(Circular) (Six-base) MAPSORT of: pFN21AB6940.seq Check: 9499 from: 1 to: 5392 pFN21AB6940 5392 bp With 182 enzymes: SgfI * April 15, 2010 09:33 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2339 2339 Size: 5392 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3202 278 Size: 53 83 186 936 1666 2468 Fragments arranged by size: 2468 1666 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2735 1937 Size: 798 4594 Acc65I G'GTAC_C Cuts at: 2713 2713 Size: 5392 AclI AA'CG_TT Cuts at: 1563 3519 3892 1563 Size: 1956 373 3063 Fragments arranged by size: 3063 1956 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3455 4839 5107 1632 Size: 1823 1384 268 1917 Fragments arranged by size: 1917 1823 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5195 AflIII A'CryG_T Cuts at: 1203 2242 2574 4291 5268 1203 Size: 1039 332 1717 977 1327 Fragments arranged by size: 1717 1327 1039 977 332 AgeI A'CCGG_T Cuts at: 4223 4223 Size: 5392 AhdI GACnn_n'nnGTC Cuts at: 4121 4121 Size: 5392 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5392 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2635 2645 1184 Size: 1451 10 3931 Fragments arranged by size: 3931 1451 10 AlwNI CAG_nnn'CTG Cuts at: 2521 3135 4707 2521 Size: 614 1572 3206 Fragments arranged by size: 3206 1572 614 ApaLI G'TGCA_C Cuts at: 2543 3449 4605 2543 Size: 906 1156 3330 Fragments arranged by size: 3330 1156 906 ApoI r'AATT_y Cuts at: 1493 2212 2701 2927 3079 5188 1493 Size: 719 489 226 152 2109 1697 Fragments arranged by size: 2109 1697 719 489 226 152 AseI AT'TA_AT Cuts at: 160 1032 3946 160 Size: 872 2914 1606 Fragments arranged by size: 2914 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5392 AvaI C'yCGr_G Cuts at: 1870 1943 2427 2717 1870 Size: 73 484 290 4545 Fragments arranged by size: 4545 484 290 73 BamHI G'GATC_C Cuts at: 2722 3089 2722 Size: 367 5025 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2555 2713 4168 618 Size: 325 217 627 768 158 1455 1842 Fragments arranged by size: 1842 1455 768 627 325 217 158 BanII G_rGCy'C Cuts at: 1391 2711 1391 Size: 1320 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4421 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3583 3617 1903 Size: 34 1646 34 3678 Fragments arranged by size: 3678 1646 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2518 3284 4494 2518 Size: 766 1210 3416 Fragments arranged by size: 3416 1210 766 BclI T'GATC_A Cuts at: 1548 2640 1548 Size: 1092 4300 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4003 136 Size: 107 122 71 631 605 2331 1525 Fragments arranged by size: 2331 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2616 5387 2616 Size: 2771 2621 BlpI GC'TnA_GC Cuts at: 2809 2809 Size: 5392 Bme1580I G_kGCm'C Cuts at: 1790 2547 3453 4609 1790 Size: 757 906 1156 2573 Fragments arranged by size: 2573 1156 906 757 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5392 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3123 4081 442 Size: 416 53 83 399 24 1706 958 1753 Fragments arranged by size: 1753 1706 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5392 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2294 4052 1239 Size: 1055 1758 2579 Fragments arranged by size: 2579 1758 1055 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2468 3517 4382 4680 2468 Size: 1049 865 298 3180 Fragments arranged by size: 3180 1049 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4055 882 Size: 617 2556 2219 Fragments arranged by size: 2556 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3183 493 Size: 711 1979 2702 Fragments arranged by size: 2702 1979 711 BsaBI GATnn'nnATC Cuts at: 5386 5386 Size: 5392 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3199 3581 4216 275 Size: 53 83 186 936 1666 382 635 1451 Fragments arranged by size: 1666 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3825 4223 4497 4644 5055 3825 Size: 398 274 147 411 4162 Fragments arranged by size: 4162 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5167 Fragments arranged by size: 5167 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2471 2684 1186 Size: 1285 213 3894 Fragments arranged by size: 3894 1285 213 BseYI C'CCAG_C Cuts at: 2026 4595 5091 2026 Size: 2569 496 2327 Fragments arranged by size: 2569 2327 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2174 2534 1163 Size: 573 438 360 4021 Fragments arranged by size: 4021 573 438 360 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2842 3603 4631 1540 Size: 275 186 841 761 1028 2301 Fragments arranged by size: 2301 1028 841 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2547 3453 3538 4609 2547 Size: 906 85 1071 3330 Fragments arranged by size: 3330 1071 906 85 BsmI GAATG_Cn' Cuts at: 2903 2996 2903 Size: 93 5299 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2547 2711 3453 3538 4609 1391 Size: 399 757 164 742 85 1071 2174 Fragments arranged by size: 2174 1071 757 742 399 164 85 BspEI T'CCGG_A Cuts at: 5055 5055 Size: 5392 BspHI T'CATG_A Cuts at: 3281 5011 3281 Size: 1730 3662 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2339 2747 844 Size: 1495 408 3489 Fragments arranged by size: 3489 1495 408 BsrBI CCG'CTC Cuts at: 1394 1823 3279 1394 Size: 429 1456 3507 Fragments arranged by size: 3507 1456 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2685 3887 4061 65 Size: 1183 1437 1202 174 1396 Fragments arranged by size: 1437 1396 1202 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4036 4223 1700 Size: 110 2226 187 2869 Fragments arranged by size: 2869 2226 187 110 BsrGI T'GTAC_A Cuts at: 96 2368 96 Size: 2272 3120 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5392 BssSI C'ACGA_G Cuts at: 3452 4464 3452 Size: 1012 4380 BstAPI GCAn_nnn'nTGC Cuts at: 794 2161 794 Size: 1367 4025 BstBI TT'CG_AA Cuts at: 4196 4196 Size: 5392 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5392 BstYI r'GATC_y Cuts at: 1972 2616 2722 3089 3474 3491 4943 5029 5041 Size: 644 106 367 385 17 1452 86 12 Cuts at: 5041 5387 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 644 385 367 346 106 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2289 2667 2289 Size: 378 5014 BtgI C'CryG_G Cuts at: 513 1065 2148 513 Size: 552 1083 3757 Fragments arranged by size: 3757 1083 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4653 Fragments arranged by size: 4653 683 56 BtsI GCAGTG_nn' Cuts at: 2908 3702 3722 2908 Size: 794 20 4578 Fragments arranged by size: 4578 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5392 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5357 DraI TTT'AAA Cuts at: 2696 2696 Size: 5392 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4399 809 Size: 507 3083 1802 Fragments arranged by size: 3083 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2839 3728 5357 Size: 54 1425 50 275 18 1009 889 1629 Cuts at: 5357 8 Size: 43 Fragments arranged by size: 1629 1425 1009 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2839 1537 Size: 275 1027 4090 Fragments arranged by size: 4090 1027 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3322 1353 Size: 1969 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3979 4353 4499 387 Size: 1064 138 2390 374 146 1280 Fragments arranged by size: 2390 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2294 3455 4052 4839 5107 1239 Size: 393 662 1161 597 787 268 1524 Fragments arranged by size: 1524 1161 787 662 597 393 268 EcoRI G'AATT_C Cuts at: 2701 3079 2701 Size: 378 5014 FspI TGC'GCA Cuts at: 3898 3898 Size: 5392 HaeII r_GCGC'y Cuts at: 1678 4539 1678 Size: 2861 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4033 4065 4107 4139 1373 Size: 32 135 32 2461 32 42 32 2626 Fragments arranged by size: 2626 2461 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2736 2982 669 Size: 988 281 798 246 3079 Fragments arranged by size: 3079 988 798 281 246 HindIII A'AGCT_T Cuts at: 748 2501 748 Size: 1753 3639 HpaI GTT'AAC Cuts at: 2982 2982 Size: 5392 KpnI G_GTAC'C Cuts at: 2717 2717 Size: 5392 MfeI C'AATT_G Cuts at: 2991 2991 Size: 5392 MluI A'CGCG_T Cuts at: 5268 5268 Size: 5392 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2634 4506 4690 1305 Size: 1329 1872 184 2007 Fragments arranged by size: 2007 1872 1329 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3570 Fragments arranged by size: 3570 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3350 3709 3868 518 Size: 648 2184 359 159 2042 Fragments arranged by size: 2184 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2808 3485 4633 4878 1606 Size: 1202 677 1148 245 2120 Fragments arranged by size: 2120 1202 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2148 513 Size: 552 1083 3757 Fragments arranged by size: 3757 1083 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5392 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5392 NotI GC'GGCC_GC Cuts at: 2839 2839 Size: 5392 NspI r_CATG'y Cuts at: 1132 1229 2045 2246 2578 2750 4295 1132 Size: 97 816 201 332 172 1545 2229 Fragments arranged by size: 2229 1545 816 332 201 172 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5392 PciI A'CATG_T Cuts at: 2242 2574 4291 2242 Size: 332 1717 3343 Fragments arranged by size: 3343 1717 332 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5392 PfoI T'CCnGG_A Cuts at: 2520 2520 Size: 5392 PmeI GTTT'AAAC Cuts at: 2696 2696 Size: 5392 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3461 3493 4974 5006 3461 Size: 32 1481 32 3847 Fragments arranged by size: 3847 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5392 PsiI TTA'TAA Cuts at: 2962 2962 Size: 5392 PspXI vC'TCGA_Gb Cuts at: 2427 2427 Size: 5392 PstI C_TGCA'G Cuts at: 830 2744 830 Size: 1914 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5392 SalI G'TCGA_C Cuts at: 1936 2734 1936 Size: 798 4594 SbfI CC_TGCA'GG Cuts at: 2744 2744 Size: 5392 ScaI AGT'ACT Cuts at: 3640 5352 3640 Size: 1712 3680 SfcI C'TryA_G Cuts at: 826 1045 2740 3875 4556 4747 826 Size: 219 1695 1135 681 191 1471 Fragments arranged by size: 1695 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5392 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5392 SmaI CCC'GGG Cuts at: 1872 2719 1872 Size: 847 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2427 2483 3496 4397 4659 820 Size: 197 926 484 56 1013 901 262 1553 Fragments arranged by size: 1553 1013 926 901 484 262 197 56 SnaBI TAC'GTA Cuts at: 493 3183 493 Size: 2690 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5392 SphI G_CATG'C Cuts at: 1132 2045 2750 1132 Size: 913 705 3774 Fragments arranged by size: 3774 913 705 SspI AAT'ATT Cuts at: 5 52 2281 3316 5 Size: 47 2229 1035 2081 Fragments arranged by size: 2229 2081 1035 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5392 StyI C'CwwG_G Cuts at: 513 1065 2011 2148 2466 2831 513 Size: 552 946 137 318 365 3074 Fragments arranged by size: 3074 946 552 365 318 137 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2085 3419 3436 3589 3774 4219 325 Size: 1255 505 1334 17 153 185 445 1498 Fragments arranged by size: 1498 1334 1255 505 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2128 2368 3638 Size: 274 80 33 51 1443 151 240 1270 Cuts at: 3638 5350 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1270 274 240 151 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5352 5384 5352 Size: 32 5360 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5392 XbaI T'CTAG_A Cuts at: 2728 2728 Size: 5392 XhoI C'TCGA_G Cuts at: 1943 2427 1943 Size: 484 4908 XmaI C'CCGG_G Cuts at: 1870 2717 1870 Size: 847 4545 XmnI GAAnn'nnTTC Cuts at: 2055 3521 2055 Size: 1466 3926 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3200 276 Size: 53 83 186 936 1666 2468 Fragments arranged by size: 2468 1666 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspXI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI