Restriction map for
pFN21AB0943
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# NO CUT ENZYMES ( 161 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AfaI AflII
AflIII AgeI AhdI AluI AocI
Aor51HI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvaI
AviII BamHI BanI BbeI BbrPI
BbvCI BcgI BciVI BclI BfrI
BglII BlpI Bpu1102I BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BseRI
BsiEI BsiWI BsmBI BsmFI BsmI
BsoBI Bsp1407I BspDI BspEI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrGI
BssHII Bst1107I BstAPI BstEII BstPI
BstUI BstXI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HaeII
HgaI Hin1I HincII HindII HindIII
HpaI HphI KasI KpnI KspI
MaeII MaeIII MamI MfeI MluI
MroI MspA1I MunI MvnI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PaeR7I
PflFI PinAI PleI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI PvuII RsaI RsrII SacI
SacII SalI SapI SbfI ScaI
SfiI SfoI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Tsp45I Tth111I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
###################################
Alw44I 133 GTGCAC G^TGCAC
AlwI 495 GGATC GGATC(4/5)
ApaI 415 GGGCCC GGGCC^C
ApaLI 133 GTGCAC G^TGCAC
AvrII 51 CCTAGG C^CTAGG
BbsI 184 GAAGAC GAAGAC(2/6)
BcnI 241 CCCGG CC^[GC]GG
BlnI 51 CCTAGG C^CTAGG
BpmI 328 CTGGAG CTGGAG(16/14)
BpuAI 184 GAAGAC GAAGAC(2/6)
BsgI 43 CTGCAC GTGCAG(16/14)
BsmAI 10 GTCTC GTCTC(1/5)
BsrI 283 ACTGG ACTGG(1/-1)
BssSI 299 CACGAG CACGAG(-5/-1)
BstBI 103 TTCGAA TT^CGAA
BstYI 495 GGATCT [GA]^GATC[CT]
CfoI 424 GCGC GCG^C
DpnII 496 GATC ^GATC
DsaI 92 CCATGG C^C[GA][CT]GG
EarI 79 CTCTTC CTCTTC(1/4)
EcoNI 383 CCTTCCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HapII 242 CCGG C^CGG
HhaI 424 GCGC GCG^C
HinP1I 424 GCGC G^CGC
HinfI 59 GATTC G^A[ACGT]TC
HpaII 242 CCGG C^CGG
Ksp632I 79 CTCTTC CTCTTC(1/4)
MboI 496 GATC ^GATC
MflI 495 GGATCT [GA]^GATC[CT]
MseI 341 TTAA T^TAA
MslI 124 CATGGCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 242 CCGG C^CGG
NciI 241 CCCGG CC^[GC]GG
NcoI 92 CCATGG C^CATGG
NdeII 496 GATC ^GATC
NspV 103 TTCGAA TT^CGAA
PpuMI 163 AGGACCT [GA]G^G[AT]CC[CT]
Sau3AI 496 GATC ^GATC
SexAI 204 ACCTGGT A^CC[AT]GGT
SfcI 14 CTGTAG C^T[GA][CT]AG
SfuI 103 TTCGAA TT^CGAA
TfiI 59 GATTC G^A[AT]TC
Tru9I 341 TTAA T^TAA
XhoII 495 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AlwNI 8 CAGTCTCTG CAG[ACGT][ACGT][ACGT]^CTG
200 CAGAACCTG
AvaII 164 GGACC G^G[AT]CC
472 GGACC
BalI 217 TGGCCA TGG^CCA
255 TGGCCA
BanII 264 GGGCTC G[GA]GC[CT]^C
415 GGGCCC
BfaI 52 CTAG C^TAG
116 CTAG
BglI 248 GCCATCTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
257 GCCAAGTGGGC
BspHI 20 TCATGA T^CATGA
150 TCATGA
BstF5I 32 CATCC GGATG(2/0)
48 CATCC
Cac8I 393 GCCCGC GC[ACGT]^[ACGT]GC
397 GCCCGC
DraII 163 AGGACCT [GA]G^G[ACGT]CC[CT]
415 GGGCCCC
EcoO109I 163 AGGACCT [GA]G^G[ACGT]CC[CT]
415 GGGCCCC
FokI 32 CATCC GGATG(9/13)
48 CATCC
MaeI 52 CTAG C^TAG
116 CTAG
MluNI 217 TGGCCA TGG^CCA
255 TGGCCA
MnlI 358 CCTC CCTC(7/6)
302 GAGG
MscI 217 TGGCCA TGG^CCA
255 TGGCCA
PflMI 226 CCATCTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
475 CCACCTTATGG
RcaI 20 TCATGA T^CATGA
150 TCATGA
TaqI 104 TCGA T^CGA
291 TCGA
TspRI 214 CAGTG CA[GC]TG[ACGT][ACGT]^
501 CACTG
TthHB8I 104 TCGA T^CGA
291 TCGA
Van91I 226 CCATCTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
475 CCACCTTATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 31 enzymes) #
#########################################
AciI AspHI BbvI BmyI BsaJI
BsiHKAI BsiYI BslI Bsp1286I BssKI
BstNI Cfr13I DdeI EaeI EcoRII
EcoT14I FauI Fnu4HI HaeIII ItaI
MboII MvaI MwoI NlaIII NlaIV
Sau96I ScrFI SfaNI StyI TseI
Tsp509I