(Circular) (Six-base) MAPSORT of: pFN21AB0972.seq Check: 4420 from: 1 to: 6016 pFN21AB0972 6016 bp With 182 enzymes: SgfI * April 15, 2010 09:33 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2591 2859 3166 2591 Size: 268 307 5441 Fragments arranged by size: 5441 307 268 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3826 278 Size: 53 83 186 936 2290 2468 Fragments arranged by size: 2468 2290 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3359 1937 Size: 1422 4594 Acc65I G'GTAC_C Cuts at: 3337 3337 Size: 6016 AclI AA'CG_TT Cuts at: 1563 4143 4516 1563 Size: 2580 373 3063 Fragments arranged by size: 3063 2580 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3012 4079 5463 5731 1632 Size: 1380 1067 1384 268 1917 Fragments arranged by size: 1917 1384 1380 1067 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5819 AflIII A'CryG_T Cuts at: 1203 2025 4915 5892 1203 Size: 822 2890 977 1327 Fragments arranged by size: 2890 1327 977 822 AgeI A'CCGG_T Cuts at: 4847 4847 Size: 6016 AhdI GACnn_n'nnGTC Cuts at: 2959 3187 4745 2959 Size: 228 1558 4230 Fragments arranged by size: 4230 1558 228 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6016 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6016 AlwNI CAG_nnn'CTG Cuts at: 2340 2812 3049 3238 3759 5331 2340 Size: 472 237 189 521 1572 3025 Fragments arranged by size: 3025 1572 521 472 237 189 ApaLI G'TGCA_C Cuts at: 4073 5229 4073 Size: 1156 4860 ApoI r'AATT_y Cuts at: 1493 2147 2159 2415 2879 3325 3551 3703 5812 Size: 654 12 256 464 446 226 152 2109 Cuts at: 5812 1493 Size: 1697 Fragments arranged by size: 2109 1697 654 464 446 256 226 152 12 AseI AT'TA_AT Cuts at: 160 1032 4570 160 Size: 872 3538 1606 Fragments arranged by size: 3538 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6016 AvaI C'yCGr_G Cuts at: 1870 1943 3341 1870 Size: 73 1398 4545 Fragments arranged by size: 4545 1398 73 BamHI G'GATC_C Cuts at: 3211 3346 3713 3211 Size: 135 367 5514 Fragments arranged by size: 5514 367 135 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3337 4792 618 Size: 325 217 627 1550 1455 1842 Fragments arranged by size: 1842 1550 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2086 2826 3335 1391 Size: 695 740 509 4072 Fragments arranged by size: 4072 740 695 509 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2450 3124 928 Size: 971 551 674 3820 Fragments arranged by size: 3820 971 674 551 BbvCI CC'TCA_GC Cuts at: 3059 3059 Size: 6016 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4207 4241 1903 Size: 34 2270 34 3678 Fragments arranged by size: 3678 2270 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2040 3908 5118 2040 Size: 1868 1210 2938 Fragments arranged by size: 2938 1868 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6016 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2054 4627 Size: 107 122 71 631 605 337 45 2573 Cuts at: 4627 136 Size: 1525 Fragments arranged by size: 2573 1525 631 605 337 122 107 71 45 BglII A'GATC_T Cuts at: 3306 6011 3306 Size: 2705 3311 BlpI GC'TnA_GC Cuts at: 2742 2820 3433 2742 Size: 78 613 5325 Fragments arranged by size: 5325 613 78 Bme1580I G_kGCm'C Cuts at: 1790 2328 4077 5233 1790 Size: 538 1749 1156 2573 Fragments arranged by size: 2573 1749 1156 538 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6016 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2593 2641 3747 Size: 416 53 83 399 24 1176 48 1106 Cuts at: 3747 4705 442 Size: 958 1753 Fragments arranged by size: 1753 1176 1106 958 416 399 83 53 48 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6016 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2062 2253 3141 4676 1239 Size: 823 191 888 1535 2579 Fragments arranged by size: 2579 1535 888 823 191 Bpu10I CC'TnA_GC Cuts at: 2085 3059 2085 Size: 974 5042 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4141 5006 5304 4141 Size: 865 298 4853 Fragments arranged by size: 4853 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2666 3221 4679 882 Size: 617 1167 555 1458 2219 Fragments arranged by size: 2219 1458 1167 617 555 BsaAI yAC'GTr Cuts at: 493 1204 2028 3807 493 Size: 711 824 1779 2702 Fragments arranged by size: 2702 1779 824 711 BsaBI GATnn'nnATC Cuts at: 6010 6010 Size: 6016 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3823 4205 4840 275 Size: 53 83 186 936 2290 382 635 1451 Fragments arranged by size: 2290 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4449 4847 5121 5268 5679 4449 Size: 398 274 147 411 4786 Fragments arranged by size: 4786 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3114 3144 1178 Size: 30 165 30 1711 30 4050 Fragments arranged by size: 4050 1711 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2264 2768 2798 2801 3239 1186 Size: 1078 504 30 3 438 3963 Fragments arranged by size: 3963 1078 504 438 30 3 BseYI C'CCAG_C Cuts at: 2012 2179 2329 5219 5715 2012 Size: 167 150 2890 496 2313 Fragments arranged by size: 2890 2313 496 167 150 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2312 2485 1163 Size: 573 576 173 4694 Fragments arranged by size: 4694 576 573 173 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3466 4227 5255 1540 Size: 275 186 1465 761 1028 2301 Fragments arranged by size: 2301 1465 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2100 2943 4077 4162 5233 2100 Size: 843 1134 85 1071 2883 Fragments arranged by size: 2883 1134 1071 843 85 BsmI GAATG_Cn' Cuts at: 2398 3073 3527 3620 2398 Size: 675 454 93 4794 Fragments arranged by size: 4794 675 454 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2086 2100 2328 2826 2943 3335 4077 Size: 399 296 14 228 498 117 392 742 Cuts at: 4077 4162 5233 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 498 399 392 296 228 117 85 14 BspEI T'CCGG_A Cuts at: 5679 5679 Size: 6016 BspHI T'CATG_A Cuts at: 3012 3905 5635 3012 Size: 893 1730 3393 Fragments arranged by size: 3393 1730 893 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2515 2591 2859 3166 3371 844 Size: 1671 76 268 307 205 3489 Fragments arranged by size: 3489 1671 307 268 205 76 BsrBI CCG'CTC Cuts at: 1394 1823 2746 3903 1394 Size: 429 923 1157 3507 Fragments arranged by size: 3507 1157 923 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2226 2251 4511 4685 65 Size: 1183 978 25 2260 174 1396 Fragments arranged by size: 2260 1396 1183 978 174 25 BsrFI r'CCGG_y Cuts at: 1700 1810 2168 4660 4847 1700 Size: 110 358 2492 187 2869 Fragments arranged by size: 2869 2492 358 187 110 BsrGI T'GTAC_A Cuts at: 96 2022 96 Size: 1926 4090 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6016 BssSI C'ACGA_G Cuts at: 4076 5088 4076 Size: 1012 5004 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6016 BstBI TT'CG_AA Cuts at: 4820 4820 Size: 6016 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6016 BstXI CCAn_nnnn'nTGG Cuts at: 2934 2934 Size: 6016 BstYI r'GATC_y Cuts at: 1972 3211 3306 3346 3713 4098 4115 5567 5653 Size: 1239 95 40 367 385 17 1452 86 Cuts at: 5653 5665 6011 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1239 385 367 346 95 86 40 17 12 Bsu36I CC'TnA_GG Cuts at: 2781 2781 Size: 6016 BtgI C'CryG_G Cuts at: 513 1065 2004 2696 513 Size: 552 939 692 3833 Fragments arranged by size: 3833 939 692 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2047 487 Size: 683 56 821 4456 Fragments arranged by size: 4456 821 683 56 BtsI GCAGTG_nn' Cuts at: 2300 2921 3532 4326 4346 2300 Size: 621 611 794 20 3970 Fragments arranged by size: 3970 794 621 611 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6016 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5981 DraI TTT'AAA Cuts at: 3320 3320 Size: 6016 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5023 809 Size: 507 3707 1802 Fragments arranged by size: 3707 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2125 3463 4352 Size: 54 1425 50 275 18 295 1338 889 Cuts at: 4352 5981 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1338 889 295 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3463 1537 Size: 275 1651 4090 Fragments arranged by size: 4090 1651 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2715 3946 1353 Size: 1362 1231 3423 Fragments arranged by size: 3423 1362 1231 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2534 3217 4603 4977 5123 387 Size: 1064 138 945 683 1386 374 146 1280 Fragments arranged by size: 1386 1280 1064 945 683 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2062 2253 3012 3141 4079 4676 5463 Size: 393 430 191 759 129 938 597 787 Cuts at: 5463 5731 1239 Size: 268 1524 Fragments arranged by size: 1524 938 787 759 597 430 393 268 191 129 EcoO109I rG'GnC_Cy Cuts at: 2016 2046 2176 2836 3267 2016 Size: 30 130 660 431 4765 Fragments arranged by size: 4765 660 431 130 30 EcoRI G'AATT_C Cuts at: 2415 3325 3703 2415 Size: 910 378 4728 Fragments arranged by size: 4728 910 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2857 2881 3088 2857 Size: 24 207 5785 Fragments arranged by size: 5785 207 24 FspI TGC'GCA Cuts at: 4522 4522 Size: 6016 HaeII r_GCGC'y Cuts at: 1678 2813 5163 1678 Size: 1135 2350 2531 Fragments arranged by size: 2531 2350 1135 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2897 2929 2945 2977 3007 Size: 32 135 32 1325 32 16 32 30 Cuts at: 3007 3039 3114 3146 3173 3205 4657 4689 4731 Size: 32 75 32 27 32 1452 32 42 Cuts at: 4731 4763 1373 Size: 32 2626 Fragments arranged by size: 2626 1452 1325 135 75 42 32 32 32 32 32 32 32 32 32 30 27 16 HincII GTy'rAC Cuts at: 669 1657 1938 3360 3606 669 Size: 988 281 1422 246 3079 Fragments arranged by size: 3079 1422 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6016 HpaI GTT'AAC Cuts at: 3606 3606 Size: 6016 KpnI G_GTAC'C Cuts at: 3341 3341 Size: 6016 MfeI C'AATT_G Cuts at: 3615 3615 Size: 6016 MluI A'CGCG_T Cuts at: 5892 5892 Size: 6016 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2853 5130 5314 1305 Size: 1548 2277 184 2007 Fragments arranged by size: 2277 2007 1548 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2127 10 Size: 54 1425 343 295 3899 Fragments arranged by size: 3899 1425 343 295 54 MslI CAynn'nnrTG Cuts at: 518 1166 3035 3196 3974 4333 4492 518 Size: 648 1869 161 778 359 159 2042 Fragments arranged by size: 2042 1869 778 648 359 161 159 MspA1I CmG'CkG Cuts at: 1606 2079 2571 3160 3432 4109 5257 5502 1606 Size: 473 492 589 272 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 589 492 473 272 245 NaeI GCC'GGC Cuts at: 2170 2170 Size: 6016 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4525 Fragments arranged by size: 4525 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6016 NgoMIV G'CCGG_C Cuts at: 2168 2168 Size: 6016 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6016 NotI GC'GGCC_GC Cuts at: 3463 3463 Size: 6016 NspI r_CATG'y Cuts at: 1132 1229 3281 3374 4919 1132 Size: 97 2052 93 1545 2229 Fragments arranged by size: 2229 2052 1545 97 93 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6016 PciI A'CATG_T Cuts at: 4915 4915 Size: 6016 PflMI CCAn_nnn'nTGG Cuts at: 1108 2340 2571 3049 3238 3280 1108 Size: 1232 231 478 189 42 3844 Fragments arranged by size: 3844 1232 478 231 189 42 PmeI GTTT'AAAC Cuts at: 3320 3320 Size: 6016 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4085 4117 5598 5630 4085 Size: 32 1481 32 4471 Fragments arranged by size: 4471 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2016 2176 2836 2016 Size: 160 660 5196 Fragments arranged by size: 5196 660 160 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6016 PsiI TTA'TAA Cuts at: 3586 3586 Size: 6016 PstI C_TGCA'G Cuts at: 830 3368 830 Size: 2538 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6016 SalI G'TCGA_C Cuts at: 1936 3358 1936 Size: 1422 4594 SanDI GG'GwC_CC Cuts at: 2016 2016 Size: 6016 SbfI CC_TGCA'GG Cuts at: 3368 3368 Size: 6016 ScaI AGT'ACT Cuts at: 2266 2894 3251 4264 5976 2266 Size: 628 357 1013 1712 2306 Fragments arranged by size: 2306 1712 1013 628 357 SfcI C'TryA_G Cuts at: 826 1045 3364 4499 5180 5371 826 Size: 219 2319 1135 681 191 1471 Fragments arranged by size: 2319 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6016 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6016 SmaI CCC'GGG Cuts at: 1872 3343 1872 Size: 1471 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4120 5021 5283 820 Size: 197 926 2177 901 262 1553 Fragments arranged by size: 2177 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3807 493 Size: 3314 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6016 SphI G_CATG'C Cuts at: 1132 3374 1132 Size: 2242 3774 SspI AAT'ATT Cuts at: 5 52 3940 5 Size: 47 3888 2081 Fragments arranged by size: 3888 2081 47 StuI AGG'CCT Cuts at: 1517 2552 1517 Size: 1035 4981 StyI C'CwwG_G Cuts at: 513 1065 2004 2355 2826 2832 3455 513 Size: 552 939 351 471 6 623 3074 Fragments arranged by size: 3074 939 623 552 471 351 6 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2287 2332 4043 4060 4213 4398 4843 Size: 1255 707 45 1711 17 153 185 445 Cuts at: 4843 325 Size: 1498 Fragments arranged by size: 1711 1498 1255 707 445 185 153 45 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2022 2073 2264 Size: 274 80 33 51 1443 45 51 191 Cuts at: 2264 2892 3249 4262 5974 96 Size: 628 357 1013 1712 138 Fragments arranged by size: 1712 1443 1013 628 357 274 191 138 80 51 51 45 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2100 2132 2193 2225 5976 6008 2100 Size: 32 61 32 3751 32 2108 Fragments arranged by size: 3751 2108 61 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6016 XbaI T'CTAG_A Cuts at: 2530 3352 2530 Size: 822 5194 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2120 2337 2120 Size: 217 5799 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6016 XmaI C'CCGG_G Cuts at: 1870 3341 1870 Size: 1471 4545 XmnI GAAnn'nnTTC Cuts at: 3071 4145 3071 Size: 1074 4942 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3824 276 Size: 53 83 186 936 2290 2468 Fragments arranged by size: 2468 2290 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI