(Circular) (Six-base) MAPSORT of: pFN21AE0664.seq Check: 2121 from: 1 to: 5881 pFN21AE0664 5881 bp With 182 enzymes: SgfI * April 15, 2010 09:33 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3691 278 Size: 53 83 186 936 2155 2468 Fragments arranged by size: 2468 2155 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3224 1937 Size: 1287 4594 Acc65I G'GTAC_C Cuts at: 3202 3202 Size: 5881 AclI AA'CG_TT Cuts at: 1563 4008 4381 1563 Size: 2445 373 3063 Fragments arranged by size: 3063 2445 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3944 5328 5596 1632 Size: 2312 1384 268 1917 Fragments arranged by size: 2312 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5684 AflIII A'CryG_T Cuts at: 1203 4780 5757 1203 Size: 3577 977 1327 Fragments arranged by size: 3577 1327 977 AgeI A'CCGG_T Cuts at: 4712 4712 Size: 5881 AhdI GACnn_n'nnGTC Cuts at: 4610 4610 Size: 5881 AleI CACnn'nnGTG Cuts at: 1166 2742 1166 Size: 1576 4305 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5881 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2204 2236 2204 Size: 32 5849 AlwNI CAG_nnn'CTG Cuts at: 3624 5196 3624 Size: 1572 4309 ApaI G_GGCC'C Cuts at: 2291 2385 2437 2510 2548 2630 2291 Size: 94 52 73 38 82 5542 Fragments arranged by size: 5542 94 82 73 52 38 ApaLI G'TGCA_C Cuts at: 3938 5094 3938 Size: 1156 4725 ApoI r'AATT_y Cuts at: 1493 3190 3416 3568 5677 1493 Size: 1697 226 152 2109 1697 Fragments arranged by size: 2109 1697 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4435 160 Size: 872 3403 1606 Fragments arranged by size: 3403 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5881 AvaI C'yCGr_G Cuts at: 1870 1943 2023 2317 2373 2665 2688 3013 3206 Size: 73 80 294 56 292 23 325 193 Cuts at: 3206 1870 Size: 4545 Fragments arranged by size: 4545 325 294 292 193 80 73 56 23 BamHI G'GATC_C Cuts at: 3211 3578 3211 Size: 367 5514 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2876 3118 3202 4657 618 Size: 325 217 627 1089 242 84 1455 1842 Fragments arranged by size: 1842 1455 1089 627 325 242 217 84 BanII G_rGCy'C Cuts at: 1391 2291 2385 2437 2510 2548 2630 2850 3054 Size: 900 94 52 73 38 82 220 204 Cuts at: 3054 3200 1391 Size: 146 4072 Fragments arranged by size: 4072 900 220 204 146 94 82 73 52 38 BbeI G_GCGC'C Cuts at: 2880 2880 Size: 5881 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4910 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2308 2342 4072 4106 1903 Size: 34 371 34 1730 34 3678 Fragments arranged by size: 3678 1730 371 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3773 4983 3773 Size: 1210 4671 BclI T'GATC_A Cuts at: 1548 1548 Size: 5881 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2286 3108 4492 Size: 107 122 71 631 605 614 822 1384 Cuts at: 4492 136 Size: 1525 Fragments arranged by size: 1525 1384 822 631 614 605 122 107 71 BglII A'GATC_T Cuts at: 5876 5876 Size: 5881 BlpI GC'TnA_GC Cuts at: 3298 3298 Size: 5881 Bme1580I G_kGCm'C Cuts at: 1790 2291 2385 2437 2510 2548 2630 3123 3942 Size: 501 94 52 73 38 82 493 819 Cuts at: 3942 5098 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 819 501 493 94 82 73 52 38 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5881 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3612 4570 442 Size: 416 53 83 399 24 2195 958 1753 Fragments arranged by size: 2195 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5881 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2662 2662 Size: 5881 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2413 2725 4541 1239 Size: 1174 312 1816 2579 Fragments arranged by size: 2579 1816 1174 312 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2785 4006 4871 5169 2785 Size: 1221 865 298 3497 Fragments arranged by size: 3497 1221 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4544 882 Size: 617 3045 2219 Fragments arranged by size: 3045 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3672 493 Size: 711 2468 2702 Fragments arranged by size: 2702 2468 711 BsaBI GATnn'nnATC Cuts at: 5875 5875 Size: 5881 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2187 2877 3688 4070 Size: 53 83 186 936 654 690 811 382 Cuts at: 4070 4705 275 Size: 635 1451 Fragments arranged by size: 1451 936 811 690 654 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4314 4712 4986 5133 5544 4314 Size: 398 274 147 411 4651 Fragments arranged by size: 4651 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2723 2753 1178 Size: 30 165 30 1320 30 4306 Fragments arranged by size: 4306 1320 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2928 2931 2945 3174 3177 1186 Size: 1742 3 14 229 3 3890 Fragments arranged by size: 3890 1742 229 14 3 3 BseYI C'CCAG_C Cuts at: 3084 5084 5580 3084 Size: 2000 496 3385 Fragments arranged by size: 3385 2000 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5308 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2314 2782 2806 2971 3007 3331 Size: 275 186 313 468 24 165 36 324 Cuts at: 3331 4092 5120 1540 Size: 761 1028 2301 Fragments arranged by size: 2301 1028 761 468 324 313 275 186 165 36 24 BsiHKAI G_wGCw'C Cuts at: 2118 3942 4027 5098 2118 Size: 1824 85 1071 2901 Fragments arranged by size: 2901 1824 1071 85 BsmI GAATG_Cn' Cuts at: 2538 3392 3485 2538 Size: 854 93 4934 Fragments arranged by size: 4934 854 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2559 2649 2778 2559 Size: 90 129 5662 Fragments arranged by size: 5662 129 90 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2118 2291 2385 2437 2510 2548 2630 Size: 399 328 173 94 52 73 38 82 Cuts at: 2630 2850 3054 3123 3200 3942 4027 5098 1391 Size: 220 204 69 77 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 328 220 204 173 94 85 82 77 73 69 52 38 BspEI T'CCGG_A Cuts at: 5544 5544 Size: 5881 BspHI T'CATG_A Cuts at: 3770 5500 3770 Size: 1730 4151 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2427 3236 844 Size: 1583 809 3489 Fragments arranged by size: 3489 1583 809 BsrBI CCG'CTC Cuts at: 1394 1823 3768 1394 Size: 429 1945 3507 Fragments arranged by size: 3507 1945 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4376 4550 65 Size: 1183 3128 174 1396 Fragments arranged by size: 3128 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2263 2280 3102 4525 4712 1700 Size: 110 453 17 822 1423 187 2869 Fragments arranged by size: 2869 1423 822 453 187 110 17 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5881 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5881 BssSI C'ACGA_G Cuts at: 3941 4953 3941 Size: 1012 4869 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5881 BstBI TT'CG_AA Cuts at: 4685 4685 Size: 5881 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5881 BstXI CCAn_nnnn'nTGG Cuts at: 2245 2245 Size: 5881 BstYI r'GATC_y Cuts at: 1972 3211 3578 3963 3980 5432 5518 5530 5876 Size: 1239 367 385 17 1452 86 12 346 Cuts at: 5876 1972 Size: 1977 Fragments arranged by size: 1977 1452 1239 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2308 2338 2357 2630 3063 513 Size: 552 939 304 30 19 273 433 3331 Fragments arranged by size: 3331 939 552 433 304 273 30 19 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5142 Fragments arranged by size: 5142 683 56 BtsI GCAGTG_nn' Cuts at: 3397 4191 4211 3397 Size: 794 20 5067 Fragments arranged by size: 5067 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5881 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5846 DraI TTT'AAA Cuts at: 3185 3185 Size: 5881 DraIII CAC_nnn'GTG Cuts at: 3031 3031 Size: 5881 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4888 809 Size: 507 3572 1802 Fragments arranged by size: 3572 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2098 2550 2734 Size: 54 1425 50 275 18 268 452 184 Cuts at: 2734 2968 2995 3328 4217 5846 8 Size: 234 27 333 889 1629 43 Fragments arranged by size: 1629 1425 889 452 333 275 268 234 184 54 50 43 27 18 EagI C'GGCC_G Cuts at: 1537 1812 2968 3328 1537 Size: 275 1156 360 4090 Fragments arranged by size: 4090 1156 360 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3811 1353 Size: 2458 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2423 3144 4468 4842 4988 387 Size: 1064 138 834 721 1324 374 146 1280 Fragments arranged by size: 1324 1280 1064 834 721 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2413 2725 3944 4541 5328 5596 1239 Size: 393 781 312 1219 597 787 268 1524 Fragments arranged by size: 1524 1219 787 781 597 393 312 268 EcoO109I rG'GnC_Cy Cuts at: 2044 2287 2433 2434 2544 2545 2044 Size: 243 146 1 110 1 5380 Fragments arranged by size: 5380 243 146 110 1 1 EcoRI G'AATT_C Cuts at: 3190 3568 3190 Size: 378 5503 FspI TGC'GCA Cuts at: 4387 4387 Size: 5881 HaeII r_GCGC'y Cuts at: 1678 2880 5028 1678 Size: 1202 2148 2531 Fragments arranged by size: 2531 2148 1202 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2558 2590 2648 2680 2996 Size: 32 135 32 986 32 58 32 316 Cuts at: 2996 3028 4522 4554 4596 4628 1373 Size: 32 1494 32 42 32 2626 Fragments arranged by size: 2626 1494 986 316 135 58 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3225 3471 669 Size: 988 281 1287 246 3079 Fragments arranged by size: 3079 1287 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5881 HpaI GTT'AAC Cuts at: 3471 3471 Size: 5881 KasI G'GCGC_C Cuts at: 2876 2876 Size: 5881 KpnI G_GTAC'C Cuts at: 3206 3206 Size: 5881 MfeI C'AATT_G Cuts at: 3480 3480 Size: 5881 MluI A'CGCG_T Cuts at: 5757 5757 Size: 5881 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4995 5179 1305 Size: 3690 184 2007 Fragments arranged by size: 3690 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4059 Fragments arranged by size: 4059 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2243 2742 3839 4198 4357 518 Size: 648 1077 499 1097 359 159 2042 Fragments arranged by size: 2042 1097 1077 648 499 359 159 MspA1I CmG'CkG Cuts at: 1606 2310 2340 2359 2523 2599 2632 2964 3297 Size: 704 30 19 164 76 33 332 333 Cuts at: 3297 3974 5122 5367 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 704 677 333 332 245 164 76 33 30 19 NaeI GCC'GGC Cuts at: 2282 2282 Size: 5881 NarI GG'CG_CC Cuts at: 2877 2877 Size: 5881 NcoI C'CATG_G Cuts at: 513 1065 2004 3063 513 Size: 552 939 1059 3331 Fragments arranged by size: 3331 1059 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5881 NgoMIV G'CCGG_C Cuts at: 2280 2280 Size: 5881 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5881 NotI GC'GGCC_GC Cuts at: 2968 3328 2968 Size: 360 5521 NruI TCG'CGA Cuts at: 2802 2802 Size: 5881 NspI r_CATG'y Cuts at: 1132 1229 3239 4784 1132 Size: 97 2010 1545 2229 Fragments arranged by size: 2229 2010 1545 97 PasI CC'CwG_GG Cuts at: 1348 3123 1348 Size: 1775 4106 PciI A'CATG_T Cuts at: 4780 4780 Size: 5881 PflMI CCAn_nnn'nTGG Cuts at: 1108 3047 1108 Size: 1939 3942 PfoI T'CCnGG_A Cuts at: 2748 2748 Size: 5881 PmeI GTTT'AAAC Cuts at: 3185 3185 Size: 5881 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3950 3982 5463 5495 3950 Size: 32 1481 32 4336 Fragments arranged by size: 4336 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2757 1613 Size: 1144 4737 PsiI TTA'TAA Cuts at: 3451 3451 Size: 5881 PspOMI G'GGCC_C Cuts at: 2287 2381 2433 2506 2544 2626 2287 Size: 94 52 73 38 82 5542 Fragments arranged by size: 5542 94 82 73 52 38 PspXI vC'TCGA_Gb Cuts at: 2665 3013 2665 Size: 348 5533 PstI C_TGCA'G Cuts at: 830 3116 3233 830 Size: 2286 117 3478 Fragments arranged by size: 3478 2286 117 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5881 SacII CC_GC'GG Cuts at: 2311 2341 2360 2633 2311 Size: 30 19 273 5559 Fragments arranged by size: 5559 273 30 19 SalI G'TCGA_C Cuts at: 1936 3223 1936 Size: 1287 4594 SbfI CC_TGCA'GG Cuts at: 3233 3233 Size: 5881 ScaI AGT'ACT Cuts at: 4129 5841 4129 Size: 1712 4169 SfcI C'TryA_G Cuts at: 826 1045 2773 2959 3112 3229 4364 5045 5236 Size: 219 1728 186 153 117 1135 681 191 Cuts at: 5236 826 Size: 1471 Fragments arranged by size: 1728 1471 1135 681 219 191 186 153 117 SfoI GGC'GCC Cuts at: 2878 2878 Size: 5881 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5881 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5881 SmaI CCC'GGG Cuts at: 1872 2025 2375 2690 3208 1872 Size: 153 350 315 518 4545 Fragments arranged by size: 4545 518 350 315 153 SmlI C'TyrA_G Cuts at: 820 1017 1943 2665 2764 3013 3985 4886 5148 Size: 197 926 722 99 249 972 901 262 Cuts at: 5148 820 Size: 1553 Fragments arranged by size: 1553 972 926 901 722 262 249 197 99 SnaBI TAC'GTA Cuts at: 493 3672 493 Size: 3179 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5881 SphI G_CATG'C Cuts at: 1132 3239 1132 Size: 2107 3774 SspI AAT'ATT Cuts at: 5 52 3805 5 Size: 47 3753 2081 Fragments arranged by size: 3753 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5881 StyI C'CwwG_G Cuts at: 513 1065 2004 2190 3063 3320 513 Size: 552 939 186 873 257 3074 Fragments arranged by size: 3074 939 873 552 257 186 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2145 2735 2796 3908 3925 4078 4263 Size: 1255 565 590 61 1112 17 153 185 Cuts at: 4263 4708 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1112 590 565 445 185 153 61 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2715 4127 5839 Size: 274 80 33 51 1443 738 1412 1712 Cuts at: 5839 96 Size: 138 Fragments arranged by size: 1712 1443 1412 738 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5841 5873 5841 Size: 32 5849 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5881 XbaI T'CTAG_A Cuts at: 3217 3217 Size: 5881 XhoI C'TCGA_G Cuts at: 1943 2665 3013 1943 Size: 722 348 4811 Fragments arranged by size: 4811 722 348 XmaI C'CCGG_G Cuts at: 1870 2023 2373 2688 3206 1870 Size: 153 350 315 518 4545 Fragments arranged by size: 4545 518 350 315 153 XmnI GAAnn'nnTTC Cuts at: 2033 4010 2033 Size: 1977 3904 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3689 276 Size: 53 83 186 936 2155 2468 Fragments arranged by size: 2468 2155 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PspXI PstI PvuI SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI NsiI PacI PmlI PpuMI PsrI PvuII RsrII SacI SanDI SapI SexAI SfiI SrfI SwaI XcmI