Restriction map for
pFN21AB0948
(Circular) (Six-base) MAPSORT of: pFN21AB0948.seq Check: 6653 from: 1 to: 5911
pFN21AB0948 5911 bp
With 182 enzymes: SgfI *
April 15, 2010 09:33 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3721 278
Size: 53 83 186 936 2185 2468
Fragments arranged by size:
2468 2185 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2221 3254 1937
Size: 284 1033 4594
Fragments arranged by size:
4594 1033 284
Acc65I G'GTAC_C
Cuts at: 3232 3232
Size: 5911
AclI AA'CG_TT
Cuts at: 1563 4038 4411 1563
Size: 2475 373 3063
Fragments arranged by size:
3063 2475 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3974 5358 5626 1632
Size: 2342 1384 268 1917
Fragments arranged by size:
2342 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 2905 820
Size: 197 1888 3826
Fragments arranged by size:
3826 1888 197
AflIII A'CryG_T
Cuts at: 1203 2216 4810 5787 1203
Size: 1013 2594 977 1327
Fragments arranged by size:
2594 1327 1013 977
AgeI A'CCGG_T
Cuts at: 4742 4742
Size: 5911
AhdI GACnn_n'nnGTC
Cuts at: 4640 4640
Size: 5911
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5911
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5911
AlwNI CAG_nnn'CTG
Cuts at: 3051 3654 5226 3051
Size: 603 1572 3736
Fragments arranged by size:
3736 1572 603
ApaLI G'TGCA_C
Cuts at: 3968 5124 3968
Size: 1156 4755
ApoI r'AATT_y
Cuts at: 1493 3220 3446 3598 5707 1493
Size: 1727 226 152 2109 1697
Fragments arranged by size:
2109 1727 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4465 160
Size: 872 3433 1606
Fragments arranged by size:
3433 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5911
AvaI C'yCGr_G
Cuts at: 1870 1943 3236 1870
Size: 73 1293 4545
Fragments arranged by size:
4545 1293 73
BamHI G'GATC_C
Cuts at: 3241 3608 3241
Size: 367 5544
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2029 3232 4687 618
Size: 325 217 627 242 1203 1455 1842
Fragments arranged by size:
1842 1455 1203 627 325 242 217
BanII G_rGCy'C
Cuts at: 1391 3230 1391
Size: 1839 4072
BbeI G_GCGC'C
Cuts at: 2033 2033
Size: 5911
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2087 928
Size: 971 188 4752
Fragments arranged by size:
4752 971 188
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4102 4136 1903
Size: 34 2165 34 3678
Fragments arranged by size:
3678 2165 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3803 5013 3803
Size: 1210 4701
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5911
BfrBI ATG'CAT
Cuts at: 3129 3129
Size: 5911
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 4522 136
Size: 107 122 71 631 605 337 2513 1525
Fragments arranged by size:
2513 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 5906 5906
Size: 5911
BlpI GC'TnA_GC
Cuts at: 3328 3328
Size: 5911
Bme1580I G_kGCm'C
Cuts at: 1790 3972 5128 1790
Size: 2182 1156 2573
Fragments arranged by size:
2573 2182 1156
BmgBI CAC'GTC
Cuts at: 1318 2131 1318
Size: 813 5098
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2448 3642 4600
Size: 416 53 83 399 24 1031 1194 958
Cuts at: 4600 442
Size: 1753
Fragments arranged by size:
1753 1194 1031 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5911
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2102 2159 2376 2628 2712 2728 2964 3045
Size: 863 57 217 252 84 16 236 81
Cuts at: 3045 3061 3129 4571 1239
Size: 16 68 1442 2579
Fragments arranged by size:
2579 1442 863 252 236 217 84 81
68 57 16 16
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2782 3115 4036 4901 5199 2782
Size: 333 921 865 298 3494
Fragments arranged by size:
3494 921 865 333 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2135 4574 882
Size: 617 636 2439 2219
Fragments arranged by size:
2439 2219 636 617
BsaAI yAC'GTr
Cuts at: 493 1204 2217 3702 493
Size: 711 1013 1485 2702
Fragments arranged by size:
2702 1485 1013 711
BsaBI GATnn'nnATC
Cuts at: 5905 5905
Size: 5911
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2030 3718 4100 4735
Size: 53 83 186 936 497 1688 382 635
Cuts at: 4735 275
Size: 1451
Fragments arranged by size:
1688 1451 936 635 497 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4344 4742 5016 5163 5574 4344
Size: 398 274 147 411 4681
Fragments arranged by size:
4681 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5686
Fragments arranged by size:
