(Circular) (Six-base) MAPSORT of: pFN21AB9971.seq Check: 601 from: 1 to: 6367 pFN21AB9971 6367 bp With 182 enzymes: SgfI * April 15, 2010 09:33 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2358 2358 Size: 6367 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4177 278 Size: 53 83 186 936 2641 2468 Fragments arranged by size: 2641 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2313 3710 1937 Size: 376 1397 4594 Fragments arranged by size: 4594 1397 376 Acc65I G'GTAC_C Cuts at: 3688 3688 Size: 6367 AclI AA'CG_TT Cuts at: 1563 4494 4867 1563 Size: 2931 373 3063 Fragments arranged by size: 3063 2931 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2913 3442 4430 5814 6082 1632 Size: 1281 529 988 1384 268 1917 Fragments arranged by size: 1917 1384 1281 988 529 268 AflII C'TTAA_G Cuts at: 820 1017 3635 820 Size: 197 2618 3552 Fragments arranged by size: 3552 2618 197 AflIII A'CryG_T Cuts at: 1203 2601 5266 6243 1203 Size: 1398 2665 977 1327 Fragments arranged by size: 2665 1398 1327 977 AgeI A'CCGG_T Cuts at: 5198 5198 Size: 6367 AhdI GACnn_n'nnGTC Cuts at: 5096 5096 Size: 6367 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6367 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6367 AlwNI CAG_nnn'CTG Cuts at: 2635 4110 5682 2635 Size: 1475 1572 3320 Fragments arranged by size: 3320 1572 1475 ApaI G_GGCC'C Cuts at: 2241 2241 Size: 6367 ApaLI G'TGCA_C Cuts at: 4424 5580 4424 Size: 1156 5211 ApoI r'AATT_y Cuts at: 1493 2681 3232 3676 3902 4054 6163 1493 Size: 1188 551 444 226 152 2109 1697 Fragments arranged by size: 2109 1697 1188 551 444 226 152 AseI AT'TA_AT Cuts at: 160 1032 4921 160 Size: 872 3889 1606 Fragments arranged by size: 3889 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6367 AvaI C'yCGr_G Cuts at: 1870 1943 2692 3166 3658 3692 1870 Size: 73 749 474 492 34 4545 Fragments arranged by size: 4545 749 492 474 73 34 BamHI G'GATC_C Cuts at: 3697 4064 3697 Size: 367 6000 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3110 3662 3688 5143 618 Size: 325 217 627 1323 552 26 1455 1842 Fragments arranged by size: 1842 1455 1323 627 552 325 217 26 BanII G_rGCy'C Cuts at: 1391 2201 2241 2699 2999 3096 3686 1391 Size: 810 40 458 300 97 590 4072 Fragments arranged by size: 4072 810 590 458 300 97 40 BbeI G_GCGC'C Cuts at: 3114 3114 Size: 6367 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2116 3448 928 Size: 971 217 1332 3847 Fragments arranged by size: 3847 1332 971 217 BbvCI CC'TCA_GC Cuts at: 2303 3464 2303 Size: 1161 5206 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4558 4592 1903 Size: 34 50 34 2537 34 3678 Fragments arranged by size: 3678 2537 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4259 5469 4259 Size: 1210 5157 BclI T'GATC_A Cuts at: 1548 1548 Size: 6367 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2373 2785 4978 Size: 107 122 71 631 605 701 412 2193 Cuts at: 4978 136 Size: 1525 Fragments arranged by size: 2193 1525 701 631 605 412 122 107 71 BglII A'GATC_T Cuts at: 6362 6362 Size: 6367 BlpI GC'TnA_GC Cuts at: 2172 2177 2993 3366 3784 2172 Size: 5 816 373 418 4755 Fragments arranged by size: 4755 816 418 373 5 Bme1580I G_kGCm'C Cuts at: 1790 2241 3665 4428 5584 1790 Size: 451 1424 763 1156 2573 Fragments arranged by size: 2573 1424 1156 763 451 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6367 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4098 5056 442 Size: 416 53 83 399 24 2681 958 1753 Fragments arranged by size: 2681 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2328 1056 Size: 1272 5095 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2048 2215 2683 5027 1239 Size: 809 167 468 2344 2579 Fragments arranged by size: 2579 2344 809 468 167 Bpu10I CC'TnA_GC Cuts at: 2303 3464 2303 Size: 1161 5206 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4492 5357 5655 4492 Size: 865 298 5204 Fragments arranged by size: 5204 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3542 3566 5030 882 Size: 617 2043 24 1464 2219 Fragments arranged by size: 2219 2043 1464 617 24 BsaAI yAC'GTr Cuts at: 493 1204 4158 493 Size: 711 2954 2702 Fragments arranged by size: 2954 2702 711 BsaBI GATnn'nnATC Cuts at: 3269 6361 3269 Size: 3092 3275 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3111 3585 4174 4556 Size: 53 83 186 936 1578 474 589 382 Cuts at: 4556 5191 275 Size: 635 1451 Fragments arranged by size: 1578 1451 936 635 589 474 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4800 5198 5472 5619 6030 4800 Size: 398 274 147 411 5137 Fragments arranged by size: 5137 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3390 3420 1178 Size: 30 165 30 1987 30 4125 Fragments arranged by size: 4125 1987 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2222 1186 Size: 1036 5331 BseYI C'CCAG_C Cuts at: 2356 2376 5570 6066 2356 Size: 20 3194 496 2657 Fragments arranged by size: 3194 2657 496 20 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2287 3086 3269 1163 Size: 573 551 799 183 4261 Fragments arranged by size: 4261 799 573 551 183 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3817 4578 5606 1540 Size: 275 186 1816 761 1028 2301 Fragments arranged by size: 2301 1816 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2580 3084 4428 4513 5584 2580 Size: 504 1344 85 1071 3363 Fragments arranged by size: 3363 1344 1071 504 85 BsmI GAATG_Cn' Cuts at: 2476 3367 3878 3971 2476 Size: 891 511 93 4872 Fragments arranged by size: 4872 891 511 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2201 2241 2580 2699 2999 3084 3096 Size: 399 411 40 339 119 300 85 12 Cuts at: 3096 3665 3686 4428 4513 5584 1391 Size: 569 21 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 569 411 399 339 300 119 85 85 40 21 12 BspEI T'CCGG_A Cuts at: 6030 6030 Size: 6367 BspHI T'CATG_A Cuts at: 3261 4256 5986 3261 Size: 995 1730 3642 Fragments arranged by size: 3642 1730 995 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2023 2358 3291 3722 844 Size: 1179 335 933 431 3489 Fragments arranged by size: 3489 1179 933 431 335 BsrBI CCG'CTC Cuts at: 1394 1823 3152 4254 1394 Size: 429 1329 1102 3507 Fragments arranged by size: 3507 1329 1102 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3333 4862 5036 65 Size: 1183 2085 1529 174 1396 Fragments arranged by size: 2085 1529 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5011 5198 1700 Size: 110 3201 187 2869 Fragments arranged by size: 3201 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6367 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6367 BssSI C'ACGA_G Cuts at: 4427 5439 4427 Size: 1012 5355 BstAPI GCAn_nnn'nTGC Cuts at: 794 3484 794 Size: 2690 3677 BstBI TT'CG_AA Cuts at: 5171 5171 Size: 6367 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6367 BstXI CCAn_nnnn'nTGG Cuts at: 2113 2162 2189 2113 Size: 49 27 6291 Fragments arranged by size: 6291 49 27 BstYI r'GATC_y Cuts at: 1972 3697 4064 4449 4466 5918 6004 6016 6362 Size: 1725 367 385 17 1452 86 12 346 Cuts at: 6362 1972 Size: 1977 Fragments arranged by size: 1977 1725 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3032 3032 Size: 6367 BtgI C'CryG_G Cuts at: 513 1065 2013 2241 3219 3522 513 Size: 552 948 228 978 303 3358 Fragments arranged by