(Circular) (Six-base) MAPSORT of: pFN21AB0974.seq Check: 3607 from: 1 to: 6016 pFN21AB0974 6016 bp With 182 enzymes: SgfI * April 15, 2010 09:33 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2719 2719 Size: 6016 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3826 278 Size: 53 83 186 936 2290 2468 Fragments arranged by size: 2468 2290 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3359 1937 Size: 1422 4594 Acc65I G'GTAC_C Cuts at: 3337 3337 Size: 6016 AclI AA'CG_TT Cuts at: 1563 4143 4516 1563 Size: 2580 373 3063 Fragments arranged by size: 3063 2580 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4079 5463 5731 1632 Size: 2447 1384 268 1917 Fragments arranged by size: 2447 1917 1384 268 AfeI AGC'GCT Cuts at: 3285 3285 Size: 6016 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5819 AflIII A'CryG_T Cuts at: 1203 2175 4915 5892 1203 Size: 972 2740 977 1327 Fragments arranged by size: 2740 1327 977 972 AgeI A'CCGG_T Cuts at: 4847 4847 Size: 6016 AhdI GACnn_n'nnGTC Cuts at: 4745 4745 Size: 6016 AleI CACnn'nnGTG Cuts at: 1166 2309 1166 Size: 1143 4873 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2942 3222 1184 Size: 1758 280 3978 Fragments arranged by size: 3978 1758 280 AlwNI CAG_nnn'CTG Cuts at: 2068 3759 5331 2068 Size: 1691 1572 2753 Fragments arranged by size: 2753 1691 1572 ApaI G_GGCC'C Cuts at: 2282 2368 2592 2282 Size: 86 224 5706 Fragments arranged by size: 5706 224 86 ApaLI G'TGCA_C Cuts at: 4073 5229 4073 Size: 1156 4860 ApoI r'AATT_y Cuts at: 1493 2818 3325 3551 3703 5812 1493 Size: 1325 507 226 152 2109 1697 Fragments arranged by size: 2109 1697 1325 507 226 152 AseI AT'TA_AT Cuts at: 160 1032 4570 160 Size: 872 3538 1606 Fragments arranged by size: 3538 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6016 AvaI C'yCGr_G Cuts at: 1870 1943 3341 1870 Size: 73 1398 4545 Fragments arranged by size: 4545 1398 73 BamHI G'GATC_C Cuts at: 2390 3346 3713 2390 Size: 956 367 4693 Fragments arranged by size: 4693 956 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2166 2400 3044 3337 4792 Size: 325 217 627 379 234 644 293 1455 Cuts at: 4792 618 Size: 1842 Fragments arranged by size: 1842 1455 644 627 379 325 293 234 217 BanII G_rGCy'C Cuts at: 1391 2256 2282 2368 2592 2815 3335 1391 Size: 865 26 86 224 223 520 4072 Fragments arranged by size: 4072 865 520 224 223 86 26 BbeI G_GCGC'C Cuts at: 3048 3048 Size: 6016 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2834 928 Size: 971 935 4110 Fragments arranged by size: 4110 971 935 BbvCI CC'TCA_GC Cuts at: 3234 3234 Size: 6016 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2323 2357 3015 3049 4207 4241 1903 Size: 34 386 34 658 34 1158 34 3678 Fragments arranged by size: 3678 1158 658 386 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3908 5118 3908 Size: 1210 4806 BclI T'GATC_A Cuts at: 1548 1548 Size: 6016 BfrBI ATG'CAT Cuts at: 2541 2541 Size: 6016 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3042 4627 136 Size: 107 122 71 631 605 1370 1585 1525 Fragments arranged by size: 1585 1525 1370 631 605 122 107 71 BglII A'GATC_T Cuts at: 2451 6011 2451 Size: 3560 2456 BlpI GC'TnA_GC Cuts at: 3433 3433 Size: 6016 Bme1580I G_kGCm'C Cuts at: 1790 2282 2368 2405 2592 4077 5233 1790 Size: 492 86 37 187 1485 1156 2573 Fragments arranged by size: 2573 1485 1156 492 187 86 37 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6016 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2399 3747 4705 Size: 416 53 83 399 24 982 1348 958 Cuts at: 4705 442 Size: 1753 Fragments arranged by size: 1753 1348 982 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6016 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2453 3263 4676 1239 Size: 1214 810 1413 2579 Fragments arranged by size: 2579 1413 1214 810 Bpu10I CC'TnA_GC Cuts at: 2498 3234 2498 Size: 736 5280 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2416 2661 2931 4141 5006 5304 2416 Size: 245 270 1210 865 298 3128 Fragments arranged by size: 3128 1210 865 298 270 245 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2961 4679 882 Size: 617 1462 1718 2219 Fragments arranged by size: 2219 1718 1462 617 BsaAI yAC'GTr Cuts at: 493 1204 3807 493 Size: 711 2603 2702 Fragments arranged by size: 2702 2603 711 BsaBI GATnn'nnATC Cuts at: 6010 6010 Size: 6016 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3045 3823 4205 4840 Size: 53 83 186 936 1512 778 382 635 Cuts at: 4840 275 Size: 1451 Fragments arranged by size: 1512 1451 936 778 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2271 4449 4847 5121 5268 5679 2271 Size: 2178 398 274 147 411 2608 Fragments arranged by size: 2608 2178 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2870 2900 1178 Size: 30 165 30 1467 30 4294 Fragments arranged by size: 4294 1467 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2480 2520 2878 2934 1186 Size: 1294 40 358 56 4268 Fragments arranged by size: 4268 1294 358 56 40 BseYI C'CCAG_C Cuts at: 2377 2547 3280 5219 5715 2377 Size: 170 733 1939 496 2678 Fragments arranged by size: 2678 1939 733 496 170 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2081 2215 3011 3218 1163 Size: 573 345 134 796 207 3961 Fragments arranged by size: 3961 796 573 345 207 134 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3466 4227 5255 1540 Size: 275 186 1465 761 1028 2301 Fragments arranged by size: 2301 1465 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2220 2271 4077 4162 5233 2220 Size: 51 1806 85 1071 3003 Fragments arranged by size: 3003 1806 1071 85 51 BsmI GAATG_Cn' Cuts at: 3108 3527 3620 3108 Size: 419 93 5504 Fragments arranged by size: 5504 419 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2735 2735 Size: 6016 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2220 2256 2271 2282 2368 2405 2592 Size: 399 430 36 15 11 86 37 187 Cuts at: 2592 2815 3335 4077 4162 5233 1391 Size: 223 520 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 520 430 399 223 187 86 85 37 36 15 11 BspEI T'CCGG_A Cuts at: 5679 5679 Size: 6016 BspHI T'CATG_A Cuts at: 3905 5635 3905 Size: 1730 4286 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2124 2571 2719 3041 3371 844 Size: 1280 447 148 322 330 3489 Fragments arranged by size: 3489 1280 447 330 322 148 BsrBI CCG'CTC Cuts at: 1394 1823 2196 3903 1394 Size: 429 373 1707 3507 Fragments arranged by size: 3507 1707 429 373 BsrDI GCAATG_nn' Cuts at: 65 1248 2132 4511 4685 65 Size: 1183 884 2379 174 1396 Fragments arranged by size: 2379 1396 1183 884 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2970 4660 4847 1700 Size: 110 1160 1690 187 2869 Fragments arranged by size: 2869 1690 1160 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6016 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6016 BssSI C'ACGA_G Cuts at: 4076 5088 4076 Size: 1012 5004 BstAPI GCAn_nnn'nTGC Cuts at: 794 2068 794 Size: 1274 4742 BstBI TT'CG_AA Cuts at: 4820 4820 Size: 6016 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6016 BstXI CCAn_nnnn'nTGG Cuts at: 3288 3288 Size: 6016 BstYI r'GATC_y Cuts at: 1972 2390 2451 3168 3346 3713 4098 4115 5567 Size: 418 61 717 178 367 385 17 1452 Cuts at: 5567 5653 5665 6011 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 717 418 385 367 346 178 86 61 17 12 Bsu36I CC'TnA_GG Cuts at: 2492 2492 Size: 6016 BtgI C'CryG_G Cuts at: 513 1065 2592 513 Size: 552 1527 3937 Fragments arranged by size: 3937 1527 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5277 Fragments arranged by size: 5277 683 56 BtsI GCAGTG_nn' Cuts at: 2048 2981 3532 4326 4346 2048 Size: 933 551 794 20 3718 Fragments arranged by size: 3718 933 794 551 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6016 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2763 2798 529 Size: 35 2199 35 3747 Fragments arranged by size: 3747 2199 35 35 DraI TTT'AAA Cuts at: 3320 3320 Size: 6016 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5023 809 Size: 507 3707 1802 Fragments arranged by size: 3707 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3463 4352 5981 Size: 54 1425 50 275 18 1633 889 1629 Cuts at: 5981 8 Size: 43 Fragments arranged by size: 1633 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3463 1537 Size: 275 1651 4090 Fragments arranged by size: 4090 1651 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2442 3946 1353 Size: 1089 1504 3423 Fragments arranged by size: 3423 1504 1089 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4603 4977 5123 387 Size: 1064 138 3014 374 146 1280 Fragments arranged by size: 3014 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2453 3263 4079 4676 5463 5731 1239 Size: 393 821 810 816 597 787 268 1524 Fragments arranged by size: 1524 821 816 810 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2961 3037 2961 Size: 76 5940 EcoO109I rG'GnC_Cy Cuts at: 2279 2365 3238 2279 Size: 86 873 5057 Fragments arranged by size: 5057 873 86 EcoRI G'AATT_C Cuts at: 3325 3703 3325 Size: 378 5638 FspI TGC'GCA Cuts at: 4522 4522 Size: 6016 HaeII r_GCGC'y Cuts at: 1678 2069 3048 3287 5163 1678 Size: 391 979 239 1876 2531 Fragments arranged by size: 2531 1876 979 391 239 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2375 2407 2496 2528 2623 Size: 32 135 32 803 32 89 32 95 Cuts at: 2623 2655 4657 4689 4731 4763 1373 Size: 32 2002 32 42 32 2626 Fragments arranged by size: 2626 2002 803 135 95 89 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2347 3360 3606 669 Size: 988 281 409 1013 246 3079 Fragments arranged by size: 3079 1013 988 409 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6016 HpaI GTT'AAC Cuts at: 2347 3606 2347 Size: 1259 4757 KasI G'GCGC_C Cuts at: 3044 3044 Size: 6016 KpnI G_GTAC'C Cuts at: 3341 3341 Size: 6016 MfeI C'AATT_G Cuts at: 3615 3615 Size: 6016 MluI A'CGCG_T Cuts at: 5892 5892 Size: 6016 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2136 5130 5314 1305 Size: 831 2994 184 2007 Fragments arranged by size: 2994 2007 831 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4194 Fragments arranged by size: 4194 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2180 2309 2478 2654 3057 3101 3974 Size: 648 1014 129 169 176 403 44 873 Cuts at: 3974 4333 4492 518 Size: 359 159 2042 Fragments arranged by size: 2042 1014 873 648 403 359 176 169 159 129 44 MspA1I CmG'CkG Cuts at: 1606 2148 2157 3432 4109 5257 5502 1606 Size: 542 9 1275 677 1148 245 2120 Fragments arranged by size: 2120 1275 1148 677 542 245 9 NarI GG'CG_CC Cuts at: 3045 3045 Size: 6016 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5464 NdeI CA'TA_TG Cuts at: 387 387 Size: 6016 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6016 NotI GC'GGCC_GC Cuts at: 3463 3463 Size: 6016 NsiI A_TGCA'T Cuts at: 2543 2543 Size: 6016 NspI r_CATG'y Cuts at: 1132 1229 2099 2545 3374 4919 1132 Size: 97 870 446 829 1545 2229 Fragments arranged by size: 2229 1545 870 829 446 97 PasI CC'CwG_GG Cuts at: 1348 3039 1348 Size: 1691 4325 PciI A'CATG_T Cuts at: 4915 4915 Size: 6016 PflMI CCAn_nnn'nTGG Cuts at: 1108 2691 1108 Size: 1583 4433 PmeI GTTT'AAAC Cuts at: 3320 3320 Size: 6016 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4085 4117 5598 5630 4085 Size: 32 1481 32 4471 Fragments arranged by size: 4471 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6016 PsiI TTA'TAA Cuts at: 3586 3586 Size: 6016 PspOMI G'GGCC_C Cuts at: 2278 2364 2588 2278 Size: 86 224 5706 Fragments arranged by size: 5706 224 86 PstI C_TGCA'G Cuts at: 830 2801 3368 830 Size: 1971 567 3478 Fragments arranged by size: 3478 1971 567 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6016 PvuII CAG'CTG Cuts at: 2148 2148 Size: 6016 SalI G'TCGA_C Cuts at: 1936 3358 1936 Size: 1422 4594 SbfI CC_TGCA'GG Cuts at: 3368 3368 Size: 6016 ScaI AGT'ACT Cuts at: 4264 5976 4264 Size: 1712 4304 SfcI C'TryA_G Cuts at: 826 1045 2797 3364 4499 5180 5371 826 Size: 219 1752 567 1135 681 191 1471 Fragments arranged by size: 1752 1471 1135 681 567 219 191 SfoI GGC'GCC Cuts at: 3046 3046 Size: 6016 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6016 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6016 SmaI CCC'GGG Cuts at: 1872 3343 1872 Size: 1471 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2395 2676 2946 4120 5021 5283 Size: 197 926 452 281 270 1174 901 262 Cuts at: 5283 820 Size: 1553 Fragments arranged by size: 1553 1174 926 901 452 281 270 262 197 SnaBI TAC'GTA Cuts at: 493 3807 493 Size: 3314 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6016 SphI G_CATG'C Cuts at: 1132 2545 3374 1132 Size: 1413 829 3774 Fragments arranged by size: 3774 1413 829 SspI AAT'ATT Cuts at: 5 52 3940 5 Size: 47 3888 2081 Fragments arranged by size: 3888 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6016 StyI C'CwwG_G Cuts at: 513 1065 2223 2241 2460 2998 3455 513 Size: 552 1158 18 219 538 457 3074 Fragments arranged by size: 3074 1158 552 538 457 219 18 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4043 4060 4213 4398 4843 325 Size: 1255 2463 17 153 185 445 1498 Fragments arranged by size: 2463 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4262 5974 96 Size: 274 80 33 51 1443 2285 1712 138 Fragments arranged by size: 2285 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2190 2222 2870 2902 5976 6008 2190 Size: 32 648 32 3074 32 2198 Fragments arranged by size: 3074 2198 648 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6016 XbaI T'CTAG_A Cuts at: 3352 3352 Size: 6016 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3288 3288 Size: 6016 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6016 XmaI C'CCGG_G Cuts at: 1870 3341 1870 Size: 1471 4545 XmnI GAAnn'nnTTC Cuts at: 3007 4145 3007 Size: 1138 4878 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3824 276 Size: 53 83 186 936 2290 2468 Fragments arranged by size: 2468 2290 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BplI BsiWI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI NaeI NgoMIV NruI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI