(Circular) (Six-base) MAPSORT of: pFN21AB0996.seq Check: 6389 from: 1 to: 6100 pFN21AB0996 6100 bp With 182 enzymes: SgfI * April 15, 2010 09:33 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2057 2057 Size: 6100 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3910 278 Size: 53 83 186 936 2374 2468 Fragments arranged by size: 2468 2374 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2635 3443 1937 Size: 698 808 4594 Fragments arranged by size: 4594 808 698 Acc65I G'GTAC_C Cuts at: 3421 3421 Size: 6100 AclI AA'CG_TT Cuts at: 1563 4227 4600 1563 Size: 2664 373 3063 Fragments arranged by size: 3063 2664 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2465 2687 4163 5547 5815 1632 Size: 833 222 1476 1384 268 1917 Fragments arranged by size: 1917 1476 1384 833 268 222 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5903 AflIII A'CryG_T Cuts at: 1203 4999 5976 1203 Size: 3796 977 1327 Fragments arranged by size: 3796 1327 977 AgeI A'CCGG_T Cuts at: 4931 4931 Size: 6100 AhdI GACnn_n'nnGTC Cuts at: 4829 4829 Size: 6100 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6100 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6100 AlwNI CAG_nnn'CTG Cuts at: 2109 2479 2629 2798 3232 3843 5415 2109 Size: 370 150 169 434 611 1572 2794 Fragments arranged by size: 2794 1572 611 434 370 169 150 ApaLI G'TGCA_C Cuts at: 4157 5313 4157 Size: 1156 4944 ApoI r'AATT_y Cuts at: 1493 3243 3409 3635 3787 5896 1493 Size: 1750 166 226 152 2109 1697 Fragments arranged by size: 2109 1750 1697 226 166 152 AseI AT'TA_AT Cuts at: 160 1032 4654 160 Size: 872 3622 1606 Fragments arranged by size: 3622 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6100 AvaI C'yCGr_G Cuts at: 1870 1943 3425 1870 Size: 73 1482 4545 Fragments arranged by size: 4545 1482 73 BamHI G'GATC_C Cuts at: 2893 3430 3797 2893 Size: 537 367 5196 Fragments arranged by size: 5196 537 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2356 3421 4876 618 Size: 325 217 627 569 1065 1455 1842 Fragments arranged by size: 1842 1455 1065 627 569 325 217 BanII G_rGCy'C Cuts at: 1391 2197 2723 3419 1391 Size: 806 526 696 4072 Fragments arranged by size: 4072 806 696 526 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2400 2463 3046 928 Size: 971 501 63 583 3982 Fragments arranged by size: 3982 971 583 501 63 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4291 4325 1903 Size: 34 2354 34 3678 Fragments arranged by size: 3678 2354 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2948 3283 3992 5202 2948 Size: 335 709 1210 3846 Fragments arranged by size: 3846 1210 709 335 BclI T'GATC_A Cuts at: 1548 1548 Size: 6100 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4711 136 Size: 107 122 71 631 605 3039 1525 Fragments arranged by size: 3039 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6095 6095 Size: 6100 BlpI GC'TnA_GC Cuts at: 2722 3517 2722 Size: 795 5305 Bme1580I G_kGCm'C Cuts at: 1790 2359 2376 3119 4161 5317 1790 Size: 569 17 743 1042 1156 2573 Fragments arranged by size: 2573 1156 1042 743 569 17 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6100 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2075 2179 3831 Size: 416 53 83 399 24 658 104 1652 Cuts at: 3831 4789 442 Size: 958 1753 Fragments arranged by size: 1753 1652 958 658 416 399 104 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6100 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2818 2818 Size: 6100 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2408 2546 2620 2627 2723 4760 1239 Size: 1169 138 74 7 96 2037 2579 Fragments arranged by size: 2579 2037 1169 138 96 74 7 Bpu10I CC'TnA_GC Cuts at: 2063 2063 Size: 6100 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2666 4225 5090 5388 2666 Size: 1559 865 298 3378 Fragments arranged by size: 3378 1559 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4763 882 Size: 617 3264 2219 Fragments arranged by size: 3264 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3891 493 Size: 711 2687 2702 Fragments arranged by size: 2702 2687 711 BsaBI GATnn'nnATC Cuts at: 6094 6094 Size: 6100 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3907 4289 4924 275 Size: 53 83 186 936 2374 382 635 1451 Fragments arranged by size: 2374 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2541 3074 4533 4931 