(Circular) (Six-base) MAPSORT of: pFN21AB6633.seq Check: 4312 from: 1 to: 5956 pFN21AB6633 5956 bp With 182 enzymes: SgfI * April 15, 2010 09:33 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2152 2152 Size: 5956 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3766 278 Size: 53 83 186 936 2230 2468 Fragments arranged by size: 2468 2230 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3299 1937 Size: 1362 4594 Acc65I G'GTAC_C Cuts at: 2598 3277 2598 Size: 679 5277 AclI AA'CG_TT Cuts at: 1563 4083 4456 1563 Size: 2520 373 3063 Fragments arranged by size: 3063 2520 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2973 4019 5403 5671 1632 Size: 1341 1046 1384 268 1917 Fragments arranged by size: 1917 1384 1341 1046 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5759 AflIII A'CryG_T Cuts at: 1203 4855 5832 1203 Size: 3652 977 1327 Fragments arranged by size: 3652 1327 977 AgeI A'CCGG_T Cuts at: 4787 4787 Size: 5956 AhdI GACnn_n'nnGTC Cuts at: 4685 4685 Size: 5956 AleI CACnn'nnGTG Cuts at: 1166 2684 1166 Size: 1518 4438 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5956 AlwNI CAG_nnn'CTG Cuts at: 3699 5271 3699 Size: 1572 4384 ApaLI G'TGCA_C Cuts at: 2060 4013 5169 2060 Size: 1953 1156 2847 Fragments arranged by size: 2847 1953 1156 ApoI r'AATT_y Cuts at: 1493 3265 3491 3643 5752 1493 Size: 1772 226 152 2109 1697 Fragments arranged by size: 2109 1772 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4510 160 Size: 872 3478 1606 Fragments arranged by size: 3478 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5956 AvaI C'yCGr_G Cuts at: 1870 1943 2033 2218 3281 1870 Size: 73 90 185 1063 4545 Fragments arranged by size: 4545 1063 185 90 73 BamHI G'GATC_C Cuts at: 3286 3653 3286 Size: 367 5589 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2123 2130 2222 2417 2598 Size: 325 217 627 336 7 92 195 181 Cuts at: 2598 3277 4732 618 Size: 679 1455 1842 Fragments arranged by size: 1842 1455 679 627 336 325 217 195 181 92 7 BanII G_rGCy'C Cuts at: 1391 2189 2407 3275 1391 Size: 798 218 868 4072 Fragments arranged by size: 4072 868 798 218 BbeI G_GCGC'C Cuts at: 2127 2134 2226 2421 2127 Size: 7 92 195 5662 Fragments arranged by size: 5662 195 92 7 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2988 928 Size: 971 1089 3896 Fragments arranged by size: 3896 1089 971 BbvCI CC'TCA_GC Cuts at: 2202 2202 Size: 5956 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4147 4181 1903 Size: 34 50 34 2126 34 3678 Fragments arranged by size: 3678 2126 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2603 3848 5058 2603 Size: 1245 1210 3501 Fragments arranged by size: 3501 1245 1210 BclI T'GATC_A Cuts at: 1548 2776 1548 Size: 1228 4728 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4567 136 Size: 107 122 71 631 605 2895 1525 Fragments arranged by size: 2895 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5951 5951 Size: 5956 BlpI GC'TnA_GC Cuts at: 2177 3373 2177 Size: 1196 4760 Bme1580I G_kGCm'C Cuts at: 1790 2064 4017 5173 1790 Size: 274 1953 1156 2573 Fragments arranged by size: 2573 1953 1156 274 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5956 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3687 4645 442 Size: 416 53 83 399 24 2270 958 1753 Fragments arranged by size: 2270 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5956 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2355 4616 1239 Size: 1116 2261 2579 Fragments arranged by size: 2579 2261 1116 Bpu10I CC'TnA_GC Cuts at: 2202 2202 Size: 5956 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4081 4946 5244 4081 Size: 865 298 4793 Fragments arranged by size: 4793 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2642 4619 882 Size: 617 1143 1977 2219 Fragments arranged by size: 2219 1977 1143 617 BsaAI yAC'GTr Cuts at: 493 1204 2682 2686 3747 493 Size: 711 1478 4 1061 2702 Fragments arranged by size: 2702 1478 1061 711 4 BsaBI GATnn'nnATC Cuts at: 2775 5950 2775 Size: 3175 2781 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2124 2131 2223 2418 Size: 53 83 186 936 591 7 92 195 Cuts at: 2418 2675 3763 4145 4780 275 Size: 257 1088 382 635 1451 Fragments arranged by size: 1451 1088 936 635 591 382 257 195 186 92 83 53 7 BsaWI w'CCGG_w Cuts at: 4389 4787 5061 5208 5619 4389 Size: 398 274 147 411 4726 Fragments arranged by size: 4726 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5731 Fragments arranged by size: 5731 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2956 1186 Size: 1770 4186 BseYI C'CCAG_C Cuts at: 2256 2483 2840 3149 5159 5655 2256 Size: 227 357 309 2010 496 2557 Fragments arranged by size: 2557 2010 496 357 309 227 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2259 2708 3012 3093 3223 1163 Size: 573 523 449 304 81 130 3896 Fragments arranged by size: 3896 573 523 449 304 130 81 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2325 2480 3406 4167 5195 1540 Size: 275 186 324 155 926 761 1028 2301 Fragments arranged by size: 2301 1028 926 761 324 275 186 155 BsiHKAI G_wGCw'C Cuts at: 2028 2064 4017 4102 5173 2028 Size: 36 1953 85 1071 2811 Fragments arranged by size: 2811 1953 1071 85 36 BsiWI C'GTAC_G Cuts at: 2682 2682 Size: 5956 BsmI GAATG_Cn' Cuts at: 3467 3560 3467 Size: 93 5863 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2028 2064 2189 2407 3275 4017 4102 Size: 399 238 36 125 218 868 742 85 Cuts at: 4102 5173 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 868 742 399 238 218 125 85 36 BspEI T'CCGG_A Cuts at: 5619 5619 Size: 5956 BspHI T'CATG_A Cuts at: 3845 5575 3845 Size: 1730 4226 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2152 2481 2586 2928 3311 844 Size: 1308 329 105 342 383 3489 Fragments arranged by size: 3489 1308 383 342 329 105 BsrBI CCG'CTC Cuts at: 1394 1823 3843 1394 Size: 429 2020 3507 Fragments arranged by size: 3507 2020 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4451 4625 65 Size: 1183 3203 174 1396 Fragments arranged by size: 3203 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2081 2110 2324 2601 4600 4787 1700 Size: 110 271 29 214 277 1999 187 2869 Fragments arranged by size: 2869 1999 277 271 214 187 110 29 BsrGI T'GTAC_A Cuts at: 96 2703 96 Size: 2607 3349 BssHII G'CGCG_C Cuts at: 1925 2052 2425 1925 Size: 127 373 5456 Fragments arranged by size: 5456 373 127 BssSI C'ACGA_G Cuts at: 4016 5028 4016 Size: 1012 4944 BstAPI GCAn_nnn'nTGC Cuts at: 794 3026 794 Size: 2232 3724 BstBI TT'CG_AA Cuts at: 4760 4760 Size: 5956 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5956 BstXI CCAn_nnnn'nTGG Cuts at: 2491 3157 2491 Size: 666 5290 BstYI r'GATC_y Cuts at: 1972 2139 3286 3653 4038 4055 5507 5593 5605 Size: 167 1147 367 385 17 1452 86 12 Cuts at: 5605 5951 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1147 385 367 346 167 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3232 3232 Size: 5956 BtgI C'CryG_G Cuts at: 513 1065 2304 513 Size: 552 1239 4165 Fragments arranged by size: 4165 1239 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5217 Fragments arranged by size: 5217 683 56 BtsI GCAGTG_nn' Cuts at: 2062 3472 4266 4286 2062 Size: 1410 794 20 3732 Fragments arranged by size: 3732 1410 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5956 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5921 DraI TTT'AAA Cuts at: 3260 3260 Size: 5956 DraIII CAC_nnn'GTG Cuts at: 2785 2785 Size: 5956 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4963 809 Size: 507 3647 1802 Fragments arranged by size: 3647 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2083 2322 2347 Size: 54 1425 50 275 18 253 239 25 Cuts at: 2347 2477 2652 2739 3403 4292 5921 8 Size: 130 175 87 664 889 1629 43 Fragments arranged by size: 1629 1425 889 664 275 253 239 175 130 87 54 50 43 25 18 EagI C'GGCC_G Cuts at: 1537 1812 2322 2477 3403 1537 Size: 275 510 155 926 4090 Fragments arranged by size: 4090 926 510 275 155 EarI CTCTTCn'nnn_ Cuts at: 1353 3886 1353 Size: 2533 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2242 2738 4543 4917 5063 387 Size: 1064 138 653 496 1805 374 146 1280 Fragments arranged by size: 1805 1280 1064 653 496 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2355 2973 4019 4616 5403 5671 1239 Size: 393 723 618 1046 597 787 268 1524 Fragments arranged by size: 1524 1046 787 723 618 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2215 2215 Size: 5956 EcoRI G'AATT_C Cuts at: 3265 3643 3265 Size: 378 5578 FspI TGC'GCA Cuts at: 4462 4462 Size: 5956 HaeII r_GCGC'y Cuts at: 1678 2127 2134 2209 2226 2421 2447 5103 1678 Size: 449 7 75 17 195 26 2656 2531 Fragments arranged by size: 2656 2531 449 195 75 26 17 7 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4597 4629 4671 4703 1373 Size: 32 135 32 3025 32 42 32 2626 Fragments arranged by size: 3025 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3300 3546 669 Size: 988 281 1362 246 3079 Fragments arranged by size: 3079 1362 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5956 HpaI GTT'AAC Cuts at: 3546 3546 Size: 5956 KasI G'GCGC_C Cuts at: 2123 2130 2222 2417 2123 Size: 7 92 195 5662 Fragments arranged by size: 5662 195 92 7 KpnI G_GTAC'C Cuts at: 2602 3281 2602 Size: 679 5277 MfeI C'AATT_G Cuts at: 3555 3555 Size: 5956 MluI A'CGCG_T Cuts at: 5832 5832 Size: 5956 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5070 5254 1305 Size: 3765 184 2007 Fragments arranged by size: 3765 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2741 10 Size: 54 1425 343 909 3225 Fragments arranged by size: 3225 1425 909 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2684 3225 3914 4273 4432 518 Size: 648 1518 541 689 359 159 2042 Fragments arranged by size: 2042 1518 689 648 541 359 159 MspA1I CmG'CkG Cuts at: 1606 2306 2345 3372 4049 5197 5442 1606 Size: 700 39 1027 677 1148 245 2120 Fragments arranged by size: 2120 1148 1027 700 677 245 39 NaeI GCC'GGC Cuts at: 2083 2112 2326 2083 Size: 29 214 5713 Fragments arranged by size: 5713 214 29 NarI GG'CG_CC Cuts at: 2124 2131 2223 2418 2124 Size: 7 92 195 5662 Fragments arranged by size: 5662 195 92 7 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5404 NdeI CA'TA_TG Cuts at: 387 387 Size: 5956 NgoMIV G'CCGG_C Cuts at: 2081 2110 2324 2081 Size: 29 214 5713 Fragments arranged by size: 5713 214 29 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5956 NotI GC'GGCC_GC Cuts at: 2477 3403 2477 Size: 926 5030 NspI r_CATG'y Cuts at: 1132 1229 3024 3314 4859 1132 Size: 97 1795 290 1545 2229 Fragments arranged by size: 2229 1795 1545 290 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5956 PciI A'CATG_T Cuts at: 4855 4855 Size: 5956 PflMI CCAn_nnn'nTGG Cuts at: 1108 2896 1108 Size: 1788 4168 PmeI GTTT'AAAC Cuts at: 3260 3260 Size: 5956 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4025 4057 5538 5570 4025 Size: 32 1481 32 4411 Fragments arranged by size: 4411 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2215 2215 Size: 5956 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5956 PsiI TTA'TAA Cuts at: 3526 3526 Size: 5956 PstI C_TGCA'G Cuts at: 830 3308 830 Size: 2478 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5956 SacII CC_GC'GG Cuts at: 2307 2307 Size: 5956 SalI G'TCGA_C Cuts at: 1936 3298 1936 Size: 1362 4594 SanDI GG'GwC_CC Cuts at: 2215 2215 Size: 5956 SbfI CC_TGCA'GG Cuts at: 3308 3308 Size: 5956 ScaI AGT'ACT Cuts at: 2534 4204 5916 2534 Size: 1670 1712 2574 Fragments arranged by size: 2574 1712 1670 SexAI A'CCwGG_T Cuts at: 2646 3156 2646 Size: 510 5446 SfcI C'TryA_G Cuts at: 826 1045 3304 4439 5120 5311 826 Size: 219 2259 1135 681 191 1471 Fragments arranged by size: 2259 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2125 2132 2224 2419 2125 Size: 7 92 195 5662 Fragments arranged by size: 5662 195 92 7 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5956 SgrAI Cr'CCGG_yG Cuts at: 1700 2110 1700 Size: 410 5546 SmaI CCC'GGG Cuts at: 1872 2220 3283 1872 Size: 348 1063 4545 Fragments arranged by size: 4545 1063 348 SmlI C'TyrA_G Cuts at: 820 1017 1943 4060 4961 5223 820 Size: 197 926 2117 901 262 1553 Fragments arranged by size: 2117 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3747 493 Size: 3254 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5956 SphI G_CATG'C Cuts at: 1132 3314 1132 Size: 2182 3774 SspI AAT'ATT Cuts at: 5 52 3880 5 Size: 47 3828 2081 Fragments arranged by size: 3828 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5956 StyI C'CwwG_G Cuts at: 513 1065 2818 3395 513 Size: 552 1753 577 3074 Fragments arranged by size: 3074 1753 577 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2951 3983 4000 4153 4338 4783 325 Size: 1255 1371 1032 17 153 185 445 1498 Fragments arranged by size: 1498 1371 1255 1032 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2532 2703 2744 Size: 274 80 33 51 1443 555 171 41 Cuts at: 2744 3023 4202 5914 96 Size: 279 1179 1712 138 Fragments arranged by size: 1712 1443 1179 555 279 274 171 138 80 51 41 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5916 5948 5916 Size: 32 5924 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5956 XbaI T'CTAG_A Cuts at: 2399 3292 2399 Size: 893 5063 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2856 2856 Size: 5956 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5956 XmaI C'CCGG_G Cuts at: 1870 2218 3281 1870 Size: 348 1063 4545 Fragments arranged by size: 4545 1063 348 XmnI GAAnn'nnTTC Cuts at: 4085 4085 Size: 5956 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3764 276 Size: 53 83 186 936 2230 2468 Fragments arranged by size: 2468 2230 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacII SalI SanDI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BfrBI BplI BsmBI BstZ17I EcoICRI EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SapI SfiI SrfI SwaI