(Circular) (Six-base) MAPSORT of: pFN21AB7590.seq Check: 1036 from: 1 to: 5575 pFN21AB7590 5575 bp With 182 enzymes: SgfI * April 15, 2010 09:33 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3385 278 Size: 53 83 186 936 1849 2468 Fragments arranged by size: 2468 1849 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2918 1937 Size: 981 4594 Acc65I G'GTAC_C Cuts at: 2896 2896 Size: 5575 AclI AA'CG_TT Cuts at: 1563 3702 4075 1563 Size: 2139 373 3063 Fragments arranged by size: 3063 2139 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3638 5022 5290 1632 Size: 2006 1384 268 1917 Fragments arranged by size: 2006 1917 1384 268 AfeI AGC'GCT Cuts at: 2372 2372 Size: 5575 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5378 AflIII A'CryG_T Cuts at: 1203 2318 4474 5451 1203 Size: 1115 2156 977 1327 Fragments arranged by size: 2156 1327 1115 977 AgeI A'CCGG_T Cuts at: 4406 4406 Size: 5575 AhdI GACnn_n'nnGTC Cuts at: 4304 4304 Size: 5575 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5575 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2245 2261 1184 Size: 1061 16 4498 Fragments arranged by size: 4498 1061 16 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2348 2380 2348 Size: 32 5543 AlwNI CAG_nnn'CTG Cuts at: 2856 3318 4890 2856 Size: 462 1572 3541 Fragments arranged by size: 3541 1572 462 ApaI G_GGCC'C Cuts at: 2039 2214 2596 2039 Size: 175 382 5018 Fragments arranged by size: 5018 382 175 ApaLI G'TGCA_C Cuts at: 3632 4788 3632 Size: 1156 4419 ApoI r'AATT_y Cuts at: 1493 2884 3110 3262 5371 1493 Size: 1391 226 152 2109 1697 Fragments arranged by size: 2109 1697 1391 226 152 AseI AT'TA_AT Cuts at: 160 1032 4129 160 Size: 872 3097 1606 Fragments arranged by size: 3097 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5575 AvaI C'yCGr_G Cuts at: 1870 1943 2207 2900 1870 Size: 73 264 693 4545 Fragments arranged by size: 4545 693 264 73 BamHI G'GATC_C Cuts at: 2905 3272 2905 Size: 367 5208 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2018 2651 2896 4351 618 Size: 325 217 627 231 633 245 1455 1842 Fragments arranged by size: 1842 1455 633 627 325 245 231 217 BanII G_rGCy'C Cuts at: 1391 2039 2214 2596 2894 1391 Size: 648 175 382 298 4072 Fragments arranged by size: 4072 648 382 298 175 BbeI G_GCGC'C Cuts at: 2022 2022 Size: 5575 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4604 BbvCI CC'TCA_GC Cuts at: 2152 2518 2710 2152 Size: 366 192 5017 Fragments arranged by size: 5017 366 192 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2516 2550 3766 3800 1903 Size: 34 579 34 1216 34 3678 Fragments arranged by size: 3678 1216 579 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3467 4677 3467 Size: 1210 4365 BclI T'GATC_A Cuts at: 1548 1548 Size: 5575 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2528 2628 2649 Size: 107 122 71 631 605 856 100 21 Cuts at: 2649 4186 136 Size: 1537 1525 Fragments arranged by size: 1537 1525 856 631 605 122 107 100 71 21 BglII A'GATC_T Cuts at: 5570 5570 Size: 5575 BlpI GC'TnA_GC Cuts at: 2992 2992 Size: 5575 Bme1580I G_kGCm'C Cuts at: 1790 2039 2214 2589 2596 2654 3636 4792 1790 Size: 249 175 375 7 58 982 1156 2573 Fragments arranged by size: 2573 1156 982 375 249 175 58 7 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5575 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2804 3306 4264 Size: 416 53 83 399 24 1387 502 958 Cuts at: 4264 442 Size: 1753 Fragments arranged by size: 1753 1387 958 502 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5575 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2143 2158 2375 2519 2582 4235 1239 Size: 904 15 217 144 63 1653 2579 Fragments arranged by size: 2579 1653 904 217 144 63 15 Bpu10I CC'TnA_GC Cuts at: 2152 2518 2710 2152 Size: 366 192 5017 Fragments arranged by size: 5017 366 192 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3700 4565 4863 3700 Size: 865 298 4412 Fragments arranged by size: 4412 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2076 2426 2487 4238 882 Size: 617 577 350 61 1751 2219 Fragments arranged by size: 2219 1751 617 577 350 61 BsaAI yAC'GTr Cuts at: 493 1204 2321 3366 493 Size: 711 1117 1045 2702 Fragments arranged by size: 2702 1117 1045 711 BsaBI GATnn'nnATC Cuts at: 5569 5569 Size: 5575 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2019 2510 3382 3764 Size: 53 83 186 936 486 491 872 382 Cuts at: 3764 4399 275 Size: 635 1451 Fragments arranged by size: 1451 936 872 635 491 486 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4008 4406 4680 4827 5238 4008 Size: 398 274 147 411 4345 Fragments arranged by size: 4345 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2348 2378 1178 Size: 30 165 30 945 30 4375 Fragments arranged by size: 4375 945 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2506 2668 1186 Size: 1320 162 4093 Fragments arranged by size: 4093 1320 162 BseYI C'CCAG_C Cuts at: 4778 5274 4778 Size: 496 5079 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5002 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2032 3025 3786 4814 1540 Size: 275 186 31 993 761 1028 2301 Fragments arranged by size: 2301 1028 993 761 275 186 31 BsiHKAI G_wGCw'C Cuts at: 3636 3721 4792 3636 Size: 85 1071 4419 Fragments arranged by size: 4419 1071 85 BsmI GAATG_Cn' Cuts at: 3086 3179 3086 Size: 93 5482 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2039 2214 2589 2596 2654 2894 3636 Size: 399 249 175 375 7 58 240 742 Cuts at: 3636 3721 4792 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 375 249 240 175 85 58 7 BspEI T'CCGG_A Cuts at: 5238 5238 Size: 5575 BspHI T'CATG_A Cuts at: 3464 5194 3464 Size: 1730 3845 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2644 2930 844 Size: 1800 286 3489 Fragments arranged by size: 3489 1800 286 BsrBI CCG'CTC Cuts at: 1394 1823 2096 2767 3462 1394 Size: 429 273 671 695 3507 Fragments arranged by size: 3507 695 671 429 273 BsrDI GCAATG_nn' Cuts at: 65 1248 4070 4244 65 Size: 1183 2822 174 1396 Fragments arranged by size: 2822 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2304 2571 2783 4219 4406 1700 Size: 110 494 267 212 1436 187 2869 Fragments arranged by size: 2869 1436 494 267 212 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5575 BssHII G'CGCG_C Cuts at: 1925 2042 1925 Size: 117 5458 BssSI C'ACGA_G Cuts at: 3635 4647 3635 Size: 1012 4563 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5575 BstBI TT'CG_AA Cuts at: 4379 4379 Size: 5575 BstEII G'GTnAC_C Cuts at: 1184 2663 1184 Size: 1479 4096 BstXI CCAn_nnnn'nTGG Cuts at: 2823 2823 Size: 5575 BstYI r'GATC_y Cuts at: 1972 2773 2905 3272 3657 3674 5126 5212 5224 Size: 801 132 367 385 17 1452 86 12 Cuts at: 5224 5570 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 801 385 367 346 132 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2286 2659 2286 Size: 373 5202 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4084 Fragments arranged by size: 4084 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2025 487 Size: 683 56 799 4037 Fragments arranged by size: 4037 799 683 56 BtsI GCAGTG_nn' Cuts at: 2227 2331 2723 3091 3885 3905 2227 Size: 104 392 368 794 20 3897 Fragments arranged by size: 3897 794 392 368 104 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5575 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2121 2156 529 Size: 35 1557 35 3948 Fragments arranged by size: 3948 1557 35 35 DraI TTT'AAA Cuts at: 2879 2879 Size: 5575 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4582 809 Size: 507 3266 1802 Fragments arranged by size: 3266 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2122 2142 3022 Size: 54 1425 50 275 18 292 20 880 Cuts at: 3022 3911 5540 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 880 292 275 54 50 43 20 18 EagI C'GGCC_G Cuts at: 1537 1812 3022 1537 Size: 275 1210 4090 Fragments arranged by size: 4090 1210 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3505 1353 Size: 2152 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2066 4162 4536 4682 387 Size: 1064 138 477 2096 374 146 1280 Fragments arranged by size: 2096 1280 1064 477 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2143 2158 2375 2519 2582 3638 4235 Size: 393 511 15 217 144 63 1056 597 Cuts at: 4235 5022 5290 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1056 787 597 511 393 268 217 144 63 15 EcoNI CCTnn'n_nnAGG Cuts at: 2285 2285 Size: 5575 EcoO109I rG'GnC_Cy Cuts at: 2035 2036 2148 2290 2592 2593 2035 Size: 1 112 142 302 1 5017 Fragments arranged by size: 5017 302 142 112 1 1 EcoRI G'AATT_C Cuts at: 2884 3262 2884 Size: 378 5197 FspI TGC'GCA Cuts at: 4081 4081 Size: 5575 HaeII r_GCGC'y Cuts at: 1678 2022 2077 2374 2612 2850 4722 1678 Size: 344 55 297 238 238 1872 2531 Fragments arranged by size: 2531 1872 344 297 238 238 55 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4216 4248 4290 4322 1373 Size: 32 135 32 2644 32 42 32 2626 Fragments arranged by size: 2644 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2919 3165 669 Size: 988 281 981 246 3079 Fragments arranged by size: 3079 988 981 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5575 HpaI GTT'AAC Cuts at: 3165 3165 Size: 5575 KasI G'GCGC_C Cuts at: 2018 2018 Size: 5575 KpnI G_GTAC'C Cuts at: 2900 2900 Size: 5575 MfeI C'AATT_G Cuts at: 3174 3174 Size: 5575 MluI A'CGCG_T Cuts at: 5451 5451 Size: 5575 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2568 4689 4873 1305 Size: 1263 2121 184 2007 Fragments arranged by size: 2121 2007 1263 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2124 10 Size: 54 1425 343 292 3461 Fragments arranged by size: 3461 1425 343 292 54 MslI CAynn'nnrTG Cuts at: 518 1166 2243 3533 3892 4051 518 Size: 648 1077 1290 359 159 2042 Fragments arranged by size: 2042 1290 1077 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2091 2344 2419 2698 2744 2823 2856 2991 Size: 485 253 75 279 46 79 33 135 Cuts at: 2991 3668 4816 5061 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 485 279 253 245 135 79 75 46 33 NaeI GCC'GGC Cuts at: 2573 2785 2573 Size: 212 5363 NarI GG'CG_CC Cuts at: 2019 2019 Size: 5575 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4084 Fragments arranged by size: 4084 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5575 NgoMIV G'CCGG_C Cuts at: 2571 2783 2571 Size: 212 5363 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5575 NotI GC'GGCC_GC Cuts at: 3022 3022 Size: 5575 NspI r_CATG'y Cuts at: 1132 1229 2933 4478 1132 Size: 97 1704 1545 2229 Fragments arranged by size: 2229 1704 1545 97 PasI CC'CwG_GG Cuts at: 1348 2589 1348 Size: 1241 4334 PciI A'CATG_T Cuts at: 4474 4474 Size: 5575 PflMI CCAn_nnn'nTGG Cuts at: 1108 2383 1108 Size: 1275 4300 PfoI T'CCnGG_A Cuts at: 2769 2769 Size: 5575 PmeI GTTT'AAAC Cuts at: 2879 2879 Size: 5575 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2348 2380 3644 3676 5157 5189 2348 Size: 32 1264 32 1481 32 2734 Fragments arranged by size: 2734 1481 1264 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2148 2148 Size: 5575 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5575 PsiI TTA'TAA Cuts at: 3145 3145 Size: 5575 PspOMI G'GGCC_C Cuts at: 2035 2210 2592 2035 Size: 175 382 5018 Fragments arranged by size: 5018 382 175 PstI C_TGCA'G Cuts at: 830 2927 830 Size: 2097 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5575 PvuII CAG'CTG Cuts at: 2344 2419 2744 2823 2856 2344 Size: 75 325 79 33 5063 Fragments arranged by size: 5063 325 79 75 33 SalI G'TCGA_C Cuts at: 1936 2917 1936 Size: 981 4594 SbfI CC_TGCA'GG Cuts at: 2927 2927 Size: 5575 ScaI AGT'ACT Cuts at: 3823 5535 3823 Size: 1712 3863 SfcI C'TryA_G Cuts at: 826 1045 2923 4058 4739 4930 826 Size: 219 1878 1135 681 191 1471 Fragments arranged by size: 1878 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2020 2020 Size: 5575 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5575 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5575 SmaI CCC'GGG Cuts at: 1872 2209 2902 1872 Size: 337 693 4545 Fragments arranged by size: 4545 693 337 SmlI C'TyrA_G Cuts at: 820 1017 1943 3679 4580 4842 820 Size: 197 926 1736 901 262 1553 Fragments arranged by size: 1736 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3366 493 Size: 2873 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5575 SphI G_CATG'C Cuts at: 1132 2933 1132 Size: 1801 3774 SrfI GCCC'GGGC Cuts at: 2209 2209 Size: 5575 SspI AAT'ATT Cuts at: 5 52 3499 5 Size: 47 3447 2081 Fragments arranged by size: 3447 2081 47 StuI AGG'CCT Cuts at: 1517 2531 2631 1517 Size: 1014 100 4461 Fragments arranged by size: 4461 1014 100 StyI C'CwwG_G Cuts at: 513 1065 2004 3014 513 Size: 552 939 1010 3074 Fragments arranged by size: 3074 1010 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3602 3619 3772 3957 4402 325 Size: 1255 2022 17 153 185 445 1498 Fragments arranged by size: 2022 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3821 5533 96 Size: 274 80 33 51 1443 1844 1712 138 Fragments arranged by size: 1844 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5535 5567 5535 Size: 32 5543 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5575 XbaI T'CTAG_A Cuts at: 2911 2911 Size: 5575 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5575 XmaI C'CCGG_G Cuts at: 1870 2207 2900 1870 Size: 337 693 4545 Fragments arranged by size: 4545 693 337 XmnI GAAnn'nnTTC Cuts at: 3704 3704 Size: 5575 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3383 276 Size: 53 83 186 936 1849 2468 Fragments arranged by size: 2468 1849 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SwaI XcmI