Restriction map for
pFN21AB0948
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# NO CUT ENZYMES ( 157 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcsI AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp718I AspEI AspI
AvaI AviII AvrII BalI BamHI
BanII BbvCI BcgI BciVI BclI
BcnI BglI BglII BlnI BlpI
Bpu1102I BsaBI BsaWI BseAI BseRI
BsiEI BsiWI BsmBI BsmFI BsoBI
Bsp1407I BspDI BspEI BspLU11I BspMI
BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstF5I BstPI BstUI
BstXI Bsu36I CelII Cfr10I ClaI
CpoI DraI DraII DraIII DrdI
DsaI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRV EcoT14I FbaI
FokI FseI FspI HapII HincII
HindII HindIII HpaI HpaII KpnI
KspI MamI MfeI MluI MluNI
MroI MscI MspA1I MspI MunI
MvnI NaeI NciI NcoI NgoMIV
NheI NotI NruI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmeI PpuMI PshAI PshBI Psp1406I
PstI PvuI PvuII RsrII SacI
SacII SalI SbfI ScaI SexAI
SfcI SfiI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StyI SwaI TaqI Tth111I
TthHB8I Van91I XbaI XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AccI 215 GTATAC GT^[AC][GT]AC
AcyI 24 GGCGCC G[GA]^CG[CT]C
AfaI 117 GTAC GT^AC
AflII 900 CTTAAG C^TTAAG
AflIII 211 ACGTGT A^C[GA][CT]GT
AlwI 722 GATCC GGATC(4/5)
AlwNI 1041 CAGTGACTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 818 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 207 GAGCAC G[AT]GC[AT]^C
AvaII 905 GGTCC G^G[AT]CC
BanI 24 GGCGCC G^G[CT][GA]CC
BbeI 24 GGCGCC GGCGC^C
BbrPI 210 CACGTG CAC^GTG
BbsI 89 GTCTTC GAAGAC(2/6)
BfrI 900 CTTAAG C^TTAAG
BmyI 207 GAGCAC G[AGT]GC[ACT]^C
BpuAI 89 GTCTTC GAAGAC(2/6)
BsaAI 210 CACGTG [CT]AC^GT[GA]
BsaHI 24 GGCGCC G[GA]^CG[CT]C
BsaI 136 GAGACC GGTCTC(1/5)
BsiHKAI 207 GAGCAC G[AT]GC[AT]^C
BsiYI 14 CCGCTCTGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 14 CCGCTCTGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
Bsp1286I 207 GAGCAC G[AGT]GC[ACT]^C
BspHI 417 TCATGA T^CATGA
BsrBI 14 CCGCTC CCGCTC(-3/-3)
BsrDI 623 GCAATG GCAATG(2/0)
Bst1107I 215 GTATAC GTA^TAC
BstYI 721 AGATCC [GA]^GATC[CT]
BstZ17I 215 GTATAC GTA^TAC
CfoI 25 GCGC GCG^C
Cfr13I 905 GGTCC G^G[ACGT]CC
EcoT22I 1122 ATGCAT ATGCA^T
FauI 956 GCGGG CCCGC(4/6)
HaeII 24 GGCGCC [GA]GCGC^[CT]
HgaI 501 GCGTC GACGC(5/10)
HhaI 25 GCGC GCG^C
Hin1I 24 GGCGCC G[GA]^CG[CT]C
HinP1I 25 GCGC G^CGC
KasI 24 GGCGCC G^GCGCC
MflI 721 AGATCC [GA]^GATC[CT]
NarI 24 GGCGCC GG^CGCC
NdeI 1115 CATATG CA^TATG
NlaIV 24 GGCGCC GG[ACGT]^[ACGT]CC
NsiI 1122 ATGCAT ATGCA^T
PleI 87 GAGTC GAGTC(4/5)
PmaCI 210 CACGTG CAC^GTG
PmlI 210 CACGTG CAC^GTG
RcaI 417 TCATGA T^CATGA
RsaI 117 GTAC GT^AC
Sau96I 905 GGTCC G^G[ACGT]CC
SfoI 24 GGCGCC GGC^GCC
TfiI 678 GATTC G^A[AT]TC
XhoII 721 AGATCC [GA]^GATC[CT]
XmnI 818 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 9 enzymes) #
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Cac8I 27 GCCTGC GC[ACGT]^[ACGT]GC
956 GCGGGC
DpnII 325 GATC ^GATC
722 GATC
HinfI 87 GAGTC G^A[ACGT]TC
678 GATTC
MaeII 125 ACGT A^CGT
211 ACGT
MboI 325 GATC ^GATC
722 GATC
NdeII 325 GATC ^GATC
722 GATC
SapI 485 GCTCTTC GCTCTTC(1/4)
821 GCTCTTC
Sau3AI 325 GATC ^GATC
722 GATC
Tsp509I 284 AATT ^AATT
386 AATT
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# 3 OR MORE CUT ENZYMES ( 37 enzymes) #
#########################################
AciI AluI BbvI BfaI BpmI
BsaJI BsgI BsmAI BsmI BsrI
BssKI BstNI DdeI EarI EcoRII
Fnu4HI HaeIII HphI ItaI Ksp632I
MaeI MaeIII MboII MnlI MseI
MslI MvaI MwoI NlaIII ScrFI
SfaNI SmlI StuI Tru9I TseI
Tsp45I TspRI