(Circular) (Six-base) MAPSORT of: pFN21AE0690.seq Check: 7213 from: 1 to: 5908 pFN21AE0690 5908 bp With 182 enzymes: SgfI * April 15, 2010 09:34 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3718 278 Size: 53 83 186 936 2182 2468 Fragments arranged by size: 2468 2182 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3251 1937 Size: 1314 4594 Acc65I G'GTAC_C Cuts at: 3229 3229 Size: 5908 AclI AA'CG_TT Cuts at: 1563 4035 4408 1563 Size: 2472 373 3063 Fragments arranged by size: 3063 2472 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2708 2898 3971 5355 5623 1632 Size: 1076 190 1073 1384 268 1917 Fragments arranged by size: 1917 1384 1076 1073 268 190 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5711 AflIII A'CryG_T Cuts at: 1203 3197 4807 5784 1203 Size: 1994 1610 977 1327 Fragments arranged by size: 1994 1610 1327 977 AgeI A'CCGG_T Cuts at: 4739 4739 Size: 5908 AhdI GACnn_n'nnGTC Cuts at: 4637 4637 Size: 5908 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5908 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5908 AlwNI CAG_nnn'CTG Cuts at: 3651 5223 3651 Size: 1572 4336 ApaI G_GGCC'C Cuts at: 2284 2356 2284 Size: 72 5836 ApaLI G'TGCA_C Cuts at: 2675 3965 5121 2675 Size: 1290 1156 3462 Fragments arranged by size: 3462 1290 1156 ApoI r'AATT_y Cuts at: 1493 2345 3217 3443 3595 5704 1493 Size: 852 872 226 152 2109 1697 Fragments arranged by size: 2109 1697 872 852 226 152 AseI AT'TA_AT Cuts at: 160 1032 4462 160 Size: 872 3430 1606 Fragments arranged by size: 3430 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5908 AvaI C'yCGr_G Cuts at: 1870 1943 2115 2146 2283 2318 2784 3233 1870 Size: 73 172 31 137 35 466 449 4545 Fragments arranged by size: 4545 466 449 172 137 73 35 31 BamHI G'GATC_C Cuts at: 2125 2505 3238 3605 2125 Size: 380 733 367 4428 Fragments arranged by size: 4428 733 380 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2024 2080 2373 2723 2779 Size: 325 217 627 237 56 293 350 56 Cuts at: 2779 3229 4684 618 Size: 450 1455 1842 Fragments arranged by size: 1842 1455 627 450 350 325 293 237 217 56 56 BanII G_rGCy'C Cuts at: 1391 2284 2356 2459 2529 2991 3227 1391 Size: 893 72 103 70 462 236 4072 Fragments arranged by size: 4072 893 462 236 103 72 70 BbeI G_GCGC'C Cuts at: 2028 2084 2377 2028 Size: 56 293 5559 Fragments arranged by size: 5559 293 56 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4937 BbvCI CC'TCA_GC Cuts at: 2096 2520 2845 2096 Size: 424 325 5159 Fragments arranged by size: 5159 424 325 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4099 4133 1903 Size: 34 2162 34 3678 Fragments arranged by size: 3678 2162 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3800 5010 3800 Size: 1210 4698 BclI T'GATC_A Cuts at: 1548 1548 Size: 5908 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2162 2288 4519 Size: 107 122 71 631 605 490 126 2231 Cuts at: 4519 136 Size: 1525 Fragments arranged by size: 2231 1525 631 605 490 126 122 107 71 BglII A'GATC_T Cuts at: 5903 5903 Size: 5908 BlpI GC'TnA_GC Cuts at: 3325 3325 Size: 5908 Bme1580I G_kGCm'C Cuts at: 1790 2284 2356 2679 3969 5125 1790 Size: 494 72 323 1290 1156 2573 Fragments arranged by size: 2573 1290 1156 494 323 72 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5908 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2063 3639 4597 Size: 416 53 83 399 24 646 1576 958 Cuts at: 4597 442 Size: 1753 Fragments arranged by size: 1753 1576 958 646 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5908 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4568 1239 Size: 3329 2579 Bpu10I CC'TnA_GC Cuts at: 2096 2520 2845 2096 Size: 424 325 5159 Fragments arranged by size: 5159 424 325 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2351 4033 4898 5196 2351 Size: 1682 865 298 3063 Fragments arranged by size: 3063 1682 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2477 2562 2969 4571 882 Size: 617 978 85 407 1602 2219 Fragments arranged by size: 2219 1602 978 617 407 85 BsaAI yAC'GTr Cuts at: 493 1204 3699 493 Size: 711 2495 2702 Fragments arranged by size: 2702 2495 711 BsaBI GATnn'nnATC Cuts at: 5902 5902 Size: 5908 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2025 2081 2313 2374 Size: 53 83 186 936 492 56 232 61 Cuts at: 2374 3715 4097 4732 275 Size: 1341 382 635 1451 Fragments arranged by size: 1451 1341 936 635 492 382 232 186 83 61 56 53 BsaWI w'CCGG_w Cuts at: 2128 4341 4739 5013 5160 5571 2128 Size: 2213 398 274 147 411 2465 Fragments arranged by size: 2465 2213 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5683 Fragments arranged by size: 5683 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2414 2537 1186 Size: 1228 123 4557 Fragments arranged by size: 4557 1228 123 BseYI C'CCAG_C Cuts at: 2084 2430 2605 2771 5111 5607 2084 Size: 346 175 166 2340 496 2385 Fragments arranged by size: 2385 2340 496 346 175 166 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2411 2642 1163 Size: 573 675 231 4429 Fragments arranged by size: 4429 675 573 231 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2139 2204 3358 4119 5147 1540 Size: 275 186 138 65 1154 761 1028 2301 Fragments arranged by size: 2301 1154 1028 761 275 186 138 65 BsiHKAI G_wGCw'C Cuts at: 2459 2679 2991 3969 4054 5125 2459 Size: 220 312 978 85 1071 3242 Fragments arranged by size: 3242 1071 978 312 220 85 BsmI GAATG_Cn' Cuts at: 3419 3512 3419 Size: 93 5815 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2284 2356 2459 2529 2679 2991 3227 Size: 399 494 72 103 70 150 312 236 Cuts at: 3227 3969 4054 5125 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 494 399 312 236 150 103 85 72 70 BspEI T'CCGG_A Cuts at: 5571 5571 Size: 5908 BspHI T'CATG_A Cuts at: 3797 5527 3797 Size: 1730 4178 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2478 3263 844 Size: 1634 785 3489 Fragments arranged by size: 3489 1634 785 BsrBI CCG'CTC Cuts at: 1394 1823 2053 2716 3795 1394 Size: 429 230 663 1079 3507 Fragments arranged by size: 3507 1079 663 429 230 BsrDI GCAATG_nn' Cuts at: 65 1248 2425 2646 2949 4403 4577 65 Size: 1183 1177 221 303 1454 174 1396 Fragments arranged by size: 1454 1396 1183 1177 303 221 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2101 3153 4552 4739 1700 Size: 110 291 1052 1399 187 2869 Fragments arranged by size: 2869 1399 1052 291 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5908 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5908 BssSI C'ACGA_G Cuts at: 3968 4980 3968 Size: 1012 4896 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5908 BstBI TT'CG_AA Cuts at: 4712 4712 Size: 5908 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5908 BstYI r'GATC_y Cuts at: 1972 2125 2301 2505 3238 3605 3990 4007 5459 Size: 153 176 204 733 367 385 17 1452 Cuts at: 5459 5545 5557 5903 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 733 385 367 346 204 176 153 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2178 2178 Size: 5908 BtgI C'CryG_G Cuts at: 513 1065 2004 2075 2121 513 Size: 552 939 71 46 4300 Fragments arranged by size: 4300 939 552 71 46 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5169 Fragments arranged by size: 5169 683 56 BtsI GCAGTG_nn' Cuts at: 2268 3424 4218 4238 2268 Size: 1156 794 20 3938 Fragments arranged by size: 3938 1156 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5908 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5873 DraI TTT'AAA Cuts at: 3212 3212 Size: 5908 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3097 4915 809 Size: 507 1781 1818 1802 Fragments arranged by size: 1818 1802 1781 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2136 2201 2265 Size: 54 1425 50 275 18 306 65 64 Cuts at: 2265 2742 3355 4244 5873 8 Size: 477 613 889 1629 43 Fragments arranged by size: 1629 1425 889 613 477 306 275 65 64 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2136 2201 3355 1537 Size: 275 324 65 1154 4090 Fragments arranged by size: 4090 1154 324 275 65 EarI CTCTTCn'nnn_ Cuts at: 1353 3838 1353 Size: 2485 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2237 2924 4495 4869 5015 387 Size: 1064 138 648 687 1571 374 146 1280 Fragments arranged by size: 1571 1280 1064 687 648 374 146 138 EcoICRI GAG'CTC Cuts at: 2457 2989 2457 Size: 532 5376 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2708 2898 3971 4568 5355 5623 1239 Size: 393 1076 190 1073 597 787 268 1524 Fragments arranged by size: 1524 1076 1073 787 597 393 268 190 EcoO109I rG'GnC_Cy Cuts at: 2143 2280 2353 2683 2997 2143 Size: 137 73 330 314 5054 Fragments arranged by size: 5054 330 314 137 73 EcoRI G'AATT_C Cuts at: 2345 3217 3595 2345 Size: 872 378 4658 Fragments arranged by size: 4658 872 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2976 3136 2976 Size: 160 5748 FspI TGC'GCA Cuts at: 4414 4414 Size: 5908 HaeII r_GCGC'y Cuts at: 1678 2028 2084 2339 2377 2646 3044 5055 1678 Size: 350 56 255 38 269 398 2011 2531 Fragments arranged by size: 2531 2011 398 350 269 255 56 38 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2490 2522 4549 4581 4623 Size: 32 135 32 918 32 2027 32 42 Cuts at: 4623 4655 1373 Size: 32 2626 Fragments arranged by size: 2626 2027 918 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3252 3498 669 Size: 988 281 1314 246 3079 Fragments arranged by size: 3079 1314 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5908 HpaI GTT'AAC Cuts at: 3498 3498 Size: 5908 KasI G'GCGC_C Cuts at: 2024 2080 2373 2024 Size: 56 293 5559 Fragments arranged by size: 5559 293 56 KpnI G_GTAC'C Cuts at: 3233 3233 Size: 5908 MfeI C'AATT_G Cuts at: 3507 3507 Size: 5908 MluI A'CGCG_T Cuts at: 5784 5784 Size: 5908 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5022 5206 1305 Size: 3717 184 2007 Fragments arranged by size: 3717 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4086 Fragments arranged by size: 4086 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3866 4225 4384 518 Size: 648 2700 359 159 2042 Fragments arranged by size: 2700 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2077 2277 2307 2635 3324 4001 5149 5394 Size: 471 200 30 328 689 677 1148 245 Cuts at: 5394 1606 Size: 2120 Fragments arranged by size: 2120 1148 689 677 471 328 245 200 30 NaeI GCC'GGC Cuts at: 2103 2103 Size: 5908 NarI GG'CG_CC Cuts at: 2025 2081 2374 2025 Size: 56 293 5559 Fragments arranged by size: 5559 293 56 NcoI C'CATG_G Cuts at: 513 1065 2004 2121 513 Size: 552 939 117 4300 Fragments arranged by size: 4300 939 552 117 NdeI CA'TA_TG Cuts at: 387 387 Size: 5908 NgoMIV G'CCGG_C Cuts at: 2101 2101 Size: 5908 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5908 NotI GC'GGCC_GC Cuts at: 2201 3355 2201 Size: 1154 4754 NspI r_CATG'y Cuts at: 1132 1229 3201 3266 4811 1132 Size: 97 1972 65 1545 2229 Fragments arranged by size: 2229 1972 1545 97 65 PasI CC'CwG_GG Cuts at: 1348 2923 1348 Size: 1575 4333 PciI A'CATG_T Cuts at: 3197 4807 3197 Size: 1610 4298 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5908 PfoI T'CCnGG_A Cuts at: 2591 2591 Size: 5908 PmeI GTTT'AAAC Cuts at: 3212 3212 Size: 5908 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3977 4009 5490 5522 3977 Size: 32 1481 32 4363 Fragments arranged by size: 4363 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2683 2997 2683 Size: 314 5594 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5908 PsiI TTA'TAA Cuts at: 3478 3478 Size: 5908 PspOMI G'GGCC_C Cuts at: 2280 2352 2280 Size: 72 5836 PstI C_TGCA'G Cuts at: 830 2415 3260 830 Size: 1585 845 3478 Fragments arranged by size: 3478 1585 845 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5908 PvuII CAG'CTG Cuts at: 2635 2635 Size: 5908 SacI G_AGCT'C Cuts at: 2459 2991 2459 Size: 532 5376 SacII CC_GC'GG Cuts at: 2078 2078 Size: 5908 SalI G'TCGA_C Cuts at: 1936 3250 1936 Size: 1314 4594 SbfI CC_TGCA'GG Cuts at: 3260 3260 Size: 5908 ScaI AGT'ACT Cuts at: 2834 4156 5868 2834 Size: 1322 1712 2874 Fragments arranged by size: 2874 1712 1322 SexAI A'CCwGG_T Cuts at: 3189 3189 Size: 5908 SfcI C'TryA_G Cuts at: 826 1045 2411 2630 3256 4391 5072 5263 826 Size: 219 1366 219 626 1135 681 191 1471 Fragments arranged by size: 1471 1366 1135 681 626 219 219 191 SfoI GGC'GCC Cuts at: 2026 2082 2375 2026 Size: 56 293 5559 Fragments arranged by size: 5559 293 56 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5908 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5908 SmaI CCC'GGG Cuts at: 1872 2117 2148 2285 3235 1872 Size: 245 31 137 950 4545 Fragments arranged by size: 4545 950 245 137 31 SmlI C'TyrA_G Cuts at: 820 1017 1943 2366 4012 4913 5175 820 Size: 197 926 423 1646 901 262 1553 Fragments arranged by size: 1646 1553 926 901 423 262 197 SnaBI TAC'GTA Cuts at: 493 3699 493 Size: 3206 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5908 SphI G_CATG'C Cuts at: 1132 3266 1132 Size: 2134 3774 SrfI GCCC'GGGC Cuts at: 2117 2117 Size: 5908 SspI AAT'ATT Cuts at: 5 52 3832 5 Size: 47 3780 2081 Fragments arranged by size: 3780 2081 47 StuI AGG'CCT Cuts at: 1517 2156 1517 Size: 639 5269 StyI C'CwwG_G Cuts at: 513 1065 2004 2121 2904 3347 513 Size: 552 939 117 783 443 3074 Fragments arranged by size: 3074 939 783 552 443 117 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3935 3952 4105 4290 4735 325 Size: 1255 2355 17 153 185 445 1498 Fragments arranged by size: 2355 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2832 4154 5866 Size: 274 80 33 51 1443 855 1322 1712 Cuts at: 5866 96 Size: 138 Fragments arranged by size: 1712 1443 1322 855 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5868 5900 5868 Size: 32 5876 Tth111I GACn'n_nGTC Cuts at: 1316 3191 1316 Size: 1875 4033 XbaI T'CTAG_A Cuts at: 3244 3244 Size: 5908 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2128 2737 2128 Size: 609 5299 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5908 XmaI C'CCGG_G Cuts at: 1870 2115 2146 2283 3233 1870 Size: 245 31 137 950 4545 Fragments arranged by size: 4545 950 245 137 31 XmnI GAAnn'nnTTC Cuts at: 4037 4037 Size: 5908 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3716 276 Size: 53 83 186 936 2182 2468 Fragments arranged by size: 2468 2182 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoNI EcoRV FseI FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII SanDI SapI SfiI SwaI