(Circular) (Six-base) MAPSORT of: pFN21AB0982.seq Check: 6618 from: 1 to: 6046 pFN21AB0982 6046 bp With 182 enzymes: SgfI * April 15, 2010 09:33 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3856 278 Size: 53 83 186 936 2320 2468 Fragments arranged by size: 2468 2320 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2577 3070 3389 1937 Size: 640 493 319 4594 Fragments arranged by size: 4594 640 493 319 Acc65I G'GTAC_C Cuts at: 3367 3367 Size: 6046 AclI AA'CG_TT Cuts at: 1563 4173 4546 1563 Size: 2610 373 3063 Fragments arranged by size: 3063 2610 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2811 2916 3145 4109 5493 5761 1632 Size: 1179 105 229 964 1384 268 1917 Fragments arranged by size: 1917 1384 1179 964 268 229 105 AfeI AGC'GCT Cuts at: 3098 3098 Size: 6046 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5849 AflIII A'CryG_T Cuts at: 1203 4945 5922 1203 Size: 3742 977 1327 Fragments arranged by size: 3742 1327 977 AgeI A'CCGG_T Cuts at: 4877 4877 Size: 6046 AhdI GACnn_n'nnGTC Cuts at: 4775 4775 Size: 6046 AleI CACnn'nnGTG Cuts at: 1166 3107 1166 Size: 1941 4105 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6046 AlwNI CAG_nnn'CTG Cuts at: 2149 3789 5361 2149 Size: 1640 1572 2834 Fragments arranged by size: 2834 1640 1572 ApaI G_GGCC'C Cuts at: 2471 2471 Size: 6046 ApaLI G'TGCA_C Cuts at: 2306 4103 5259 2306 Size: 1797 1156 3093 Fragments arranged by size: 3093 1797 1156 ApoI r'AATT_y Cuts at: 1493 3355 3581 3733 5842 1493 Size: 1862 226 152 2109 1697 Fragments arranged by size: 2109 1862 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4600 160 Size: 872 3568 1606 Fragments arranged by size: 3568 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6046 AvaI C'yCGr_G Cuts at: 1870 1943 2805 2994 3087 3371 1870 Size: 73 862 189 93 284 4545 Fragments arranged by size: 4545 862 284 189 93 73 BamHI G'GATC_C Cuts at: 3312 3376 3743 3312 Size: 64 367 5615 Fragments arranged by size: 5615 367 64 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2484 3202 3367 4822 618 Size: 325 217 627 697 718 165 1455 1842 Fragments arranged by size: 1842 1455 718 697 627 325 217 165 BanII G_rGCy'C Cuts at: 1391 2471 2993 3365 1391 Size: 1080 522 372 4072 Fragments arranged by size: 4072 1080 522 372 BbeI G_GCGC'C Cuts at: 2488 2488 Size: 6046 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5075 BbvCI CC'TCA_GC Cuts at: 2695 2956 2695 Size: 261 5785 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4237 4271 1903 Size: 34 2300 34 3678 Fragments arranged by size: 3678 2300 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3938 5148 3938 Size: 1210 4836 BclI T'GATC_A Cuts at: 1548 1548 Size: 6046 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2042 2051 2482 Size: 107 122 71 631 605 370 9 431 Cuts at: 2482 2723 4657 136 Size: 241 1934 1525 Fragments arranged by size: 1934 1525 631 605 431 370 241 122 107 71 9 BglII A'GATC_T Cuts at: 6041 6041 Size: 6046 BlpI GC'TnA_GC Cuts at: 3463 3463 Size: 6046 Bme1580I G_kGCm'C Cuts at: 1790 2310 2471 3207 4107 5263 1790 Size: 520 161 736 900 1156 2573 Fragments arranged by size: 2573 1156 900 736 520 161 BmgBI CAC'GTC Cuts at: 1318 2281 1318 Size: 963 5083 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2347 2485 2772 Size: 416 53 83 399 24 930 138 287 Cuts at: 2772 2808 2814 3777 4735 442 Size: 36 6 963 958 1753 Fragments arranged by size: 1753 963 958 930 416 399 287 138 83 53 36 24 6 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6046 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2383 2383 Size: 6046 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2378 2515 2865 4706 1239 Size: 1139 137 350 1841 2579 Fragments arranged by size: 2579 1841 1139 350 137 Bpu10I CC'TnA_GC Cuts at: 2695 2956 2695 Size: 261 5785 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4171 5036 5334 4171 Size: 865 298 4883 Fragments arranged by size: 4883 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2777 4709 882 Size: 617 1278 1932 2219 Fragments arranged by size: 2219 1932 1278 617 BsaAI yAC'GTr Cuts at: 493 1204 2253 3014 3837 493 Size: 711 1049 761 823 2702 Fragments arranged by size: 2702 1049 823 761 711 BsaBI GATnn'nnATC Cuts at: 6040 6040 Size: 6046 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2485 3067 3853 4235 Size: 53 83 186 936 952 582 786 382 Cuts at: 4235 4870 275 Size: 635 1451 Fragments arranged by size: 1451 952 936 786 635 582 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3306 4479 4877 5151 5298 5709 3306 Size: 1173 398 274 147 411 3643 Fragments arranged by size: 3643 1173 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2009 2039 2501 2531 2906 Size: 30 165 30 606 30 462 30 375 Cuts at: 2906 2936 1178 Size: 30 4288 Fragments arranged by size: 4288 606 462 375 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2789 2915 2918 2921 2924 2927 1186 Size: 1603 126 3 3 3 3 4305 Fragments arranged by size: 4305 1603 126 3 3 3 3 BseYI C'CCAG_C Cuts at: 2227 2472 5249 5745 2227 Size: 245 2777 496 2528 Fragments arranged by size: 2777 2528 496 245 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2051 2275 1163 Size: 573 315 224 4934 Fragments arranged by size: 4934 573 315 224 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3496 4257 5285 1540 Size: 275 186 1495 761 1028 2301 Fragments arranged by size: 2301 1495 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2286 2310 3027 3141 4107 4192 5263 2286 Size: 24 717 114 966 85 1071 3069 Fragments arranged by size: 3069 1071 966 717 114 85 24 BsmI GAATG_Cn' Cuts at: 3557 3650 3557 Size: 93 5953 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2286 2310 2471 2993 3027 3141 3207 Size: 399 496 24 161 522 34 114 66 Cuts at: 3207 3365 4107 4192 5263 1391 Size: 158 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 522 496 399 161 158 114 85 66 34 24 BspEI T'CCGG_A Cuts at: 3306 5709 3306 Size: 2403 3643 BspHI T'CATG_A Cuts at: 2565 3935 5665 2565 Size: 1370 1730 2946 Fragments arranged by size: 2946 1730 1370 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2589 3401 844 Size: 1745 812 3489 Fragments arranged by size: 3489 1745 812 BsrBI CCG'CTC Cuts at: 1394 1823 2127 3933 1394 Size: 429 304 1806 3507 Fragments arranged by size: 3507 1806 429 304 BsrDI GCAATG_nn' Cuts at: 65 1248 4541 4715 65 Size: 1183 3293 174 1396 Fragments arranged by size: 3293 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4690 4877 1700 Size: 110 2880 187 2869 Fragments arranged by size: 2880 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6046 BssHII G'CGCG_C Cuts at: 1925 2134 2352 1925 Size: 209 218 5619 Fragments arranged by size: 5619 218 209 BssSI C'ACGA_G Cuts at: 4106 5118 4106 Size: 1012 5034 BstAPI GCAn_nnn'nTGC Cuts at: 794 2256 2394 794 Size: 1462 138 4446 Fragments arranged by size: 4446 1462 138 BstBI TT'CG_AA Cuts at: 4850 4850 Size: 6046 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6046 BstXI CCAn_nnnn'nTGG Cuts at: 2326 2823 2326 Size: 497 5549 BstYI r'GATC_y Cuts at: 1972 2540 3312 3376 3743 4128 4145 5597 5683 Size: 568 772 64 367 385 17 1452 86 Cuts at: 5683 5695 6041 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 772 568 385 367 346 86 64 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2677 3208 3326 513 Size: 552 939 673 531 118 3233 Fragments arranged by size: 3233 939 673 552 531 118 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2404 487 Size: 683 56 1178 4129 Fragments arranged by size: 4129 1178 683 56 BtsI GCAGTG_nn' Cuts at: 2943 3065 3193 3562 4356 4376 2943 Size: 122 128 369 794 20 4613 Fragments arranged by size: 4613 794 369 128 122 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6046 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6011 DraI TTT'AAA Cuts at: 3350 3350 Size: 6046 DraIII CAC_nnn'GTG Cuts at: 2256 2412 2256 Size: 156 5890 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5053 809 Size: 507 3737 1802 Fragments arranged by size: 3737 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2043 2055 2166 Size: 54 1425 50 275 18 213 12 111 Cuts at: 2166 2187 2214 3003 3016 3493 4382 6011 8 Size: 21 27 789 13 477 889 1629 43 Fragments arranged by size: 1629 1425 889 789 477 275 213 111 54 50 43 27 21 18 13 12 EagI C'GGCC_G Cuts at: 1537 1812 3493 1537 Size: 275 1681 4090 Fragments arranged by size: 4090 1681 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2291 2892 2913 3221 3976 1353 Size: 938 601 21 308 755 3423 Fragments arranged by size: 3423 938 755 601 308 21 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3284 4633 5007 5153 387 Size: 1064 138 1695 1349 374 146 1280 Fragments arranged by size: 1695 1349 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2378 2515 2811 2865 2916 3145 4109 Size: 393 746 137 296 54 51 229 964 Cuts at: 4109 4706 5493 5761 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 964 787 746 597 393 296 268 229 137 54 51 EcoNI CCTnn'n_nnAGG Cuts at: 3307 3307 Size: 6046 EcoO109I rG'GnC_Cy Cuts at: 2017 2467 2468 2621 2639 2802 2017 Size: 450 1 153 18 163 5261 Fragments arranged by size: 5261 450 163 153 18 1 EcoRI G'AATT_C Cuts at: 3355 3733 3355 Size: 378 5668 FspI TGC'GCA Cuts at: 2585 4552 2585 Size: 1967 4079 HaeII r_GCGC'y Cuts at: 1678 2276 2488 3100 5193 1678 Size: 598 212 612 2093 2531 Fragments arranged by size: 2531 2093 612 598 212 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2009 2041 4687 4719 4761 Size: 32 135 32 437 32 2646 32 42 Cuts at: 4761 4793 1373 Size: 32 2626 Fragments arranged by size: 2646 2626 437 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2118 2743 3071 3390 3636 669 Size: 988 281 180 625 328 319 246 3079 Fragments arranged by size: 3079 988 625 328 319 281 246 180 HindIII A'AGCT_T Cuts at: 748 748 Size: 6046 HpaI GTT'AAC Cuts at: 3636 3636 Size: 6046 KasI G'GCGC_C Cuts at: 2484 2484 Size: 6046 KpnI G_GTAC'C Cuts at: 3371 3371 Size: 6046 MfeI C'AATT_G Cuts at: 3645 3645 Size: 6046 MluI A'CGCG_T Cuts at: 5922 5922 Size: 6046 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5160 5344 1305 Size: 3855 184 2007 Fragments arranged by size: 3855 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2189 2216 3005 3018 10 Size: 54 1425 343 357 27 789 13 3038 Fragments arranged by size: 3038 1425 789 357 343 54 27 13 MslI CAynn'nnrTG Cuts at: 518 1166 2183 3107 4004 4363 4522 518 Size: 648 1017 924 897 359 159 2042 Fragments arranged by size: 2042 1017 924 897 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2042 2974 3082 3462 4139 5287 5532 1606 Size: 436 932 108 380 677 1148 245 2120 Fragments arranged by size: 2120 1148 932 677 436 380 245 108 NarI GG'CG_CC Cuts at: 2485 2485 Size: 6046 NcoI C'CATG_G Cuts at: 513 1065 2004 2677 3208 513 Size: 552 939 673 531 3351 Fragments arranged by size: 3351 939 673 552 531 NdeI CA'TA_TG Cuts at: 387 387 Size: 6046 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6046 NotI GC'GGCC_GC Cuts at: 3493 3493 Size: 6046 NspI r_CATG'y Cuts at: 1132 1229 3404 4949 1132 Size: 97 2175 1545 2229 Fragments arranged by size: 2229 2175 1545 97 PasI CC'CwG_GG Cuts at: 1348 2625 1348 Size: 1277 4769 PciI A'CATG_T Cuts at: 4945 4945 Size: 6046 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6046 PmeI GTTT'AAAC Cuts at: 3350 3350 Size: 6046 PmlI CAC'GTG Cuts at: 2253 2253 Size: 6046 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4115 4147 5628 5660 4115 Size: 32 1481 32 4501 Fragments arranged by size: 4501 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2017 2621 2802 2017 Size: 604 181 5261 Fragments arranged by size: 5261 604 181 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6046 PsiI TTA'TAA Cuts at: 3616 3616 Size: 6046 PspOMI G'GGCC_C Cuts at: 2467 2467 Size: 6046 PstI C_TGCA'G Cuts at: 830 2979 3398 830 Size: 2149 419 3478 Fragments arranged by size: 3478 2149 419 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6046 PvuII CAG'CTG Cuts at: 3082 3082 Size: 6046 SalI G'TCGA_C Cuts at: 1936 3069 3388 1936 Size: 1133 319 4594 Fragments arranged by size: 4594 1133 319 SanDI GG'GwC_CC Cuts at: 2802 2802 Size: 6046 SapI GCTCTTCn'nnn_ Cuts at: 2291 2291 Size: 6046 SbfI CC_TGCA'GG Cuts at: 3398 3398 Size: 6046 ScaI AGT'ACT Cuts at: 4294 6006 4294 Size: 1712 4334 SfcI C'TryA_G Cuts at: 826 1045 2975 3048 3166 3394 4529 5210 5401 Size: 219 1930 73 118 228 1135 681 191 Cuts at: 5401 826 Size: 1471 Fragments arranged by size: 1930 1471 1135 681 228 219 191 118 73 SfiI GGCCn_nnn'nGGCC Cuts at: 2042 2042 Size: 6046 SfoI GGC'GCC Cuts at: 2486 2486 Size: 6046 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6046 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6046 SmaI CCC'GGG Cuts at: 1872 2807 2996 3089 3373 1872 Size: 935 189 93 284 4545 Fragments arranged by size: 4545 935 284 189 93 SmlI C'TyrA_G Cuts at: 820 1017 1943 4150 5051 5313 820 Size: 197 926 2207 901 262 1553 Fragments arranged by size: 2207 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3837 493 Size: 3344 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6046 SphI G_CATG'C Cuts at: 1132 3404 1132 Size: 2272 3774 SspI AAT'ATT Cuts at: 5 52 3970 5 Size: 47 3918 2081 Fragments arranged by size: 3918 2081 47 StuI AGG'CCT Cuts at: 1517 2513 1517 Size: 996 5050 StyI C'CwwG_G Cuts at: 513 1065 2004 2631 2677 3208 3485 513 Size: 552 939 627 46 531 277 3074 Fragments arranged by size: 3074 939 627 552 531 277 46 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2833 3032 4073 4090 4243 4428 4873 Size: 1255 1253 199 1041 17 153 185 445 Cuts at: 4873 325 Size: 1498 Fragments arranged by size: 1498 1255 1253 1041 445 199 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2361 4292 6004 Size: 274 80 33 51 1443 384 1931 1712 Cuts at: 6004 96 Size: 138 Fragments arranged by size: 1931 1712 1443 384 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2741 2773 2906 2938 6006 6038 2741 Size: 32 133 32 3068 32 2749 Fragments arranged by size: 3068 2749 133 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6046 XbaI T'CTAG_A Cuts at: 3382 3382 Size: 6046 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2224 2224 Size: 6046 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6046 XmaI C'CCGG_G Cuts at: 1870 2805 2994 3087 3371 1870 Size: 935 189 93 284 4545 Fragments arranged by size: 4545 935 284 189 93 XmnI GAAnn'nnTTC Cuts at: 2150 3098 4175 2150 Size: 948 1077 4021 Fragments arranged by size: 4021 1077 948 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3854 276 Size: 53 83 186 936 2320 2468 Fragments arranged by size: 2468 2320 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PspXI PsrI RsrII SacI SacII SexAI SrfI SwaI