(Circular) (Six-base) MAPSORT of: pFN21AE0621.seq Check: 7118 from: 1 to: 5275 pFN21AE0621 5275 bp With 182 enzymes: SgfI * April 15, 2010 09:33 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3085 278 Size: 53 83 186 936 1549 2468 Fragments arranged by size: 2468 1549 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2618 1937 Size: 681 4594 Acc65I G'GTAC_C Cuts at: 2596 2596 Size: 5275 AclI AA'CG_TT Cuts at: 1563 3402 3775 1563 Size: 1839 373 3063 Fragments arranged by size: 3063 1839 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3338 4722 4990 1632 Size: 1706 1384 268 1917 Fragments arranged by size: 1917 1706 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5078 AflIII A'CryG_T Cuts at: 1203 4174 5151 1203 Size: 2971 977 1327 Fragments arranged by size: 2971 1327 977 AgeI A'CCGG_T Cuts at: 4106 4106 Size: 5275 AhdI GACnn_n'nnGTC Cuts at: 4004 4004 Size: 5275 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5275 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5275 AlwNI CAG_nnn'CTG Cuts at: 2498 3018 4590 2498 Size: 520 1572 3183 Fragments arranged by size: 3183 1572 520 ApaLI G'TGCA_C Cuts at: 3332 4488 3332 Size: 1156 4119 ApoI r'AATT_y Cuts at: 1493 2584 2810 2962 5071 1493 Size: 1091 226 152 2109 1697 Fragments arranged by size: 2109 1697 1091 226 152 AseI AT'TA_AT Cuts at: 160 1032 3829 160 Size: 872 2797 1606 Fragments arranged by size: 2797 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5275 AvaI C'yCGr_G Cuts at: 1870 1943 2208 2375 2600 1870 Size: 73 265 167 225 4545 Fragments arranged by size: 4545 265 225 167 73 BamHI G'GATC_C Cuts at: 2605 2972 2605 Size: 367 4908 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2596 4051 618 Size: 325 217 627 809 1455 1842 Fragments arranged by size: 1842 1455 809 627 325 217 BanII G_rGCy'C Cuts at: 1391 2063 2594 1391 Size: 672 531 4072 Fragments arranged by size: 4072 672 531 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4304 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3466 3500 1903 Size: 34 1529 34 3678 Fragments arranged by size: 3678 1529 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2496 3167 4377 2496 Size: 671 1210 3394 Fragments arranged by size: 3394 1210 671 BclI T'GATC_A Cuts at: 1548 2338 1548 Size: 790 4485 BfrBI ATG'CAT Cuts at: 2042 2042 Size: 5275 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3886 136 Size: 107 122 71 631 605 2214 1525 Fragments arranged by size: 2214 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5270 5270 Size: 5275 BlpI GC'TnA_GC Cuts at: 2692 2692 Size: 5275 Bme1580I G_kGCm'C Cuts at: 1790 2317 3336 4492 1790 Size: 527 1019 1156 2573 Fragments arranged by size: 2573 1156 1019 527 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5275 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3006 3964 442 Size: 416 53 83 399 24 1589 958 1753 Fragments arranged by size: 1753 1589 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5275 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2401 2401 Size: 5275 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2054 2123 3935 1239 Size: 815 69 1812 2579 Fragments arranged by size: 2579 1812 815 69 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2114 2307 2371 3400 4265 4563 2114 Size: 193 64 1029 865 298 2826 Fragments arranged by size: 2826 1029 865 298 193 64 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3938 882 Size: 617 2439 2219 Fragments arranged by size: 2439 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3066 493 Size: 711 1862 2702 Fragments arranged by size: 2702 1862 711 BsaBI GATnn'nnATC Cuts at: 5269 5269 Size: 5275 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3082 3464 4099 275 Size: 53 83 186 936 1549 382 635 1451 Fragments arranged by size: 1549 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3708 4106 4380 4527 4938 3708 Size: 398 274 147 411 4045 Fragments arranged by size: 4045 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5050 Fragments arranged by size: 5050 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2117 2126 1186 Size: 931 9 4335 Fragments arranged by size: 4335 931 9 BseYI C'CCAG_C Cuts at: 4478 4974 4478 Size: 496 4779 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4702 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2725 3486 4514 1540 Size: 275 186 724 761 1028 2301 Fragments arranged by size: 2301 1028 761 724 275 186 BsiHKAI G_wGCw'C Cuts at: 3336 3421 4492 3336 Size: 85 1071 4119 Fragments arranged by size: 4119 1071 85 BsmI GAATG_Cn' Cuts at: 2044 2786 2879 2044 Size: 742 93 4440 Fragments arranged by size: 4440 742 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2063 2317 2594 3336 3421 4492 1391 Size: 399 273 254 277 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 277 273 254 85 BspEI T'CCGG_A Cuts at: 4938 4938 Size: 5275 BspHI T'CATG_A Cuts at: 3164 4894 3164 Size: 1730 3545 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2630 844 Size: 1786 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3162 1394 Size: 429 1339 3507 Fragments arranged by size: 3507 1339 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3770 3944 65 Size: 1183 2522 174 1396 Fragments arranged by size: 2522 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3919 4106 1700 Size: 110 2109 187 2869 Fragments arranged by size: 2869 2109 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5275 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5275 BssSI C'ACGA_G Cuts at: 3335 4347 3335 Size: 1012 4263 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5275 BstBI TT'CG_AA Cuts at: 4079 4079 Size: 5275 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5275 BstXI CCAn_nnnn'nTGG Cuts at: 2147 2299 2147 Size: 152 5123 BstYI r'GATC_y Cuts at: 1972 2605 2972 3357 3374 4826 4912 4924 5270 Size: 633 367 385 17 1452 86 12 346 Cuts at: 5270 1972 Size: 1977 Fragments arranged by size: 1977 1452 633 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 3784 Fragments arranged by size: 3784 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2323 487 Size: 683 56 1097 3439 Fragments arranged by size: 3439 1097 683 56 BtsI GCAGTG_nn' Cuts at: 2421 2791 3585 3605 2421 Size: 370 794 20 4091 Fragments arranged by size: 4091 794 370 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5275 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5240 DraI TTT'AAA Cuts at: 2579 2579 Size: 5275 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4282 809 Size: 507 2966 1802 Fragments arranged by size: 2966 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2722 3611 5240 Size: 54 1425 50 275 18 892 889 1629 Cuts at: 5240 8 Size: 43 Fragments arranged by size: 1629 1425 892 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2722 1537 Size: 275 910 4090 Fragments arranged by size: 4090 910 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2031 2180 2566 3205 1353 Size: 678 149 386 639 3423 Fragments arranged by size: 3423 678 639 386 149 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2480 3862 4236 4382 387 Size: 1064 138 891 1382 374 146 1280 Fragments arranged by size: 1382 1280 1064 891 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2054 2123 3338 3935 4722 4990 1239 Size: 393 422 69 1215 597 787 268 1524 Fragments arranged by size: 1524 1215 787 597 422 393 268 69 EcoNI CCTnn'n_nnAGG Cuts at: 2054 2054 Size: 5275 EcoRI G'AATT_C Cuts at: 2584 2962 2584 Size: 378 4897 FspI TGC'GCA Cuts at: 3781 3781 Size: 5275 HaeII r_GCGC'y Cuts at: 1678 2498 4422 1678 Size: 820 1924 2531 Fragments arranged by size: 2531 1924 820 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2027 2059 2323 2355 3916 Size: 32 135 32 455 32 264 32 1561 Cuts at: 3916 3948 3990 4022 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1561 455 264 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2227 2619 2865 669 Size: 988 281 289 392 246 3079 Fragments arranged by size: 3079 988 392 289 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5275 HpaI GTT'AAC Cuts at: 2227 2865 2227 Size: 638 4637 KpnI G_GTAC'C Cuts at: 2600 2600 Size: 5275 MfeI C'AATT_G Cuts at: 2874 2874 Size: 5275 MluI A'CGCG_T Cuts at: 5151 5151 Size: 5275 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4389 4573 1305 Size: 3084 184 2007 Fragments arranged by size: 3084 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3453 Fragments arranged by size: 3453 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3233 3592 3751 518 Size: 648 2067 359 159 2042 Fragments arranged by size: 2067 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2691 3368 4516 4761 1606 Size: 1085 677 1148 245 2120 Fragments arranged by size: 2120 1148 1085 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3784 Fragments arranged by size: 3784 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5275 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5275 NotI GC'GGCC_GC Cuts at: 2722 2722 Size: 5275 NsiI A_TGCA'T Cuts at: 2044 2044 Size: 5275 NspI r_CATG'y Cuts at: 1132 1229 2321 2633 4178 1132 Size: 97 1092 312 1545 2229 Fragments arranged by size: 2229 1545 1092 312 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5275 PciI A'CATG_T Cuts at: 4174 4174 Size: 5275 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5275 PmeI GTTT'AAAC Cuts at: 2579 2579 Size: 5275 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3344 3376 4857 4889 3344 Size: 32 1481 32 3730 Fragments arranged by size: 3730 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5275 PsiI TTA'TAA Cuts at: 2845 2845 Size: 5275 PspXI vC'TCGA_Gb Cuts at: 2208 2208 Size: 5275 PstI C_TGCA'G Cuts at: 830 2070 2627 830 Size: 1240 557 3478 Fragments arranged by size: 3478 1240 557 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5275 SalI G'TCGA_C Cuts at: 1936 2617 1936 Size: 681 4594 SbfI CC_TGCA'GG Cuts at: 2627 2627 Size: 5275 ScaI AGT'ACT Cuts at: 3523 5235 3523 Size: 1712 3563 SfcI C'TryA_G Cuts at: 826 1045 2066 2623 3758 4439 4630 826 Size: 219 1021 557 1135 681 191 1471 Fragments arranged by size: 1471 1135 1021 681 557 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5275 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5275 SmaI CCC'GGG Cuts at: 1872 2602 1872 Size: 730 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2129 2208 2322 2375 2386 3379 Size: 197 926 186 79 114 53 11 993 Cuts at: 3379 4280 4542 820 Size: 901 262 1553 Fragments arranged by size: 1553 993 926 901 262 197 186 114 79 53 11 SnaBI TAC'GTA Cuts at: 493 3066 493 Size: 2573 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5275 SphI G_CATG'C Cuts at: 1132 2633 1132 Size: 1501 3774 SspI AAT'ATT Cuts at: 5 52 3199 5 Size: 47 3147 2081 Fragments arranged by size: 3147 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5275 StyI C'CwwG_G Cuts at: 513 1065 2004 2714 513 Size: 552 939 710 3074 Fragments arranged by size: 3074 939 710 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3302 3319 3472 3657 4102 325 Size: 1255 1722 17 153 185 445 1498 Fragments arranged by size: 1722 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3521 5233 96 Size: 274 80 33 51 1443 1544 1712 138 Fragments arranged by size: 1712 1544 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5235 5267 5235 Size: 32 5243 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5275 XbaI T'CTAG_A Cuts at: 2549 2611 2549 Size: 62 5213 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2270 2299 2270 Size: 29 5246 XhoI C'TCGA_G Cuts at: 1943 2208 2375 1943 Size: 265 167 4843 Fragments arranged by size: 4843 265 167 XmaI C'CCGG_G Cuts at: 1870 2600 1870 Size: 730 4545 XmnI GAAnn'nnTTC Cuts at: 3404 3404 Size: 5275 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3083 276 Size: 53 83 186 936 1549 2468 Fragments arranged by size: 2468 1549 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspXI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI