Restriction map for
pFN21AB0973
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# NO CUT ENZYMES ( 152 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AvrII BalI BamHI BbrPI BbsI
BbvCI BciVI BclI BfrI BlnI
BlpI BpmI Bpu1102I BpuAI BsaBI
BsaWI BseAI BsgI BsiEI BsiHKAI
BsiWI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstXI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraI DraIII
EaeI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoRI
EcoRV EcoT22I FbaI FseI HincII
HindII HindIII HinfI HpaI KpnI
Ksp632I KspI MamI MboII MfeI
MluI MluNI MroI MscI MseI
MslI MunI NaeI NcoI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfcI
SfuI SgrAI SnaBI SpeI Sse8387I
SspBI SspI SwaI TfiI Tru9I
Tsp509I Tth111I Van91I XbaI XcmI
XhoI XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccII 45 CGCG CG^CG
AcyI 545 GGCGCC G[GA]^CG[CT]C
AlwI 483 GATCC GGATC(4/5)
AvaI 370 CCCGGG C^[CT]CG[GA]G
AviII 53 TGCGCA TGC^GCA
BanI 545 GGCGCC G^G[CT][GA]CC
BbeI 545 GGCGCC GGCGC^C
BcgI 34 CGAGGCCGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 496 CTAG C^TAG
BglI 574 GCCTTCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 443 AGATCT A^GATCT
BsaAI 595 TACGTG [CT]AC^GT[GA]
BsaHI 545 GGCGCC G[GA]^CG[CT]C
BseRI 91 GAGGAG GAGGAG(10/8)
BsmBI 109 GAGACG CGTCTC(1/5)
BsmFI 402 GTCCC GGGAC(10/14)
BsoBI 370 CCCGGG C^[CT]CG[GA]G
BspMI 464 ACCTGC ACCTGC(4/8)
BstEII 99 GGTGACC G^GT[ACGT]ACC
BstPI 99 GGTGACC G^GT[ACGT]ACC
BstUI 45 CGCG CG^CG
BstYI 443 AGATCT [GA]^GATC[CT]
DrdI 393 GACCCTGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 99 GGTGACC G^GT[ACGT]ACC
EcoT14I 228 CCTTGG C^C[AT][AT]GG
FauI 340 GCGGG CCCGC(4/6)
FspI 53 TGCGCA TGC^GCA
HaeII 545 GGCGCC [GA]GCGC^[CT]
HgaI 210 GCGTC GACGC(5/10)
Hin1I 545 GGCGCC G[GA]^CG[CT]C
KasI 545 GGCGCC G^GCGCC
MaeI 496 CTAG C^TAG
MflI 443 AGATCT [GA]^GATC[CT]
MvnI 45 CGCG CG^CG
NarI 545 GGCGCC GG^CGCC
NdeI 536 CATATG CA^TATG
NlaIV 545 GGCGCC GG[ACGT]^[ACGT]CC
NspI 56 GCATGC [GA]CATG^[CT]
SfaNI 514 GATGC GCATC(5/9)
SfiI 573 GGCCTTCCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 545 GGCGCC GGC^GCC
SmaI 370 CCCGGG CCC^GGG
SmlI 568 CTCAAG C^T[CT][GA]AG
SphI 56 GCATGC GCATG^C
StuI 572 AGGCCT AGG^CCT
StyI 228 CCTTGG C^C[AT][AT]GG
TaqI 33 TCGA T^CGA
TthHB8I 33 TCGA T^CGA
XhoII 443 AGATCT [GA]^GATC[CT]
XmaCI 370 CCCGGG C^CCGGG
XmaI 370 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AfaI 189 GTAC GT^AC
594 GTAC
BanII 123 GGGCTC G[GA]GC[CT]^C
232 GGGCTC
BsrI 28 CCAGT ACTGG(1/-1)
192 CCAGT
DraII 293 AGGACCT [GA]G^G[ACGT]CC[CT]
491 AGGTCCT
DsaI 14 CCGTGG C^C[GA][CT]GG
104 CCGTGG
EcoO109I 293 AGGACCT [GA]G^G[ACGT]CC[CT]
491 AGGTCCT
HapII 371 CCGG C^CGG
558 CCGG
HpaII 371 CCGG C^CGG
558 CCGG
MaeII 112 ACGT A^CGT
596 ACGT
MaeIII 100 GTGAC ^GT[ACGT]AC
212 GTCAC
MspA1I 39 CCGCTG C[AC]G^C[GT]G
300 CCGCTG
MspI 371 CCGG C^CGG
558 CCGG
PpuMI 293 AGGACCT [GA]G^G[AT]CC[CT]
491 AGGTCCT
RsaI 189 GTAC GT^AC
594 GTAC
Tsp45I 100 GTGAC ^GT[GC]AC
212 GTCAC
TspRI 365 CACTG CA[GC]TG[ACGT][ACGT]^
552 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
#########################################
AciI AluI AvaII BbvI BcnI
BmyI BsaI BsaJI BsiYI BslI
BsmAI Bsp1286I BssKI BstF5I BstNI
Cac8I CfoI Cfr13I DdeI DpnII
EcoRII Fnu4HI FokI HaeIII HhaI
HinP1I HphI ItaI MboI MnlI
MvaI MwoI NciI NdeII NlaIII
Sau3AI Sau96I ScrFI TseI