(Circular) (Six-base) MAPSORT of: pFN21AB7676.seq Check: 584 from: 1 to: 5923 pFN21AB7676 5923 bp With 182 enzymes: SgfI * April 15, 2010 09:33 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3143 3733 278 Size: 53 83 186 936 1607 590 2468 Fragments arranged by size: 2468 1607 936 590 186 83 53 AccI GT'mk_AC Cuts at: 1937 2493 3011 3266 1937 Size: 556 518 255 4594 Fragments arranged by size: 4594 556 518 255 Acc65I G'GTAC_C Cuts at: 3244 3244 Size: 5923 AclI AA'CG_TT Cuts at: 1563 4050 4423 1563 Size: 2487 373 3063 Fragments arranged by size: 3063 2487 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3986 5370 5638 1632 Size: 2354 1384 268 1917 Fragments arranged by size: 2354 1917 1384 268 AfeI AGC'GCT Cuts at: 2345 2345 Size: 5923 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5726 AflIII A'CryG_T Cuts at: 1203 2438 4822 5799 1203 Size: 1235 2384 977 1327 Fragments arranged by size: 2384 1327 1235 977 AgeI A'CCGG_T Cuts at: 4754 4754 Size: 5923 AhdI GACnn_n'nnGTC Cuts at: 4652 4652 Size: 5923 AleI CACnn'nnGTG Cuts at: 1166 2999 1166 Size: 1833 4090 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5923 AlwNI CAG_nnn'CTG Cuts at: 3666 5238 3666 Size: 1572 4351 ApaI G_GGCC'C Cuts at: 2030 2702 3050 2030 Size: 672 348 4903 Fragments arranged by size: 4903 672 348 ApaLI G'TGCA_C Cuts at: 2036 2431 3980 5136 2036 Size: 395 1549 1156 2823 Fragments arranged by size: 2823 1549 1156 395 ApoI r'AATT_y Cuts at: 1493 3232 3458 3610 5719 1493 Size: 1739 226 152 2109 1697 Fragments arranged by size: 2109 1739 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4477 160 Size: 872 3445 1606 Fragments arranged by size: 3445 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5923 AvaI C'yCGr_G Cuts at: 1870 1943 2030 2397 2695 2785 2955 3248 1870 Size: 73 87 367 298 90 170 293 4545 Fragments arranged by size: 4545 367 298 293 170 90 87 73 BamHI G'GATC_C Cuts at: 3253 3620 3253 Size: 367 5556 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3244 4699 618 Size: 325 217 627 1457 1455 1842 Fragments arranged by size: 1842 1457 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2030 2277 2696 2702 3050 3063 3242 1391 Size: 639 247 419 6 348 13 179 4072 Fragments arranged by size: 4072 639 419 348 247 179 13 6 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2322 2627 2804 928 Size: 971 423 305 177 4047 Fragments arranged by size: 4047 971 423 305 177 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4114 4148 1903 Size: 34 50 34 2093 34 3678 Fragments arranged by size: 3678 2093 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3815 5025 3815 Size: 1210 4713 BclI T'GATC_A Cuts at: 1548 1548 Size: 5923 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2364 2561 2588 Size: 107 122 71 631 605 692 197 27 Cuts at: 2588 4534 136 Size: 1946 1525 Fragments arranged by size: 1946 1525 692 631 605 197 122 107 71 27 BglII A'GATC_T Cuts at: 2181 5918 2181 Size: 3737 2186 BlpI GC'TnA_GC Cuts at: 2735 3077 3340 2735 Size: 342 263 5318 Fragments arranged by size: 5318 342 263 Bme1580I G_kGCm'C Cuts at: 1790 2030 2040 2435 2660 2702 3050 3984 5140 Size: 240 10 395 225 42 348 934 1156 Cuts at: 5140 1790 Size: 2573 Fragments arranged by size: 2573 1156 934 395 348 240 225 42 10 BmgBI CAC'GTC Cuts at: 1318 2560 2817 1318 Size: 1242 257 4424 Fragments arranged by size: 4424 1242 257 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3654 4612 442 Size: 416 53 83 399 24 2237 958 1753 Fragments arranged by size: 2237 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5923 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2097 2097 Size: 5923 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2285 2918 3070 3191 4583 1239 Size: 1046 633 152 121 1392 2579 Fragments arranged by size: 2579 1392 1046 633 152 121 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2222 4048 4913 5211 2222 Size: 1826 865 298 2934 Fragments arranged by size: 2934 1826 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4586 882 Size: 617 3087 2219 Fragments arranged by size: 3087 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2430 2439 3714 493 Size: 711 1226 9 1275 2702 Fragments arranged by size: 2702 1275 1226 711 9 BsaBI GATnn'nnATC Cuts at: 5917 5917 Size: 5923 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2880 3038 3140 3730 Size: 53 83 186 936 1347 158 102 590 Cuts at: 3730 4112 4747 275 Size: 382 635 1451 Fragments arranged by size: 1451 1347 936 635 590 382 186 158 102 83 53 BsaWI w'CCGG_w Cuts at: 4356 4754 5028 5175 5586 4356 Size: 398 274 147 411 4693 Fragments arranged by size: 4693 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5698 Fragments arranged by size: 5698 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5923 BseYI C'CCAG_C Cuts at: 2605 5126 5622 2605 Size: 2521 496 2906 Fragments arranged by size: 2906 2521 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2360 2684 1163 Size: 573 624 324 4402 Fragments arranged by size: 4402 624 573 324 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2107 2574 2678 3373 4134 5162 Size: 275 186 106 467 104 695 761 1028 Cuts at: 5162 1540 Size: 2301 Fragments arranged by size: 2301 1028 761 695 467 275 186 106 104 BsiHKAI G_wGCw'C Cuts at: 2040 2277 2435 2763 3063 3984 4069 5140 2040 Size: 237 158 328 300 921 85 1071 2823 Fragments arranged by size: 2823 1071 921 328 300 237 158 85 BsmI GAATG_Cn' Cuts at: 3434 3527 3434 Size: 93 5830 BsmBI CGTCTCn'nnnn_ Cuts at: 2114 2114 Size: 5923 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2030 2040 2277 2435 2660 2696 2702 Size: 399 240 10 237 158 225 36 6 Cuts at: 2702 2763 3050 3063 3242 3984 4069 5140 1391 Size: 61 287 13 179 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 287 240 237 225 179 158 85 61 36 13 10 6 BspEI T'CCGG_A Cuts at: 5586 5586 Size: 5923 BspHI T'CATG_A Cuts at: 3812 5542 3812 Size: 1730 4193 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2208 3278 844 Size: 1364 1070 3489 Fragments arranged by size: 3489 1364 1070 BsrBI CCG'CTC Cuts at: 1394 1823 3810 1394 Size: 429 1987 3507 Fragments arranged by size: 3507 1987 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4418 4592 65 Size: 1183 3170 174 1396 Fragments arranged by size: 3170 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2165 4567 4754 1700 Size: 110 355 2402 187 2869 Fragments arranged by size: 2869 2402 355 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5923 BssHII G'CGCG_C Cuts at: 1925 2392 1925 Size: 467 5456 BssSI C'ACGA_G Cuts at: 3983 4995 3983 Size: 1012 4911 BstAPI GCAn_nnn'nTGC Cuts at: 794 2307 794 Size: 1513 4410 BstBI TT'CG_AA Cuts at: 4727 4727 Size: 5923 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5923 BstXI CCAn_nnnn'nTGG Cuts at: 2629 2629 Size: 5923 BstYI r'GATC_y Cuts at: 1972 2181 2710 3253 3620 4005 4022 5474 5560 Size: 209 529 543 367 385 17 1452 86 Cuts at: 5560 5572 5918 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 543 529 385 367 346 209 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2107 2457 2583 513 Size: 552 1042 350 126 3853 Fragments arranged by size: 3853 1042 552 350 126 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5184 Fragments arranged by size: 5184 683 56 BtsI GCAGTG_nn' Cuts at: 2149 2225 3439 4233 4253 2149 Size: 76 1214 794 20 3819 Fragments arranged by size: 3819 1214 794 76 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5923 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5888 DraI TTT'AAA Cuts at: 3227 3227 Size: 5923 DraIII CAC_nnn'GTG Cuts at: 2439 2439 Size: 5923 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2131 2489 2848 4930 809 Size: 507 815 358 359 2082 1802 Fragments arranged by size: 2082 1802 815 507 359 358 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2571 2589 2675 Size: 54 1425 50 275 18 741 18 86 Cuts at: 2675 3370 4259 5888 8 Size: 695 889 1629 43 Fragments arranged by size: 1629 1425 889 741 695 275 86 54 50 43 18 18 EagI C'GGCC_G Cuts at: 1537 1812 2571 2675 3370 1537 Size: 275 759 104 695 4090 Fragments arranged by size: 4090 759 695 275 104 EarI CTCTTCn'nnn_ Cuts at: 1353 3853 1353 Size: 2500 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2086 4510 4884 5030 387 Size: 1064 138 497 2424 374 146 1280 Fragments arranged by size: 2424 1280 1064 497 374 146 138 EcoICRI GAG'CTC Cuts at: 2275 3061 2275 Size: 786 5137 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2285 2918 3070 3191 3986 4583 5370 Size: 393 653 633 152 121 795 597 787 Cuts at: 5370 5638 1239 Size: 268 1524 Fragments arranged by size: 1524 795 787 653 633 597 393 268 152 121 EcoO109I rG'GnC_Cy Cuts at: 2027 2699 2929 2027 Size: 672 230 5021 Fragments arranged by size: 5021 672 230 EcoRI G'AATT_C Cuts at: 3232 3610 3232 Size: 378 5545 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2872 2872 Size: 5923 FspI TGC'GCA Cuts at: 2813 4429 2813 Size: 1616 4307 FspAI rTGC'GCAy Cuts at: 2813 2813 Size: 5923 HaeII r_GCGC'y Cuts at: 1678 2332 2347 2519 3026 5070 1678 Size: 654 15 172 507 2044 2531 Fragments arranged by size: 2531 2044 654 507 172 15 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2380 2412 4564 4596 4638 Size: 32 135 32 808 32 2152 32 42 Cuts at: 4638 4670 1373 Size: 32 2626 Fragments arranged by size: 2626 2152 808 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3012 3267 3513 669 Size: 988 281 1074 255 246 3079 Fragments arranged by size: 3079 1074 988 281 255 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5923 HpaI GTT'AAC Cuts at: 3513 3513 Size: 5923 KpnI G_GTAC'C Cuts at: 3248 3248 Size: 5923 MfeI C'AATT_G Cuts at: 3522 3522 Size: 5923 MluI A'CGCG_T Cuts at: 5799 5799 Size: 5923 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2250 3090 5037 5221 1305 Size: 945 840 1947 184 2007 Fragments arranged by size: 2007 1947 945 840 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4101 Fragments arranged by size: 4101 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2999 3881 4240 4399 518 Size: 648 1833 882 359 159 2042 Fragments arranged by size: 2042 1833 882 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2109 2355 2567 2576 2585 3339 4016 5164 Size: 503 246 212 9 9 754 677 1148 Cuts at: 5164 5409 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 754 677 503 246 245 212 9 9 NaeI GCC'GGC Cuts at: 2167 2167 Size: 5923 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5371 NdeI CA'TA_TG Cuts at: 387 387 Size: 5923 NgoMIV G'CCGG_C Cuts at: 2165 2165 Size: 5923 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5923 NotI GC'GGCC_GC Cuts at: 2571 3370 2571 Size: 799 5124 NspI r_CATG'y Cuts at: 1132 1229 2230 2812 3281 4826 1132 Size: 97 1001 582 469 1545 2229 Fragments arranged by size: 2229 1545 1001 582 469 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5923 PciI A'CATG_T Cuts at: 4822 4822 Size: 5923 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5923 PmeI GTTT'AAAC Cuts at: 3227 3227 Size: 5923 PmlI CAC'GTG Cuts at: 2430 2439 2430 Size: 9 5914 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3992 4024 5505 5537 3992 Size: 32 1481 32 4378 Fragments arranged by size: 4378 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2929 2929 Size: 5923 PshAI GACnn'nnGTC Cuts at: 1613 3143 1613 Size: 1530 4393 PsiI TTA'TAA Cuts at: 3493 3493 Size: 5923 PspOMI G'GGCC_C Cuts at: 2026 2698 3046 2026 Size: 672 348 4903 Fragments arranged by size: 4903 672 348 PstI C_TGCA'G Cuts at: 830 2526 2581 3030 3275 830 Size: 1696 55 449 245 3478 Fragments arranged by size: 3478 1696 449 245 55 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5923 RsrII CG'GwC_CG Cuts at: 2188 2188 Size: 5923 SacI G_AGCT'C Cuts at: 2277 3063 2277 Size: 786 5137 SacII CC_GC'GG Cuts at: 2110 2586 2110 Size: 476 5447 SalI G'TCGA_C Cuts at: 1936 3010 3265 1936 Size: 1074 255 4594 Fragments arranged by size: 4594 1074 255 SbfI CC_TGCA'GG Cuts at: 3275 3275 Size: 5923 ScaI AGT'ACT Cuts at: 4171 5883 4171 Size: 1712 4211 SfcI C'TryA_G Cuts at: 826 1045 2323 2522 2577 3026 3271 4406 5087 Size: 219 1278 199 55 449 245 1135 681 Cuts at: 5087 5278 826 Size: 191 1471 Fragments arranged by size: 1471 1278 1135 681 449 245 219 199 191 55 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5923 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5923 SmaI CCC'GGG Cuts at: 1872 2697 3250 1872 Size: 825 553 4545 Fragments arranged by size: 4545 825 553 SmlI C'TyrA_G Cuts at: 820 1017 1943 2237 4027 4928 5190 820 Size: 197 926 294 1790 901 262 1553 Fragments arranged by size: 1790 1553 926 901 294 262 197 SnaBI TAC'GTA Cuts at: 493 3714 493 Size: 3221 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5923 SphI G_CATG'C Cuts at: 1132 2812 3281 1132 Size: 1680 469 3774 Fragments arranged by size: 3774 1680 469 SrfI GCCC'GGGC Cuts at: 2697 2697 Size: 5923 SspI AAT'ATT Cuts at: 5 52 3847 5 Size: 47 3795 2081 Fragments arranged by size: 3795 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5923 StyI C'CwwG_G Cuts at: 513 1065 2883 3362 513 Size: 552 1818 479 3074 Fragments arranged by size: 3074 1818 552 479 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3950 3967 4120 4305 4750 325 Size: 1255 2370 17 153 185 445 1498 Fragments arranged by size: 2370 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4169 5881 96 Size: 274 80 33 51 1443 2192 1712 138 Fragments arranged by size: 2192 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2204 2236 5883 5915 2204 Size: 32 3647 32 2212 Fragments arranged by size: 3647 2212 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5923 XbaI T'CTAG_A Cuts at: 3259 3259 Size: 5923 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5923 XmaI C'CCGG_G Cuts at: 1870 2695 3248 1870 Size: 825 553 4545 Fragments arranged by size: 4545 825 553 XmnI GAAnn'nnTTC Cuts at: 4052 4052 Size: 5923 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3141 3731 276 Size: 53 83 186 936 1607 590 2468 Fragments arranged by size: 2468 1607 936 590 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI RsrII SacI SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BstZ17I Bsu36I EcoNI EcoRV FseI KasI NarI NruI NsiI PacI PfoI PspXI PsrI PvuII SanDI SapI SexAI SfiI SfoI SwaI XcmI