size: 3358 978 948 552 303 228 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2902 487 Size: 683 56 1676 3952 Fragments arranged by size: 3952 1676 683 56 BtsI GCAGTG_nn' Cuts at: 2149 3883 4677 4697 2149 Size: 1734 794 20 3819 Fragments arranged by size: 3819 1734 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6367 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6332 DraI TTT'AAA Cuts at: 2955 3671 2955 Size: 716 5651 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5374 809 Size: 507 4058 1802 Fragments arranged by size: 4058 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2260 2331 2524 Size: 54 1425 50 275 18 430 71 193 Cuts at: 2524 3222 3316 3814 4703 6332 8 Size: 698 94 498 889 1629 43 Fragments arranged by size: 1629 1425 889 698 498 430 275 193 94 71 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3814 1537 Size: 275 2002 4090 Fragments arranged by size: 4090 2002 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2404 2621 2831 2967 4297 1353 Size: 1051 217 210 136 1330 3423 Fragments arranged by size: 3423 1330 1051 217 210 136 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2609 4954 5328 5474 387 Size: 1064 138 1020 2345 374 146 1280 Fragments arranged by size: 2345 1280 1064 1020 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2048 2215 2683 2913 3442 4430 5027 Size: 393 416 167 468 230 529 988 597 Cuts at: 5027 5814 6082 1239 Size: 787 268 1524 Fragments arranged by size: 1524 988 787 597 529 468 416 393 268 230 167 EcoNI CCTnn'n_nnAGG Cuts at: 2627 2972 2627 Size: 345 6022 EcoO109I rG'GnC_Cy Cuts at: 2237 2360 2632 3171 2237 Size: 123 272 539 5433 Fragments arranged by size: 5433 539 272 123 EcoRI G'AATT_C Cuts at: 3676 4054 3676 Size: 378 5989 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2083 3061 2083 Size: 978 5389 FspI TGC'GCA Cuts at: 4873 4873 Size: 6367 HaeII r_GCGC'y Cuts at: 1678 2275 2301 2756 2791 3114 5514 1678 Size: 597 26 455 35 323 2400 2531 Fragments arranged by size: 2531 2400 597 455 323 35 26 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 5008 5040 5082 5114 1373 Size: 32 135 32 3436 32 42 32 2626 Fragments arranged by size: 3436 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2560 3711 3957 669 Size: 988 281 622 1151 246 3079 Fragments arranged by size: 3079 1151 988 622 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6367 HpaI GTT'AAC Cuts at: 3957 3957 Size: 6367 KasI G'GCGC_C Cuts at: 3110 3110 Size: 6367 KpnI G_GTAC'C Cuts at: 3692 3692 Size: 6367 MfeI C'AATT_G Cuts at: 3966 3966 Size: 6367 MluI A'CGCG_T Cuts at: 6243 6243 Size: 6367 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5481 5665 1305 Size: 4176 184 2007 Fragments arranged by size: 4176 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2262 2333 3224 10 Size: 54 1425 343 430 71 891 3153 Fragments arranged by size: 3153 1425 891 430 343 71 54 MslI CAynn'nnrTG Cuts at: 518 1166 2111 2606 3053 3224 3305 4325 4684 Size: 648 945 495 447 171 81 1020 359 Cuts at: 4684 4843 518 Size: 159 2042 Fragments arranged by size: 2042 1020 945 648 495 447 359 171 159 81 MspA1I CmG'CkG Cuts at: 1606 2243 2307 2356 2800 3120 3524 3783 4460 Size: 637 64 49 444 320 404 259 677 Cuts at: 4460 5608 5853 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 677 637 444 404 320 259 245 64 49 NarI GG'CG_CC Cuts at: 3111 3111 Size: 6367 NcoI C'CATG_G Cuts at: 513 1065 3219 513 Size: 552 2154 3661 Fragments arranged by size: 3661 2154 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6367 NheI G'CTAG_C Cuts at: 1052 2324 1052 Size: 1272 5095 NotI GC'GGCC_GC Cuts at: 3814 3814 Size: 6367 NspI r_CATG'y Cuts at: 1132 1229 2605 3725 5270 1132 Size: 97 1376 1120 1545 2229 Fragments arranged by size: 2229 1545 1376 1120 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6367 PciI A'CATG_T Cuts at: 2601 5266 2601 Size: 2665 3702 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6367 PfoI T'CCnGG_A Cuts at: 2438 2438 Size: 6367 PmeI GTTT'AAAC Cuts at: 3671 3671 Size: 6367 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4436 4468 5949 5981 4436 Size: 32 1481 32 4822 Fragments arranged by size: 4822 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2360 2360 Size: 6367 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6367 PsiI TTA'TAA Cuts at: 3937 3937 Size: 6367 PspOMI G'GGCC_C Cuts at: 2237 2237 Size: 6367 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3050 3082 3050 Size: 32 6335 PstI C_TGCA'G Cuts at: 830 2631 2799 3719 830 Size: 1801 168 920 3478 Fragments arranged by size: 3478 1801 920 168 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6367 PvuII CAG'CTG Cuts at: 2307 2800 2307 Size: 493 5874 SacII CC_GC'GG Cuts at: 2244 3525 2244 Size: 1281 5086 SalI G'TCGA_C Cuts at: 1936 3709 1936 Size: 1773 4594 SanDI GG'GwC_CC Cuts at: 2360 2360 Size: 6367 SapI GCTCTTCn'nnn_ Cuts at: 2404 2621 2404 Size: 217 6150 SbfI CC_TGCA'GG Cuts at: 3719 3719 Size: 6367 ScaI AGT'ACT Cuts at: 4615 6327 4615 Size: 1712 4655 SexAI A'CCwGG_T Cuts at: 2421 2421 Size: 6367 SfcI C'TryA_G Cuts at: 826 1045 2627 2795 3715 4850 5531 5722 826 Size: 219 1582 168 920 1135 681 191 1471 Fragments arranged by size: 1582 1471 1135 920 681 219 191 168 SfoI GGC'GCC Cuts at: 3112 3112 Size: 6367 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6367 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6367 SmaI CCC'GGG Cuts at: 1872 3168 3694 1872 Size: 1296 526 4545 Fragments arranged by size: 4545 1296 526 SmlI C'TyrA_G Cuts at: 820 1017 1943 3635 4471 5372 5634 820 Size: 197 926 1692 836 901 262 1553 Fragments arranged by size: 1692 1553 926 901 836 262 197 SnaBI TAC'GTA Cuts at: 493 4158 493 Size: 3665 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6367 SphI G_CATG'C Cuts at: 1132 3725 1132 Size: 2593 3774 SspI AAT'ATT Cuts at: 5 52 4291 5 Size: 47 4239 2081 Fragments arranged by size: 4239 2081 47 StuI AGG'CCT Cuts at: 1517 2232 2252 1517 Size: 715 20 5632 Fragments arranged by size: 5632 715 20 StyI C'CwwG_G Cuts at: 513 1065 2257 3219 3552 3595 3806 513 Size: 552 1192 962 333 43 211 3074 Fragments arranged by size: 3074 1192 962 552 333 211 43 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2368 4394 4411 4564 4749 5194 325 Size: 1255 788 2026 17 153 185 445 1498 Fragments arranged by size: 2026 1498 1255 788 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4613 6325 96 Size: 274 80 33 51 1443 2636 1712 138 Fragments arranged by size: 2636 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3390 3422 6327 6359 3390 Size: 32 2905 32 3398 Fragments arranged by size: 3398 2905 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6367 XbaI T'CTAG_A Cuts at: 3703 3703 Size: 6367 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2707 3199 2011 Size: 696 492 5179 Fragments arranged by size: 5179 696 492 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6367 XmaI C'CCGG_G Cuts at: 1870 3166 3692 1870 Size: 1296 526 4545 Fragments arranged by size: 4545 1296 526 XmnI GAAnn'nnTTC Cuts at: 4496 4496 Size: 6367 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4175 276 Size: 53 83 186 936 2641 2468 Fragments arranged by size: 2641 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspXI RsrII SacI SfiI SrfI SwaI