(Circular) (Six-base) MAPSORT of: pFN21AB0942.seq Check: 269 from: 1 to: 5881 pFN21AB0942 5881 bp With 182 enzymes: SgfI * April 15, 2010 09:33 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3691 278 Size: 53 83 186 936 2155 2468 Fragments arranged by size: 2468 2155 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3224 1937 Size: 1287 4594 Acc65I G'GTAC_C Cuts at: 3202 3202 Size: 5881 AclI AA'CG_TT Cuts at: 1563 4008 4381 1563 Size: 2445 373 3063 Fragments arranged by size: 3063 2445 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2372 3944 5328 5596 1632 Size: 740 1572 1384 268 1917 Fragments arranged by size: 1917 1572 1384 740 268 AflII C'TTAA_G Cuts at: 820 1017 2831 820 Size: 197 1814 3870 Fragments arranged by size: 3870 1814 197 AflIII A'CryG_T Cuts at: 1203 2754 2894 4780 5757 1203 Size: 1551 140 1886 977 1327 Fragments arranged by size: 1886 1551 1327 977 140 AgeI A'CCGG_T Cuts at: 4712 4712 Size: 5881 AhdI GACnn_n'nnGTC Cuts at: 4610 4610 Size: 5881 AleI CACnn'nnGTG Cuts at: 1166 2710 1166 Size: 1544 4337 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5881 AlwNI CAG_nnn'CTG Cuts at: 2150 2651 2666 3624 5196 2150 Size: 501 15 958 1572 2835 Fragments arranged by size: 2835 1572 958 501 15 ApaLI G'TGCA_C Cuts at: 3017 3938 5094 3017 Size: 921 1156 3804 Fragments arranged by size: 3804 1156 921 ApoI r'AATT_y Cuts at: 1493 2270 2622 2636 2723 2769 3190 3416 3568 Size: 777 352 14 87 46 421 226 152 Cuts at: 3568 5677 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 777 421 352 226 152 87 46 14 AseI AT'TA_AT Cuts at: 160 1032 4435 160 Size: 872 3403 1606 Fragments arranged by size: 3403 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5881 AvaI C'yCGr_G Cuts at: 1870 1943 2066 3206 1870 Size: 73 123 1140 4545 Fragments arranged by size: 4545 1140 123 73 BamHI G'GATC_C Cuts at: 3211 3578 3211 Size: 367 5514 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2600 3202 4657 618 Size: 325 217 627 813 602 1455 1842 Fragments arranged by size: 1842 1455 813 627 602 325 217 BanII G_rGCy'C Cuts at: 1391 2067 2236 3200 1391 Size: 676 169 964 4072 Fragments arranged by size: 4072 964 676 169 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4910 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4072 4106 1903 Size: 34 2135 34 3678 Fragments arranged by size: 3678 2135 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3773 4983 3773 Size: 1210 4671 BclI T'GATC_A Cuts at: 1548 1548 Size: 5881 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4492 136 Size: 107 122 71 631 605 2820 1525 Fragments arranged by size: 2820 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5876 5876 Size: 5881 BlpI GC'TnA_GC Cuts at: 3298 3298 Size: 5881 Bme1580I G_kGCm'C Cuts at: 1790 3021 3942 5098 1790 Size: 1231 921 1156 2573 Fragments arranged by size: 2573 1231 1156 921 BmgBI CAC'GTC Cuts at: 1318 2895 1318 Size: 1577 4304 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2184 3612 4570 Size: 416 53 83 399 24 767 1428 958 Cuts at: 4570 442 Size: 1753 Fragments arranged by size: 1753 1428 958 767 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5881 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2688 2931 4541 1239 Size: 1449 243 1610 2579 Fragments arranged by size: 2579 1610 1449 243 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4006 4871 5169 4006 Size: 865 298 4718 Fragments arranged by size: 4718 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4544 882 Size: 617 3045 2219 Fragments arranged by size: 3045 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3672 493 Size: 711 2468 2702 Fragments arranged by size: 2702 2468 711 BsaBI GATnn'nnATC Cuts at: 5875 5875 Size: 5881 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3688 4070 4705 275 Size: 53 83 186 936 2155 382 635 1451 Fragments arranged by size: 2155 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4314 4712 4986 5133 5544 4314 Size: 398 274 147 411 4651 Fragments arranged by size: 4651 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2117 2147 1178 Size: 30 165 30 714 30 4912 Fragments arranged by size: 4912 714 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2544 1186 Size: 1358 4523 BseYI C'CCAG_C Cuts at: 5084 5580 5084 Size: 496 5385 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5308 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3331 4092 5120 1540 Size: 275 186 1330 761 1028 2301 Fragments arranged by size: 2301 1330 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3021 3942 4027 5098 3021 Size: 921 85 1071 3804 Fragments arranged by size: 3804 1071 921 85 BsmI GAATG_Cn' Cuts at: 2351 2477 3392 3485 2351 Size: 126 915 93 4747 Fragments arranged by size: 4747 915 126 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2067 2236 3021 3200 3942 4027 5098 Size: 399 277 169 785 179 742 85 1071 Cuts at: 5098 1391 Size: 2174 Fragments arranged by size: 2174 1071 785 742 399 277 179 169 85 BspEI T'CCGG_A Cuts at: 5544 5544 Size: 5881 BspHI T'CATG_A Cuts at: 2734 3770 5500 2734 Size: 1036 1730 3115 Fragments arranged by size: 3115 1730 1036 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2023 3169 3236 844 Size: 1179 1146 67 3489 Fragments arranged by size: 3489 1179 1146 67 BsrBI CCG'CTC Cuts at: 1394 1823 2303 3768 1394 Size: 429 480 1465 3507 Fragments arranged by size: 3507 1465 480 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2096 4376 4550 65 Size: 1183 848 2280 174 1396 Fragments arranged by size: 2280 1396 1183 848 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4525 4712 1700 Size: 110 2715 187 2869 Fragments arranged by size: 2869 2715 187 110 BsrGI T'GTAC_A Cuts at: 96 2053 96 Size: 1957 3924 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5881 BssSI C'ACGA_G Cuts at: 2084 3941 4953 2084 Size: 1857 1012 3012 Fragments arranged by size: 3012 1857 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5881 BstBI TT'CG_AA Cuts at: 4685 4685 Size: 5881 BstEII G'GTnAC_C Cuts at: 1184 2380 1184 Size: 1196 4685 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2011 Size: 5881 BstYI r'GATC_y Cuts at: 1972 3211 3578 3963 3980 5432 5518 5530 5876 Size: 1239 367 385 17 1452 86 12 346 Cuts at: 5876 1972 Size: 1977 Fragments arranged by size: 1977 1452 1239 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4390 Fragments arranged by size: 4390 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5142 Fragments arranged by size: 5142 683 56 BtsI GCAGTG_nn' Cuts at: 2127 2583 2667 3397 4191 4211 2127 Size: 456 84 730 794 20 3797 Fragments arranged by size: 3797 794 730 456 84 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5881 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5846 DraI TTT'AAA Cuts at: 2728 3185 2728 Size: 457 5424 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4888 809 Size: 507 3572 1802 Fragments arranged by size: 3572 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3328 4217 5846 Size: 54 1425 50 275 18 1498 889 1629 Cuts at: 5846 8 Size: 43 Fragments arranged by size: 1629 1498 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3328 1537 Size: 275 1516 4090 Fragments arranged by size: 4090 1516 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3113 3143 3811 1353 Size: 1760 30 668 3423 Fragments arranged by size: 3423 1760 668 30 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4468 4842 4988 387 Size: 1064 138 2879 374 146 1280 Fragments arranged by size: 2879 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2372 2688 2931 3944 4541 5328 5596 Size: 393 740 316 243 1013 597 787 268 Cuts at: 5596 1239 Size: 1524 Fragments arranged by size: 1524 1013 787 740 597 393 316 268 243 EcoO109I rG'GnC_Cy Cuts at: 2035 2035 Size: 5881 EcoRI G'AATT_C Cuts at: 3190 3568 3190 Size: 378 5503 EcoRV GAT'ATC Cuts at: 3108 3108 Size: 5881 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2957 2957 Size: 5881 FspI TGC'GCA Cuts at: 4387 4387 Size: 5881 HaeII r_GCGC'y Cuts at: 1678 5028 1678 Size: 3350 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2573 2605 4522 4554 4596 Size: 32 135 32 1001 32 1917 32 42 Cuts at: 4596 4628 1373 Size: 32 2626 Fragments arranged by size: 2626 1917 1001 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3225 3471 669 Size: 988 281 1287 246 3079 Fragments arranged by size: 3079 1287 988 281 246 HindIII A'AGCT_T Cuts at: 748 2828 748 Size: 2080 3801 HpaI GTT'AAC Cuts at: 3471 3471 Size: 5881 KpnI G_GTAC'C Cuts at: 3206 3206 Size: 5881 MfeI C'AATT_G Cuts at: 3480 3480 Size: 5881 MluI A'CGCG_T Cuts at: 5757 5757 Size: 5881 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3120 4995 5179 1305 Size: 1815 1875 184 2007 Fragments arranged by size: 2007 1875 1815 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4059 Fragments arranged by size: 4059 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2202 2247 2710 2819 3839 4198 4357 Size: 648 1036 45 463 109 1020 359 159 Cuts at: 4357 518 Size: 2042 Fragments arranged by size: 2042 1036 1020 648 463 359 159 109 45 MspA1I CmG'CkG Cuts at: 1606 2951 3297 3974 5122 5367 1606 Size: 1345 346 677 1148 245 2120 Fragments arranged by size: 2120 1345 1148 677 346 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4390 Fragments arranged by size: 4390 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5881 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5881 NotI GC'GGCC_GC Cuts at: 3328 3328 Size: 5881 NspI r_CATG'y Cuts at: 1132 1229 2758 3239 4784 1132 Size: 97 1529 481 1545 2229 Fragments arranged by size: 2229 1545 1529 481 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5881 PciI A'CATG_T Cuts at: 2754 4780 2754 Size: 2026 3855 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5881 PmeI GTTT'AAAC Cuts at: 3185 3185 Size: 5881 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3950 3982 5463 5495 3950 Size: 32 1481 32 4336 Fragments arranged by size: 4336 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2035 2035 Size: 5881 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5881 PsiI TTA'TAA Cuts at: 2684 3451 2684 Size: 767 5114 PstI C_TGCA'G Cuts at: 830 2956 3233 830 Size: 2126 277 3478 Fragments arranged by size: 3478 2126 277 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5881 PvuII CAG'CTG Cuts at: 2951 2951 Size: 5881 SalI G'TCGA_C Cuts at: 1936 3223 1936 Size: 1287 4594 SbfI CC_TGCA'GG Cuts at: 3233 3233 Size: 5881 ScaI AGT'ACT Cuts at: 4129 5841 4129 Size: 1712 4169 SfcI C'TryA_G Cuts at: 826 1045 2952 3229 4364 5045 5236 826 Size: 219 1907 277 1135 681 191 1471 Fragments arranged by size: 1907 1471 1135 681 277 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5881 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5881 SmaI CCC'GGG Cuts at: 1872 3208 1872 Size: 1336 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2831 3985 4886 5148 820 Size: 197 926 888 1154 901 262 1553 Fragments arranged by size: 1553 1154 926 901 888 262 197 SnaBI TAC'GTA Cuts at: 493 3672 493 Size: 3179 2702 SpeI A'CTAG_T Cuts at: 152 2707 152 Size: 2555 3326 SphI G_CATG'C Cuts at: 1132 3239 1132 Size: 2107 3774 SspI AAT'ATT Cuts at: 5 52 3805 5 Size: 47 3753 2081 Fragments arranged by size: 3753 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5881 StyI C'CwwG_G Cuts at: 513 1065 2004 3320 513 Size: 552 939 1316 3074 Fragments arranged by size: 3074 1316 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2415 3908 3925 4078 4263 4708 325 Size: 1255 835 1493 17 153 185 445 1498 Fragments arranged by size: 1498 1493 1255 835 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2053 4127 5839 Size: 274 80 33 51 1443 76 2074 1712 Cuts at: 5839 96 Size: 138 Fragments arranged by size: 2074 1712 1443 274 138 80 76 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2117 2149 5841 5873 2117 Size: 32 3692 32 2125 Fragments arranged by size: 3692 2125 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5881 XbaI T'CTAG_A Cuts at: 3217 3217 Size: 5881 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5881 XmaI C'CCGG_G Cuts at: 1870 3206 1870 Size: 1336 4545 XmnI GAAnn'nnTTC Cuts at: 2193 2940 4010 2193 Size: 747 1070 4064 Fragments arranged by size: 4064 1070 747 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3689 276 Size: 53 83 186 936 2155 2468 Fragments arranged by size: 2468 2155 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI