Restriction map for
pFN21AB0943
(Linear) (Six-base) MAPSORT of: KIBB0943.seq Check: 3606 from: 1 to: 510
KIBB0943.orf
With 182 enzymes: SgfI *
October 7, 2009 14:15 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 203 510
Size: 203 307
AlwNI CAG_nnn'CTG
Cuts at: 0 13 205 510
Size: 13 192 305
Fragments arranged by size:
305 192 13
ApaI G_GGCC'C
Cuts at: 0 419 510
Size: 419 91
ApaLI G'TGCA_C
Cuts at: 0 133 510
Size: 133 377
AvrII C'CTAG_G
Cuts at: 0 51 510
Size: 51 459
BanII G_rGCy'C
Cuts at: 0 268 419 510
Size: 268 151 91
Fragments arranged by size:
268 151 91
BbsI GAAGACnn'nnnn_
Cuts at: 0 191 510
Size: 191 319
BglI GCCn_nnn'nGGC
Cuts at: 0 254 263 510
Size: 254 9 247
Fragments arranged by size:
254 247 9
Bme1580I G_kGCm'C
Cuts at: 0 137 419 510
Size: 137 282 91
Fragments arranged by size:
282 137 91
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 349 510
Size: 349 161
BseYI C'CCAG_C
Cuts at: 0 271 420 510
Size: 271 149 90
Fragments arranged by size:
271 149 90
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 28 510
Size: 28 482
BsiHKAI G_wGCw'C
Cuts at: 0 137 213 297 377 510
Size: 137 76 84 80 133
Fragments arranged by size:
137 133 84 80 76
Bsp1286I G_dGCh'C
Cuts at: 0 137 213 268 297 377 419 510
Size: 137 76 55 29 80 42 91
Fragments arranged by size:
137 91 80 76 55 42 29
BspHI T'CATG_A
Cuts at: 0 20 150 510
Size: 20 130 360
Fragments arranged by size:
360 130 20
BssSI C'ACGA_G
Cuts at: 0 299 510
Size: 299 211
BstBI TT'CG_AA
Cuts at: 0 104 510
Size: 104 406
BstYI r'GATC_y
Cuts at: 0 495 510
Size: 495 15
BtgI C'CryG_G
Cuts at: 0 92 510
Size: 92 418
BtsI GCAGTG_nn'
Cuts at: 0 500 510
Size: 500 10
EaeI y'GGCC_r
Cuts at: 0 126 217 255 510
Size: 126 91 38 255
Fragments arranged by size:
255 126 91 38
EarI CTCTTCn'nnn_
Cuts at: 0 85 510
Size: 85 425
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 203 349 510
Size: 203 146 161
Fragments arranged by size:
203 161 146
EcoNI CCTnn'n_nnAGG
Cuts at: 0 387 510
Size: 387 123
EcoO109I rG'GnC_Cy
Cuts at: 0 164 416 510
Size: 164 252 94
Fragments arranged by size:
252 164 94
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 245 510
Size: 245 265
MscI TGG'CCA
Cuts at: 0 219 257 510
Size: 219 38 253
Fragments arranged by size:
253 219 38
MslI CAynn'nnrTG
Cuts at: 0 128 510
Size: 128 382
NcoI C'CATG_G
Cuts at: 0 92 510
Size: 92 418
PflMI CCAn_nnn'nTGG
Cuts at: 0 232 481 510
Size: 232 249 29
Fragments arranged by size:
249 232 29
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 27 510
Size: 27 483
PpuMI rG'GwC_Cy
Cuts at: 0 164 510
Size: 164 346
PspOMI G'GGCC_C
Cuts at: 0 415 510
Size: 415 95
SexAI A'CCwGG_T
Cuts at: 0 204 510
Size: 204 306
SfcI C'TryA_G
Cuts at: 0 14 510
Size: 14 496
StyI C'CwwG_G
Cuts at: 0 51 92 220 388 510
Size: 51 41 128 168 122
Fragments arranged by size:
168 128 122 51 41
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 174 510
Size: 174 336
Enzymes that do cut:
AcuI AlwNI ApaI ApaLI AvrII BanII BbsI BglI
Bme1580I BpmI BseYI BsgI BsiHKAI Bsp1286I BspHI BssSI
BstBI BstYI BtgI BtsI EaeI EarI Eco57MI EcoNI
EcoO109I FalI MscI MslI NcoI PflMI PpiI PpuMI
PspOMI SexAI SfcI StyI TaqII
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApoI AscI AseI
AsiSI AvaI BaeI BamHI BanI BbeI BbvCI BcgI
BciVI BclI BfrBI BglII BlpI BmgBI BmrI BmtI
BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BsiEI BsiWI BsmI BsmBI BspEI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BstAPI BstEII BstXI
BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI
EagI EciI EcoICRI EcoRI EcoRV FseI FspI FspAI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MspA1I NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI PasI PciI PfoI
PmeI PmlI PshAI PsiI PspXI PsrI PstI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI SwaI TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI