Restriction map for
pFN21AB0979
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# NO CUT ENZYMES ( 183 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspI AvaI AvaII AviII
AvrII BamHI BanI BbeI BbrPI
BbsI BbvCI BcgI BciVI BclI
BcnI BfrI BglII BlnI BpuAI
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BsgI BsiEI BsiWI BsiYI
BslI BsmAI BsmBI BsmI BsoBI
Bsp1407I BspDI BspEI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstYI BstZ17I Bsu36I
CfoI Cfr10I Cfr13I ClaI CpoI
DraI DraII DraIII DrdI DsaI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoT14I EcoT22I FbaI FseI
FspI HaeII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HpaI HpaII KasI KpnI Ksp632I
KspI MamI MfeI MflI MluI
MroI MslI MspI MunI MvnI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI RsrII SacII SalI SapI
Sau96I SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI Tsp45I
Tth111I Van91I XbaI XcmI XhoI
XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 32 enzymes) #
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AciI 483 GCGG CCGC(-3/-1)
AflIII 66 ACATGT A^C[GA][CT]GT
Asp700I 106 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 162 TGGCCA TGG^CCA
BanII 360 GAGCTC G[GA]GC[CT]^C
BfaI 416 CTAG C^TAG
BglI 678 GCCCCACAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 127 GCTGAGC GC^T[ACGT]AGC
BpmI 587 CTGGAG CTGGAG(16/14)
Bpu1102I 127 GCTGAGC GC^T[ACGT]AGC
BsaJI 196 CCTGGG C^C[ACGT][ACGT]GG
BsmFI 3 GGGAC GGGAC(10/14)
BspHI 290 TCATGA T^CATGA
BspLU11I 66 ACATGT A^CATGT
CelII 127 GCTGAGC GC^T[ACGT]AGC
DdeI 128 CTGAG C^T[ACGT]AG
EaeI 162 TGGCCA [CT]^GGCC[GA]
EcoRV 325 GATATC GAT^ATC
FauI 483 GCGGG CCCGC(4/6)
HphI 435 GGTGA GGTGA(8/7)
MaeI 416 CTAG C^TAG
MaeII 73 ACGT A^CGT
MluNI 162 TGGCCA TGG^CCA
MscI 162 TGGCCA TGG^CCA
MspA1I 442 CAGCTG C[AC]G^C[GT]G
NlaIV 91 GGCTCC GG[ACGT]^[ACGT]CC
NspI 66 ACATGT [GA]CATG^[CT]
PvuII 442 CAGCTG CAG^CTG
RcaI 290 TCATGA T^CATGA
SacI 360 GAGCTC GAGCT^C
SfcI 577 CTACAG C^T[GA][CT]AG
XmnI 106 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AlwI 18 GGATC GGATC(4/5)
287 GGATC
AspHI 360 GAGCTC G[AT]GC[AT]^C
506 GTGCTC
BbvI 340 GCAGC GCAGC(8/12)
441 GCAGC
BmyI 360 GAGCTC G[AGT]GC[ACT]^C
506 GTGCTC
BseRI 454 GAGGAG GAGGAG(10/8)
457 GAGGAG
BsiHKAI 360 GAGCTC G[AT]GC[AT]^C
506 GTGCTC
Bsp1286I 360 GAGCTC G[AGT]GC[ACT]^C
506 GTGCTC
BsrI 586 ACTGG ACTGG(1/-1)
303 CCAGT
BssKI 165 CCAGG ^CC[ACGT]GG
196 CCTGG
BstNI 165 CCAGG CC^[AT]GG
196 CCTGG
EcoRII 165 CCAGG ^CC[AT]GG
196 CCTGG
Fnu4HI 340 GCAGC GC^[ACGT]GC
441 GCAGC
HinfI 205 GATTC G^A[ACGT]TC
598 GAATC
ItaI 340 GCAGC GC^[ACGT]GC
441 GCAGC
MvaI 165 CCAGG CC^[AT]GG
196 CCTGG
ScrFI 165 CCAGG CC^[ACGT]GG
196 CCTGG
SfaNI 616 GATGC GCATC(5/9)
280 GCATC
TaqI 81 TCGA T^CGA
549 TCGA
TfiI 205 GATTC G^A[AT]TC
598 GAATC
TseI 340 GCAGC G^C[AT]GC
441 GCAGC
TspRI 264 CAGTG CA[GC]TG[ACGT][ACGT]^
624 CACTG
TthHB8I 81 TCGA T^CGA
549 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 21 enzymes) #
#########################################
AcsI AfaI AluI ApoI BstF5I
Cac8I DpnII FokI HaeIII MaeIII
MboI MboII MnlI MseI MwoI
NdeII NlaIII RsaI Sau3AI Tru9I
Tsp509I