5205 5352 5763 2541 Size: 533 1459 398 274 147 411 2878 Fragments arranged by size: 2878 1459 533 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5875 Fragments arranged by size: 5875 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6100 BseYI C'CCAG_C Cuts at: 2233 5303 5799 2233 Size: 3070 496 2534 Fragments arranged by size: 3070 2534 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3128 1163 Size: 573 1392 4135 Fragments arranged by size: 4135 1392 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3550 4311 5339 1540 Size: 275 186 1549 761 1028 2301 Fragments arranged by size: 2301 1549 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4161 4246 5317 4161 Size: 85 1071 4944 Fragments arranged by size: 4944 1071 85 BsmI GAATG_Cn' Cuts at: 3611 3704 3611 Size: 93 6007 BsmBI CGTCTCn'nnnn_ Cuts at: 2255 2255 Size: 6100 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2197 2359 2376 2723 3119 3419 4161 Size: 399 407 162 17 347 396 300 742 Cuts at: 4161 4246 5317 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 407 399 396 347 300 162 85 17 BspEI T'CCGG_A Cuts at: 5763 5763 Size: 6100 BspHI T'CATG_A Cuts at: 3989 5719 3989 Size: 1730 4370 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2057 3455 844 Size: 1213 1398 3489 Fragments arranged by size: 3489 1398 1213 BsrBI CCG'CTC Cuts at: 1394 1823 2353 3987 1394 Size: 429 530 1634 3507 Fragments arranged by size: 3507 1634 530 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2496 2680 2833 4595 4769 65 Size: 1183 1248 184 153 1762 174 1396 Fragments arranged by size: 1762 1396 1248 1183 184 174 153 BsrFI r'CCGG_y Cuts at: 1700 1810 4744 4931 1700 Size: 110 2934 187 2869 Fragments arranged by size: 2934 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6100 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6100 BssSI C'ACGA_G Cuts at: 2949 4160 5172 2949 Size: 1211 1012 3877 Fragments arranged by size: 3877 1211 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3232 794 Size: 2438 3662 BstBI TT'CG_AA Cuts at: 4904 4904 Size: 6100 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6100 BstYI r'GATC_y Cuts at: 1972 2893 3430 3797 4182 4199 5651 5737 5749 Size: 921 537 367 385 17 1452 86 12 Cuts at: 5749 6095 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 921 537 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3034 3158 3034 Size: 124 5976 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4609 Fragments arranged by size: 4609 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5361 Fragments arranged by size: 5361 683 56 BtsI GCAGTG_nn' Cuts at: 3616 4410 4430 3616 Size: 794 20 5286 Fragments arranged by size: 5286 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6100 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2368 2403 529 Size: 35 1804 35 4226 Fragments arranged by size: 4226 1804 35 35 DraI TTT'AAA Cuts at: 2137 3404 2137 Size: 1267 4833 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5107 809 Size: 507 3791 1802 Fragments arranged by size: 3791 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3547 4436 6065 Size: 54 1425 50 275 18 1717 889 1629 Cuts at: 6065 8 Size: 43 Fragments arranged by size: 1717 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3547 1537 Size: 275 1735 4090 Fragments arranged by size: 4090 1735 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2577 2663 4030 1353 Size: 1224 86 1367 3423 Fragments arranged by size: 3423 1367 1224 86 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4687 5061 5207 387 Size: 1064 138 3098 374 146 1280 Fragments arranged by size: 3098 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2408 2465 2546 2620 2627 2687 2723 Size: 393 776 57 81 74 7 60 36 Cuts at: 2723 4163 4760 5547 5815 1239 Size: 1440 597 787 268 1524 Fragments arranged by size: 1524 1440 787 776 597 393 268 81 74 60 57 36 7 EcoNI CCTnn'n_nnAGG Cuts at: 2576 3390 2576 Size: 814 5286 EcoRI G'AATT_C Cuts at: 3409 3787 3409 Size: 378 5722 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2860 3343 2860 Size: 483 5617 FspI TGC'GCA Cuts at: 4606 4606 Size: 6100 HaeII r_GCGC'y Cuts at: 1678 2347 5247 1678 Size: 669 2900 2531 Fragments arranged by size: 2900 2531 669 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2523 2555 2878 2910 3125 Size: 32 135 32 951 32 323 32 215 Cuts at: 3125 3157 4741 4773 4815 4847 1373 Size: 32 1584 32 42 32 2626 Fragments arranged by size: 2626 1584 951 323 215 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3444 3690 669 Size: 988 281 1506 246 3079 Fragments arranged by size: 3079 1506 988 281 246 HindIII A'AGCT_T Cuts at: 748 2837 748 Size: 2089 4011 HpaI GTT'AAC Cuts at: 3690 3690 Size: 6100 KpnI G_GTAC'C Cuts at: 3425 3425 Size: 6100 MfeI C'AATT_G Cuts at: 3699 3699 Size: 6100 MluI A'CGCG_T Cuts at: 5976 5976 Size: 6100 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2242 5214 5398 1305 Size: 937 2972 184 2007 Fragments arranged by size: 2972 2007 937 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4278 Fragments arranged by size: 4278 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2489 3122 3219 4058 4417 4576 518 Size: 648 1323 633 97 839 359 159 2042 Fragments arranged by size: 2042 1323 839 648 633 359 159 97 MspA1I CmG'CkG Cuts at: 1606 2233 3516 4193 5341 5586 1606 Size: 627 1283 677 1148 245 2120 Fragments arranged by size: 2120 1283 1148 677 627 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4609 Fragments arranged by size: 4609 939 552 NdeI CA'TA_TG Cuts at: 387 2205 387 Size: 1818 4282 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6100 NotI GC'GGCC_GC Cuts at: 3547 3547 Size: 6100 NspI r_CATG'y Cuts at: 1132 1229 3458 5003 1132 Size: 97 2229 1545 2229 Fragments arranged by size: 2229 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6100 PciI A'CATG_T Cuts at: 4999 4999 Size: 6100 PflMI CCAn_nnn'nTGG Cuts at: 1108 2695 1108 Size: 1587 4513 PfoI T'CCnGG_A Cuts at: 2694 2984 2694 Size: 290 5810 PmeI GTTT'AAAC Cuts at: 3404 3404 Size: 6100 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2907 2939 4169 4201 5682 5714 2907 Size: 32 1230 32 1481 32 3293 Fragments arranged by size: 3293 1481 1230 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6100 PsiI TTA'TAA Cuts at: 3282 3670 3282 Size: 388 5712 PstI C_TGCA'G Cuts at: 830 2742 3452 830 Size: 1912 710 3478 Fragments arranged by size: 3478 1912 710 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6100 PvuII CAG'CTG Cuts at: 2233 2233 Size: 6100 SalI G'TCGA_C Cuts at: 1936 3442 1936 Size: 1506 4594 SapI GCTCTTCn'nnn_ Cuts at: 2663 2663 Size: 6100 SbfI CC_TGCA'GG Cuts at: 3452 3452 Size: 6100 ScaI AGT'ACT Cuts at: 4348 6060 4348 Size: 1712 4388 SfcI C'TryA_G Cuts at: 826 1045 2738 3448 4583 5264 5455 826 Size: 219 1693 710 1135 681 191 1471 Fragments arranged by size: 1693 1471 1135 710 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6100 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6100 SmaI CCC'GGG Cuts at: 1872 3427 1872 Size: 1555 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2681 4204 5105 5367 820 Size: 197 926 738 1523 901 262 1553 Fragments arranged by size: 1553 1523 926 901 738 262 197 SnaBI TAC'GTA Cuts at: 493 3891 493 Size: 3398 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6100 SphI G_CATG'C Cuts at: 1132 3458 1132 Size: 2326 3774 SspI AAT'ATT Cuts at: 5 52 3368 4024 5 Size: 47 3316 656 2081 Fragments arranged by size: 3316 2081 656 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6100 StyI C'CwwG_G Cuts at: 513 1065 2004 2805 3539 513 Size: 552 939 801 734 3074 Fragments arranged by size: 3074 939 801 734 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2975 4127 4144 4297 4482 4927 325 Size: 1255 1395 1152 17 153 185 445 1498 Fragments arranged by size: 1498 1395 1255 1152 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4346 6058 96 Size: 274 80 33 51 1443 2369 1712 138 Fragments arranged by size: 2369 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6060 6092 6060 Size: 32 6068 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6100 XbaI T'CTAG_A Cuts at: 3436 3436 Size: 6100 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2032 2230 2032 Size: 198 5902 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6100 XmaI C'CCGG_G Cuts at: 1870 3425 1870 Size: 1555 4545 XmnI GAAnn'nnTTC Cuts at: 2919 4229 2919 Size: 1310 4790 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3908 276 Size: 53 83 186 936 2374 2468 Fragments arranged by size: 2468 2374 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BstXI BstZ17I DraIII EcoICRI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI