(Circular) (Six-base) MAPSORT of: pFN21AB6882.seq Check: 277 from: 1 to: 5308 pFN21AB6882 5308 bp With 182 enzymes: SgfI * April 15, 2010 09:33 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3118 278 Size: 53 83 186 936 1582 2468 Fragments arranged by size: 2468 1582 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2379 2651 1937 Size: 442 272 4594 Fragments arranged by size: 4594 442 272 Acc65I G'GTAC_C Cuts at: 2629 2629 Size: 5308 AclI AA'CG_TT Cuts at: 1563 3435 3808 1563 Size: 1872 373 3063 Fragments arranged by size: 3063 1872 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3371 4755 5023 1632 Size: 1739 1384 268 1917 Fragments arranged by size: 1917 1739 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5111 AflIII A'CryG_T Cuts at: 1203 2262 2394 4207 5184 1203 Size: 1059 132 1813 977 1327 Fragments arranged by size: 1813 1327 1059 977 132 AgeI A'CCGG_T Cuts at: 4139 4139 Size: 5308 AhdI GACnn_n'nnGTC Cuts at: 4037 4037 Size: 5308 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5308 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5308 AlwNI CAG_nnn'CTG Cuts at: 3051 4623 3051 Size: 1572 3736 ApaLI G'TGCA_C Cuts at: 3365 4521 3365 Size: 1156 4152 ApoI r'AATT_y Cuts at: 1493 2617 2843 2995 5104 1493 Size: 1124 226 152 2109 1697 Fragments arranged by size: 2109 1697 1124 226 152 AseI AT'TA_AT Cuts at: 160 1032 3862 160 Size: 872 2830 1606 Fragments arranged by size: 2830 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5308 AvaI C'yCGr_G Cuts at: 1870 1943 2153 2472 2633 1870 Size: 73 210 319 161 4545 Fragments arranged by size: 4545 319 210 161 73 BamHI G'GATC_C Cuts at: 2638 3005 2638 Size: 367 4941 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2282 2438 2629 4084 618 Size: 325 217 627 495 156 191 1455 1842 Fragments arranged by size: 1842 1455 627 495 325 217 191 156 BanII G_rGCy'C Cuts at: 1391 2627 1391 Size: 1236 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4337 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2288 2322 2381 2415 3499 3533 1903 Size: 34 351 34 59 34 1084 34 3678 Fragments arranged by size: 3678 1084 351 59 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3200 4410 3200 Size: 1210 4098 BclI T'GATC_A Cuts at: 1548 1548 Size: 5308 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3919 136 Size: 107 122 71 631 605 2247 1525 Fragments arranged by size: 2247 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5303 5303 Size: 5308 BlpI GC'TnA_GC Cuts at: 2725 2725 Size: 5308 Bme1580I G_kGCm'C Cuts at: 1790 2285 3369 4525 1790 Size: 495 1084 1156 2573 Fragments arranged by size: 2573 1156 1084 495 BmgBI CAC'GTC Cuts at: 1318 2263 1318 Size: 945 4363 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2266 2338 3039 Size: 416 53 83 399 24 849 72 701 Cuts at: 3039 3997 442 Size: 958 1753 Fragments arranged by size: 1753 958 849 701 416 399 83 72 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5308 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2333 3968 1239 Size: 1094 1635 2579 Fragments arranged by size: 2579 1635 1094 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3433 4298 4596 3433 Size: 865 298 4145 Fragments arranged by size: 4145 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2198 3971 882 Size: 617 699 1773 2219 Fragments arranged by size: 2219 1773 699 617 BsaAI yAC'GTr Cuts at: 493 1204 2397 3099 493 Size: 711 1193 702 2702 Fragments arranged by size: 2702 1193 711 702 BsaBI GATnn'nnATC Cuts at: 5302 5302 Size: 5308 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3115 3497 4132 275 Size: 53 83 186 936 1582 382 635 1451 Fragments arranged by size: 1582 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3741 4139 4413 4560 4971 3741 Size: 398 274 147 411 4078 Fragments arranged by size: 4078 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2441 2471 2519 2549 1178 Size: 30 165 30 1038 30 48 30 3937 Fragments arranged by size: 3937 1038 165 48 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2543 1186 Size: 1357 3951 BseYI C'CCAG_C Cuts at: 2041 4511 5007 2041 Size: 2470 496 2342 Fragments arranged by size: 2470 2342 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2426 1163 Size: 573 690 4045 Fragments arranged by size: 4045 690 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2053 2758 3519 4547 1540 Size: 275 186 52 705 761 1028 2301 Fragments arranged by size: 2301 1028 761 705 275 186 52 BsiHKAI G_wGCw'C Cuts at: 3369 3454 4525 3369 Size: 85 1071 4152 Fragments arranged by size: 4152 1071 85 BsmI GAATG_Cn' Cuts at: 2819 2912 2819 Size: 93 5215 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2285 2627 3369 3454 4525 1391 Size: 399 495 342 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 495 399 342 85 BspEI T'CCGG_A Cuts at: 4971 4971 Size: 5308 BspHI T'CATG_A Cuts at: 3197 4927 3197 Size: 1730 3578 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2663 844 Size: 1819 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3195 1394 Size: 429 1372 3507 Fragments arranged by size: 3507 1372 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3803 3977 65 Size: 1183 2555 174 1396 Fragments arranged by size: 2555 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2207 3952 4139 1700 Size: 110 397 1745 187 2869 Fragments arranged by size: 2869 1745 397 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5308 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5308 BssSI C'ACGA_G Cuts at: 3368 4380 3368 Size: 1012 4296 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5308 BstBI TT'CG_AA Cuts at: 4112 4112 Size: 5308 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5308 BstYI r'GATC_y Cuts at: 1972 2638 3005 3390 3407 4859 4945 4957 5303 Size: 666 367 385 17 1452 86 12 346 Cuts at: 5303 1972 Size: 1977 Fragments arranged by size: 1977 1452 666 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2136 2196 513 Size: 552 1071 60 3625 Fragments arranged by size: 3625 1071 552 60 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2595 487 Size: 683 56 1369 3200 Fragments arranged by size: 3200 1369 683 56 BtsI GCAGTG_nn' Cuts at: 2824 3618 3638 2824 Size: 794 20 4494 Fragments arranged by size: 4494 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5308 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5273 DraI TTT'AAA Cuts at: 2612 2612 Size: 5308 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2180 4315 809 Size: 507 864 2135 1802 Fragments arranged by size: 2135 1802 864 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2050 2139 2209 Size: 54 1425 50 275 18 220 89 70 Cuts at: 2209 2541 2755 3644 5273 8 Size: 332 214 889 1629 43 Fragments arranged by size: 1629 1425 889 332 275 220 214 89 70 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2050 2755 1537 Size: 275 238 705 4090 Fragments arranged by size: 4090 705 275 238 EarI CTCTTCn'nnn_ Cuts at: 1353 3238 1353 Size: 1885 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3895 4269 4415 387 Size: 1064 138 2306 374 146 1280 Fragments arranged by size: 2306 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2333 3371 3968 4755 5023 1239 Size: 393 701 1038 597 787 268 1524 Fragments arranged by size: 1524 1038 787 701 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2124 2149 2158 2302 2476 2124 Size: 25 9 144 174 4956 Fragments arranged by size: 4956 174 144 25 9 EcoRI G'AATT_C Cuts at: 2617 2995 2617 Size: 378 4930 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2116 2131 2116 Size: 15 5293 FspI TGC'GCA Cuts at: 2269 3814 2269 Size: 1545 3763 HaeII r_GCGC'y Cuts at: 1678 2024 2042 2223 4455 1678 Size: 346 18 181 2232 2531 Fragments arranged by size: 2531 2232 346 181 18 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2441 2473 2573 2605 3949 Size: 32 135 32 869 32 100 32 1344 Cuts at: 3949 3981 4023 4055 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1344 869 135 100 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2380 2652 2898 669 Size: 988 281 442 272 246 3079 Fragments arranged by size: 3079 988 442 281 272 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5308 HpaI GTT'AAC Cuts at: 2898 2898 Size: 5308 KpnI G_GTAC'C Cuts at: 2633 2633 Size: 5308 MfeI C'AATT_G Cuts at: 2907 2907 Size: 5308 MluI A'CGCG_T Cuts at: 5184 5184 Size: 5308 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4422 4606 1305 Size: 3117 184 2007 Fragments arranged by size: 3117 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2141 2543 10 Size: 54 1425 343 309 402 2775 Fragments arranged by size: 2775 1425 402 343 309 54 MslI CAynn'nnrTG Cuts at: 518 1166 3266 3625 3784 518 Size: 648 2100 359 159 2042 Fragments arranged by size: 2100 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2049 2521 2724 3401 4549 4794 1606 Size: 443 472 203 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 472 443 245 203 NcoI C'CATG_G Cuts at: 513 1065 2136 513 Size: 552 1071 3685 Fragments arranged by size: 3685 1071 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5308 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5308 NotI GC'GGCC_GC Cuts at: 2050 2755 2050 Size: 705 4603 NspI r_CATG'y Cuts at: 1132 1229 2666 4211 1132 Size: 97 1437 1545 2229 Fragments arranged by size: 2229 1545 1437 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5308 PciI A'CATG_T Cuts at: 4207 4207 Size: 5308 PflMI CCAn_nnn'nTGG Cuts at: 1108 2010 1108 Size: 902 4406 PmeI GTTT'AAAC Cuts at: 2612 2612 Size: 5308 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3377 3409 4890 4922 3377 Size: 32 1481 32 3763 Fragments arranged by size: 3763 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2124 2149 2302 2476 2124 Size: 25 153 174 4956 Fragments arranged by size: 4956 174 153 25 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5308 PsiI TTA'TAA Cuts at: 2878 2878 Size: 5308 PstI C_TGCA'G Cuts at: 830 2172 2660 830 Size: 1342 488 3478 Fragments arranged by size: 3478 1342 488 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5308 SalI G'TCGA_C Cuts at: 1936 2378 2650 1936 Size: 442 272 4594 Fragments arranged by size: 4594 442 272 SanDI GG'GwC_CC Cuts at: 2476 2476 Size: 5308 SbfI CC_TGCA'GG Cuts at: 2660 2660 Size: 5308 ScaI AGT'ACT Cuts at: 2327 2411 3556 5268 2327 Size: 84 1145 1712 2367 Fragments arranged by size: 2367 1712 1145 84 SexAI A'CCwGG_T Cuts at: 2126 2126 Size: 5308 SfcI C'TryA_G Cuts at: 826 1045 2168 2656 3791 4472 4663 826 Size: 219 1123 488 1135 681 191 1471 Fragments arranged by size: 1471 1135 1123 681 488 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5308 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5308 SmaI CCC'GGG Cuts at: 1872 2474 2635 1872 Size: 602 161 4545 Fragments arranged by size: 4545 602 161 SmlI C'TyrA_G Cuts at: 820 1017 1943 3412 4313 4575 820 Size: 197 926 1469 901 262 1553 Fragments arranged by size: 1553 1469 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3099 493 Size: 2606 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5308 SphI G_CATG'C Cuts at: 1132 2666 1132 Size: 1534 3774 SspI AAT'ATT Cuts at: 5 52 3232 5 Size: 47 3180 2081 Fragments arranged by size: 3180 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5308 StyI C'CwwG_G Cuts at: 513 1065 2136 2229 2747 513 Size: 552 1071 93 518 3074 Fragments arranged by size: 3074 1071 552 518 93 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2002 3335 3352 3505 3690 4135 325 Size: 1255 422 1333 17 153 185 445 1498 Fragments arranged by size: 1498 1333 1255 445 422 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2325 2409 2550 Size: 274 80 33 51 1443 348 84 141 Cuts at: 2550 3554 5266 96 Size: 1004 1712 138 Fragments arranged by size: 1712 1443 1004 348 274 141 138 84 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2519 2551 5268 5300 2519 Size: 32 2717 32 2527 Fragments arranged by size: 2717 2527 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5308 XbaI T'CTAG_A Cuts at: 2644 2644 Size: 5308 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5308 XmaI C'CCGG_G Cuts at: 1870 2472 2633 1870 Size: 602 161 4545 Fragments arranged by size: 4545 602 161 XmnI GAAnn'nnTTC Cuts at: 3437 3437 Size: 5308 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3116 276 Size: 53 83 186 936 1582 2468 Fragments arranged by size: 2468 1582 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SapI SfiI SfoI SrfI SwaI XcmI