(Circular) (Six-base) MAPSORT of: pFN21AB0407.seq Check: 5262 from: 1 to: 6100 pFN21AB0407 6100 bp With 182 enzymes: SgfI * April 15, 2010 09:33 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2516 3065 2516 Size: 549 5551 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2736 3910 278 Size: 53 83 186 936 1200 1174 2468 Fragments arranged by size: 2468 1200 1174 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3443 1937 Size: 1506 4594 Acc65I G'GTAC_C Cuts at: 2946 3421 2946 Size: 475 5625 AclI AA'CG_TT Cuts at: 1563 4227 4600 1563 Size: 2664 373 3063 Fragments arranged by size: 3063 2664 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2899 4163 5547 5815 1632 Size: 1267 1264 1384 268 1917 Fragments arranged by size: 1917 1384 1267 1264 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5903 AflIII A'CryG_T Cuts at: 1203 4999 5976 1203 Size: 3796 977 1327 Fragments arranged by size: 3796 1327 977 AgeI A'CCGG_T Cuts at: 2943 4931 2943 Size: 1988 4112 AhdI GACnn_n'nnGTC Cuts at: 2538 4829 2538 Size: 2291 3809 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6100 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2976 1184 Size: 1792 4308 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3374 3406 3374 Size: 32 6068 AlwNI CAG_nnn'CTG Cuts at: 2508 3843 5415 2508 Size: 1335 1572 3193 Fragments arranged by size: 3193 1572 1335 ApaLI G'TGCA_C Cuts at: 2725 4157 5313 2725 Size: 1432 1156 3512 Fragments arranged by size: 3512 1432 1156 ApoI r'AATT_y Cuts at: 1493 2624 3409 3635 3787 5896 1493 Size: 1131 785 226 152 2109 1697 Fragments arranged by size: 2109 1697 1131 785 226 152 AseI AT'TA_AT Cuts at: 160 1032 4654 160 Size: 872 3622 1606 Fragments arranged by size: 3622 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6100 AvaI C'yCGr_G Cuts at: 1870 1943 2313 3332 3425 1870 Size: 73 370 1019 93 4545 Fragments arranged by size: 4545 1019 370 93 73 BamHI G'GATC_C Cuts at: 3430 3797 3430 Size: 367 5733 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2946 3421 4876 618 Size: 325 217 627 1159 475 1455 1842 Fragments arranged by size: 1842 1455 1159 627 475 325 217 BanII G_rGCy'C Cuts at: 1391 2301 2744 2907 3419 1391 Size: 910 443 163 512 4072 Fragments arranged by size: 4072 910 512 443 163 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5129 BbvCI CC'TCA_GC Cuts at: 2153 2324 2153 Size: 171 5929 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4291 4325 1903 Size: 34 2354 34 3678 Fragments arranged by size: 3678 2354 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3992 5202 3992 Size: 1210 4890 BclI T'GATC_A Cuts at: 1548 1548 Size: 6100 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3203 4711 136 Size: 107 122 71 631 605 1531 1508 1525 Fragments arranged by size: 1531 1525 1508 631 605 122 107 71 BglII A'GATC_T Cuts at: 6095 6095 Size: 6100 BlpI GC'TnA_GC Cuts at: 2097 3517 2097 Size: 1420 4680 Bme1580I G_kGCm'C Cuts at: 1790 2729 4161 5317 1790 Size: 939 1432 1156 2573 Fragments arranged by size: 2573 1432 1156 939 BmgBI CAC'GTC Cuts at: 1318 3397 1318 Size: 2079 4021 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2039 3316 3831 Size: 416 53 83 399 24 622 1277 515 Cuts at: 3831 4789 442 Size: 958 1753 Fragments arranged by size: 1753 1277 958 622 515 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6100 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2328 2328 Size: 6100 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2014 2274 4760 1239 Size: 775 260 2486 2579 Fragments arranged by size: 2579 2486 775 260 Bpu10I CC'TnA_GC Cuts at: 2153 2324 2153 Size: 171 5929 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2066 4225 5090 5388 2066 Size: 2159 865 298 2778 Fragments arranged by size: 2778 2159 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2212 4763 882 Size: 617 713 2551 2219 Fragments arranged by size: 2551 2219 713 617 BsaAI yAC'GTr Cuts at: 493 1204 2724 2776 3891 493 Size: 711 1520 52 1115 2702 Fragments arranged by size: 2702 1520 1115 711 52 BsaBI GATnn'nnATC Cuts at: 6094 6094 Size: 6100 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2733 3907 4289 4924 Size: 53 83 186 936 1200 1174 382 635 Cuts at: 4924 275 Size: 1451 Fragments arranged by size: 1451 1200 1174 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2574 2943 4533 4931 5205 5352 5763 2574 Size: 369 1590 398 274 147 411 2911 Fragments arranged by size: 2911 1590 411 398 369 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2218 2248 2337 2367 1178 Size: 30 165 30 815 30 89 30 4911 Fragments arranged by size: 4911 815 165 89 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2981 1186 Size: 1795 4305 BseYI C'CCAG_C Cuts at: 2550 5303 5799 2550 Size: 2753 496 2851 Fragments arranged by size: 2851 2753 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2192 2234 2357 2495 2521 2640 2982 Size: 573 456 42 123 138 26 119 342 Cuts at: 2982 3154 3346 1163 Size: 172 192 3917 Fragments arranged by size: 3917 573 456 342 192 172 138 123 119 42 26 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3550 4311 5339 1540 Size: 275 186 1549 761 1028 2301 Fragments arranged by size: 2301 1549 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2647 2729 2907 4161 4246 5317 2647 Size: 82 178 1254 85 1071 3430 Fragments arranged by size: 3430 1254 1071 178 85 82 BsmI GAATG_Cn' Cuts at: 2251 2594 3611 3704 2251 Size: 343 1017 93 4647 Fragments arranged by size: 4647 1017 343 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2301 2647 2729 2744 2907 3419 4161 Size: 399 511 346 82 15 163 512 742 Cuts at: 4161 4246 5317 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 512 511 399 346 163 85 82 15 BspEI T'CCGG_A Cuts at: 5763 5763 Size: 6100 BspHI T'CATG_A Cuts at: 3989 5719 3989 Size: 1730 4370 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2408 2516 3065 3455 844 Size: 1564 108 549 390 3489 Fragments arranged by size: 3489 1564 549 390 108 BsrBI CCG'CTC Cuts at: 1394 1823 2293 3020 3987 1394 Size: 429 470 727 967 3507 Fragments arranged by size: 3507 967 727 470 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3076 4595 4769 65 Size: 1183 1828 1519 174 1396 Fragments arranged by size: 1828 1519 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2943 4744 4931 1700 Size: 110 1133 1801 187 2869 Fragments arranged by size: 2869 1801 1133 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6100 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6100 BssSI C'ACGA_G Cuts at: 4160 5172 4160 Size: 1012 5088 BstAPI GCAn_nnn'nTGC Cuts at: 794 2508 2724 794 Size: 1714 216 4170 Fragments arranged by size: 4170 1714 216 BstBI TT'CG_AA Cuts at: 4904 4904 Size: 6100 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6100 BstXI CCAn_nnnn'nTGG Cuts at: 2550 2957 2550 Size: 407 5693 BstYI r'GATC_y Cuts at: 1972 3430 3797 4182 4199 5651 5737 5749 6095 Size: 1458 367 385 17 1452 86 12 346 Cuts at: 6095 1972 Size: 1977 Fragments arranged by size: 1977 1458 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3154 3154 Size: 6100 BtgI C'CryG_G Cuts at: 513 1065 2035 2222 513 Size: 552 970 187 4391 Fragments arranged by size: 4391 970 552 187 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5361 Fragments arranged by size: 5361 683 56 BtsI GCAGTG_nn' Cuts at: 2602 3142 3616 4410 4430 2602 Size: 540 474 794 20 4272 Fragments arranged by size: 4272 794 540 474 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6100 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2762 2797 529 Size: 35 2198 35 3832 Fragments arranged by size: 3832 2198 35 35 DraI TTT'AAA Cuts at: 2635 3404 2635 Size: 769 5331 DraIII CAC_nnn'GTG Cuts at: 2212 2212 Size: 6100 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5107 809 Size: 507 3791 1802 Fragments arranged by size: 3791 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3547 4436 6065 Size: 54 1425 50 275 18 1717 889 1629 Cuts at: 6065 8 Size: 43 Fragments arranged by size: 1717 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3547 1537 Size: 275 1735 4090 Fragments arranged by size: 4090 1735 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2925 4030 1353 Size: 1572 1105 3423 Fragments arranged by size: 3423 1572 1105 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4687 5061 5207 387 Size: 1064 138 3098 374 146 1280 Fragments arranged by size: 3098 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2905 2905 Size: 6100 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2014 2274 2899 4163 4760 5547 5815 Size: 393 382 260 625 1264 597 787 268 Cuts at: 5815 1239 Size: 1524 Fragments arranged by size: 1524 1264 787 625 597 393 382 268 260 EcoO109I rG'GnC_Cy Cuts at: 2459 2459 Size: 6100 EcoRI G'AATT_C Cuts at: 3409 3787 3409 Size: 378 5722 FspI TGC'GCA Cuts at: 4606 4606 Size: 6100 HaeII r_GCGC'y Cuts at: 1678 5247 1678 Size: 3569 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2218 2250 2524 2556 4741 Size: 32 135 32 646 32 274 32 2185 Cuts at: 4741 4773 4815 4847 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2185 646 274 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3444 3690 669 Size: 988 281 1506 246 3079 Fragments arranged by size: 3079 1506 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6100 HpaI GTT'AAC Cuts at: 3690 3690 Size: 6100 KpnI G_GTAC'C Cuts at: 2950 3425 2950 Size: 475 5625 MfeI C'AATT_G Cuts at: 3699 3699 Size: 6100 MluI A'CGCG_T Cuts at: 5976 5976 Size: 6100 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2684 5214 5398 1305 Size: 1379 2530 184 2007 Fragments arranged by size: 2530 2007 1379 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4278 Fragments arranged by size: 4278 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 3302 4058 4417 4576 518 Size: 648 843 1293 756 359 159 2042 Fragments arranged by size: 2042 1293 843 756 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2405 3264 3516 4193 5341 5586 1606 Size: 799 859 252 677 1148 245 2120 Fragments arranged by size: 2120 1148 859 799 677 252 245 NcoI C'CATG_G Cuts at: 513 1065 2035 513 Size: 552 970 4578 Fragments arranged by size: 4578 970 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6100 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6100 NotI GC'GGCC_GC Cuts at: 3547 3547 Size: 6100 NspI r_CATG'y Cuts at: 1132 1229 3301 3458 5003 1132 Size: 97 2072 157 1545 2229 Fragments arranged by size: 2229 2072 1545 157 97 PasI CC'CwG_GG Cuts at: 1348 2536 2609 1348 Size: 1188 73 4839 Fragments arranged by size: 4839 1188 73 PciI A'CATG_T Cuts at: 4999 4999 Size: 6100 PflMI CCAn_nnn'nTGG Cuts at: 1108 2405 1108 Size: 1297 4803 PmeI GTTT'AAAC Cuts at: 3404 3404 Size: 6100 PmlI CAC'GTG Cuts at: 2776 2776 Size: 6100 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3151 3183 4169 4201 5682 5714 3151 Size: 32 986 32 1481 32 3537 Fragments arranged by size: 3537 1481 986 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6100 PsiI TTA'TAA Cuts at: 3670 3670 Size: 6100 PspXI vC'TCGA_Gb Cuts at: 3332 3332 Size: 6100 PstI C_TGCA'G Cuts at: 830 2513 2982 3452 830 Size: 1683 469 470 3478 Fragments arranged by size: 3478 1683 470 469 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6100 PvuII CAG'CTG Cuts at: 2405 2405 Size: 6100 SacI G_AGCT'C Cuts at: 2907 2907 Size: 6100 SalI G'TCGA_C Cuts at: 1936 3442 1936 Size: 1506 4594 SapI GCTCTTCn'nnn_ Cuts at: 2925 2925 Size: 6100 SbfI CC_TGCA'GG Cuts at: 2513 3452 2513 Size: 939 5161 ScaI AGT'ACT Cuts at: 4348 6060 4348 Size: 1712 4388 SfcI C'TryA_G Cuts at: 826 1045 2509 2978 3175 3448 4583 5264 5455 Size: 219 1464 469 197 273 1135 681 191 Cuts at: 5455 826 Size: 1471 Fragments arranged by size: 1471 1464 1135 681 469 273 219 197 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6100 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6100 SmaI CCC'GGG Cuts at: 1872 3427 1872 Size: 1555 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2081 3332 4204 5105 5367 820 Size: 197 926 138 1251 872 901 262 1553 Fragments arranged by size: 1553 1251 926 901 872 262 197 138 SnaBI TAC'GTA Cuts at: 493 3891 493 Size: 3398 2702 SpeI A'CTAG_T Cuts at: 152 2615 152 Size: 2463 3637 SphI G_CATG'C Cuts at: 1132 3301 3458 1132 Size: 2169 157 3774 Fragments arranged by size: 3774 2169 157 SspI AAT'ATT Cuts at: 5 52 4024 5 Size: 47 3972 2081 Fragments arranged by size: 3972 2081 47 StuI AGG'CCT Cuts at: 1517 3173 1517 Size: 1656 4444 StyI C'CwwG_G Cuts at: 513 1065 2035 3539 513 Size: 552 970 1504 3074 Fragments arranged by size: 3074 1504 970 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4127 4144 4297 4482 4927 325 Size: 1255 2547 17 153 185 445 1498 Fragments arranged by size: 2547 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2937 4346 6058 Size: 274 80 33 51 1443 960 1409 1712 Cuts at: 6058 96 Size: 138 Fragments arranged by size: 1712 1443 1409 960 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2337 2369 2569 2601 6060 6092 2337 Size: 32 200 32 3459 32 2345 Fragments arranged by size: 3459 2345 200 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6100 XbaI T'CTAG_A Cuts at: 3436 3436 Size: 6100 XhoI C'TCGA_G Cuts at: 1943 3332 1943 Size: 1389 4711 XmaI C'CCGG_G Cuts at: 1870 3425 1870 Size: 1555 4545 XmnI GAAnn'nnTTC Cuts at: 4229 4229 Size: 6100 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2734 3908 276 Size: 53 83 186 936 1200 1174 2468 Fragments arranged by size: 2468 1200 1174 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspXI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PpuMI PspOMI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI