(Circular) (Six-base) MAPSORT of: pFN21AB8508.seq Check: 5212 from: 1 to: 7303 pFN21AB8508 7303 bp With 182 enzymes: SgfI * April 15, 2010 09:33 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5113 278 Size: 53 83 186 936 3577 2468 Fragments arranged by size: 3577 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4646 1937 Size: 2709 4594 Acc65I G'GTAC_C Cuts at: 2734 3929 4624 2734 Size: 1195 695 5413 Fragments arranged by size: 5413 1195 695 AclI AA'CG_TT Cuts at: 1563 5430 5803 1563 Size: 3867 373 3063 Fragments arranged by size: 3867 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2112 2447 2606 2711 3909 4269 5366 6750 Size: 480 335 159 105 1198 360 1097 1384 Cuts at: 6750 7018 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 1198 1097 480 360 335 268 159 105 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7106 AflIII A'CryG_T Cuts at: 1203 3374 6202 7179 1203 Size: 2171 2828 977 1327 Fragments arranged by size: 2828 2171 1327 977 AgeI A'CCGG_T Cuts at: 6134 6134 Size: 7303 AhdI GACnn_n'nnGTC Cuts at: 6032 6032 Size: 7303 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7303 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7303 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3070 3102 3070 Size: 32 7271 AlwNI CAG_nnn'CTG Cuts at: 2467 3203 3656 3897 5046 6618 2467 Size: 736 453 241 1149 1572 3152 Fragments arranged by size: 3152 1572 1149 736 453 241 ApaLI G'TGCA_C Cuts at: 5360 6516 5360 Size: 1156 6147 ApoI r'AATT_y Cuts at: 1493 2272 2337 2710 3710 4054 4121 4612 4838 Size: 779 65 373 1000 344 67 491 226 Cuts at: 4838 4990 7099 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 1000 779 491 373 344 226 152 67 65 AseI AT'TA_AT Cuts at: 160 1032 5857 160 Size: 872 4825 1606 Fragments arranged by size: 4825 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7303 AvaI C'yCGr_G Cuts at: 1870 1943 2814 3479 4628 1870 Size: 73 871 665 1149 4545 Fragments arranged by size: 4545 1149 871 665 73 BamHI G'GATC_C Cuts at: 3965 4633 5000 3965 Size: 668 367 6268 Fragments arranged by size: 6268 668 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2734 3540 3929 4624 6079 Size: 325 217 627 947 806 389 695 1455 Cuts at: 6079 618 Size: 1842 Fragments arranged by size: 1842 1455 947 806 695 627 389 325 217 BanII G_rGCy'C Cuts at: 1391 2488 2903 2942 2981 3020 3059 3137 3330 Size: 1097 415 39 39 39 39 78 193 Cuts at: 3330 3650 4169 4481 4622 1391 Size: 320 519 312 141 4072 Fragments arranged by size: 4072 1097 519 415 320 312 193 141 78 39 39 39 39 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2184 928 Size: 971 285 6047 Fragments arranged by size: 6047 971 285 BbvCI CC'TCA_GC Cuts at: 2359 2359 Size: 7303 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2535 2569 5494 5528 1903 Size: 34 598 34 2925 34 3678 Fragments arranged by size: 3678 2925 598 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5195 6405 5195 Size: 1210 6093 BclI T'GATC_A Cuts at: 1548 1548 Size: 7303 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2202 5914 136 Size: 107 122 71 631 605 530 3712 1525 Fragments arranged by size: 3712 1525 631 605 530 122 107 71 BglII A'GATC_T Cuts at: 2581 7298 2581 Size: 4717 2586 BlpI GC'TnA_GC Cuts at: 4720 4720 Size: 7303 Bme1580I G_kGCm'C Cuts at: 1790 2051 3120 3239 5364 6520 1790 Size: 261 1069 119 2125 1156 2573 Fragments arranged by size: 2573 2125 1156 1069 261 119 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7303 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3241 4204 5034 Size: 416 53 83 399 24 1824 963 830 Cuts at: 5034 5992 442 Size: 958 1753 Fragments arranged by size: 1824 1753 963 958 830 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7303 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4354 4354 Size: 7303 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2531 2970 5963 1239 Size: 1292 439 2993 2579 Fragments arranged by size: 2993 2579 1292 439 Bpu10I CC'TnA_GC Cuts at: 2359 3329 2359 Size: 970 6333 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5428 6293 6591 5428 Size: 865 298 6140 Fragments arranged by size: 6140 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2785 3995 5966 882 Size: 617 1286 1210 1971 2219 Fragments arranged by size: 2219 1971 1286 1210 617 BsaAI yAC'GTr Cuts at: 493 1204 5094 493 Size: 711 3890 2702 Fragments arranged by size: 3890 2702 711 BsaBI GATnn'nnATC Cuts at: 7297 7297 Size: 7303 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5110 5492 6127 275 Size: 53 83 186 936 3577 382 635 1451 Fragments arranged by size: 3577 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2840 3074 4062 5736 6134 6408 6555 6966 2840 Size: 234 988 1674 398 274 147 411 3177 Fragments arranged by size: 3177 1674 988 411 398 274 234 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2088 2118 3609 3639 1178 Size: 30 165 30 685 30 1491 30 4842 Fragments arranged by size: 4842 1491 685 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2112 2832 3792 4261 1186 Size: 926 720 960 469 4228 Fragments arranged by size: 4228 960 926 720 469 BseYI C'CCAG_C Cuts at: 3113 4199 6506 7002 3113 Size: 1086 2307 496 3414 Fragments arranged by size: 3414 2307 1086 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 4422 1163 Size: 573 2686 4044 Fragments arranged by size: 4044 2686 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4753 5514 6542 1540 Size: 275 186 2752 761 1028 2301 Fragments arranged by size: 2752 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2488 2903 2942 2981 3020 3137 3330 5364 5449 Size: 415 39 39 39 117 193 2034 85 Cuts at: 5449 6520 2488 Size: 1071 3271 Fragments arranged by size: 3271 2034 1071 415 193 117 85 39 39 39 BsmI GAATG_Cn' Cuts at: 4814 4907 4814 Size: 93 7210 BsmBI CGTCTCn'nnnn_ Cuts at: 2843 2843 Size: 7303 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2051 2488 2903 2942 2981 3020 3059 Size: 399 261 437 415 39 39 39 39 Cuts at: 3059 3120 3137 3239 3330 3650 4169 4481 4622 Size: 61 17 102 91 320 519 312 141 Cuts at: 4622 5364 5449 6520 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 519 437 415 399 320 312 261 141 102 91 85 61 39 39 39 39 17 BspEI T'CCGG_A Cuts at: 6966 6966 Size: 7303 BspHI T'CATG_A Cuts at: 3841 5192 6922 3841 Size: 1351 1730 4222 Fragments arranged by size: 4222 1730 1351 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4658 844 Size: 3814 3489 BsrBI CCG'CTC Cuts at: 1394 1823 5190 1394 Size: 429 3367 3507 Fragments arranged by size: 3507 3367 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2625 3492 4141 5798 5972 65 Size: 1183 1377 867 649 1657 174 1396 Fragments arranged by size: 1657 1396 1377 1183 867 649 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5947 6134 1700 Size: 110 4137 187 2869 Fragments arranged by size: 4137 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7303 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7303 BssSI C'ACGA_G Cuts at: 2571 3763 5363 6375 2571 Size: 1192 1600 1012 3499 Fragments arranged by size: 3499 1600 1192 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2060 794 Size: 1266 6037 BstBI TT'CG_AA Cuts at: 6107 6107 Size: 7303 BstEII G'GTnAC_C Cuts at: 1184 3349 3451 1184 Size: 2165 102 5036 Fragments arranged by size: 5036 2165 102 BstXI CCAn_nnnn'nTGG Cuts at: 3050 3448 3050 Size: 398 6905 BstYI r'GATC_y Cuts at: 1972 2401 2532 2581 3965 4633 5000 5385 5402 Size: 429 131 49 1384 668 367 385 17 Cuts at: 5402 6854 6940 6952 7298 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1384 668 429 385 367 346 131 86 49 17 12 Bsu36I CC'TnA_GG Cuts at: 4562 4562 Size: 7303 BtgI C'CryG_G Cuts at: 513 1065 3721 513 Size: 552 2656 4095 Fragments arranged by size: 4095 2656 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6564 Fragments arranged by size: 6564 683 56 BtsI GCAGTG_nn' Cuts at: 2881 3567 4284 4819 5613 5633 2881 Size: 686 717 535 794 20 4551 Fragments arranged by size: 4551 794 717 686 535 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7303 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2103 2138 529 Size: 35 1539 35 5694 Fragments arranged by size: 5694 1539 35 35 DraI TTT'AAA Cuts at: 4607 4607 Size: 7303 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6310 809 Size: 507 4994 1802 Fragments arranged by size: 4994 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4750 5639 7268 Size: 54 1425 50 275 18 2920 889 1629 Cuts at: 7268 8 Size: 43 Fragments arranged by size: 2920 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4750 1537 Size: 275 2938 4090 Fragments arranged by size: 4090 2938 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3240 4386 5233 1353 Size: 1887 1146 847 3423 Fragments arranged by size: 3423 1887 1146 847 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3521 5890 6264 6410 387 Size: 1064 138 1932 2369 374 146 1280 Fragments arranged by size: 2369 1932 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2486 2901 2940 2979 3018 3135 3328 2486 Size: 415 39 39 39 117 193 6461 Fragments arranged by size: 6461 415 193 117 39 39 39 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2112 2447 2531 2606 2711 2970 3909 Size: 393 480 335 84 75 105 259 939 Cuts at: 3909 4269 5366 5963 6750 7018 1239 Size: 360 1097 597 787 268 1524 Fragments arranged by size: 1524 1097 939 787 597 480 393 360 335 268 259 105 84 75 EcoNI CCTnn'n_nnAGG Cuts at: 3612 4369 3612 Size: 757 6546 EcoO109I rG'GnC_Cy Cuts at: 4587 4587 Size: 7303 EcoRI G'AATT_C Cuts at: 3710 4121 4612 4990 3710 Size: 411 491 378 6023 Fragments arranged by size: 6023 491 411 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2657 2657 Size: 7303 FspI TGC'GCA Cuts at: 5809 5809 Size: 7303 HaeII r_GCGC'y Cuts at: 1678 6450 1678 Size: 4772 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2342 2374 3557 3589 3609 Size: 32 135 32 770 32 1183 32 20 Cuts at: 3609 3623 3641 3655 5944 5976 6018 6050 1373 Size: 14 18 14 2289 32 42 32 2626 Fragments arranged by size: 2626 2289 1183 770 135 42 32 32 32 32 32 32 20 18 14 14 HincII GTy'rAC Cuts at: 669 1657 1938 2319 4647 4893 669 Size: 988 281 381 2328 246 3079 Fragments arranged by size: 3079 2328 988 381 281 246 HindIII A'AGCT_T Cuts at: 748 4209 748 Size: 3461 3842 HpaI GTT'AAC Cuts at: 4893 4893 Size: 7303 KpnI G_GTAC'C Cuts at: 2738 3933 4628 2738 Size: 1195 695 5413 Fragments arranged by size: 5413 1195 695 MfeI C'AATT_G Cuts at: 4902 4902 Size: 7303 MluI A'CGCG_T Cuts at: 3374 7179 3374 Size: 3805 3498 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6417 6601 1305 Size: 5112 184 2007 Fragments arranged by size: 5112 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5481 Fragments arranged by size: 5481 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3546 5261 5620 5779 518 Size: 648 2380 1715 359 159 2042 Fragments arranged by size: 2380 2042 1715 648 359 159 MspA1I CmG'CkG Cuts at: 1606 4719 5396 6544 6789 1606 Size: 3113 677 1148 245 2120 Fragments arranged by size: 3113 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6751 NdeI CA'TA_TG Cuts at: 387 387 Size: 7303 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7303 NotI GC'GGCC_GC Cuts at: 4750 4750 Size: 7303 NspI r_CATG'y Cuts at: 1132 1229 2767 4354 4661 6206 1132 Size: 97 1538 1587 307 1545 2229 Fragments arranged by size: 2229 1587 1545 1538 307 97 PasI CC'CwG_GG Cuts at: 1348 2933 3050 3698 4472 1348 Size: 1585 117 648 774 4179 Fragments arranged by size: 4179 1585 774 648 117 PciI A'CATG_T Cuts at: 6202 6202 Size: 7303 PflMI CCAn_nnn'nTGG Cuts at: 1108 3203 1108 Size: 2095 5208 PfoI T'CCnGG_A Cuts at: 2704 2704 Size: 7303 PmeI GTTT'AAAC Cuts at: 4607 4607 Size: 7303 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5372 5404 6885 6917 5372 Size: 32 1481 32 5758 Fragments arranged by size: 5758 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7303 PsiI TTA'TAA Cuts at: 4873 4873 Size: 7303 PspXI vC'TCGA_Gb Cuts at: 2814 2814 Size: 7303 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3016 3048 3016 Size: 32 7271 PstI C_TGCA'G Cuts at: 830 2065 2210 2472 4655 830 Size: 1235 145 262 2183 3478 Fragments arranged by size: 3478 2183 1235 262 145 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7303 SacI G_AGCT'C Cuts at: 2488 2903 2942 2981 3020 3137 3330 2488 Size: 415 39 39 39 117 193 6461 Fragments arranged by size: 6461 415 193 117 39 39 39 SalI G'TCGA_C Cuts at: 1936 4645 1936 Size: 2709 4594 SbfI CC_TGCA'GG Cuts at: 4655 4655 Size: 7303 ScaI AGT'ACT Cuts at: 2917 2995 3034 3151 5551 7263 2917 Size: 78 39 117 2400 1712 2957 Fragments arranged by size: 2957 2400 1712 117 78 39 SexAI A'CCwGG_T Cuts at: 2794 3064 2794 Size: 270 7033 SfcI C'TryA_G Cuts at: 826 1045 2061 2206 2468 4651 5786 6467 6658 Size: 219 1016 145 262 2183 1135 681 191 Cuts at: 6658 826 Size: 1471 Fragments arranged by size: 2183 1471 1135 1016 681 262 219 191 145 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7303 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7303 SmaI CCC'GGG Cuts at: 1872 4630 1872 Size: 2758 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2814 5407 6308 6570 820 Size: 197 926 871 2593 901 262 1553 Fragments arranged by size: 2593 1553 926 901 871 262 197 SnaBI TAC'GTA Cuts at: 493 5094 493 Size: 4601 2702 SpeI A'CTAG_T Cuts at: 152 3755 152 Size: 3603 3700 SphI G_CATG'C Cuts at: 1132 4661 1132 Size: 3529 3774 SspI AAT'ATT Cuts at: 5 52 5227 5 Size: 47 5175 2081 Fragments arranged by size: 5175 2081 47 StuI AGG'CCT Cuts at: 1517 3217 1517 Size: 1700 5603 StyI C'CwwG_G Cuts at: 513 1065 2114 4490 4742 513 Size: 552 1049 2376 252 3074 Fragments arranged by size: 3074 2376 1049 552 252 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5330 5347 5500 5685 6130 325 Size: 1255 3750 17 153 185 445 1498 Fragments arranged by size: 3750 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2449 2915 2993 Size: 274 80 33 51 1443 472 466 78 Cuts at: 2993 3032 3149 4332 5549 7261 96 Size: 39 117 1183 1217 1712 138 Fragments arranged by size: 1712 1443 1217 1183 472 466 274 138 117 80 78 51 39 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2088 2120 7263 7295 2088 Size: 32 5143 32 2096 Fragments arranged by size: 5143 2096 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3637 1316 Size: 2321 4982 XbaI T'CTAG_A Cuts at: 2648 4639 2648 Size: 1991 5312 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2329 2329 Size: 7303 XhoI C'TCGA_G Cuts at: 1943 2814 1943 Size: 871 6432 XmaI C'CCGG_G Cuts at: 1870 4628 1870 Size: 2758 4545 XmnI GAAnn'nnTTC Cuts at: 5432 5432 Size: 7303 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5111 276 Size: 53 83 186 936 3577 2468 Fragments arranged by size: 3577 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspXI PsrI PstI PvuI SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I DraIII EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PvuII RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI