Restriction map for
pFN21AB0973
(Linear) (Six-base) MAPSORT of: KIBB0973.seq Check: 9709 from: 1 to: 630
KIBB0973.orf
With 182 enzymes: SgfI *
October 7, 2009 14:15 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 400 630
Size: 400 230
AvaI C'yCGr_G
Cuts at: 0 370 630
Size: 370 260
BanI G'GyrC_C
Cuts at: 0 545 630
Size: 545 85
BanII G_rGCy'C
Cuts at: 0 127 236 630
Size: 127 109 394
Fragments arranged by size:
394 127 109
BbeI G_GCGC'C
Cuts at: 0 549 630
Size: 549 81
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 23 57 630
Size: 23 34 573
Fragments arranged by size:
573 34 23
BglI GCCn_nnn'nGGC
Cuts at: 0 580 630
Size: 580 50
BglII A'GATC_T
Cuts at: 0 443 630
Size: 443 187
Bme1580I G_kGCm'C
Cuts at: 0 558 630
Size: 558 72
BmgBI CAC'GTC
Cuts at: 0 113 630
Size: 113 517
BmrI ACTGGGnnnn_n'
Cuts at: 0 22 630
Size: 22 608
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 553 630
Size: 553 77
BsaI GGTCTCn'nnnn_
Cuts at: 0 170 385 431 630
Size: 170 215 46 199
Fragments arranged by size:
215 199 170 46
BsaAI yAC'GTr
Cuts at: 0 597 630
Size: 597 33
BsaHI Gr'CG_yC
Cuts at: 0 546 630
Size: 546 84
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 430 460 630
Size: 430 30 170
Fragments arranged by size:
430 170 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 106 630
Size: 106 524
BsmBI CGTCTCn'nnnn_
Cuts at: 0 103 630
Size: 103 527
Bsp1286I G_dGCh'C
Cuts at: 0 127 236 558 630
Size: 127 109 322 72
Fragments arranged by size:
322 127 109 72
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 473 630
Size: 473 157
BstEII G'GTnAC_C
Cuts at: 0 99 630
Size: 99 531
BstYI r'GATC_y
Cuts at: 0 443 630
Size: 443 187
BtgI C'CryG_G
Cuts at: 0 14 104 630
Size: 14 90 526
Fragments arranged by size:
526 90 14
BtsI GCAGTG_nn'
Cuts at: 0 364 630
Size: 364 266
DrdI GACnn_nn'nnGTC
Cuts at: 0 399 630
Size: 399 231
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 400 630
Size: 400 230
EcoO109I rG'GnC_Cy
Cuts at: 0 294 492 630
Size: 294 198 138
Fragments arranged by size:
294 198 138
FspI TGC'GCA
Cuts at: 0 55 630
Size: 55 575
HaeII r_GCGC'y
Cuts at: 0 549 630
Size: 549 81
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 428 430 460 462 630
Size: 428 2 30 2 168
Fragments arranged by size:
428 168 30 2 2
KasI G'GCGC_C
Cuts at: 0 545 630
Size: 545 85
MspA1I CmG'CkG
Cuts at: 0 41 302 630
Size: 41 261 328
Fragments arranged by size:
328 261 41
NarI GG'CG_CC
Cuts at: 0 546 630
Size: 546 84
NdeI CA'TA_TG
Cuts at: 0 537 630
Size: 537 93
NspI r_CATG'y
Cuts at: 0 60 630
Size: 60 570
PfoI T'CCnGG_A
Cuts at: 0 167 630
Size: 167 463
PpuMI rG'GwC_Cy
Cuts at: 0 294 492 630
Size: 294 198 138
Fragments arranged by size:
294 198 138
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 580 630
Size: 580 50
SfoI GGC'GCC
Cuts at: 0 547 630
Size: 547 83
SmaI CCC'GGG
Cuts at: 0 372 630
Size: 372 258
SmlI C'TyrA_G
Cuts at: 0 568 630
Size: 568 62
SphI G_CATG'C
Cuts at: 0 60 630
Size: 60 570
SrfI GCCC'GGGC
Cuts at: 0 372 630
Size: 372 258
StuI AGG'CCT
Cuts at: 0 574 630
Size: 574 56
StyI C'CwwG_G
Cuts at: 0 228 630
Size: 228 402
XmaI C'CCGG_G
Cuts at: 0 370 630
Size: 370 260
Enzymes that do cut:
AcuI AvaI BanI BanII BbeI BcgI BglI BglII
Bme1580I BmgBI BmrI BpuEI BsaI BsaAI BsaHI BsaXI
BseRI BsmBI Bsp1286I BspMI BstEII BstYI BtgI BtsI
DrdI Eco57MI EcoO109I FspI HaeII Hin4I KasI MspA1I
NarI NdeI NspI PfoI PpuMI SfiI SfoI SmaI
SmlI SphI SrfI StuI StyI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AscI AseI AsiSI AvrII BaeI BamHI BbsI
BbvCI BciVI BclI BfrBI BlpI BmtI BplI BpmI
Bpu10I BsaBI BsaWI BseYI BsgI BsiEI BsiHKAI BsiWI
BsmI BspEI BspHI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstXI BstZ17I Bsu36I BtgZI ClaI
CspCI DraI DraIII EaeI EagI EarI EciI EcoICRI
EcoNI EcoRI EcoRV FalI FseI FspAI HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI NaeI
NcoI NgoMIV NheI NotI NruI NsiI PacI PasI
PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI
PspXI PsrI PstI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI
SgrAI SnaBI SpeI SspI SwaI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmnI ZraI