Restriction map for
pFN21AB0970
(Circular) (Six-base) MAPSORT of: pFN21AB0970.seq Check: 485 from: 1 to: 5998
pFN21AB0970 5998 bp
With 182 enzymes: SgfI *
April 15, 2010 09:33 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3808 278
Size: 53 83 186 936 2272 2468
Fragments arranged by size:
2468 2272 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3341 1937
Size: 1404 4594
Acc65I G'GTAC_C
Cuts at: 3319 3319
Size: 5998
AclI AA'CG_TT
Cuts at: 1563 3250 4125 4498 1563
Size: 1687 875 373 3063
Fragments arranged by size:
3063 1687 875 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2745 4061 5445 5713 1632
Size: 1113 1316 1384 268 1917
Fragments arranged by size:
1917 1384 1316 1113 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5801
AflIII A'CryG_T
Cuts at: 1203 4897 5874 1203
Size: 3694 977 1327
Fragments arranged by size:
3694 1327 977
AgeI A'CCGG_T
Cuts at: 4829 4829
Size: 5998
AhdI GACnn_n'nnGTC
Cuts at: 2128 4727 2128
Size: 2599 3399
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5998
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5998
AlwNI CAG_nnn'CTG
Cuts at: 3741 5313 3741
Size: 1572 4426
ApaLI G'TGCA_C
Cuts at: 4055 5211 4055
Size: 1156 4842
ApoI r'AATT_y
Cuts at: 1493 2389 3307 3533 3685 5794 1493
Size: 896 918 226 152 2109 1697
Fragments arranged by size:
2109 1697 918 896 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2196 4552 160
Size: 872 1164 2356 1606
Fragments arranged by size:
2356 1606 1164 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5998
AvaI C'yCGr_G
Cuts at: 1870 1943 3323 1870
Size: 73 1380 4545
Fragments arranged by size:
4545 1380 73
BamHI G'GATC_C
Cuts at: 2534 3328 3695 2534
Size: 794 367 4837
Fragments arranged by size:
4837 794 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2929 3319 4774 618
Size: 325 217 627 1142 390 1455 1842
Fragments arranged by size:
1842 1455 1142 627 390 325 217
BanII G_rGCy'C
Cuts at: 1391 2166 3317 1391
Size: 775 1151 4072
Fragments arranged by size:
4072 1151 775
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2193 3039 928
Size: 971 294 846 3887
Fragments arranged by size:
3887 971 846 294
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4189 4223 1903
Size: 34 2252 34 3678
Fragments arranged by size:
3678 2252 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3890 5100 3890
Size: 1210 4788
BclI T'GATC_A
Cuts at: 1548 2638 3273 1548
Size: 1090 635 4273
Fragments arranged by size:
4273 1090 635
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4609 136
Size: 107 122 71 631 605 2937 1525
Fragments arranged by size:
2937 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5993 5993
Size: 5998
BlpI GC'TnA_GC
Cuts at: 3415 3415
Size: 5998
Bme1580I G_kGCm'C
Cuts at: 1790 2584 4059 5215 1790
Size: 794 1475 1156 2573
Fragments arranged by size:
2573 1475 1156 794
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5998
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3729 4687 442
Size: 416 53 83 399 24 2312 958 1753
Fragments arranged by size:
2312 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5998
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2570 4658 1239
Size: 1331 2088 2579
Fragments arranged by size:
2579 2088 1331
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2166 3241 4123 4988 5286 2166
Size: 1075 882 865 298 2878
Fragments arranged by size:
2878 1075 882 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4661 882
Size: 617 3162 2219
Fragments arranged by size:
3162 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3789 493
Size: 711 2585 2702
Fragments arranged by size:
2702 2585 711
BsaBI GATnn'nnATC
Cuts at: 5992 5992
Size: 5998
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3805 4187 4822 275
Size: 53 83 186 936 2272 382 635 1451
Fragments arranged by size:
2272 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4431 4829 5103 5250 5661 4431
Size: 398 274 147 411 4768
Fragments arranged by size:
4768 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2791 2821 1178
Size: 30 165 30 1388 30 4355
Fragments arranged by size:
4355 1388 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5998
BseYI C'CCAG_C
Cuts at: 2583 2683 2784 5201 5697 2583
Size: 100 101 2417 496 2884
Fragments arranged by size:
2884 2417 496 101 100
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3006 1163
Size: 573 1270 4155
Fragments arranged by size:
4155 1270 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3448 4209 5237 1540
Size: 275 186 1447 761 1028 2301
Fragments arranged by size:
2301 1447 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4059 4144 5215 4059
Size: 85 1071 4842
Fragments arranged by size:
4842 1071 85
BsmI GAATG_Cn'
Cuts at: 3509 3602 3509
Size: 93 5905
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2166 2584 3317 4059 4144 5215 1391
Size: 399 376 418 733 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 733 418 399 376 85
BspEI T'CCGG_A
Cuts at: 5661 5661
Size: 5998
BspHI T'CATG_A
Cuts at: 3887 5617 3887
Size: 1730 4268
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3353 844
Size: 2509 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3885 1394
Size: 429 2062 3507
Fragments arranged by size:
3507 2062 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2164 2898 4493 4667 65
Size: 1183 916 734 1595 174 1396
Fragments arranged by size:
1595 1396 1183 916 734 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4642 4829 1700
Size: 110 2832 187 2869
Fragments arranged by size:
2869 2832 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5998
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5998
BssSI C'ACGA_G
Cuts at: 4058 5070 4058
Size: 1012 4986
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5998
BstBI TT'CG_AA
Cuts at: 4802 4802
Size: 5998
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5998
BstYI r'GATC_y
Cuts at: 1972 2534 3282 3328 3695 4080 4097 5549 5635
Size: 562 748 46 367 385 17 1452 86
Cuts at: 5635 5647 5993 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 748 562 385 367 346 86
46 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5446
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5259
Fragments arranged by size:
5259 683 56
BtsI GCAGTG_nn'
Cuts at: 3121 3514 4308 4328 3121
Size: 393 794 20 4791
Fragments arranged by size:
4791 794 393 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5998
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5963
DraI TTT'AAA
Cuts at: 3302 3302
Size: 5998
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5005 809
Size: 507 3689 1802
Fragments arranged by size:
3689 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2434 2796 3445
Size: 54 1425 50 275 18 604 362 649
Cuts at: 3445 4334 5963 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 649 604 362 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3445 1537
Size: 275 1633 4090
Fragments arranged by size:
4090 1633 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3928 1353
Size: 2575 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4585 4959 5105 387
Size: 1064 138 2996 374 146 1280
Fragments arranged by size:
2996 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2570 2745 4061 4658 5445 5713 1239
Size: 393 938 175 1316 597 787 268 1524
Fragments arranged by size:
1524 1316 938 787 597 393 268 175
EcoO109I rG'GnC_Cy
Cuts at: 3168 3168
Size: 5998
EcoRI G'AATT_C
Cuts at: 3307 3685 3307
Size: 378 5620
EcoRV GAT'ATC
Cuts at: 2500 2500
Size: 5998
FspI TGC'GCA
Cuts at: 4504 4504
Size: 5998
HaeII r_GCGC'y
Cuts at: 1678 5145 1678
Size: 3467 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2114 2146 2360 2392 4639
Size: 32 135 32 542 32 214 32 2247
Cuts at: 4639 4671 4713 4745 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2247 542 214 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3342 3588 669
Size: 988 281 1404 246 3079
Fragments arranged by size:
3079 1404 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5998
HpaI GTT'AAC
Cuts at: 3588 3588
Size: 5998
KpnI G_GTAC'C
Cuts at: 3323 3323
Size: 5998
MfeI C'AATT_G
Cuts at: 3597 3597
Size: 5998
MluI A'CGCG_T
Cuts at: 5874 5874
Size: 5998
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3006 5112 5296 1305
Size: 1701 2106 184 2007
Fragments arranged by size:
2106 2007 1701 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2436 2798 10
Size: 54 1425 343 604 362 3210
Fragments arranged by size:
3210 1425 604 362 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3956 4315 4474 518
Size: 648 2790 359 159 2042
Fragments arranged by size:
2790 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2794 3414 4091 5239 5484 1606
Size: 1188 620 677 1148 245 2120
Fragments arranged by size:
2120 1188 1148 677 620 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5446
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5998
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5998
NotI GC'GGCC_GC
Cuts at: 3445 3445
Size: 5998
NspI r_CATG'y
Cuts at: 1132 1229 2241 3356 4901 1132
Size: 97 1012 1115 1545 2229
Fragments arranged by size:
2229 1545 1115 1012 97
PasI CC'CwG_GG
Cuts at: 1348 3109 1348
Size: 1761 4237
PciI A'CATG_T
Cuts at: 4897 4897
Size: 5998
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2893 1108
Size: 1785 4213
PmeI GTTT'AAAC
Cuts at: 3302 3302
Size: 5998
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4067 4099 5580 5612 4067
Size: 32 1481 32 4453
Fragments arranged by size:
4453 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3168 3168
Size: 5998
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5998
PsiI TTA'TAA
Cuts at: 2598 3568 2598
Size: 970 5028
PstI C_TGCA'G
Cuts at: 830 3350 830
Size: 2520 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5998
PvuII CAG'CTG
Cuts at: 2794 2794
Size: 5998
SalI G'TCGA_C
Cuts at: 1936 3340 1936
Size: 1404 4594
SanDI GG'GwC_CC
Cuts at: 3168 3168
Size: 5998
SbfI CC_TGCA'GG
Cuts at: 3350 3350
Size: 5998
ScaI AGT'ACT
Cuts at: 4246 5958 4246
Size: 1712 4286
SfcI C'TryA_G
Cuts at: 826 1045 2856 3346 4481 5162 5353 826
Size: 219 1811 490 1135 681 191 1471
Fragments arranged by size:
1811 1471 1135 681 490 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5998
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5998
SmaI CCC'GGG
Cuts at: 1872 3325 1872
Size: 1453 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2181 3256 4102 5003 5265 820
Size: 197 926 238 1075 846 901 262 1553
Fragments arranged by size:
1553 1075 926 901 846 262 238 197
SnaBI TAC'GTA
Cuts at: 493 3789 493
Size: 3296 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5998
SphI G_CATG'C
Cuts at: 1132 3356 1132
Size: 2224 3774
SspI AAT'ATT
Cuts at: 5 52 3922 5
Size: 47 3870 2081
Fragments arranged by size:
3870 2081 47
StuI AGG'CCT
Cuts at: 1517 3204 1517
Size: 1687 4311
StyI C'CwwG_G
Cuts at: 513 1065 3437 513
Size: 552 2372 3074
Fragments arranged by size:
3074 2372 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4025 4042 4195 4380 4825 325
Size: 1255 2445 17 153 185 445 1498
Fragments arranged by size:
2445 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4244 5956 96
Size: 274 80 33 51 1443 2267 1712 138
Fragments arranged by size:
2267 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2791 2823 5958 5990 2791
Size: 32 3135 32 2799
Fragments arranged by size:
3135 2799 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5998
XbaI T'CTAG_A
Cuts at: 2104 3334 2104
Size: 1230 4768
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3150 3150
Size: 5998
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5998
XmaI C'CCGG_G
Cuts at: 1870 3323 1870
Size: 1453 4545
XmnI GAAnn'nnTTC
Cuts at: 4127 4127
Size: 5998
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3806 276
Size: 53 83 186 936 2272 2468
Fragments arranged by size:
2468 2272 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I
EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI
SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I DraIII EcoICRI EcoNI FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PspOMI PspXI PsrI RsrII SacI SacII SapI SexAI
SfiI SfoI SrfI SwaI