Restriction map for
pFN21AE0664
(Linear) (Six-base) MAPSORT of: KIEE0664.seq Check: 9899 from: 1 to: 1176
KIEE0664.orf
With 182 enzymes: SgfI *
October 7, 2009 14:27 ..
AleI CACnn'nnGTG
Cuts at: 0 737 1176
Size: 737 439
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 199 231 1176
Size: 199 32 945
Fragments arranged by size:
945 199 32
ApaI G_GGCC'C
Cuts at: 0 286 380 432 505 543 625 1176
Size: 286 94 52 73 38 82 551
Fragments arranged by size:
551 286 94 82 73 52 38
AvaI C'yCGr_G
Cuts at: 0 18 312 368 660 683 1008 1176
Size: 18 294 56 292 23 325 168
Fragments arranged by size:
325 294 292 168 56 23 18
BanI G'GyrC_C
Cuts at: 0 871 1113 1176
Size: 871 242 63
Fragments arranged by size:
871 242 63
BanII G_rGCy'C
Cuts at: 0 286 380 432 505 543 625 845 1049
Size: 286 94 52 73 38 82 220 204
Cuts at: 1049 1176
Size: 127
Fragments arranged by size:
286 220 204 127 94 82 73 52
38
BbeI G_GCGC'C
Cuts at: 0 875 1176
Size: 875 301
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 303 337 1176
Size: 303 34 839
Fragments arranged by size:
839 303 34
BglI GCCn_nnn'nGGC
Cuts at: 0 281 1103 1176
Size: 281 822 73
Fragments arranged by size:
822 281 73
Bme1580I G_kGCm'C
Cuts at: 0 286 380 432 505 543 625 1118 1176
Size: 286 94 52 73 38 82 493 58
Fragments arranged by size:
493 286 94 82 73 58 52 38
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 657 1176
Size: 657 519
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 408 720 1176
Size: 408 312 456
Fragments arranged by size:
456 408 312
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 780 1176
Size: 780 396
BsaHI Gr'CG_yC
Cuts at: 0 182 872 1176
Size: 182 690 304
Fragments arranged by size:
690 304 182
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 718 748 1176
Size: 718 30 428
Fragments arranged by size:
718 428 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 923 926 940 1169 1172 1176
Size: 923 3 14 229 3 4
Fragments arranged by size:
923 229 14 4 3 3
BseYI C'CCAG_C
Cuts at: 0 1079 1176
Size: 1079 97
BsiEI CG_ry'CG
Cuts at: 0 309 777 801 966 1002 1176
Size: 309 468 24 165 36 174
Fragments arranged by size:
468 309 174 165 36 24
BsiHKAI G_wGCw'C
Cuts at: 0 113 1176
Size: 113 1063
BsmI GAATG_Cn'
Cuts at: 0 533 1176
Size: 533 643
BsmBI CGTCTCn'nnnn_
Cuts at: 0 554 644 773 1176
Size: 554 90 129 403
Fragments arranged by size:
554 403 129 90
Bsp1286I G_dGCh'C
Cuts at: 0 113 286 380 432 505 543 625 845
Size: 113 173 94 52 73 38 82 220
Cuts at: 845 1049 1118 1176
Size: 204 69 58
Fragments arranged by size:
220 204 173 113 94 82 73 69
58 52 38
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 422 1176
Size: 422 754
BsrFI r'CCGG_y
Cuts at: 0 258 275 1097 1176
Size: 258 17 822 79
Fragments arranged by size:
822 258 79 17
BstXI CCAn_nnnn'nTGG
Cuts at: 0 240 1176
Size: 240 936
BtgI C'CryG_G
Cuts at: 0 303 333 352 625 1058 1176
Size: 303 30 19 273 433 118
Fragments arranged by size:
433 303 273 118 30 19
DraIII CAC_nnn'GTG
Cuts at: 0 1026 1176
Size: 1026 150
EaeI y'GGCC_r
Cuts at: 0 93 545 729 963 990 1176
Size: 93 452 184 234 27 186
Fragments arranged by size:
452 234 186 184 93 27
EagI C'GGCC_G
Cuts at: 0 963 1176
Size: 963 213
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 418 1139 1176
Size: 418 721 37
Fragments arranged by size:
721 418 37
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 408 720 1176
Size: 408 312 456
Fragments arranged by size:
456 408 312
EcoO109I rG'GnC_Cy
Cuts at: 0 39 282 428 429 539 540 1176
Size: 39 243 146 1 110 1 636
Fragments arranged by size:
636 243 146 110 39 1 1
HaeII r_GCGC'y
Cuts at: 0 875 1176
Size: 875 301
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 553 585 643 675 991 1023 1176
Size: 553 32 58 32 316 32 153
Fragments arranged by size:
553 316 153 58 32 32 32
KasI G'GCGC_C
Cuts at: 0 871 1176
Size: 871 305
MslI CAynn'nnrTG
Cuts at: 0 238 737 1176
Size: 238 499 439
Fragments arranged by size:
499 439 238
MspA1I CmG'CkG
Cuts at: 0 305 335 354 518 594 627 959 1176
Size: 305 30 19 164 76 33 332 217
Fragments arranged by size:
332 305 217 164 76 33 30 19
NaeI GCC'GGC
Cuts at: 0 277 1176
Size: 277 899
NarI GG'CG_CC
Cuts at: 0 872 1176
Size: 872 304
NcoI C'CATG_G
Cuts at: 0 1058 1176
Size: 1058 118
NgoMIV G'CCGG_C
Cuts at: 0 275 1176
Size: 275 901
NotI GC'GGCC_GC
Cuts at: 0 963 1176
Size: 963 213
NruI TCG'CGA
Cuts at: 0 797 1176
Size: 797 379
PasI CC'CwG_GG
Cuts at: 0 1118 1176
Size: 1118 58
PflMI CCAn_nnn'nTGG
Cuts at: 0 1042 1176
Size: 1042 134
PfoI T'CCnGG_A
Cuts at: 0 743 1176
Size: 743 433
PshAI GACnn'nnGTC
Cuts at: 0 752 1176
Size: 752 424
PspOMI G'GGCC_C
Cuts at: 0 282 376 428 501 539 621 1176
Size: 282 94 52 73 38 82 555
Fragments arranged by size:
555 282 94 82 73 52 38
PspXI vC'TCGA_Gb
Cuts at: 0 660 1008 1176
Size: 660 348 168
Fragments arranged by size:
660 348 168
PstI C_TGCA'G
Cuts at: 0 1111 1176
Size: 1111 65
SacII CC_GC'GG
Cuts at: 0 306 336 355 628 1176
Size: 306 30 19 273 548
Fragments arranged by size:
548 306 273 30 19
SfcI C'TryA_G
Cuts at: 0 768 954 1107 1176
Size: 768 186 153 69
Fragments arranged by size:
768 186 153 69
SfoI GGC'GCC
Cuts at: 0 873 1176
Size: 873 303
SmaI CCC'GGG
Cuts at: 0 20 370 685 1176
Size: 20 350 315 491
Fragments arranged by size:
491 350 315 20
SmlI C'TyrA_G
Cuts at: 0 660 759 1008 1176
Size: 660 99 249 168
Fragments arranged by size:
660 249 168 99
StyI C'CwwG_G
Cuts at: 0 185 1058 1176
Size: 185 873 118
Fragments arranged by size:
873 185 118
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 140 730 791 1176
Size: 140 590 61 385
Fragments arranged by size:
590 385 140 61
TatI w'GTAC_w
Cuts at: 0 710 1176
Size: 710 466
XhoI C'TCGA_G
Cuts at: 0 660 1008 1176
Size: 660 348 168
Fragments arranged by size:
660 348 168
XmaI C'CCGG_G
Cuts at: 0 18 368 683 1176
Size: 18 350 315 493
Fragments arranged by size:
493 350 315 18
XmnI GAAnn'nnTTC
Cuts at: 0 28 1176
Size: 28 1148
Enzymes that do cut:
AleI AloI ApaI AvaI BanI BanII BbeI BcgI
BglI Bme1580I BplI BpmI BpuEI BsaHI BsaXI BseRI
BseYI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrFI
BstXI BtgI DraIII EaeI EagI EciI Eco57MI EcoO109I
HaeII Hin4I KasI MslI MspA1I NaeI NarI NcoI
NgoMIV NotI NruI PasI PflMI PfoI PshAI PspOMI
PspXI PstI SacII SfcI SfoI SmaI SmlI StyI
TaqII TatI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AlfI AlwNI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BbsI BbvCI BciVI
BclI BfrBI BglII BlpI BmgBI BmrI BmtI Bpu10I
BsaI BsaAI BsaBI BsaWI BsgI BsiWI BspEI BspHI
BsrBI BsrDI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI
DrdI EarI EcoICRI EcoNI EcoRI EcoRV FalI FseI
FspI FspAI HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI NdeI NheI NsiI NspI PacI PciI
PmeI PmlI PpiI PpuMI PsiI PsrI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SexAI
SfiI SgfI SgrAI SnaBI SpeI SphI SrfI SspI
StuI SwaI TstI Tth111I XbaI XcmI ZraI