Restriction map for
pFN21AB0974
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# NO CUT ENZYMES ( 122 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AgeI AhdI Alw44I ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AvaI AviII AvrII BalI
BbrPI BciVI BclI BfaI BfrI
BlnI BlpI Bpu1102I BsaAI BsaBI
BseAI BsiEI BsiWI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstZ17I CelII ClaI CpoI
DraI DraIII DrdI EaeI EagI
Eam1105I EclXI Eco52I EcoO65I EcoRI
EcoRV FbaI FseI FspI HindIII
HphI KpnI KspI MaeI MamI
MfeI MluI MluNI MroI MscI
MunI NaeI NcoI NdeI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SspBI SspI StuI
SwaI TfiI Tth111I XbaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 58 enzymes) #
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AcsI 813 AAATTC [GA]^AATT[CT]
AcyI 1039 GGCGCC G[GA]^CG[CT]C
AfaI 1062 GTAC GT^AC
AflIII 170 ACACGT A^C[GA][CT]GT
AlwNI 58 CAGGCGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 486 CCTCAGG CC^T[ACGT]AGG
Aor51HI 1278 AGCGCT AGC^GCT
ApoI 813 AAATTC [GA]^AATT[CT]
Asp700I 998 GAACCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 385 GGATCC G^GATCC
BbeI 1039 GGCGCC GGCGC^C
BbsI 822 GAAGAC GAAGAC(2/6)
BbvCI 1228 GCTGAGG CCTCAGC(-5/-2)
BglI 1031 GCCCCAGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 446 AGATCT A^GATCT
BpuAI 822 GAAGAC GAAGAC(2/6)
BsaHI 1039 GGCGCC G[GA]^CG[CT]C
BsaI 962 GAGACC GGTCTC(1/5)
BsaWI 266 ACCGGA [AT]^CCGG[AT]
BsmBI 736 GAGACG CGTCTC(1/5)
BsmI 1103 GCATTC GAATGC(1/-1)
BsrBI 189 GAGCGG CCGCTC(-3/-3)
BsrDI 120 GCAATG GCAATG(2/0)
BsrFI 965 ACCGGC [GA]^CCGG[CT]
BstAPI 57 GCAGGCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 1276 CCAGCGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 486 CCTCAGG CC^T[ACGT]AGG
Cfr10I 965 ACCGGC [GA]^CCGG[CT]
DsaI 587 CCGTGG C^C[GA][CT]GG
EarI 442 GAAGAG CTCTTC(1/4)
Eco47III 1278 AGCGCT AGC^GCT
Eco81I 486 CCTCAGG CC^T[ACGT]AGG
EcoT22I 534 ATGCAT ATGCA^T
Hin1I 1039 GGCGCC G[GA]^CG[CT]C
HincII 340 GTTAAC GT[CT]^[GA]AC
HindII 340 GTTAAC GT[CT]^[GA]AC
HpaI 340 GTTAAC GTT^AAC
KasI 1039 GGCGCC G^GCGCC
Ksp632I 442 GAAGAG CTCTTC(1/4)
MaeII 172 ACGT A^CGT
MaeIII 256 GTCAC ^GT[ACGT]AC
MseI 341 TTAA T^TAA
NarI 1039 GGCGCC GG^CGCC
NsiI 534 ATGCAT ATGCA^T
PflMI 680 CCACTGATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 792 CTGCAG CTGCA^G
PvuII 141 CAGCTG CAG^CTG
RsaI 1062 GTAC GT^AC
SfcI 792 CTGCAG C^T[GA][CT]AG
SfoI 1039 GGCGCC GGC^GCC
SphI 536 GCATGC GCATG^C
TaqI 697 TCGA T^CGA
Tru9I 341 TTAA T^TAA
Tsp45I 256 GTCAC ^GT[GC]AC
TthHB8I 697 TCGA T^CGA
Van91I 680 CCACTGATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1276 CCAGCGCTGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 998 GAACCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 12 enzymes) #
###################################
AspHI 211 GTGCTC G[AT]GC[AT]^C
262 GAGCAC
BcgI 1021 CGAATGACCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
329 GCAACAACCTCG
BcnI 116 CCCGG CC^[GC]GG
277 CCCGG
BpmI 427 CTGGAG CTGGAG(16/14)
1237 CTGGAG
BsiHKAI 211 GTGCTC G[AT]GC[AT]^C
262 GAGCAC
EcoNI 952 CCTGTCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1028 CCTGCCCCAGG
FauI 251 CCCGC CCCGC(4/6)
351 CCCGC
HgaI 738 GACGC GACGC(5/10)
254 GCGTC
MspA1I 141 CAGCTG C[AC]G^C[GT]G
150 CAGCGG
NciI 116 CCCGG CC^[GC]GG
277 CCCGG
NspI 90 GCATGT [GA]CATG^[CT]
536 GCATGC
Tsp509I 579 AATT ^AATT
814 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 66 enzymes) #
#########################################
AccII AciI AluI AlwI ApaI
AvaII BanI BanII BbvI BmyI
BsaJI BseRI BsgI BsiYI BslI
BsmAI BsmFI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII ItaI MboI
MboII MflI MnlI MslI MspI
MvaI MvnI MwoI NdeII NlaIII
NlaIV PleI Sau3AI Sau96I ScrFI
SfaNI SmlI StyI TseI TspRI
XhoII