5686 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5911
BseYI C'CCAG_C
Cuts at: 5114 5610 5114
Size: 496 5415
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2395 2479 2731 3064 1163
Size: 573 659 84 252 333 4010
Fragments arranged by size:
4010 659 573 333 252 84
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3361 4122 5150 1540
Size: 275 186 1360 761 1028 2301
Fragments arranged by size:
2301 1360 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2216 3972 4057 5128 2216
Size: 1756 85 1071 2999
Fragments arranged by size:
2999 1756 1071 85
BsmI GAATG_Cn'
Cuts at: 2630 2798 3131 3422 3515 2630
Size: 168 333 291 93 5026
Fragments arranged by size:
5026 333 291 168 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2216 3230 3972 4057 5128 1391
Size: 399 426 1014 742 85 1071 2174
Fragments arranged by size:
2174 1071 1014 742 426 399 85
BspEI T'CCGG_A
Cuts at: 5574 5574
Size: 5911
BspHI T'CATG_A
Cuts at: 2422 3800 5530 2422
Size: 1378 1730 2803
Fragments arranged by size:
2803 1730 1378
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3266 844
Size: 2422 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2021 3798 1394
Size: 429 198 1777 3507
Fragments arranged by size:
3507 1777 429 198
BsrDI GCAATG_nn'
Cuts at: 65 1248 2635 4406 4580 65
Size: 1183 1387 1771 174 1396
Fragments arranged by size:
1771 1396 1387 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4555 4742 1700
Size: 110 2745 187 2869
Fragments arranged by size:
2869 2745 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5911
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5911
BssSI C'ACGA_G
Cuts at: 3971 4983 3971
Size: 1012 4899
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5911
BstBI TT'CG_AA
Cuts at: 4715 4715
Size: 5911
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5911
BstYI r'GATC_y
Cuts at: 1972 2726 3241 3608 3993 4010 5462 5548 5560
Size: 754 515 367 385 17 1452 86 12
Cuts at: 5560 5906 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 754 515 385 367 346 86
17 12
BstZ17I GTA'TAC
Cuts at: 2222 2222
Size: 5911
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4420
Fragments arranged by size:
4420 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5172
Fragments arranged by size:
5172 683 56
BtsI GCAGTG_nn'
Cuts at: 2050 2467 2719 2972 3052 3427 4221 4241 2050
Size: 417 252 253 80 375 794 20 3720
Fragments arranged by size:
3720 794 417 375 253 252 80 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5911
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2617 2652 529
Size: 35 2053 35 3788
Fragments arranged by size:
3788 2053 35 35
DraI TTT'AAA
Cuts at: 3215 3215
Size: 5911
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4918 809
Size: 507 3602 1802
Fragments arranged by size:
3602 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3358 4247 5876
Size: 54 1425 50 275 18 1528 889 1629
Cuts at: 5876 8
Size: 43
Fragments arranged by size:
1629 1528 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3358 1537
Size: 275 1546 4090
Fragments arranged by size:
4090 1546 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2320 2497 2833 3841 1353
Size: 967 177 336 1008 3423
Fragments arranged by size:
3423 1008 967 336 177
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4498 4872 5018 387
Size: 1064 138 2909 374 146 1280
Fragments arranged by size:
2909 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2102 2159 2376 2628 2712 2728 2964
Size: 393 470 57 217 252 84 16 236
Cuts at: 2964 3045 3061 3129 3974 4571 5358 5626 1239
Size: 81 16 68 845 597 787 268 1524
Fragments arranged by size:
1524 845 787 597 470 393 268 252
236 217 84 81 68 57 16 16
EcoRI G'AATT_C
Cuts at: 3220 3598 3220
Size: 378 5533
FspI TGC'GCA
Cuts at: 4417 4417
Size: 5911
HaeII r_GCGC'y
Cuts at: 1678 2033 5058 1678
Size: 355 3025 2531
Fragments arranged by size:
3025 2531 355
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4552 4584 4626 4658 1373
Size: 32 135 32 2980 32 42 32 2626
Fragments arranged by size:
2980 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3255 3501 669
Size: 988 281 1317 246 3079
Fragments arranged by size:
3079 1317 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5911
HpaI GTT'AAC
Cuts at: 3501 3501
Size: 5911
KasI G'GCGC_C
Cuts at: 2029 2029
Size: 5911
KpnI G_GTAC'C
Cuts at: 3236 3236
Size: 5911
MfeI C'AATT_G
Cuts at: 3510 3510
Size: 5911
MluI A'CGCG_T
Cuts at: 5787 5787
Size: 5911
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2937 5025 5209 1305
Size: 1632 2088 184 2007
Fragments arranged by size:
2088 2007 1632 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4089
Fragments arranged by size:
4089 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2343 2421 3124 3869 4228 4387 518
Size: 648 1177 78 703 745 359 159 2042
Fragments arranged by size:
2042 1177 745 703 648 359 159 78
MspA1I CmG'CkG
Cuts at: 1606 3327 4004 5152 5397 1606
Size: 1721 677 1148 245 2120
Fragments arranged by size:
2120 1721 1148 677 245
NarI GG'CG_CC
Cuts at: 2030 2030
Size: 5911
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4420
Fragments arranged by size:
4420 939 552
NdeI CA'TA_TG
Cuts at: 387 3121 387
Size: 2734 3177
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5911
NotI GC'GGCC_GC
Cuts at: 3358 3358
Size: 5911
NsiI A_TGCA'T
Cuts at: 3131 3131
Size: 5911
NspI r_CATG'y
Cuts at: 1132 1229 3269 4814 1132
Size: 97 2040 1545 2229
Fragments arranged by size:
2229 2040 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2164 1348
Size: 816 5095
PciI A'CATG_T
Cuts at: 4810 4810
Size: 5911
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5911
PmeI GTTT'AAAC
Cuts at: 3215 3215
Size: 5911
PmlI CAC'GTG
Cuts at: 2217 2217
Size: 5911
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3980 4012 5493 5525 3980
Size: 32 1481 32 4366
Fragments arranged by size:
4366 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5911
PsiI TTA'TAA
Cuts at: 3207 3481 3207
Size: 274 5637
PstI C_TGCA'G
Cuts at: 830 3263 830
Size: 2433 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5911
SalI G'TCGA_C
Cuts at: 1936 3253 1936
Size: 1317 4594
SapI GCTCTTCn'nnn_
Cuts at: 2497 2833 2497
Size: 336 5575
SbfI CC_TGCA'GG
Cuts at: 3263 3263
Size: 5911
ScaI AGT'ACT
Cuts at: 4159 5871 4159
Size: 1712 4199
SfcI C'TryA_G
Cuts at: 826 1045 3259 4394 5075 5266 826
Size: 219 2214 1135 681 191 1471
Fragments arranged by size:
2214 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2031 2031
Size: 5911
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5911
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5911
SmaI CCC'GGG
Cuts at: 1872 3238 1872
Size: 1366 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2761 2905 3094 4015 4916 5178
Size: 197 926 818 144 189 921 901 262
Cuts at: 5178 820
Size: 1553
Fragments arranged by size:
1553 926 921 901 818 262 197 189
144
SnaBI TAC'GTA
Cuts at: 493 3702 493
Size: 3209 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5911
SphI G_CATG'C
Cuts at: 1132 3269 1132
Size: 2137 3774
SspI AAT'ATT
Cuts at: 5 52 3835 5
Size: 47 3783 2081
Fragments arranged by size:
3783 2081 47
StuI AGG'CCT
Cuts at: 1517 2263 2365 2533 2701 2785 2869 3034 3202
Size: 746 102 168 168 84 84 165 168
Cuts at: 3202 1517
Size: 4226
Fragments arranged by size:
4226 746 168 168 168 165 102 84
84
StyI C'CwwG_G
Cuts at: 513 1065 2004 3350 513
Size: 552 939 1346 3074
Fragments arranged by size:
3074 1346 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3938 3955 4108 4293 4738 325
Size: 1255 2358 17 153 185 445 1498
Fragments arranged by size:
2358 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4157 5869 96
Size: 274 80 33 51 1443 2180 1712 138
Fragments arranged by size:
2180 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5871 5903 5871
Size: 32 5879
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5911
XbaI T'CTAG_A
Cuts at: 3247 3247
Size: 5911
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5911
XmaI C'CCGG_G
Cuts at: 1870 3236 1870
Size: 1366 4545
XmnI GAAnn'nnTTC
Cuts at: 2827 4040 2827
Size: 1213 4698
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3719 276
Size: 53 83 186 936 2185 2468
Fragments arranged by size:
2468 2185 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NarI NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI
PstI PvuI SalI SapI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BplI Bpu10I BsiWI BsmBI BstXI Bsu36I DraIII EcoICRI
EcoNI EcoO109I EcoRV FalI FseI FspAI NaeI NgoMIV
NruI PacI PfoI PpuMI PspOMI PspXI PsrI PvuII
RsrII SacI SacII SanDI SexAI SfiI SrfI SwaI
XcmI