Restriction map for
pFN21AB6633
(Linear) (Six-base) MAPSORT of: KIBB6633.seq Check: 3510 from: 1 to: 1251
KIBB6633.orf
With 182 enzymes: SgfI *
October 7, 2009 14:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 147 1251
Size: 147 1104
Acc65I G'GTAC_C
Cuts at: 0 593 1251
Size: 593 658
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 968 1251
Size: 968 283
AleI CACnn'nnGTG
Cuts at: 0 679 1251
Size: 679 572
ApaLI G'TGCA_C
Cuts at: 0 55 1251
Size: 55 1196
AvaI C'yCGr_G
Cuts at: 0 28 213 1251
Size: 28 185 1038
Fragments arranged by size:
1038 185 28
BanI G'GyrC_C
Cuts at: 0 118 125 217 412 593 1251
Size: 118 7 92 195 181 658
Fragments arranged by size:
658 195 181 118 92 7
BanII G_rGCy'C
Cuts at: 0 184 402 1251
Size: 184 218 849
Fragments arranged by size:
849 218 184
BbeI G_GCGC'C
Cuts at: 0 122 129 221 416 1251
Size: 122 7 92 195 835
Fragments arranged by size:
835 195 122 92 7
BbsI GAAGACnn'nnnn_
Cuts at: 0 983 1251
Size: 983 268
BbvCI CC'TCA_GC
Cuts at: 0 197 1251
Size: 197 1054
BciVI GTATCCnnnnn_n'
Cuts at: 0 598 1251
Size: 598 653
BclI T'GATC_A
Cuts at: 0 771 1251
Size: 771 480
BlpI GC'TnA_GC
Cuts at: 0 172 1251
Size: 172 1079
Bme1580I G_kGCm'C
Cuts at: 0 59 1251
Size: 59 1192
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 350 1251
Size: 350 901
Bpu10I CC'TnA_GC
Cuts at: 0 197 1251
Size: 197 1054
BsaI GGTCTCn'nnnn_
Cuts at: 0 637 1251
Size: 637 614
BsaAI yAC'GTr
Cuts at: 0 677 681 1251
Size: 677 4 570
Fragments arranged by size:
677 570 4
BsaBI GATnn'nnATC
Cuts at: 0 770 1251
Size: 770 481
BsaHI Gr'CG_yC
Cuts at: 0 119 126 218 413 670 1251
Size: 119 7 92 195 257 581
Fragments arranged by size:
581 257 195 119 92 7
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 951 1251
Size: 951 300
BseYI C'CCAG_C
Cuts at: 0 251 478 835 1144 1251
Size: 251 227 357 309 107
Fragments arranged by size:
357 309 251 227 107
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 254 703 1007 1088 1218 1251
Size: 254 449 304 81 130 33
Fragments arranged by size:
449 304 254 130 81 33
BsiEI CG_ry'CG
Cuts at: 0 320 475 1251
Size: 320 155 776
Fragments arranged by size:
776 320 155
BsiHKAI G_wGCw'C
Cuts at: 0 23 59 1251
Size: 23 36 1192
Fragments arranged by size:
1192 36 23
BsiWI C'GTAC_G
Cuts at: 0 677 1251
Size: 677 574
Bsp1286I G_dGCh'C
Cuts at: 0 23 59 184 402 1251
Size: 23 36 125 218 849
Fragments arranged by size:
849 218 125 36 23
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 147 476 581 923 1251
Size: 147 329 105 342 328
Fragments arranged by size:
342 329 328 147 105
BsrFI r'CCGG_y
Cuts at: 0 76 105 319 596 1251
Size: 76 29 214 277 655
Fragments arranged by size:
655 277 214 76 29
BsrGI T'GTAC_A
Cuts at: 0 698 1251
Size: 698 553
BssHII G'CGCG_C
Cuts at: 0 47 420 1251
Size: 47 373 831
Fragments arranged by size:
831 373 47
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1021 1251
Size: 1021 230
BstXI CCAn_nnnn'nTGG
Cuts at: 0 486 1152 1251
Size: 486 666 99
Fragments arranged by size:
666 486 99
BstYI r'GATC_y
Cuts at: 0 134 1251
Size: 134 1117
Bsu36I CC'TnA_GG
Cuts at: 0 1227 1251
Size: 1227 24
BtgI C'CryG_G
Cuts at: 0 299 1251
Size: 299 952
BtsI GCAGTG_nn'
Cuts at: 0 57 1251
Size: 57 1194
DraIII CAC_nnn'GTG
Cuts at: 0 780 1251
Size: 780 471
EaeI y'GGCC_r
Cuts at: 0 78 317 342 472 647 734 1251
Size: 78 239 25 130 175 87 517
Fragments arranged by size:
517 239 175 130 87 78 25
EagI C'GGCC_G
Cuts at: 0 317 472 1251
Size: 317 155 779
Fragments arranged by size:
779 317 155
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 237 733 1251
Size: 237 496 518
Fragments arranged by size:
518 496 237
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 350 968 1251
Size: 350 618 283
Fragments arranged by size:
618 350 283
EcoO109I rG'GnC_Cy
Cuts at: 0 210 1251
Size: 210 1041
HaeII r_GCGC'y
Cuts at: 0 122 129 204 221 416 442 1251
Size: 122 7 75 17 195 26 809
Fragments arranged by size:
809 195 122 75 26 17 7
KasI G'GCGC_C
Cuts at: 0 118 125 217 412 1251
Size: 118 7 92 195 839
Fragments arranged by size:
839 195 118 92 7
KpnI G_GTAC'C
Cuts at: 0 597 1251
Size: 597 654
MscI TGG'CCA
Cuts at: 0 736 1251
Size: 736 515
MslI CAynn'nnrTG
Cuts at: 0 679 1220 1251
Size: 679 541 31
Fragments arranged by size:
679 541 31
MspA1I CmG'CkG
Cuts at: 0 301 340 1251
Size: 301 39 911
Fragments arranged by size:
911 301 39
NaeI GCC'GGC
Cuts at: 0 78 107 321 1251
Size: 78 29 214 930
Fragments arranged by size:
930 214 78 29
NarI GG'CG_CC
Cuts at: 0 119 126 218 413 1251
Size: 119 7 92 195 838
Fragments arranged by size:
838 195 119 92 7
NgoMIV G'CCGG_C
Cuts at: 0 76 105 319 1251
Size: 76 29 214 932
Fragments arranged by size:
932 214 76 29
NotI GC'GGCC_GC
Cuts at: 0 472 1251
Size: 472 779
NspI r_CATG'y
Cuts at: 0 1019 1251
Size: 1019 232
PflMI CCAn_nnn'nTGG
Cuts at: 0 891 1251
Size: 891 360
PpuMI rG'GwC_Cy
Cuts at: 0 210 1251
Size: 210 1041
SacII CC_GC'GG
Cuts at: 0 302 1251
Size: 302 949
SanDI GG'GwC_CC
Cuts at: 0 210 1251
Size: 210 1041
ScaI AGT'ACT
Cuts at: 0 529 1251
Size: 529 722
SexAI A'CCwGG_T
Cuts at: 0 641 1151 1251
Size: 641 510 100
Fragments arranged by size:
641 510 100
SfoI GGC'GCC
Cuts at: 0 120 127 219 414 1251
Size: 120 7 92 195 837
Fragments arranged by size:
837 195 120 92 7
SgrAI Cr'CCGG_yG
Cuts at: 0 105 1251
Size: 105 1146
SmaI CCC'GGG
Cuts at: 0 215 1251
Size: 215 1036
StyI C'CwwG_G
Cuts at: 0 813 1251
Size: 813 438
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 946 1251
Size: 946 305
TatI w'GTAC_w
Cuts at: 0 527 698 739 1018 1251
Size: 527 171 41 279 233
Fragments arranged by size:
527 279 233 171 41
XbaI T'CTAG_A
Cuts at: 0 394 1251
Size: 394 857
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 851 1251
Size: 851 400
XmaI C'CCGG_G
Cuts at: 0 213 1251
Size: 213 1038
Enzymes that do cut:
AarI Acc65I AcuI AleI ApaLI AvaI BanI BanII
BbeI BbsI BbvCI BciVI BclI BlpI Bme1580I BpmI
Bpu10I BsaI BsaAI BsaBI BsaHI BseRI BseYI BsgI
BsiEI BsiHKAI BsiWI Bsp1286I BspMI BsrFI BsrGI BssHII
BstAPI BstXI BstYI Bsu36I BtgI BtsI DraIII EaeI
EagI EciI Eco57MI EcoO109I HaeII KasI KpnI MscI
MslI MspA1I NaeI NarI NgoMIV NotI NspI PflMI
PpuMI SacII SanDI ScaI SexAI SfoI SgrAI SmaI
StyI TaqII TatI XbaI XcmI XmaI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AflIII AgeI AhdI
AlfI AloI AlwNI ApaI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BcgI BfrBI BglI BglII BmgBI
BmrI BmtI BplI BpuEI BsaWI BsaXI BsmI BsmBI
BspEI BspHI BsrBI BsrDI BssSI BstBI BstEII BstZ17I
BtgZI ClaI CspCI DraI DrdI EarI EcoICRI EcoNI
EcoRI EcoRV FalI FseI FspI FspAI Hin4I HincII
HindIII HpaI MfeI MluI MmeI NcoI NdeI NheI
NruI NsiI PacI PasI PciI PfoI PmeI PmlI
PpiI PshAI PsiI PspOMI PspXI PsrI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI SfcI SfiI
SgfI SmlI SnaBI SpeI SphI SrfI SspI StuI
SwaI TstI Tth111I XhoI XmnI ZraI