(Circular) (Six-base) MAPSORT of: pFN21AB0968.seq Check: 7994 from: 1 to: 5998 pFN21AB0968 5998 bp With 182 enzymes: SgfI * April 15, 2010 09:33 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2242 3077 2242 Size: 835 5163 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3808 278 Size: 53 83 186 936 2272 2468 Fragments arranged by size: 2468 2272 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2379 3341 1937 Size: 442 962 4594 Fragments arranged by size: 4594 962 442 Acc65I G'GTAC_C Cuts at: 3319 3319 Size: 5998 AclI AA'CG_TT Cuts at: 1563 4125 4498 1563 Size: 2562 373 3063 Fragments arranged by size: 3063 2562 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2957 4061 5445 5713 1632 Size: 1325 1104 1384 268 1917 Fragments arranged by size: 1917 1384 1325 1104 268 AfeI AGC'GCT Cuts at: 3089 3089 Size: 5998 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5801 AflIII A'CryG_T Cuts at: 1203 4897 5874 1203 Size: 3694 977 1327 Fragments arranged by size: 3694 1327 977 AgeI A'CCGG_T Cuts at: 4829 4829 Size: 5998 AhdI GACnn_n'nnGTC Cuts at: 4727 4727 Size: 5998 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5998 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5998 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3047 3079 3047 Size: 32 5966 AlwNI CAG_nnn'CTG Cuts at: 2861 3741 5313 2861 Size: 880 1572 3546 Fragments arranged by size: 3546 1572 880 ApaI G_GGCC'C Cuts at: 2310 2664 2310 Size: 354 5644 ApaLI G'TGCA_C Cuts at: 4055 5211 4055 Size: 1156 4842 ApoI r'AATT_y Cuts at: 1493 2081 3307 3533 3685 5794 1493 Size: 588 1226 226 152 2109 1697 Fragments arranged by size: 2109 1697 1226 588 226 152 AseI AT'TA_AT Cuts at: 160 1032 4552 160 Size: 872 3520 1606 Fragments arranged by size: 3520 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5998 AvaI C'yCGr_G Cuts at: 1870 1943 3051 3145 3323 1870 Size: 73 1108 94 178 4545 Fragments arranged by size: 4545 1108 178 94 73 BamHI G'GATC_C Cuts at: 3328 3695 3328 Size: 367 5631 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2315 3319 4774 618 Size: 325 217 627 528 1004 1455 1842 Fragments arranged by size: 1842 1455 1004 627 528 325 217 BanII G_rGCy'C Cuts at: 1391 2226 2310 2664 3317 1391 Size: 835 84 354 653 4072 Fragments arranged by size: 4072 835 653 354 84 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5027 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4189 4223 1903 Size: 34 2252 34 3678 Fragments arranged by size: 3678 2252 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3890 5100 3890 Size: 1210 4788 BclI T'GATC_A Cuts at: 1548 1548 Size: 5998 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2347 4609 136 Size: 107 122 71 631 605 675 2262 1525 Fragments arranged by size: 2262 1525 675 631 605 122 107 71 BglII A'GATC_T Cuts at: 5993 5993 Size: 5998 BlpI GC'TnA_GC Cuts at: 2565 3415 2565 Size: 850 5148 Bme1580I G_kGCm'C Cuts at: 1790 2310 2343 2352 2664 4059 5215 1790 Size: 520 33 9 312 1395 1156 2573 Fragments arranged by size: 2573 1395 1156 520 312 33 9 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5998 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2367 2775 3153 Size: 416 53 83 399 24 950 408 378 Cuts at: 3153 3729 4687 442 Size: 576 958 1753 Fragments arranged by size: 1753 958 950 576 416 408 399 378 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5998 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3027 3027 Size: 5998 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2029 2232 2514 3047 3282 3311 4658 1239 Size: 790 203 282 533 235 29 1347 2579 Fragments arranged by size: 2579 1347 790 533 282 235 203 29 Bpu10I CC'TnA_GC Cuts at: 3110 3273 3110 Size: 163 5835 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2042 4123 4988 5286 2042 Size: 2081 865 298 2754 Fragments arranged by size: 2754 2081 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4661 882 Size: 617 3162 2219 Fragments arranged by size: 3162 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3789 493 Size: 711 2585 2702 Fragments arranged by size: 2702 2585 711 BsaBI GATnn'nnATC Cuts at: 2193 5992 2193 Size: 3799 2199 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2009 3218 3805 4187 Size: 53 83 186 936 476 1209 587 382 Cuts at: 4187 4822 275 Size: 635 1451 Fragments arranged by size: 1451 1209 936 635 587 476 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4431 4829 5103 5250 5661 4431 Size: 398 274 147 411 4768 Fragments arranged by size: 4768 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2027 2057 2455 2485 3047 Size: 30 165 30 624 30 398 30 562 Cuts at: 3047 3077 1178 Size: 30 4099 Fragments arranged by size: 4099 624 562 398 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2039 2797 3218 1186 Size: 853 758 421 3966 Fragments arranged by size: 3966 853 758 421 BseYI C'CCAG_C Cuts at: 2583 2635 2664 2845 2861 3175 5201 5697 2583 Size: 52 29 181 16 314 2026 496 2884 Fragments arranged by size: 2884 2026 496 314 181 52 29 16 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2095 1163 Size: 573 359 5066 Fragments arranged by size: 5066 573 359 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3448 4209 5237 1540 Size: 275 186 1447 761 1028 2301 Fragments arranged by size: 2301 1447 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4059 4144 5215 4059 Size: 85 1071 4842 Fragments arranged by size: 4842 1071 85 BsmI GAATG_Cn' Cuts at: 3509 3602 3509 Size: 93 5905 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2226 2310 2343 2352 2664 3317 4059 Size: 399 436 84 33 9 312 653 742 Cuts at: 4059 4144 5215 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 653 436 399 312 85 84 33 9 BspEI T'CCGG_A Cuts at: 5661 5661 Size: 5998 BspHI T'CATG_A Cuts at: 3887 5617 3887 Size: 1730 4268 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2242 3077 3353 844 Size: 1398 835 276 3489 Fragments arranged by size: 3489 1398 835 276 BsrBI CCG'CTC Cuts at: 1394 1823 2842 3885 1394 Size: 429 1019 1043 3507 Fragments arranged by size: 3507 1043 1019 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4493 4667 65 Size: 1183 3245 174 1396 Fragments arranged by size: 3245 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4642 4829 1700 Size: 110 2832 187 2869 Fragments arranged by size: 2869 2832 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5998 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5998 BssSI C'ACGA_G Cuts at: 4058 5070 4058 Size: 1012 4986 BstAPI GCAn_nnn'nTGC Cuts at: 794 2674 794 Size: 1880 4118 BstBI TT'CG_AA Cuts at: 4802 4802 Size: 5998 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5998 BstXI CCAn_nnnn'nTGG Cuts at: 2053 2182 2327 2531 2704 2900 3175 2053 Size: 129 145 204 173 196 275 4876 Fragments arranged by size: 4876 275 204 196 173 145 129 BstYI r'GATC_y Cuts at: 1972 3328 3695 4080 4097 5549 5635 5647 5993 Size: 1356 367 385 17 1452 86 12 346 Cuts at: 5993 1972 Size: 1977 Fragments arranged by size: 1977 1452 1356 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2260 2260 Size: 5998 BtgI C'CryG_G Cuts at: 513 1065 2004 2181 2474 2750 3130 513 Size: 552 939 177 293 276 380 3381 Fragments arranged by size: 3381 939 552 380 293 276 177 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5259 Fragments arranged by size: 5259 683 56 BtsI GCAGTG_nn' Cuts at: 2164 2546 2709 2797 3514 4308 4328 2164 Size: 382 163 88 717 794 20 3834 Fragments arranged by size: 3834 794 717 382 163 88 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5998 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2068 2103 529 Size: 35 1504 35 4424 Fragments arranged by size: 4424 1504 35 35 DraI TTT'AAA Cuts at: 3302 3302 Size: 5998 DraIII CAC_nnn'GTG Cuts at: 2756 2756 Size: 5998 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5005 809 Size: 507 3689 1802 Fragments arranged by size: 3689 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2055 3445 4334 Size: 54 1425 50 275 18 225 1390 889 Cuts at: 4334 5963 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1390 889 275 225 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3445 1537 Size: 275 1633 4090 Fragments arranged by size: 4090 1633 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2362 2996 3195 3928 1353 Size: 1009 634 199 733 3423 Fragments arranged by size: 3423 1009 733 634 199 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4585 4959 5105 387 Size: 1064 138 2996 374 146 1280 Fragments arranged by size: 2996 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2029 2232 2514 2957 3047 3282 3311 Size: 393 397 203 282 443 90 235 29 Cuts at: 3311 4061 4658 5445 5713 1239 Size: 750 597 787 268 1524 Fragments arranged by size: 1524 787 750 597 443 397 393 282 268 235 203 90 29 EcoNI CCTnn'n_nnAGG Cuts at: 2021 2021 Size: 5998 EcoO109I rG'GnC_Cy Cuts at: 2323 2660 2661 2970 3149 2323 Size: 337 1 309 179 5172 Fragments arranged by size: 5172 337 309 179 1 EcoRI G'AATT_C Cuts at: 3307 3685 3307 Size: 378 5620 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2962 2962 Size: 5998 FspI TGC'GCA Cuts at: 3041 4504 3041 Size: 1463 4535 FspAI rTGC'GCAy Cuts at: 3041 3041 Size: 5998 HaeII r_GCGC'y Cuts at: 1678 2806 3091 5145 1678 Size: 1128 285 2054 2531 Fragments arranged by size: 2531 2054 1128 285 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4639 4671 4713 4745 1373 Size: 32 135 32 3067 32 42 32 2626 Fragments arranged by size: 3067 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3342 3588 669 Size: 988 281 1404 246 3079 Fragments arranged by size: 3079 1404 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5998 HpaI GTT'AAC Cuts at: 3588 3588 Size: 5998 KpnI G_GTAC'C Cuts at: 3323 3323 Size: 5998 MfeI C'AATT_G Cuts at: 3597 3597 Size: 5998 MluI A'CGCG_T Cuts at: 5874 5874 Size: 5998 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5112 5296 1305 Size: 3807 184 2007 Fragments arranged by size: 3807 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2057 10 Size: 54 1425 343 225 3951 Fragments arranged by size: 3951 1425 343 225 54 MslI CAynn'nnrTG Cuts at: 518 1166 2406 3956 4315 4474 518 Size: 648 1240 1550 359 159 2042 Fragments arranged by size: 2042 1550 1240 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2587 2858 2889 3414 4091 5239 5484 1606 Size: 981 271 31 525 677 1148 245 2120 Fragments arranged by size: 2120 1148 981 677 525 271 245 31 NcoI C'CATG_G Cuts at: 513 1065 2004 2181 2474 513 Size: 552 939 177 293 4037 Fragments arranged by size: 4037 939 552 293 177 NdeI CA'TA_TG Cuts at: 387 387 Size: 5998 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5998 NotI GC'GGCC_GC Cuts at: 3445 3445 Size: 5998 NspI r_CATG'y Cuts at: 1132 1229 2672 3356 4901 1132 Size: 97 1443 684 1545 2229 Fragments arranged by size: 2229 1545 1443 684 97 PasI CC'CwG_GG Cuts at: 1348 2303 2327 1348 Size: 955 24 5019 Fragments arranged by size: 5019 955 24 PciI A'CATG_T Cuts at: 4897 4897 Size: 5998 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5998 PmeI GTTT'AAAC Cuts at: 3302 3302 Size: 5998 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3047 3079 4067 4099 5580 5612 3047 Size: 32 988 32 1481 32 3433 Fragments arranged by size: 3433 1481 988 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2323 3149 2323 Size: 826 5172 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5998 PsiI TTA'TAA Cuts at: 3568 3568 Size: 5998 PspOMI G'GGCC_C Cuts at: 2306 2660 2306 Size: 354 5644 PstI C_TGCA'G Cuts at: 830 2239 3350 830 Size: 1409 1111 3478 Fragments arranged by size: 3478 1409 1111 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5998 PvuII CAG'CTG Cuts at: 2587 2889 2587 Size: 302 5696 SalI G'TCGA_C Cuts at: 1936 3340 1936 Size: 1404 4594 SanDI GG'GwC_CC Cuts at: 3149 3149 Size: 5998 SapI GCTCTTCn'nnn_ Cuts at: 2362 2996 2362 Size: 634 5364 SbfI CC_TGCA'GG Cuts at: 2239 3350 2239 Size: 1111 4887 ScaI AGT'ACT Cuts at: 4246 5958 4246 Size: 1712 4286 SfcI C'TryA_G Cuts at: 826 1045 2235 3346 4481 5162 5353 826 Size: 219 1190 1111 1135 681 191 1471 Fragments arranged by size: 1471 1190 1135 1111 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5998 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5998 SmaI CCC'GGG Cuts at: 1872 3053 3147 3325 1872 Size: 1181 94 178 4545 Fragments arranged by size: 4545 1181 178 94 SmlI C'TyrA_G Cuts at: 820 1017 1943 2021 4102 5003 5265 820 Size: 197 926 78 2081 901 262 1553 Fragments arranged by size: 2081 1553 926 901 262 197 78 SnaBI TAC'GTA Cuts at: 493 3789 493 Size: 3296 2702 SpeI A'CTAG_T Cuts at: 152 2335 152 Size: 2183 3815 SphI G_CATG'C Cuts at: 1132 3356 1132 Size: 2224 3774 SspI AAT'ATT Cuts at: 5 52 3922 5 Size: 47 3870 2081 Fragments arranged by size: 3870 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5998 StyI C'CwwG_G Cuts at: 513 1065 2004 2181 2343 2474 2697 3168 3437 Size: 552 939 177 162 131 223 471 269 Cuts at: 3437 513 Size: 3074 Fragments arranged by size: 3074 939 552 471 269 223 177 162 131 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2269 2333 3143 4025 4042 4195 4380 Size: 1255 689 64 810 882 17 153 185 Cuts at: 4380 4825 325 Size: 445 1498 Fragments arranged by size: 1498 1255 882 810 689 445 185 153 64 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4244 5956 96 Size: 274 80 33 51 1443 2267 1712 138 Fragments arranged by size: 2267 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2027 2059 2455 2487 5958 5990 2027 Size: 32 396 32 3471 32 2035 Fragments arranged by size: 3471 2035 396 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5998 XbaI T'CTAG_A Cuts at: 3334 3334 Size: 5998 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2300 2300 Size: 5998 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5998 XmaI C'CCGG_G Cuts at: 1870 3051 3145 3323 1870 Size: 1181 94 178 4545 Fragments arranged by size: 4545 1181 178 94 XmnI GAAnn'nnTTC Cuts at: 3062 4127 3062 Size: 1065 4933 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3806 276 Size: 53 83 186 936 2272 2468 Fragments arranged by size: 2468 2272 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstZ17I EcoICRI EcoRV FseI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacI SacII SexAI SfiI SfoI SrfI SwaI