Restriction map for
pFN21AE0664
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# NO CUT ENZYMES ( 139 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaLI
ApoI AscI AseI AsnI Asp718I
AspEI AspI AvaII AviII AvrII
BalI BamHI BbrPI BbsI BbvCI
BciVI BclI BfrI BglII BlnI
BlpI Bpu1102I BpuAI BsaAI BsaBI
BsaI BsaWI BseAI BsgI BsiWI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstYI BstZ17I Bsu36I CelII ClaI
CpoI DraI DrdI Eam1105I EarI
Eco47III Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HincII HindII HindIII HpaI KpnI
Ksp632I MaeII MamI MboII MfeI
MflI MluI MluNI MroI MscI
MseI MunI NdeI NheI NsiI
NspI NspV PacI PflFI PinAI
PleI PmaCI PmeI PmlI PpuMI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacI SalI SapI SbfI
ScaI SexAI SfiI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Tru9I Tsp509I
Tth111I XbaI XcmI XhoII
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# ONE CUT ENZYMES ( 33 enzymes) #
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Asp700I 24 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 109 GTGCTC G[AT]GC[AT]^C
BbeI 871 GGCGCC GGCGC^C
BcgI 314 CGAGGTTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 1135 CTAG C^TAG
BsiHKAI 109 GTGCTC G[AT]GC[AT]^C
BsmI 527 GAATGC GAATGC(1/-1)
BspMI 413 ACCTGC ACCTGC(4/8)
BsrI 737 CCAGT ACTGG(1/-1)
BstXI 233 CCATCAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraIII 1021 CACCGGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 963 CGGCCG C^GGCCG
EclXI 963 CGGCCG C^GGCCG
Eco52I 963 CGGCCG C^GGCCG
HaeII 871 GGCGCC [GA]GCGC^[CT]
HgaI 181 GACGC GACGC(5/10)
HinfI 1087 GATTC G^A[ACGT]TC
KasI 871 GGCGCC G^GCGCC
MaeI 1135 CTAG C^TAG
NaeI 275 GCCGGC GCC^GGC
NarI 871 GGCGCC GG^CGCC
NcoI 1058 CCATGG C^CATGG
NgoMIV 275 GCCGGC G^CCGGC
NotI 962 GCGGCCGC GC^GGCCGC
NruI 795 TCGCGA TCG^CGA
PflMI 1036 CCAGATCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 748 GACGACTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 1107 CTGCAG CTGCA^G
SfoI 871 GGCGCC GGC^GCC
TfiI 1087 GATTC G^A[AT]TC
TspRI 738 CAGTG CA[GC]TG[ACGT][ACGT]^
Van91I 1036 CCAGATCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 24 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 17 enzymes) #
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AcyI 181 GACGCC G[GA]^CG[CT]C
871 GGCGCC
AfaI 711 GTAC GT^AC
930 GTAC
AlwI 12 GGATC GGATC(4/5)
813 GGATC
BanI 871 GGCGCC G^G[CT][GA]CC
1113 GGTGCC
BglI 275 GCCGGCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1097 GCCGGTCAGGC
BpmI 423 CTCCAG CTGGAG(16/14)
735 CTCCAG
BsaHI 181 GACGCC G[GA]^CG[CT]C
871 GGCGCC
DdeI 570 CTCAG C^T[ACGT]AG
820 CTGAG
EcoT14I 185 CCAAGG C^C[AT][AT]GG
1058 CCATGG
Hin1I 181 GACGCC G[GA]^CG[CT]C
871 GGCGCC
HphI 161 TCACC GGTGA(8/7)
943 TCACC
MslI 234 CATCAAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
733 CACTCCAGTG
PaeR7I 660 CTCGAG C^TCGAG
1008 CTCGAG
RsaI 711 GTAC GT^AC
930 GTAC
StyI 185 CCAAGG C^C[AT][AT]GG
1058 CCATGG
Tsp45I 160 GTCAC ^GT[GC]AC
803 GTGAC
XhoI 660 CTCGAG C^TCGAG
1008 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AccII AciI AluI ApaI AvaI
BanII BbvI BcnI BmyI BsaJI
BseRI BsiEI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I BsrFI
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr10I Cfr13I DpnII DraII
DsaI EaeI EcoO109I EcoRII FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HpaII ItaI KspI MaeIII
MboI MnlI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV SacII Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI SmlI TaqI
TseI TthHB8I XmaCI XmaI