Restriction map for
pFN21AB0974
(Linear) (Six-base) MAPSORT of: KIBB0974.seq Check: 729 from: 1 to: 1311
KIBB0974.orf
With 182 enzymes: SgfI *
October 7, 2009 14:15 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 714 1311
Size: 714 597
AfeI AGC'GCT
Cuts at: 0 1280 1311
Size: 1280 31
AflIII A'CryG_T
Cuts at: 0 170 1311
Size: 170 1141
AleI CACnn'nnGTG
Cuts at: 0 304 1311
Size: 304 1007
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 937 1217 1311
Size: 937 280 94
Fragments arranged by size:
937 280 94
AlwNI CAG_nnn'CTG
Cuts at: 0 63 1311
Size: 63 1248
ApaI G_GGCC'C
Cuts at: 0 277 363 587 1311
Size: 277 86 224 724
Fragments arranged by size:
724 277 224 86
ApoI r'AATT_y
Cuts at: 0 813 1311
Size: 813 498
BamHI G'GATC_C
Cuts at: 0 385 1311
Size: 385 926
BanI G'GyrC_C
Cuts at: 0 161 395 1039 1311
Size: 161 234 644 272
Fragments arranged by size:
644 272 234 161
BanII G_rGCy'C
Cuts at: 0 251 277 363 587 810 1311
Size: 251 26 86 224 223 501
Fragments arranged by size:
501 251 224 223 86 26
BbeI G_GCGC'C
Cuts at: 0 1043 1311
Size: 1043 268
BbsI GAAGACnn'nnnn_
Cuts at: 0 829 1311
Size: 829 482
BbvCI CC'TCA_GC
Cuts at: 0 1229 1311
Size: 1229 82
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 318 352 1010 1044 1311
Size: 318 34 658 34 267
Fragments arranged by size:
658 318 267 34 34
BfrBI ATG'CAT
Cuts at: 0 536 1311
Size: 536 775
BglI GCCn_nnn'nGGC
Cuts at: 0 1037 1311
Size: 1037 274
BglII A'GATC_T
Cuts at: 0 446 1311
Size: 446 865
Bme1580I G_kGCm'C
Cuts at: 0 277 363 400 587 1311
Size: 277 86 37 187 724
Fragments arranged by size:
724 277 187 86 37
BmrI ACTGGGnnnn_n'
Cuts at: 0 394 1311
Size: 394 917
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 448 1258 1311
Size: 448 810 53
Fragments arranged by size:
810 448 53
Bpu10I CC'TnA_GC
Cuts at: 0 493 1229 1311
Size: 493 736 82
Fragments arranged by size:
736 493 82
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 411 656 926 1311
Size: 411 245 270 385
Fragments arranged by size:
411 385 270 245
BsaI GGTCTCn'nnnn_
Cuts at: 0 956 1311
Size: 956 355
BsaHI Gr'CG_yC
Cuts at: 0 1040 1311
Size: 1040 271
BsaWI w'CCGG_w
Cuts at: 0 266 1311
Size: 266 1045
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 865 895 1311
Size: 865 30 416
Fragments arranged by size:
865 416 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 475 515 873 929 1311
Size: 475 40 358 56 382
Fragments arranged by size:
475 382 358 56 40
BseYI C'CCAG_C
Cuts at: 0 372 542 1275 1311
Size: 372 170 733 36
Fragments arranged by size:
733 372 170 36
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 76 210 1006 1213 1311
Size: 76 134 796 207 98
Fragments arranged by size:
796 207 134 98 76
BsiHKAI G_wGCw'C
Cuts at: 0 215 266 1311
Size: 215 51 1045
Fragments arranged by size:
1045 215 51
BsmI GAATG_Cn'
Cuts at: 0 1103 1311
Size: 1103 208
BsmBI CGTCTCn'nnnn_
Cuts at: 0 730 1311
Size: 730 581
Bsp1286I G_dGCh'C
Cuts at: 0 215 251 266 277 363 400 587 810
Size: 215 36 15 11 86 37 187 223
Cuts at: 810 1311
Size: 501
Fragments arranged by size:
501 223 215 187 86 37 36 15
11
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 119 566 714 1036 1311
Size: 119 447 148 322 275
Fragments arranged by size:
447 322 275 148 119
BsrBI CCG'CTC
Cuts at: 0 191 1311
Size: 191 1120
BsrDI GCAATG_nn'
Cuts at: 0 127 1311
Size: 127 1184
BsrFI r'CCGG_y
Cuts at: 0 965 1311
Size: 965 346
BstAPI GCAn_nnn'nTGC
Cuts at: 0 63 1311
Size: 63 1248
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1283 1311
Size: 1283 28
BstYI r'GATC_y
Cuts at: 0 385 446 1163 1311
Size: 385 61 717 148
Fragments arranged by size:
717 385 148 61
Bsu36I CC'TnA_GG
Cuts at: 0 487 1311
Size: 487 824
BtgI C'CryG_G
Cuts at: 0 587 1311
Size: 587 724
BtsI GCAGTG_nn'
Cuts at: 0 43 976 1311
Size: 43 933 335
Fragments arranged by size:
933 335 43
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 758 793 1311
Size: 758 35 518
Fragments arranged by size:
758 518 35
EarI CTCTTCn'nnn_
Cuts at: 0 437 1311
Size: 437 874
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 448 1258 1311
Size: 448 810 53
Fragments arranged by size:
810 448 53
EcoNI CCTnn'n_nnAGG
Cuts at: 0 956 1032 1311
Size: 956 76 279
Fragments arranged by size:
956 279 76
EcoO109I rG'GnC_Cy
Cuts at: 0 274 360 1233 1311
Size: 274 86 873 78
Fragments arranged by size:
873 274 86 78
HaeII r_GCGC'y
Cuts at: 0 64 1043 1282 1311
Size: 64 979 239 29
Fragments arranged by size:
979 239 64 29
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 370 402 491 523 618 650 1311
Size: 370 32 89 32 95 32 661
Fragments arranged by size:
661 370 95 89 32 32 32
HincII GTy'rAC
Cuts at: 0 342 1311
Size: 342 969
HpaI GTT'AAC
Cuts at: 0 342 1311
Size: 342 969
KasI G'GCGC_C
Cuts at: 0 1039 1311
Size: 1039 272
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 131 1311
Size: 131 1180
MslI CAynn'nnrTG
Cuts at: 0 175 304 473 649 1052 1096 1311
Size: 175 129 169 176 403 44 215
Fragments arranged by size:
403 215 176 175 169 129 44
MspA1I CmG'CkG
Cuts at: 0 143 152 1311
Size: 143 9 1159
Fragments arranged by size:
1159 143 9
NarI GG'CG_CC
Cuts at: 0 1040 1311
Size: 1040 271
NsiI A_TGCA'T
Cuts at: 0 538 1311
Size: 538 773
NspI r_CATG'y
Cuts at: 0 94 540 1311
Size: 94 446 771
Fragments arranged by size:
771 446 94
PasI CC'CwG_GG
Cuts at: 0 1034 1311
Size: 1034 277
PflMI CCAn_nnn'nTGG
Cuts at: 0 686 1311
Size: 686 625
PspOMI G'GGCC_C
Cuts at: 0 273 359 583 1311
Size: 273 86 224 728
Fragments arranged by size:
728 273 224 86
PstI C_TGCA'G
Cuts at: 0 796 1311
Size: 796 515
PvuII CAG'CTG
Cuts at: 0 143 1311
Size: 143 1168
SfcI C'TryA_G
Cuts at: 0 792 1311
Size: 792 519
SfoI GGC'GCC
Cuts at: 0 1041 1311
Size: 1041 270
SmlI C'TyrA_G
Cuts at: 0 390 671 941 1311
Size: 390 281 270 370
Fragments arranged by size:
390 370 281 270
SphI G_CATG'C
Cuts at: 0 540 1311
Size: 540 771
StyI C'CwwG_G
Cuts at: 0 218 236 455 993 1311
Size: 218 18 219 538 318
Fragments arranged by size:
538 318 219 218 18
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 185 217 865 897 1311
Size: 185 32 648 32 414
Fragments arranged by size:
648 414 185 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1283 1311
Size: 1283 28
XmnI GAAnn'nnTTC
Cuts at: 0 1002 1311
Size: 1002 309
Enzymes that do cut:
AarI AfeI AflIII AleI AlfI AlwNI ApaI ApoI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BfrBI
BglI BglII Bme1580I BmrI BpmI Bpu10I BpuEI BsaI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI
BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI BstAPI BstXI
BstYI Bsu36I BtgI BtsI CspCI EarI Eco57MI EcoNI
EcoO109I HaeII Hin4I HincII HpaI KasI MmeI MslI
MspA1I NarI NsiI NspI PasI PflMI PspOMI PstI
PvuII SfcI SfoI SmlI SphI StyI TstI XcmI
XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AflII AgeI AhdI
AloI ApaLI AscI AseI AsiSI AvaI AvrII BaeI
BciVI BclI BlpI BmgBI BmtI BplI BsaAI BsaBI
BsiEI BsiWI BspEI BspHI BsrGI BssHII BssSI BstBI
BstEII BstZ17I BtgZI ClaI DraI DraIII DrdI EaeI
EagI EciI EcoICRI EcoRI EcoRV FalI FseI FspI
FspAI HindIII KpnI MfeI MluI MscI NaeI NcoI
NdeI NgoMIV NheI NotI NruI PacI PciI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI
PvuI RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI
SrfI SspI StuI SwaI TaqII TatI Tth111I XbaI
XhoI XmaI ZraI