Restriction map for
pFN21AB0936
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# NO CUT ENZYMES ( 163 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AviII
AvrII BalI BamHI BanI BbeI
BbrPI BbsI BbvCI BbvI BcgI
BciVI BclI BcnI BfrI BglI
BglII BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BsaI BseAI
BsgI BsiEI BsiWI BsmBI BsoBI
BspDI BspEI BspHI BspLU11I BsrBI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstYI BstZ17I
Bsu36I CelII CfoI ClaI CpoI
DraIII EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoT22I FauI FbaI FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HincII HindII HindIII HpaI
KasI KpnI KspI MaeII MamI
MflI MluI MluNI MroI MscI
MspA1I MvnI NaeI NarI NciI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsrII SacII SalI SbfI
ScaI SexAI SfcI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspI StuI SwaI
TseI Tth111I XbaI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 34 enzymes) #
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AspHI 406 GAGCTC G[AT]GC[AT]^C
BpmI 958 CTGGAG CTGGAG(16/14)
BsaWI 37 ACCGGA [AT]^CCGG[AT]
BsiHKAI 406 GAGCTC G[AT]GC[AT]^C
BsmAI 1042 GAGAC GTCTC(1/5)
BsmI 878 GCATTC GAATGC(1/-1)
Bsp1407I 1031 TGTACA T^GTACA
BspMI 729 GCAGGT ACCTGC(4/8)
BsrDI 239 CATTGC GCAATG(2/0)
BsrFI 387 GCCGGT [GA]^CCGG[CT]
BsrGI 1031 TGTACA T^GTACA
BstXI 131 CCAATAATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 387 GCCGGT [GA]^CCGG[CT]
DraI 111 TTTAAA TTT^AAA
DrdI 85 GACCTAATAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 935 CCATGG C^C[GA][CT]GG
EcoRV 1111 GATATC GAT^ATC
EcoT14I 935 CCATGG C^C[AT][AT]GG
Fnu4HI 1088 GCCGC GC^[ACGT]GC
HaeIII 615 GGCC GG^CC
ItaI 1088 GCCGC GC^[ACGT]GC
MfeI 1145 CAATTG C^AATTG
MunI 1145 CAATTG C^AATTG
NcoI 935 CCATGG C^CATGG
NdeI 166 CATATG CA^TATG
PflMI 838 CCACTAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 521 GAGTC GAGTC(4/5)
SacI 406 GAGCTC GAGCT^C
SapI 403 GAAGAGC GCTCTTC(1/4)
SspBI 1031 TGTACA T^GTACA
StyI 935 CCATGG C^C[AT][AT]GG
TfiI 783 GAATC G^A[AT]TC
Van91I 838 CCACTAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 601 CCAGTGTCCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 21 enzymes) #
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AcsI 320 AAATTC [GA]^AATT[CT]
907 GAATTT
ApoI 320 AAATTC [GA]^AATT[CT]
907 GAATTT
BanII 406 GAGCTC G[GA]GC[CT]^C
561 GAGCCC
BfaI 473 CTAG C^TAG
841 CTAG
BmyI 406 GAGCTC G[AGT]GC[ACT]^C
561 GAGCCC
BseRI 833 CTCCTC GAGGAG(10/8)
305 GAGGAG
Bsp1286I 406 GAGCTC G[AGT]GC[ACT]^C
561 GAGCCC
Cac8I 1080 GCTGGC GC[ACGT]^[ACGT]GC
1084 GCAAGC
HapII 38 CCGG C^CGG
388 CCGG
HinfI 521 GAGTC G^A[ACGT]TC
783 GAATC
HpaII 38 CCGG C^CGG
388 CCGG
HphI 904 GGTGA GGTGA(8/7)
932 TCACC
MaeI 473 CTAG C^TAG
841 CTAG
MslI 765 CACTGAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
893 CACATCAGTG
MspI 38 CCGG C^CGG
388 CCGG
MwoI 370 GCTGTGAGGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
1091 GCATCTTGAGC
NlaIII 348 CATG CATG^
936 CATG
SmlI 558 CTTGAG C^T[CT][GA]AG
1095 CTTGAG
TaqI 303 TCGA T^CGA
324 TCGA
Tsp45I 94 GTCAC ^GT[GC]AC
865 GTGAC
TthHB8I 303 TCGA T^CGA
324 TCGA
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# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AvaII BsaJI BsiYI BslI BsmFI
BsrI BssKI BstF5I BstNI Cfr13I
DdeI DpnII DraII EarI EcoO109I
EcoRII FokI Ksp632I MaeIII MboI
MboII MnlI MseI MvaI NdeII
NlaIV PpuMI RsaI Sau3AI Sau96I
ScrFI SfaNI Tru9I Tsp509I TspRI