(Circular) (Six-base) MAPSORT of: pFN21AB0936.seq Check: 6287 from: 1 to: 5863 pFN21AB0936 5863 bp With 182 enzymes: SgfI * April 15, 2010 09:33 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3673 278 Size: 53 83 186 936 2137 2468 Fragments arranged by size: 2468 2137 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3206 1937 Size: 1269 4594 Acc65I G'GTAC_C Cuts at: 3184 3184 Size: 5863 AclI AA'CG_TT Cuts at: 1563 3990 4363 1563 Size: 2427 373 3063 Fragments arranged by size: 3063 2427 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2216 2793 3926 5310 5578 1632 Size: 584 577 1133 1384 268 1917 Fragments arranged by size: 1917 1384 1133 584 577 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5666 AflIII A'CryG_T Cuts at: 1203 4762 5739 1203 Size: 3559 977 1327 Fragments arranged by size: 3559 1327 977 AgeI A'CCGG_T Cuts at: 4694 4694 Size: 5863 AhdI GACnn_n'nnGTC Cuts at: 4592 4592 Size: 5863 AleI CACnn'nnGTG Cuts at: 1166 2774 2902 1166 Size: 1608 128 4127 Fragments arranged by size: 4127 1608 128 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5863 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2587 2619 2587 Size: 32 5831 AlwNI CAG_nnn'CTG Cuts at: 2239 2740 3026 3606 5178 2239 Size: 501 286 580 1572 2924 Fragments arranged by size: 2924 1572 580 501 286 ApaLI G'TGCA_C Cuts at: 3920 5076 3920 Size: 1156 4707 ApoI r'AATT_y Cuts at: 1493 2325 2912 3172 3398 3550 5659 1493 Size: 832 587 260 226 152 2109 1697 Fragments arranged by size: 2109 1697 832 587 260 226 152 AseI AT'TA_AT Cuts at: 160 1032 4417 160 Size: 872 3385 1606 Fragments arranged by size: 3385 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5863 AvaI C'yCGr_G Cuts at: 1870 1943 3188 1870 Size: 73 1245 4545 Fragments arranged by size: 4545 1245 73 BamHI G'GATC_C Cuts at: 3193 3560 3193 Size: 367 5496 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3184 4639 618 Size: 325 217 627 1397 1455 1842 Fragments arranged by size: 1842 1455 1397 627 325 217 BanII G_rGCy'C Cuts at: 1391 2415 2570 3182 1391 Size: 1024 155 612 4072 Fragments arranged by size: 4072 1024 612 155 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4892 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4054 4088 1903 Size: 34 2117 34 3678 Fragments arranged by size: 3678 2117 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3755 4965 3755 Size: 1210 4653 BclI T'GATC_A Cuts at: 1548 1548 Size: 5863 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4474 136 Size: 107 122 71 631 605 2802 1525 Fragments arranged by size: 2802 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5858 5858 Size: 5863 BlpI GC'TnA_GC Cuts at: 3280 3280 Size: 5863 Bme1580I G_kGCm'C Cuts at: 1790 3924 5080 1790 Size: 2134 1156 2573 Fragments arranged by size: 2573 2134 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5863 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2564 2667 3014 Size: 416 53 83 399 24 1147 103 347 Cuts at: 3014 3594 4552 442 Size: 580 958 1753 Fragments arranged by size: 1753 1147 958 580 416 399 347 103 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5863 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2209 2403 2209 Size: 194 5669 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2984 4523 1239 Size: 1745 1539 2579 Fragments arranged by size: 2579 1745 1539 Bpu10I CC'TnA_GC Cuts at: 3081 3081 Size: 5863 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2584 3121 3988 4853 5151 2584 Size: 537 867 865 298 3296 Fragments arranged by size: 3296 867 865 537 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4526 882 Size: 617 3027 2219 Fragments arranged by size: 3027 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3654 493 Size: 711 2450 2702 Fragments arranged by size: 2702 2450 711 BsaBI GATnn'nnATC Cuts at: 5857 5857 Size: 5863 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3670 4052 4687 275 Size: 53 83 186 936 2137 382 635 1451 Fragments arranged by size: 2137 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2042 4296 4694 4968 5115 5526 2042 Size: 2254 398 274 147 411 2379 Fragments arranged by size: 2379 2254 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2012 2042 1178 Size: 30 165 30 609 30 4999 Fragments arranged by size: 4999 609 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2325 2829 1186 Size: 1139 504 4220 Fragments arranged by size: 4220 1139 504 BseYI C'CCAG_C Cuts at: 5066 5562 5066 Size: 496 5367 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5290 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3313 4074 5102 1540 Size: 275 186 1312 761 1028 2301 Fragments arranged by size: 2301 1312 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2415 3924 4009 5080 2415 Size: 1509 85 1071 3198 Fragments arranged by size: 3198 1509 1071 85 BsmI GAATG_Cn' Cuts at: 2883 3374 3467 2883 Size: 491 93 5279 Fragments arranged by size: 5279 491 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2415 2570 3182 3924 4009 5080 1391 Size: 399 625 155 612 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 625 612 399 155 85 BspEI T'CCGG_A Cuts at: 5526 5526 Size: 5863 BspHI T'CATG_A Cuts at: 3752 5482 3752 Size: 1730 4133 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2725 3218 844 Size: 1881 493 3489 Fragments arranged by size: 3489 1881 493 BsrBI CCG'CTC Cuts at: 1394 1823 3750 1394 Size: 429 1927 3507 Fragments arranged by size: 3507 1927 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2243 4358 4532 65 Size: 1183 995 2115 174 1396 Fragments arranged by size: 2115 1396 1183 995 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2392 4507 4694 1700 Size: 110 582 2115 187 2869 Fragments arranged by size: 2869 2115 582 187 110 BsrGI T'GTAC_A Cuts at: 96 3036 96 Size: 2940 2923 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5863 BssSI C'ACGA_G Cuts at: 3923 4935 3923 Size: 1012 4851 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5863 BstBI TT'CG_AA Cuts at: 4667 4667 Size: 5863 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5863 BstXI CCAn_nnnn'nTGG Cuts at: 2143 2143 Size: 5863 BstYI r'GATC_y Cuts at: 1972 3193 3560 3945 3962 5414 5500 5512 5858 Size: 1221 367 385 17 1452 86 12 346 Cuts at: 5858 1972 Size: 1977 Fragments arranged by size: 1977 1452 1221 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2940 513 Size: 552 1875 3436 Fragments arranged by size: 3436 1875 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5124 Fragments arranged by size: 5124 683 56 BtsI GCAGTG_nn' Cuts at: 2283 3379 4173 4193 2283 Size: 1096 794 20 3953 Fragments arranged by size: 3953 1096 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5863 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2046 2081 529 Size: 35 1482 35 4311 Fragments arranged by size: 4311 1482 35 35 DraI TTT'AAA Cuts at: 2118 3167 2118 Size: 1049 4814 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2096 4870 809 Size: 507 780 2774 1802 Fragments arranged by size: 2774 1802 780 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3310 4199 5828 Size: 54 1425 50 275 18 1480 889 1629 Cuts at: 5828 8 Size: 43 Fragments arranged by size: 1629 1480 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3310 1537 Size: 275 1498 4090 Fragments arranged by size: 4090 1498 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2235 2403 3124 3129 3793 1353 Size: 882 168 721 5 664 3423 Fragments arranged by size: 3423 882 721 664 168 5 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4450 4824 4970 387 Size: 1064 138 2861 374 146 1280 Fragments arranged by size: 2861 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2413 2413 Size: 5863 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2216 2793 2984 3926 4523 5310 5578 Size: 393 584 577 191 942 597 787 268 Cuts at: 5578 1239 Size: 1524 Fragments arranged by size: 1524 942 787 597 584 577 393 268 191 EcoO109I rG'GnC_Cy Cuts at: 2458 2576 2737 2458 Size: 118 161 5584 Fragments arranged by size: 5584 161 118 EcoRI G'AATT_C Cuts at: 3172 3550 3172 Size: 378 5485 EcoRV GAT'ATC Cuts at: 3118 3118 Size: 5863 FspI TGC'GCA Cuts at: 4369 4369 Size: 5863 HaeII r_GCGC'y Cuts at: 1678 5010 1678 Size: 3332 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4504 4536 4578 4610 1373 Size: 32 135 32 2932 32 42 32 2626 Fragments arranged by size: 2932 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3207 3453 669 Size: 988 281 1269 246 3079 Fragments arranged by size: 3079 1269 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5863 HpaI GTT'AAC Cuts at: 3453 3453 Size: 5863 KpnI G_GTAC'C Cuts at: 3188 3188 Size: 5863 MfeI C'AATT_G Cuts at: 3150 3462 3150 Size: 312 5551 MluI A'CGCG_T Cuts at: 5739 5739 Size: 5863 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3086 4977 5161 1305 Size: 1781 1891 184 2007 Fragments arranged by size: 2007 1891 1781 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4041 Fragments arranged by size: 4041 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2774 2902 3821 4180 4339 518 Size: 648 1608 128 919 359 159 2042 Fragments arranged by size: 2042 1608 919 648 359 159 128 MspA1I CmG'CkG Cuts at: 1606 3279 3956 5104 5349 1606 Size: 1673 677 1148 245 2120 Fragments arranged by size: 2120 1673 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2940 513 Size: 552 1875 3436 Fragments arranged by size: 3436 1875 552 NdeI CA'TA_TG Cuts at: 387 2172 387 Size: 1785 4078 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5863 NotI GC'GGCC_GC Cuts at: 3310 3310 Size: 5863 NspI r_CATG'y Cuts at: 1132 1229 3221 4766 1132 Size: 97 1992 1545 2229 Fragments arranged by size: 2229 1992 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5863 PciI A'CATG_T Cuts at: 4762 4762 Size: 5863 PflMI CCAn_nnn'nTGG Cuts at: 1108 2849 1108 Size: 1741 4122 PfoI T'CCnGG_A Cuts at: 2863 2863 Size: 5863 PmeI GTTT'AAAC Cuts at: 3167 3167 Size: 5863 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3932 3964 5445 5477 3932 Size: 32 1481 32 4318 Fragments arranged by size: 4318 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2458 2576 2737 2458 Size: 118 161 5584 Fragments arranged by size: 5584 161 118 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5863 PsiI TTA'TAA Cuts at: 3433 3433 Size: 5863 PstI C_TGCA'G Cuts at: 830 3215 830 Size: 2385 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5863 SacI G_AGCT'C Cuts at: 2415 2415 Size: 5863 SalI G'TCGA_C Cuts at: 1936 3205 1936 Size: 1269 4594 SanDI GG'GwC_CC Cuts at: 2458 2458 Size: 5863 SapI GCTCTTCn'nnn_ Cuts at: 2403 2403 Size: 5863 SbfI CC_TGCA'GG Cuts at: 3215 3215 Size: 5863 ScaI AGT'ACT Cuts at: 4111 5823 4111 Size: 1712 4151 SfcI C'TryA_G Cuts at: 826 1045 3211 4346 5027 5218 826 Size: 219 2166 1135 681 191 1471 Fragments arranged by size: 2166 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5863 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5863 SmaI CCC'GGG Cuts at: 1872 3190 1872 Size: 1318 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2563 3100 3967 4868 5130 820 Size: 197 926 620 537 867 901 262 1553 Fragments arranged by size: 1553 926 901 867 620 537 262 197 SnaBI TAC'GTA Cuts at: 493 3654 493 Size: 3161 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5863 SphI G_CATG'C Cuts at: 1132 3221 1132 Size: 2089 3774 SspI AAT'ATT Cuts at: 5 52 3787 5 Size: 47 3735 2081 Fragments arranged by size: 3735 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5863 StyI C'CwwG_G Cuts at: 513 1065 2940 3302 513 Size: 552 1875 362 3074 Fragments arranged by size: 3074 1875 552 362 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2715 3890 3907 4060 4245 4690 325 Size: 1255 1135 1175 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1175 1135 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2557 3036 4109 Size: 274 80 33 51 1443 580 479 1073 Cuts at: 4109 5821 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1073 580 479 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5823 5855 5823 Size: 32 5831 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5863 XbaI T'CTAG_A Cuts at: 3199 3199 Size: 5863 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2613 2613 Size: 5863 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5863 XmaI C'CCGG_G Cuts at: 1870 3188 1870 Size: 1318 4545 XmnI GAAnn'nnTTC Cuts at: 3992 3992 Size: 5863 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3671 276 Size: 53 83 186 936 2137 2468 Fragments arranged by size: 2468 2137 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacII SexAI SfiI SfoI SrfI SwaI