Restriction map for
pFN21AE0621
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# NO CUT ENZYMES ( 178 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AfaI AflII
AflIII AgeI AhdI Alw44I AlwI
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AviII AvrII
BalI BamHI BanI BbeI BbrPI
BbsI BbvCI BcgI BcnI BfrI
BglI BglII BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BsgI BsiEI BsiHKAI
BsiWI BsmBI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstYI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraI DraII DraIII
DrdI DsaI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO109I
EcoO65I EcoRI EcoRV EcoT14I FauI
FseI FspI HapII HgaI Hin1I
HindIII HpaII HphI KasI KpnI
KspI MaeII MamI MfeI MflI
MluI MluNI MroI MscI MslI
MspA1I MspI MunI MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NspV PacI
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
StyI SwaI Tth111I Van91I XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AciI 460 GCGG CCGC(-3/-1)
AlwNI 488 CAGCGCCTG CAG[ACGT][ACGT][ACGT]^CTG
AvaII 96 GGACC G^G[AT]CC
BanII 54 GGGCTC G[GA]GC[CT]^C
BbvI 487 GCAGC GCAGC(8/12)
BciVI 480 GTATCC GTATCC(6/5)
BclI 333 TGATCA T^GATCA
BfaI 545 CTAG C^TAG
BsaJI 26 CCCTGG C^C[ACGT][ACGT]GG
BsiYI 45 CCTTTCAGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 45 CCTTTCAGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI 195 GGGAC GGGAC(10/14)
BsmI 33 GAATGC GAATGC(1/-1)
BsrI 258 CCAGT ACTGG(1/-1)
Cac8I 249 GCAAGC GC[ACGT]^[ACGT]GC
CfoI 490 GCGC GCG^C
DpnII 334 GATC ^GATC
EcoNI 45 CCTTTCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 35 ATGCAT ATGCA^T
FbaI 333 TGATCA T^GATCA
Fnu4HI 487 GCAGC GC^[ACGT]GC
HaeII 489 AGCGCC [GA]GCGC^[CT]
HaeIII 279 GGCC GG^CC
HhaI 490 GCGC GCG^C
HinP1I 490 GCGC G^CGC
HincII 220 GTTAAC GT[CT]^[GA]AC
HindII 220 GTTAAC GT[CT]^[GA]AC
HinfI 354 GATTC G^A[ACGT]TC
HpaI 220 GTTAAC GTT^AAC
ItaI 487 GCAGC GC^[ACGT]GC
MaeI 545 CTAG C^TAG
MaeIII 415 GTCAC ^GT[ACGT]AC
MboI 334 GATC ^GATC
NdeII 334 GATC ^GATC
NlaIV 96 GGACCC GG[ACGT]^[ACGT]CC
NsiI 35 ATGCAT ATGCA^T
NspI 312 ACATGC [GA]CATG^[CT]
PstI 61 CTGCAG CTGCA^G
Sau3AI 334 GATC ^GATC
SfcI 61 CTGCAG C^T[GA][CT]AG
TfiI 354 GATTC G^A[AT]TC
TseI 487 GCAGC G^C[AT]GC
Tsp45I 415 GTCAC ^GT[GC]AC
Tsp509I 105 AATT ^AATT
XbaI 544 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 25 enzymes) #
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AluI 128 AGCT AG^CT
293 AGCT
AvaI 203 CTCGAG C^[CT]CG[GA]G
370 CTCGAG
BmyI 54 GGGCTC G[AGT]GC[ACT]^C
308 GGGCAC
BpmI 133 CTCCAG CTGGAG(16/14)
28 CTGGAG
BseRI 121 CTCCTC GAGGAG(10/8)
130 CTCCTC
BsmAI 263 GTCTC GTCTC(1/5)
368 GTCTC
BsoBI 203 CTCGAG C^[CT]CG[GA]G
370 CTCGAG
Bsp1286I 54 GGGCTC G[AGT]GC[ACT]^C
308 GGGCAC
BssKI 27 CCTGG ^CC[ACGT]GG
93 CCTGG
BstNI 27 CCTGG CC^[AT]GG
93 CCTGG
BstXI 135 CCAGAAGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
287 CCAAGAAGCTGG
Cfr13I 96 GGACC G^G[ACGT]CC
279 GGCCC
EarI 20 CTCTTC CTCTTC(1/4)
169 CTCTTC
EcoRII 27 CCTGG ^CC[AT]GG
93 CCTGG
Ksp632I 20 CTCTTC CTCTTC(1/4)
169 CTCTTC
MseI 221 TTAA T^TAA
509 TTAA
MvaI 27 CCTGG CC^[AT]GG
93 CCTGG
NlaIII 313 CATG CATG^
554 CATG
PaeR7I 203 CTCGAG C^TCGAG
370 CTCGAG
Sau96I 96 GGACC G^G[ACGT]CC
279 GGCCC
ScrFI 27 CCTGG CC^[ACGT]GG
93 CCTGG
SfaNI 472 GATGC GCATC(5/9)
37 GCATC
Tru9I 221 TTAA T^TAA
509 TTAA
XcmI 258 CCAGTGTCTCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
287 CCAAGAAGCTGGTGG
XhoI 203 CTCGAG C^TCGAG
370 CTCGAG
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# 3 OR MORE CUT ENZYMES ( 10 enzymes) #
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BstF5I DdeI FokI MboII MnlI
MwoI SmlI TaqI TspRI TthHB8I