Restriction map for
pFN21AB6791
##################################
# NO CUT ENZYMES ( 194 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AvaI AviII AvrII
BalI BamHI BanI BbeI BbrPI
BbvCI BbvI BcgI BciVI BclI
BcnI BfaI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BsaI BsaJI BsaWI BseAI
BseRI BsiEI BsiWI BsiYI BslI
BsmBI BsmFI BsmI BsoBI Bsp1407I
BspDI BspEI BspLU11I BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstYI BstZ17I Bsu36I Cac8I
CelII CfoI Cfr10I ClaI CpoI
DdeI DraI DraII DraIII DrdI
DsaI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoT14I EcoT22I FauI FbaI
Fnu4HI FseI FspI HaeII HaeIII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HpaI ItaI KasI
KpnI KspI MaeI MaeII MamI
MfeI MflI MluI MluNI MroI
MscI MseI MslI MunI MvnI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflMI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RsrII SacII SalI SbfI
ScaI SexAI SfaNI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI TaqI Tru9I
TseI TthHB8I Van91I XbaI XcmI
XhoI XhoII XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AciI 158 CCGC CCGC(-3/-1)
AcsI 184 GAATTC [GA]^AATT[CT]
AfaI 18 GTAC GT^AC
AlwI 174 GATCC GGATC(4/5)
ApoI 184 GAATTC [GA]^AATT[CT]
Asp700I 135 GAAGCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 79 GAGCTC G[AT]GC[AT]^C
AspI 349 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 164 GGTCC G^G[AT]CC
BanII 79 GAGCTC G[GA]GC[CT]^C
BbsI 355 GTCTTC GAAGAC(2/6)
BmyI 79 GAGCTC G[AGT]GC[ACT]^C
BpmI 211 CTGGAG CTGGAG(16/14)
BpuAI 355 GTCTTC GAAGAC(2/6)
BsgI 124 GTGCAG GTGCAG(16/14)
BsiHKAI 79 GAGCTC G[AT]GC[AT]^C
BsmAI 214 GAGAC GTCTC(1/5)
Bsp1286I 79 GAGCTC G[AGT]GC[ACT]^C
BspHI 53 TCATGA T^CATGA
BsrI 270 ACTGG ACTGG(1/-1)
BssKI 33 CCAGG ^CC[ACGT]GG
BstF5I 84 CATCC GGATG(2/0)
BstNI 33 CCAGG CC^[AT]GG
BstXI 335 CCATGACATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr13I 164 GGTCC G^G[ACGT]CC
DpnII 174 GATC ^GATC
EarI 76 GAAGAG CTCTTC(1/4)
EcoRI 184 GAATTC G^AATTC
EcoRII 33 CCAGG ^CC[AT]GG
EcoRV 100 GATATC GAT^ATC
FokI 84 CATCC GGATG(9/13)
HinfI 365 GAATC G^A[ACGT]TC
Ksp632I 76 GAAGAG CTCTTC(1/4)
MboI 174 GATC ^GATC
MnlI 199 GAGG CCTC(7/6)
MspA1I 158 CCGCTG C[AC]G^C[GT]G
MvaI 33 CCAGG CC^[AT]GG
MwoI 72 GCCGGAAGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeII 174 GATC ^GATC
NlaIV 163 GGGTCC GG[ACGT]^[ACGT]CC
PflFI 349 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 25 CTGCAG CTGCA^G
RcaI 53 TCATGA T^CATGA
RsaI 18 GTAC GT^AC
SacI 79 GAGCTC GAGCT^C
SapI 76 GAAGAGC GCTCTTC(1/4)
Sau3AI 174 GATC ^GATC
Sau96I 164 GGTCC G^G[ACGT]CC
ScrFI 33 CCAGG CC^[ACGT]GG
SfcI 25 CTGCAG C^T[GA][CT]AG
TfiI 365 GAATC G^A[AT]TC
TspRI 242 CAGTG CA[GC]TG[ACGT][ACGT]^
Tth111I 349 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 135 GAAGCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 7 enzymes) #
###################################
AluI 80 AGCT AG^CT
209 AGCT
HapII 11 CCGG C^CGG
73 CCGG
HpaII 11 CCGG C^CGG
73 CCGG
MboII 76 GAAGA GAAGA(8/7)
356 TCTTC
MspI 11 CCGG C^CGG
73 CCGG
NlaIII 54 CATG CATG^
336 CATG
Tsp509I 185 AATT ^AATT
378 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 3 enzymes) #
#########################################
HphI MaeIII Tsp45I