 844 Size: 2042 428 3489 Fragments arranged by size: 3489 2042 428 BsrBI CCG'CTC Cuts at: 1394 1823 3846 1394 Size: 429 2023 3507 Fragments arranged by size: 3507 2023 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2826 3209 4454 4628 65 Size: 1183 1578 383 1245 174 1396 Fragments arranged by size: 1578 1396 1245 1183 383 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2059 4603 4790 1700 Size: 110 249 2544 187 2869 Fragments arranged by size: 2869 2544 249 187 110 BsrGI T'GTAC_A Cuts at: 96 2784 2901 96 Size: 2688 117 3154 Fragments arranged by size: 3154 2688 117 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5959 BssSI C'ACGA_G Cuts at: 2302 4019 5031 2302 Size: 1717 1012 3230 Fragments arranged by size: 3230 1717 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2111 794 Size: 1317 4642 BstBI TT'CG_AA Cuts at: 2553 4763 2553 Size: 2210 3749 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5959 BstYI r'GATC_y Cuts at: 1972 2452 2774 3289 3656 4041 4058 5510 5596 Size: 480 322 515 367 385 17 1452 86 Cuts at: 5596 5608 5954 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 515 480 385 367 346 322 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2178 2232 2601 2853 2887 2919 513 Size: 552 1113 54 369 252 34 32 3553 Fragments arranged by size: 3553 1113 552 369 252 54 34 32 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5220 Fragments arranged by size: 5220 683 56 BtsI GCAGTG_nn' Cuts at: 3039 3475 4269 4289 3039 Size: 436 794 20 4709 Fragments arranged by size: 4709 794 436 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5959 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5924 DraI TTT'AAA Cuts at: 3263 3263 Size: 5959 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4966 809 Size: 507 3650 1802 Fragments arranged by size: 3650 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2362 2856 3406 Size: 54 1425 50 275 18 532 494 550 Cuts at: 3406 4295 5924 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 550 532 494 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2856 3406 1537 Size: 275 1044 550 4090 Fragments arranged by size: 4090 1044 550 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3889 1353 Size: 2536 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2312 2967 4546 4920 5066 387 Size: 1064 138 723 655 1579 374 146 1280 Fragments arranged by size: 1579 1280 1064 723 655 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2425 2896 3093 4022 4619 5406 5674 Size: 393 793 471 197 929 597 787 268 Cuts at: 5674 1239 Size: 1524 Fragments arranged by size: 1524 929 793 787 597 471 393 268 197 EcoRI G'AATT_C Cuts at: 2729 3268 3646 2729 Size: 539 378 5042 Fragments arranged by size: 5042 539 378 FspI TGC'GCA Cuts at: 4465 4465 Size: 5959 HaeII r_GCGC'y Cuts at: 1678 2310 2927 5106 1678 Size: 632 617 2179 2531 Fragments arranged by size: 2531 2179 632 617 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4600 4632 4674 4706 1373 Size: 32 135 32 3028 32 42 32 2626 Fragments arranged by size: 3028 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2671 3303 3549 669 Size: 988 281 733 632 246 3079 Fragments arranged by size: 3079 988 733 632 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5959 HpaI GTT'AAC Cuts at: 3549 3549 Size: 5959 KasI G'GCGC_C Cuts at: 2923 2923 Size: 5959 KpnI G_GTAC'C Cuts at: 3284 3284 Size: 5959 MfeI C'AATT_G Cuts at: 3558 3558 Size: 5959 MluI A'CGCG_T Cuts at: 5835 5835 Size: 5959 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5073 5257 1305 Size: 3768 184 2007 Fragments arranged by size: 3768 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2364 10 Size: 54 1425 343 532 3605 Fragments arranged by size: 3605 1425 532 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2037 3183 3917 4276 4435 518 Size: 648 871 1146 734 359 159 2042 Fragments arranged by size: 2042 1146 871 734 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2111 2180 2855 2889 2921 3047 3375 4052 Size: 505 69 675 34 32 126 328 677 Cuts at: 4052 5200 5445 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 677 675 505 328 245 126 69 34 32 NarI GG'CG_CC Cuts at: 2924 2924 Size: 5959 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5407 NdeI CA'TA_TG Cuts at: 387 387 Size: 5959 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5959 NotI GC'GGCC_GC Cuts at: 2856 3406 2856 Size: 550 5409 NspI r_CATG'y Cuts at: 1132 1229 2426 3317 4862 1132 Size: 97 1197 891 1545 2229 Fragments arranged by size: 2229 1545 1197 891 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5959 PciI A'CATG_T Cuts at: 4858 4858 Size: 5959 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5959 PfoI T'CCnGG_A Cuts at: 2077 2448 2077 Size: 371 5588 PmeI GTTT'AAAC Cuts at: 3263 3263 Size: 5959 PmlI CAC'GTG Cuts at: 2645 2645 Size: 5959 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4028 4060 5541 5573 4028 Size: 32 1481 32 4414 Fragments arranged by size: 4414 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5959 PsiI TTA'TAA Cuts at: 3529 3529 Size: 5959 PstI C_TGCA'G Cuts at: 830 2997 3311 830 Size: 2167 314 3478 Fragments arranged by size: 3478 2167 314 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5959 SacII CC_GC'GG Cuts at: 2181 2856 2890 2922 2181 Size: 675 34 32 5218 Fragments arranged by size: 5218 675 34 32 SalI G'TCGA_C Cuts at: 1936 3301 1936 Size: 1365 4594 SbfI CC_TGCA'GG Cuts at: 3311 3311 Size: 5959 ScaI AGT'ACT Cuts at: 4207 5919 4207 Size: 1712 4247 SfcI C'TryA_G Cuts at: 826 1045 2719 2993 3052 3307 4442 5123 5314 Size: 219 1674 274 59 255 1135 681 191 Cuts at: 5314 826 Size: 1471 Fragments arranged by size: 1674 1471 1135 681 274 255 219 191 59 SfoI GGC'GCC Cuts at: 2925 2925 Size: 5959 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5959 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5959 SmaI CCC'GGG Cuts at: 1872 3286 1872 Size: 1414 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2016 4063 4964 5226 820 Size: 197 926 73 2047 901 262 1553 Fragments arranged by size: 2047 1553 926 901 262 197 73 SnaBI TAC'GTA Cuts at: 493 3750 493 Size: 3257 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5959 SphI G_CATG'C Cuts at: 1132 3317 1132 Size: 2185 3774 SspI AAT'ATT Cuts at: 5 52 3883 5 Size: 47 3831 2081 Fragments arranged by size: 3831 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5959 StyI C'CwwG_G Cuts at: 513 1065 3398 513 Size: 552 2333 3074 Fragments arranged by size: 3074 2333 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2699 3986 4003 4156 4341 4786 325 Size: 1255 1119 1287 17 153 185 445 1498 Fragments arranged by size: 1498 1287 1255 1119 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2217 2784 2901 Size: 274 80 33 51 1443 240 567 117 Cuts at: 2901 4205 5917 96 Size: 1304 1712 138 Fragments arranged by size: 1712 1443 1304 567 274 240 138 117 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5919 5951 5919 Size: 32 5927 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5959 XbaI T'CTAG_A Cuts at: 3295 3295 Size: 5959 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5959 XmaI C'CCGG_G Cuts at: 1870 3284 1870 Size: 1414 4545 XmnI GAAnn'nnTTC Cuts at: 4088 4088 Size: 5959 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3767 276 Size: 53 83 186 936 2233 2468 Fragments arranged by size: 2468 2233 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PstI PvuI SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BbvCI BfrBI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SanDI SapI SexAI SfiI SrfI SwaI XcmI