Restriction map for pFN21AB0994
 (Linear) (Six-base) MAPSORT of: KIBB0994.seq  Check: 7774  from: 1  to: 1383



KIBB0994.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:15  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     726    1383 
   Size:        726     657
 
AccI GT'mk_AC

Cuts at:      0     845    1383 
   Size:        845     538
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     699    1383 
   Size:        699     684
 
AfeI AGC'GCT

Cuts at:      0    1348    1383 
   Size:       1348      35
 
AflIII A'CryG_T

Cuts at:      0      38     204    1383 
   Size:         38     166    1179

  Fragments arranged by size: 

                1179     166      38
 
AlwNI CAG_nnn'CTG

Cuts at:      0     247     789    1383 
   Size:        247     542     594

  Fragments arranged by size: 

                 594     542     247
 
ApaI G_GGCC'C

Cuts at:      0     514    1109    1160    1383 
   Size:        514     595      51     223

  Fragments arranged by size: 

                 595     514     223      51
 
ApaLI G'TGCA_C

Cuts at:      0     520    1383 
   Size:        520     863
 
BanI G'GyrC_C

Cuts at:      0     266    1383 
   Size:        266    1117
 
BanII G_rGCy'C

Cuts at:      0     368     514     614    1040    1109    1160    1340    1383 
   Size:        368     146     100     426      69      51     180      43

  Fragments arranged by size: 

                 426     368     180     146     100      69      51      43
            
 
BbvCI CC'TCA_GC

Cuts at:      0    1127    1210    1383 
   Size:       1127      83     173

  Fragments arranged by size: 

                1127     173      83
 
BclI T'GATC_A

Cuts at:      0     464    1383 
   Size:        464     919
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1123    1383 
   Size:       1123     260
 
Bme1580I G_kGCm'C

Cuts at:      0     271     487     514     524    1109    1160    1383 
   Size:        271     216      27      10     585      51     223

  Fragments arranged by size: 

                 585     271     223     216      51      27      10
 
BmgBI CAC'GTC

Cuts at:      0     205     480    1383 
   Size:        205     275     903

  Fragments arranged by size: 

                 903     275     205
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     497     517    1383 
   Size:        497      20     866

  Fragments arranged by size: 

                 866     497      20
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0       6     400     570     784    1000    1303    1318    1324 
   Size:          6     394     170     214     216     303      15       6

Cuts at:   1324    1381    1383 
   Size:         57       2

  Fragments arranged by size: 

                 394     303     216     214     170      57      15       6
                   6       2
 
Bpu10I CC'TnA_GC

Cuts at:      0    1127    1210    1383 
   Size:       1127      83     173

  Fragments arranged by size: 

                1127     173      83
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1159    1383 
   Size:       1159     224
 
BsaAI yAC'GTr

Cuts at:      0      39    1188    1383 
   Size:         39    1149     195

  Fragments arranged by size: 

                1149     195      39
 
BsaWI w'CCGG_w

Cuts at:      0     875    1383 
   Size:        875     508
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     760     790     904     934    1072    1102    1383 
   Size:        760      30     114      30     138      30     281

  Fragments arranged by size: 

                 760     281     138     114      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     781    1144    1153    1315    1318    1378    1383 
   Size:        781     363       9     162       3      60       5

  Fragments arranged by size: 

                 781     363     162      60       9       5       3
 
BseYI C'CCAG_C

Cuts at:      0     944    1137    1153    1273    1383 
   Size:        944     193      16     120     110

  Fragments arranged by size: 

                 944     193     120     110      16
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     294     467     695     895    1003    1351    1383 
   Size:        294     173     228     200     108     348      32

  Fragments arranged by size: 

                 348     294     228     200     173     108      32
 
BsiHKAI G_wGCw'C

Cuts at:      0     524    1340    1383 
   Size:        524     816      43

  Fragments arranged by size: 

                 816     524      43
 
BsmI GAATG_Cn'

Cuts at:      0     222    1383 
   Size:        222    1161
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      93    1383 
   Size:         93    1290
 
Bsp1286I G_dGCh'C

Cuts at:      0     271     368     487     514     524     614    1040    1109 
   Size:        271      97     119      27      10      90     426      69

Cuts at:   1109    1160    1340    1383 
   Size:         51     180      43

  Fragments arranged by size: 

                 426     271     180     119      97      90      69      51
                  43      27      10
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     439     726    1383 
   Size:        439     287     657

  Fragments arranged by size: 

                 657     439     287
 
BsrBI CCG'CTC

Cuts at:      0      20     732    1026    1383 
   Size:         20     712     294     357

  Fragments arranged by size: 

                 712     357     294      20
 
BsrDI GCAATG_nn'

Cuts at:      0     287    1040    1383 
   Size:        287     753     343

  Fragments arranged by size: 

                 753     343     287
 
BsrFI r'CCGG_y

Cuts at:      0       5      51    1383 
   Size:          5      46    1332

  Fragments arranged by size: 

                1332      46       5
 
BssSI C'ACGA_G

Cuts at:      0     652    1021    1383 
   Size:        652     369     362

  Fragments arranged by size: 

                 652     369     362
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      43     399    1383 
   Size:         43     356     984

  Fragments arranged by size: 

                 984     356      43
 
BstYI r'GATC_y

Cuts at:      0     329     698    1383 
   Size:        329     369     685

  Fragments arranged by size: 

                 685     369     329
 
BtgI C'CryG_G

Cuts at:      0     224     707    1325    1383 
   Size:        224     483     618      58

  Fragments arranged by size: 

                 618     483     224      58
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     243    1383 
   Size:        243    1140
 
BtsI GCAGTG_nn'

Cuts at:      0     683    1383 
   Size:        683     700
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     313    1383 
   Size:        313    1070
 
EaeI y'GGCC_r

Cuts at:      0      53     234     494    1383 
   Size:         53     181     260     889

  Fragments arranged by size: 

                 889     260     181      53
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      22    1383 
   Size:         22    1361
 
EcoICRI GAG'CTC

Cuts at:      0    1338    1383 
   Size:       1338      45
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0       6     400     570     699     784    1000    1303    1318 
   Size:          6     394     170     129      85     216     303      15

Cuts at:   1318    1324    1381    1383 
   Size:          6      57       2

  Fragments arranged by size: 

                 394     303     216     170     129      85      57      15
                   6       6       2
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1075    1383 
   Size:       1075     308
 
EcoO109I rG'GnC_Cy

Cuts at:      0     170     342     659     900    1083    1105    1157    1224 
   Size:        170     172     317     241     183      22      52      67

Cuts at:   1224    1383 
   Size:        159

  Fragments arranged by size: 

                 317     241     183     172     170     159      67      52
                  22
 
FspI TGC'GCA

Cuts at:      0     601    1383 
   Size:        601     782
 
FspAI rTGC'GCAy

Cuts at:      0     601    1383 
   Size:        601     782
 
HaeII r_GCGC'y

Cuts at:      0     745    1350    1383 
   Size:        745     605      33

  Fragments arranged by size: 

                 745     605      33
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     760     792    1041    1073    1273    1305    1383 
   Size:        760      32     249      32     200      32      78

  Fragments arranged by size: 

                 760     249     200      78      32      32      32
 
HincII GTy'rAC

Cuts at:      0     126    1218    1383 
   Size:        126    1092     165

  Fragments arranged by size: 

                1092     165     126
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     668    1046    1383 
   Size:        668     378     337

  Fragments arranged by size: 

                 668     378     337
 
MscI TGG'CCA

Cuts at:      0     236    1383 
   Size:        236    1147
 
MslI CAynn'nnrTG

Cuts at:      0     316    1045    1051    1383 
   Size:        316     729       6     332

  Fragments arranged by size: 

                 729     332     316       6
 
MspA1I CmG'CkG

Cuts at:      0     161     774     803     948    1383 
   Size:        161     613      29     145     435

  Fragments arranged by size: 

                 613     435     161     145      29
 
NaeI GCC'GGC

Cuts at:      0       7      53    1383 
   Size:          7      46    1330

  Fragments arranged by size: 

                1330      46       7
 
NgoMIV G'CCGG_C

Cuts at:      0       5      51    1383 
   Size:          5      46    1332

  Fragments arranged by size: 

                1332      46       5
 
NspI r_CATG'y

Cuts at:      0     966    1383 
   Size:        966     417
 
PasI CC'CwG_GG

Cuts at:      0     189    1383 
   Size:        189    1194
 
PfoI T'CCnGG_A

Cuts at:      0    1076    1383 
   Size:       1076     307
 
PmlI CAC'GTG

Cuts at:      0      39    1383 
   Size:         39    1344
 
PpuMI rG'GwC_Cy

Cuts at:      0     659    1083    1383 
   Size:        659     424     300

  Fragments arranged by size: 

                 659     424     300
 
PspOMI G'GGCC_C

Cuts at:      0     510    1105    1156    1383 
   Size:        510     595      51     227

  Fragments arranged by size: 

                 595     510     227      51
 
PstI C_TGCA'G

Cuts at:      0     785    1383 
   Size:        785     598
 
SacI G_AGCT'C

Cuts at:      0    1340    1383 
   Size:       1340      43
 
SanDI GG'GwC_CC

Cuts at:      0     659    1383 
   Size:        659     724
 
SbfI CC_TGCA'GG

Cuts at:      0     785    1383 
   Size:        785     598
 
SfcI C'TryA_G

Cuts at:      0     537     781    1383 
   Size:        537     244     602

  Fragments arranged by size: 

                 602     537     244
 
SmlI C'TyrA_G

Cuts at:      0    1174    1383 
   Size:       1174     209
 
SnaBI TAC'GTA

Cuts at:      0    1188    1383 
   Size:       1188     195
 
StuI AGG'CCT

Cuts at:      0     566    1383 
   Size:        566     817
 
StyI C'CwwG_G

Cuts at:      0      56     935    1383 
   Size:         56     879     448

  Fragments arranged by size: 

                 879     448      56
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     539    1110    1383 
   Size:        539     571     273

  Fragments arranged by size: 

                 571     539     273
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     904     936    1383 
   Size:        904      32     447

  Fragments arranged by size: 

                 904     447      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     283    1383 
   Size:        283    1100
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     504     975    1383 
   Size:        504     471     408

  Fragments arranged by size: 

                 504     471     408
 

 Enzymes that do cut:

     AarI     AccI     AcuI     AfeI   AflIII    AlwNI     ApaI    ApaLI
     BanI    BanII    BbvCI     BclI     BglI Bme1580I    BmgBI     BmrI
     BpmI   Bpu10I    BpuEI    BsaAI    BsaWI    BsaXI    BseRI    BseYI
     BsgI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspMI    BsrBI    BsrDI
    BsrFI    BssSI    BstXI    BstYI     BtgI    BtgZI     BtsI     DrdI
     EaeI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I     FspI    FspAI
    HaeII    Hin4I   HincII     MmeI     MscI     MslI   MspA1I     NaeI
   NgoMIV     NspI     PasI     PfoI     PmlI    PpuMI   PspOMI     PstI
     SacI    SanDI     SbfI     SfcI     SmlI    SnaBI     StuI     StyI
    TaqII     TstI  Tth111I     XcmI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI    AflII     AgeI     AhdI     AleI     AlfI
     AloI     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI
    BamHI     BbeI     BbsI     BcgI    BciVI    BfrBI    BglII     BlpI
     BmtI     BplI     BsaI    BsaBI    BsaHI    BsiEI    BsiWI    BspEI
    BspHI    BsrGI   BssHII   BstAPI    BstBI   BstEII  BstZ17I   Bsu36I
     ClaI    CspCI     DraI   DraIII     EagI     EarI    EcoRI    EcoRV
     FalI     FseI  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     NarI     NcoI     NdeI     NheI     NotI     NruI     NsiI     PacI
     PciI    PflMI     PmeI     PpiI    PshAI     PsiI    PspXI     PsrI
     PvuI    PvuII    RsrII    SacII     SalI     SapI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SpeI     SphI     SrfI
     SspI     SwaI     TatI     XbaI     XhoI     XmaI     XmnI     ZraI
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