Restriction map for
pFN21AB0977
##################################
# NO CUT ENZYMES ( 184 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AciI AclI AcsI AcyI AflII
AflIII AgeI AhdI Alw44I AlwI
AlwNI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp718I AspEI
AspHI AspI AviII BalI BamHI
BanI BanII BbeI BbrPI BbsI
BbvCI BciVI BclI BcnI BfrI
BglI BglII BlpI BmyI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsaWI
BseAI BseRI BsgI BsiEI BsiHKAI
BsiWI BsmFI BsmI Bsp1286I Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstYI BstZ17I
CelII Cfr10I ClaI CpoI DraI
DraII DrdI EaeI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoO109I
EcoO65I EcoRI EcoRV EcoT22I FauI
FbaI FseI FspI HaeII HapII
HgaI Hin1I HindIII HpaII KasI
KpnI KspI MaeII MaeIII MamI
MfeI MflI MluI MluNI MroI
MscI MspI MunI MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfcI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI Tsp45I Tsp509I
TspRI Tth111I Van91I XbaI XcmI
XhoI XhoII XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AfaI 22 GTAC GT^AC
AluI 413 AGCT AG^CT
Asp700I 359 GAAATGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 337 CCCGAG C^[CT]CG[GA]G
AvrII 31 CCTAGG C^CTAGG
BbvI 414 GCTGC GCAGC(8/12)
BcgI 75 CGATGATATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI 31 CCTAGG C^CTAGG
BpmI 310 CTCCAG CTGGAG(16/14)
BsaI 27 GAGACC GGTCTC(1/5)
BsmBI 8 GAGACG CGTCTC(1/5)
BsoBI 337 CCCGAG C^[CT]CG[GA]G
BsrI 519 ACTGG ACTGG(1/-1)
BstF5I 286 CATCC GGATG(2/0)
CfoI 417 GCGC GCG^C
DraIII 496 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 267 CCGTGG C^C[GA][CT]GG
EcoT14I 31 CCTAGG C^C[AT][AT]GG
Fnu4HI 414 GCTGC GC^[ACGT]GC
FokI 286 CATCC GGATG(9/13)
HhaI 417 GCGC GCG^C
HinP1I 417 GCGC G^CGC
HincII 451 GTTAAC GT[CT]^[GA]AC
HindII 451 GTTAAC GT[CT]^[GA]AC
HpaI 451 GTTAAC GTT^AAC
HphI 522 GGTGA GGTGA(8/7)
ItaI 414 GCTGC GC^[ACGT]GC
MseI 452 TTAA T^TAA
MslI 138 CATCTATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 412 CAGCTG C[AC]G^C[GT]G
MwoI 336 GCCCGAGAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NspI 483 ACATGC [GA]CATG^[CT]
PvuII 412 CAGCTG CAG^CTG
RsaI 22 GTAC GT^AC
StuI 68 AGGCCT AGG^CCT
StyI 31 CCTAGG C^C[AT][AT]GG
TaqI 74 TCGA T^CGA
Tru9I 452 TTAA T^TAA
TseI 414 GCTGC G^C[AT]GC
TthHB8I 74 TCGA T^CGA
XmnI 359 GAAATGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AocI 422 CCTCAGG CC^T[ACGT]AGG
547 CCTCAGG
AvaII 466 GGACC G^G[AT]CC
491 GGACC
BfaI 32 CTAG C^TAG
181 CTAG
BsmAI 8 GAGAC GTCTC(1/5)
27 GAGAC
Bsu36I 422 CCTCAGG CC^T[ACGT]AGG
547 CCTCAGG
Cac8I 305 GCAGGC GC[ACGT]^[ACGT]GC
345 GCCAGC
DpnII 330 GATC ^GATC
537 GATC
EarI 153 GAAGAG CTCTTC(1/4)
532 GAAGAG
Eco81I 422 CCTCAGG CC^T[ACGT]AGG
547 CCTCAGG
Ksp632I 153 GAAGAG CTCTTC(1/4)
532 GAAGAG
MaeI 32 CTAG C^TAG
181 CTAG
MboI 330 GATC ^GATC
537 GATC
NdeII 330 GATC ^GATC
537 GATC
Sau3AI 330 GATC ^GATC
537 GATC
#########################################
# 3 OR MORE CUT ENZYMES ( 19 enzymes) #
#########################################
BsaJI BsiYI BslI BssKI BstNI
Cfr13I DdeI EcoRII HaeIII HinfI
MboII MnlI MvaI NlaIII NlaIV
Sau96I ScrFI SfaNI TfiI