(Circular) (Six-base) MAPSORT of: pFN21AB6781.seq Check: 4002 from: 1 to: 5065 pFN21AB6781 5065 bp With 182 enzymes: SgfI * April 15, 2010 09:33 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2120 2196 2120 Size: 76 4989 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2875 278 Size: 53 83 186 936 1339 2468 Fragments arranged by size: 2468 1339 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2408 1937 Size: 471 4594 Acc65I G'GTAC_C Cuts at: 2386 2386 Size: 5065 AclI AA'CG_TT Cuts at: 1563 3192 3565 1563 Size: 1629 373 3063 Fragments arranged by size: 3063 1629 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2141 3128 4512 4780 1632 Size: 509 987 1384 268 1917 Fragments arranged by size: 1917 1384 987 509 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 4868 AflIII A'CryG_T Cuts at: 1203 3964 4941 1203 Size: 2761 977 1327 Fragments arranged by size: 2761 1327 977 AgeI A'CCGG_T Cuts at: 3896 3896 Size: 5065 AhdI GACnn_n'nnGTC Cuts at: 3794 3794 Size: 5065 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5065 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5065 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2090 2122 2090 Size: 32 5033 AlwNI CAG_nnn'CTG Cuts at: 2808 4380 2808 Size: 1572 3493 ApaLI G'TGCA_C Cuts at: 3122 4278 3122 Size: 1156 3909 ApoI r'AATT_y Cuts at: 1493 2374 2600 2752 4861 1493 Size: 881 226 152 2109 1697 Fragments arranged by size: 2109 1697 881 226 152 AseI AT'TA_AT Cuts at: 160 1032 3619 160 Size: 872 2587 1606 Fragments arranged by size: 2587 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5065 AvaI C'yCGr_G Cuts at: 1870 1943 2390 1870 Size: 73 447 4545 Fragments arranged by size: 4545 447 73 BamHI G'GATC_C Cuts at: 2272 2395 2762 2272 Size: 123 367 4575 Fragments arranged by size: 4575 367 123 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2386 3841 618 Size: 325 217 627 599 1455 1842 Fragments arranged by size: 1842 1455 627 599 325 217 BanII G_rGCy'C Cuts at: 1391 2384 1391 Size: 993 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4094 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2211 2245 3256 3290 1903 Size: 34 274 34 1011 34 3678 Fragments arranged by size: 3678 1011 274 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2156 2957 4167 2156 Size: 801 1210 3054 Fragments arranged by size: 3054 1210 801 BclI T'GATC_A Cuts at: 1548 1548 Size: 5065 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3676 136 Size: 107 122 71 631 605 2004 1525 Fragments arranged by size: 2004 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5060 5060 Size: 5065 BlpI GC'TnA_GC Cuts at: 2482 2482 Size: 5065 Bme1580I G_kGCm'C Cuts at: 1790 3126 4282 1790 Size: 1336 1156 2573 Fragments arranged by size: 2573 1336 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5065 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2260 2796 3754 Size: 416 53 83 399 24 843 536 958 Cuts at: 3754 442 Size: 1753 Fragments arranged by size: 1753 958 843 536 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5065 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3725 1239 Size: 2486 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3190 4055 4353 3190 Size: 865 298 3902 Fragments arranged by size: 3902 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2017 3728 882 Size: 617 518 1711 2219 Fragments arranged by size: 2219 1711 617 518 BsaAI yAC'GTr Cuts at: 493 1204 2856 493 Size: 711 1652 2702 Fragments arranged by size: 2702 1652 711 BsaBI GATnn'nnATC Cuts at: 5059 5059 Size: 5065 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2872 3254 3889 275 Size: 53 83 186 936 1339 382 635 1451 Fragments arranged by size: 1451 1339 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3498 3896 4170 4317 4728 3498 Size: 398 274 147 411 3835 Fragments arranged by size: 3835 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2334 2364 1178 Size: 30 165 30 931 30 3879 Fragments arranged by size: 3879 931 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2342 1186 Size: 1156 3909 BseYI C'CCAG_C Cuts at: 2346 4268 4764 2346 Size: 1922 496 2647 Fragments arranged by size: 2647 1922 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2215 1163 Size: 573 479 4013 Fragments arranged by size: 4013 573 479 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2515 3276 4304 1540 Size: 275 186 514 761 1028 2301 Fragments arranged by size: 2301 1028 761 514 275 186 BsiHKAI G_wGCw'C Cuts at: 3126 3211 4282 3126 Size: 85 1071 3909 Fragments arranged by size: 3909 1071 85 BsmI GAATG_Cn' Cuts at: 2576 2669 2576 Size: 93 4972 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2384 3126 3211 4282 1391 Size: 399 594 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 594 399 85 BspEI T'CCGG_A Cuts at: 4728 4728 Size: 5065 BspHI T'CATG_A Cuts at: 2954 4684 2954 Size: 1730 3335 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2120 2196 2420 844 Size: 1276 76 224 3489 Fragments arranged by size: 3489 1276 224 76 BsrBI CCG'CTC Cuts at: 1394 1823 2952 1394 Size: 429 1129 3507 Fragments arranged by size: 3507 1129 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3560 3734 65 Size: 1183 2312 174 1396 Fragments arranged by size: 2312 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3709 3896 1700 Size: 110 1899 187 2869 Fragments arranged by size: 2869 1899 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5065 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5065 BssSI C'ACGA_G Cuts at: 3125 4137 3125 Size: 1012 4053 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5065 BstBI TT'CG_AA Cuts at: 3869 3869 Size: 5065 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5065 BstYI r'GATC_y Cuts at: 1972 2272 2395 2762 3147 3164 4616 4702 4714 Size: 300 123 367 385 17 1452 86 12 Cuts at: 4714 5060 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 385 367 346 300 123 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2310 513 Size: 552 1245 3268 Fragments arranged by size: 3268 1245 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4326 Fragments arranged by size: 4326 683 56 BtsI GCAGTG_nn' Cuts at: 2581 3375 3395 2581 Size: 794 20 4251 Fragments arranged by size: 4251 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5065 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5030 DraI TTT'AAA Cuts at: 2369 2369 Size: 5065 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2252 4072 809 Size: 507 936 1820 1802 Fragments arranged by size: 1820 1802 936 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2512 3401 5030 Size: 54 1425 50 275 18 682 889 1629 Cuts at: 5030 8 Size: 43 Fragments arranged by size: 1629 1425 889 682 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2512 1537 Size: 275 700 4090 Fragments arranged by size: 4090 700 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2995 1353 Size: 1642 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3652 4026 4172 387 Size: 1064 138 2063 374 146 1280 Fragments arranged by size: 2063 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2141 3128 3725 4512 4780 1239 Size: 393 509 987 597 787 268 1524 Fragments arranged by size: 1524 987 787 597 509 393 268 EcoO109I rG'GnC_Cy Cuts at: 2173 2173 Size: 5065 EcoRI G'AATT_C Cuts at: 2374 2752 2374 Size: 378 4687 FspI TGC'GCA Cuts at: 3571 3571 Size: 5065 HaeII r_GCGC'y Cuts at: 1678 4212 1678 Size: 2534 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3706 3738 3780 3812 1373 Size: 32 135 32 2134 32 42 32 2626 Fragments arranged by size: 2626 2134 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2342 2409 2655 669 Size: 988 281 404 67 246 3079 Fragments arranged by size: 3079 988 404 281 246 67 HindIII A'AGCT_T Cuts at: 748 748 Size: 5065 HpaI GTT'AAC Cuts at: 2655 2655 Size: 5065 KpnI G_GTAC'C Cuts at: 2390 2390 Size: 5065 MfeI C'AATT_G Cuts at: 2664 2664 Size: 5065 MluI A'CGCG_T Cuts at: 4941 4941 Size: 5065 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4179 4363 1305 Size: 2874 184 2007 Fragments arranged by size: 2874 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3243 Fragments arranged by size: 3243 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3023 3382 3541 518 Size: 648 1857 359 159 2042 Fragments arranged by size: 2042 1857 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2481 3158 4306 4551 1606 Size: 875 677 1148 245 2120 Fragments arranged by size: 2120 1148 875 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4513 NdeI CA'TA_TG Cuts at: 387 387 Size: 5065 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5065 NotI GC'GGCC_GC Cuts at: 2512 2512 Size: 5065 NspI r_CATG'y Cuts at: 1132 1229 2423 3968 1132 Size: 97 1194 1545 2229 Fragments arranged by size: 2229 1545 1194 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5065 PciI A'CATG_T Cuts at: 3964 3964 Size: 5065 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5065 PmeI GTTT'AAAC Cuts at: 2369 2369 Size: 5065 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3134 3166 4647 4679 3134 Size: 32 1481 32 3520 Fragments arranged by size: 3520 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5065 PsiI TTA'TAA Cuts at: 2635 2635 Size: 5065 PstI C_TGCA'G Cuts at: 830 2417 830 Size: 1587 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5065 SalI G'TCGA_C Cuts at: 1936 2407 1936 Size: 471 4594 SbfI CC_TGCA'GG Cuts at: 2417 2417 Size: 5065 ScaI AGT'ACT Cuts at: 3313 5025 3313 Size: 1712 3353 SfcI C'TryA_G Cuts at: 826 1045 2413 3548 4229 4420 826 Size: 219 1368 1135 681 191 1471 Fragments arranged by size: 1471 1368 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5065 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5065 SmaI CCC'GGG Cuts at: 1872 2392 1872 Size: 520 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3169 4070 4332 820 Size: 197 926 1226 901 262 1553 Fragments arranged by size: 1553 1226 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2856 493 Size: 2363 2702 SpeI A'CTAG_T Cuts at: 152 2152 152 Size: 2000 3065 SphI G_CATG'C Cuts at: 1132 2423 1132 Size: 1291 3774 SspI AAT'ATT Cuts at: 5 52 2989 5 Size: 47 2937 2081 Fragments arranged by size: 2937 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5065 StyI C'CwwG_G Cuts at: 513 1065 2144 2504 513 Size: 552 1079 360 3074 Fragments arranged by size: 3074 1079 552 360 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2083 3092 3109 3262 3447 3892 325 Size: 1255 503 1009 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1009 503 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2262 3311 5023 Size: 274 80 33 51 1443 285 1049 1712 Cuts at: 5023 96 Size: 138 Fragments arranged by size: 1712 1443 1049 285 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5025 5057 5025 Size: 32 5033 Tth111I GACn'n_nGTC Cuts at: 1316 2252 1316 Size: 936 4129 XbaI T'CTAG_A Cuts at: 2401 2401 Size: 5065 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5065 XmaI C'CCGG_G Cuts at: 1870 2390 1870 Size: 520 4545 XmnI GAAnn'nnTTC Cuts at: 3194 3194 Size: 5065 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2873 276 Size: 53 83 186 936 1339 2468 Fragments arranged by size: 2468 1339 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI