Restriction map for
pFN21AB0931
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# NO CUT ENZYMES ( 176 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcsI AcyI AfaI AflII AflIII
AgeI AhdI AluI Alw44I AlwNI
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AspI AvaI AviII AvrII BamHI
BbeI BbrPI BbsI BbvCI BcgI
BciVI BclI BcnI BfaI BfrI
BglII BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BseRI BsiEI BsiHKAI BsiWI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspEI BspLU11I BsrBI BsrDI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstUI BstZ17I CelII CfoI
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FauI FbaI FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HinfI HpaI KasI
KpnI KspI MaeI MamI MfeI
MluI MroI MseI MspA1I MunI
MvnI NaeI NarI NciI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI PvuII RsaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TfiI Tru9I Tsp509I Tth111I Van91I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AccI 244 GTAGAC GT^[AC][GT]AC
AlwI 192 GATCC GGATC(4/5)
AocI 59 CCTCAGG CC^T[ACGT]AGG
ApaI 55 GGGCCC GGGCC^C
BalI 207 TGGCCA TGG^CCA
BanI 372 GGTGCC G^G[CT][GA]CC
BanII 55 GGGCCC G[GA]GC[CT]^C
BglI 57 GCCCTCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI 55 GGGCCC G[AGT]GC[ACT]^C
BpmI 175 CTCCAG CTGGAG(16/14)
Bsp1286I 55 GGGCCC G[AGT]GC[ACT]^C
BspHI 413 TCATGA T^CATGA
BspMI 224 ACCTGC ACCTGC(4/8)
BsrFI 422 ACCGGC [GA]^CCGG[CT]
BsrI 376 CCAGT ACTGG(1/-1)
BstAPI 262 GCAGGCTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 356 CCATATTTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 191 AGATCC [GA]^GATC[CT]
Bsu36I 59 CCTCAGG CC^T[ACGT]AGG
Cac8I 262 GCAGGC GC[ACGT]^[ACGT]GC
Cfr10I 422 ACCGGC [GA]^CCGG[CT]
DsaI 392 CCATGG C^C[GA][CT]GG
EaeI 207 TGGCCA [CT]^GGCC[GA]
Eco81I 59 CCTCAGG CC^T[ACGT]AGG
EcoT14I 392 CCATGG C^C[AT][AT]GG
HapII 423 CCGG C^CGG
HpaII 423 CCGG C^CGG
MaeII 128 ACGT A^CGT
MflI 191 AGATCC [GA]^GATC[CT]
MluNI 207 TGGCCA TGG^CCA
MscI 207 TGGCCA TGG^CCA
MspI 423 CCGG C^CGG
NcoI 392 CCATGG C^CATGG
PpuMI 338 GGGACCC [GA]G^G[AT]CC[CT]
RcaI 413 TCATGA T^CATGA
StyI 392 CCATGG C^C[AT][AT]GG
TaqI 327 TCGA T^CGA
Tsp45I 25 GTCAC ^GT[GC]AC
TthHB8I 327 TCGA T^CGA
XhoII 191 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 13 enzymes) #
###################################
AvaII 313 GGACC G^G[AT]CC
339 GGACC
BsiYI 161 CCCTGGATGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
387 CCTCACCATGG
BslI 161 CCCTGGATGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
387 CCTCACCATGG
BsmAI 106 GTCTC GTCTC(1/5)
276 GTCTC
BsmFI 88 GGGAC GGGAC(10/14)
338 GGGAC
BstF5I 165 GGATG GGATG(2/0)
237 GGATG
EarI 8 GAAGAG CTCTTC(1/4)
34 GAAGAG
FokI 165 GGATG GGATG(9/13)
237 GGATG
Ksp632I 8 GAAGAG CTCTTC(1/4)
34 GAAGAG
MaeIII 25 GTCAC ^GT[ACGT]AC
297 GTTAC
MboII 8 GAAGA GAAGA(8/7)
34 GAAGA
NlaIII 393 CATG CATG^
414 CATG
SfcI 242 CTGTAG C^T[GA][CT]AG
317 CTATAG
#########################################
# 3 OR MORE CUT ENZYMES ( 29 enzymes) #
#########################################
AciI BbvI BsaJI BsgI BssKI
BstNI Cfr13I DdeI DpnII DraII
EcoO109I EcoRII Fnu4HI HaeIII HphI
ItaI MboI MnlI MslI MvaI
MwoI NdeII NlaIV Sau3AI Sau96I
ScrFI SfaNI TseI TspRI