Restriction map for
pFN21AB0951
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# NO CUT ENZYMES ( 176 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AviII AvrII BalI BamHI BanII
BbeI BbrPI BbvCI BclI BfrI
BglI BlnI BlpI BmyI Bpu1102I
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BseRI BsgI BsiEI BsiHKAI
BsiWI BsmAI BsmBI BsmFI Bsp1286I
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I CelII CfoI Cfr10I ClaI
CpoI DdeI DraI DraII DraIII
DrdI EaeI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRV EcoT22I FauI
FbaI FseI FspI HaeII HhaI
Hin1I HinP1I HincII HindII HindIII
HpaI KasI KpnI Ksp632I KspI
MaeII MamI MfeI MluI MluNI
MroI MscI MseI MunI MvnI
MwoI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tru9I Tth111I Van91I XbaI XcmI
XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AcsI 513 GAATTC [GA]^AATT[CT]
AlwI 106 GATCC GGATC(4/5)
ApoI 513 GAATTC [GA]^AATT[CT]
AvaII 231 GGTCC G^G[AT]CC
BanI 333 GGTGCC G^G[CT][GA]CC
BbsI 54 GAAGAC GAAGAC(2/6)
BbvI 536 GCTGC GCAGC(8/12)
BcgI 525 GCAAGAGCGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 37 GGATAC GTATCC(6/5)
BglII 363 AGATCT A^GATCT
BpmI 371 CTCCAG CTGGAG(16/14)
BpuAI 54 GAAGAC GAAGAC(2/6)
BsiYI 165 CCTACATCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 165 CCTACATCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmI 203 GCATTC GAATGC(1/-1)
BsrI 337 CCAGT ACTGG(1/-1)
BstYI 363 AGATCT [GA]^GATC[CT]
Cac8I 408 GCTGGC GC[ACGT]^[ACGT]GC
Cfr13I 231 GGTCC G^G[ACGT]CC
EcoRI 513 GAATTC G^AATTC
HaeIII 316 GGCC GG^CC
HapII 330 CCGG C^CGG
HgaI 531 GCGTC GACGC(5/10)
HpaII 330 CCGG C^CGG
MflI 363 AGATCT [GA]^GATC[CT]
MslI 235 CATGGTTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 330 CCGG C^CGG
PaeR7I 449 CTCGAG C^TCGAG
PvuII 460 CAGCTG CAG^CTG
Sau96I 231 GGTCC G^G[ACGT]CC
SfaNI 473 GCATC GCATC(5/9)
SmaI 329 CCCGGG CCC^GGG
TaqI 450 TCGA T^CGA
TfiI 456 GAATC G^A[AT]TC
TseI 536 GCTGC G^C[AT]GC
TthHB8I 450 TCGA T^CGA
XhoI 449 CTCGAG C^TCGAG
XhoII 363 AGATCT [GA]^GATC[CT]
XmaCI 329 CCCGGG C^CCGGG
XmaI 329 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AvaI 329 CCCGGG C^[CT]CG[GA]G
449 CTCGAG
BcnI 329 CCCGG CC^[GC]GG
330 CCGGG
BfaI 7 CTAG C^TAG
346 CTAG
BsoBI 329 CCCGGG C^[CT]CG[GA]G
449 CTCGAG
BstNI 419 CCTGG CC^[AT]GG
492 CCAGG
DpnII 106 GATC ^GATC
364 GATC
DsaI 234 CCATGG C^C[GA][CT]GG
318 CCATGG
EcoRII 419 CCTGG ^CC[AT]GG
492 CCAGG
EcoT14I 234 CCATGG C^C[AT][AT]GG
318 CCATGG
Fnu4HI 3 GCCGC GC^[ACGT]GC
536 GCTGC
ItaI 3 GCCGC GC^[ACGT]GC
536 GCTGC
MaeI 7 CTAG C^TAG
346 CTAG
MaeIII 139 GTGAC ^GT[ACGT]AC
259 GTCAC
MboI 106 GATC ^GATC
364 GATC
MboII 54 GAAGA GAAGA(8/7)
476 TCTTC
MspA1I 42 CCGCTG C[AC]G^C[GT]G
460 CAGCTG
MvaI 419 CCTGG CC^[AT]GG
492 CCAGG
NciI 329 CCCGG CC^[GC]GG
330 CCGGG
NcoI 234 CCATGG C^CATGG
318 CCATGG
NdeII 106 GATC ^GATC
364 GATC
NlaIV 230 GGGTCC GG[ACGT]^[ACGT]CC
333 GGTGCC
Sau3AI 106 GATC ^GATC
364 GATC
SfcI 15 CTACAG C^T[GA][CT]AG
110 CTACAG
SmlI 449 CTCGAG C^T[CT][GA]AG
467 CTCAAG
StyI 234 CCATGG C^C[AT][AT]GG
318 CCATGG
Tsp45I 139 GTGAC ^GT[GC]AC
259 GTCAC
Tsp509I 74 AATT ^AATT
514 AATT
TspRI 113 CAGTG CA[GC]TG[ACGT][ACGT]^
338 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 14 enzymes) #
#########################################
AciI AfaI AluI BsaJI BssKI
BstF5I FokI HinfI HphI MnlI
NlaIII PleI RsaI ScrFI