Restriction map for pFN21AB0930
 (Circular) (Six-base) MAPSORT of: pFN21AB0930.seq  Check: 247  from: 1  to: 5818



pFN21AB0930  5818 bp

 With 182 enzymes: SgfI * 

                        April 15, 2010 09:33  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3628     278 
   Size:         53      83     186     936    2092    2468

  Fragments arranged by size: 

                2468    2092     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    3161    1937 
   Size:       1224    4594
 
Acc65I G'GTAC_C

Cuts at:   3139    3139 
   Size:       5818
 
AclI AA'CG_TT

Cuts at:   1563    3945    4318    1563 
   Size:       2382     373    3063

  Fragments arranged by size: 

                3063    2382     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2017    2110    2359    2753    2980    3881    5265    5533 
   Size:        385      93     249     394     227     901    1384     268

Cuts at:   5533    1632 
   Size:       1917

  Fragments arranged by size: 

                1917    1384     901     394     385     268     249     227
                  93
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5621
 
AflIII A'CryG_T

Cuts at:   1203    4717    5694    1203 
   Size:       3514     977    1327

  Fragments arranged by size: 

                3514    1327     977
 
AgeI A'CCGG_T

Cuts at:   4649    4649 
   Size:       5818
 
AhdI GACnn_n'nnGTC

Cuts at:   4547    4547 
   Size:       5818
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5818
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5818
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2849    2881    2849 
   Size:         32    5786
 
AlwNI CAG_nnn'CTG

Cuts at:   3561    5133    3561 
   Size:       1572    4246
 
ApaI G_GGCC'C

Cuts at:   2098    2098 
   Size:       5818
 
ApaLI G'TGCA_C

Cuts at:   2594    2846    3875    5031    2594 
   Size:        252    1029    1156    3381

  Fragments arranged by size: 

                3381    1156    1029     252
 
ApoI r'AATT_y

Cuts at:   1493    3127    3353    3505    5614    1493 
   Size:       1634     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697    1634     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    4372     160 
   Size:        872    3340    1606

  Fragments arranged by size: 

                3340    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5818
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2624    3143    1870 
   Size:         73     681     519    4545

  Fragments arranged by size: 

                4545     681     519      73
 
BamHI G'GATC_C

Cuts at:   3148    3515    3148 
   Size:        367    5451
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    3139    4594     618 
   Size:        325     217     627    1352    1455    1842

  Fragments arranged by size: 

                1842    1455    1352     627     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2098    2314    3137    1391 
   Size:        707     216     823    4072

  Fragments arranged by size: 

                4072     823     707     216
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2217     928 
   Size:        971     318    4529

  Fragments arranged by size: 

                4529     971     318
 
BbvCI CC'TCA_GC

Cuts at:   2912    2912 
   Size:       5818
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    4009    4043    1903 
   Size:         34    2072      34    3678

  Fragments arranged by size: 

                3678    2072      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3710    4920    3710 
   Size:       1210    4608
 
BclI T'GATC_A

Cuts at:   1548    2296    1548 
   Size:        748    5070
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    4429     136 
   Size:        107     122      71     631     605    2757    1525

  Fragments arranged by size: 

                2757    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   5813    5813 
   Size:       5818
 
BlpI GC'TnA_GC

Cuts at:   2558    2748    3235    2558 
   Size:        190     487    5141

  Fragments arranged by size: 

                5141     487     190
 
Bme1580I G_kGCm'C

Cuts at:   1790    2098    2598    2624    2850    3879    5035    1790 
   Size:        308     500      26     226    1029    1156    2573

  Fragments arranged by size: 

                2573    1156    1029     500     308     226      26
 
BmgBI CAC'GTC

Cuts at:   1318    2041    2110    1318 
   Size:        723      69    5026

  Fragments arranged by size: 

                5026     723      69
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    3549    4507     442 
   Size:        416      53      83     399      24    2132     958    1753

  Fragments arranged by size: 

                2132    1753     958     416     399      83      53      24
            
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5818
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2400    4478    1239 
   Size:       1161    2078    2579

  Fragments arranged by size: 

                2579    2078    1161
 
Bpu10I CC'TnA_GC

Cuts at:   2912    2912 
   Size:       5818
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2041    3943    4808    5106    2041 
   Size:       1902     865     298    2753

  Fragments arranged by size: 

                2753    1902     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    2215    4481     882 
   Size:        617     716    2266    2219

  Fragments arranged by size: 

                2266    2219     716     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    2329    2544    3609     493 
   Size:        711    1125     215    1065    2702

  Fragments arranged by size: 

                2702    1125    1065     711     215
 
BsaBI GATnn'nnATC

Cuts at:   5812    5812 
   Size:       5818
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    2430    3625    4007    4642 
   Size:         53      83     186     936     897    1195     382     635

Cuts at:   4642     275 
   Size:       1451

  Fragments arranged by size: 

                1451    1195     936     897     635     382     186      83
                  53
 
BsaWI w'CCGG_w

Cuts at:   2643    2895    2979    3006    4251    4649    4923    5070    5481 
   Size:        252      84      27    1245     398     274     147     411

Cuts at:   5481    2643 
   Size:       2980

  Fragments arranged by size: 

                2980    1245     411     398     274     252     147      84
                  27
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    2057    2087    2186    2216    2849 
   Size:         30     165      30     654      30      99      30     633

Cuts at:   2849    2879    1178 
   Size:         30    4117

  Fragments arranged by size: 

                4117     654     633     165      99      30      30      30
                  30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2078    2210    1186 
   Size:        892     132    4794

  Fragments arranged by size: 

                4794     892     132
 
BseYI C'CCAG_C

Cuts at:   2084    5021    5517    2084 
   Size:       2937     496    2385

  Fragments arranged by size: 

                2937    2385     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    2261    2999    1163 
   Size:        573     525     738    3982

  Fragments arranged by size: 

                3982     738     573     525
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2486    2864    3268    4029    5057    1540 
   Size:        275     186     485     378     404     761    1028    2301

  Fragments arranged by size: 

                2301    1028     761     485     404     378     275     186
            
 
BsiHKAI G_wGCw'C

Cuts at:   2463    2598    2775    2850    3879    3964    5035    2463 
   Size:        135     177      75    1029      85    1071    3246

  Fragments arranged by size: 

                3246    1071    1029     177     135      85      75
 
BsmI GAATG_Cn'

Cuts at:   3329    3422    3329 
   Size:         93    5725
 
BsmBI CGTCTCn'nnnn_

Cuts at:   2179    2179 
   Size:       5818
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2098    2314    2463    2598    2624    2775    2850 
   Size:        399     308     216     149     135      26     151      75

Cuts at:   2850    3137    3879    3964    5035    1391 
   Size:        287     742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     742     399     308     287     216     151
                 149     135      85      75      26
 
BspEI T'CCGG_A

Cuts at:   2895    5481    2895 
   Size:       2586    3232
 
BspHI T'CATG_A

Cuts at:   3707    5437    3707 
   Size:       1730    4088
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2667    3173     844 
   Size:       1823     506    3489

  Fragments arranged by size: 

                3489    1823     506
 
BsrBI CCG'CTC

Cuts at:   1394    1823    2860    3705    1394 
   Size:        429    1037     845    3507

  Fragments arranged by size: 

                3507    1037     845     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    4313    4487      65 
   Size:       1183    3065     174    1396

  Fragments arranged by size: 

                3065    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    2571    4462    4649    1700 
   Size:        110     761    1891     187    2869

  Fragments arranged by size: 

                2869    1891     761     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5818
 
BssHII G'CGCG_C

Cuts at:   1925    2512    1925 
   Size:        587    5231
 
BssSI C'ACGA_G

Cuts at:   2785    3878    4890    2785 
   Size:       1093    1012    3713

  Fragments arranged by size: 

                3713    1093    1012
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       5818
 
BstBI TT'CG_AA

Cuts at:   4622    4622 
   Size:       5818
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5818
 
BstXI CCAn_nnnn'nTGG

Cuts at:   2011    2011 
   Size:       5818
 
BstYI r'GATC_y

Cuts at:   1972    3148    3515    3900    3917    5369    5455    5467    5813 
   Size:       1176     367     385      17    1452      86      12     346

Cuts at:   5813    1972 
   Size:       1977

  Fragments arranged by size: 

                1977    1452    1176     385     367     346      86      17
                  12
 
BtgI C'CryG_G

Cuts at:    513    1065    2004    2201    2439     513 
   Size:        552     939     197     238    3892

  Fragments arranged by size: 

                3892     939     552     238     197
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    5079

  Fragments arranged by size: 

                5079     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2137    2244    3334    4128    4148    2137 
   Size:        107    1090     794      20    3807

  Fragments arranged by size: 

                3807    1090     794     107      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5818
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5783
 
DraI TTT'AAA

Cuts at:   3122    3122 
   Size:       5818
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4825     809 
   Size:        507    3509    1802

  Fragments arranged by size: 

                3509    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2043    2255    2483 
   Size:         54    1425      50     275      18     213     212     228

Cuts at:   2483    2632    2861    3043    3265    4154    5783       8 
   Size:        149     229     182     222     889    1629      43

  Fragments arranged by size: 

                1629    1425     889     275     229     228     222     213
                 212     182     149      54      50      43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    2483    2861    3265    1537 
   Size:        275     671     378     404    4090

  Fragments arranged by size: 

                4090     671     404     378     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    3020    3748    1353 
   Size:       1667     728    3423

  Fragments arranged by size: 

                3423    1667     728
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    4405    4779    4925     387 
   Size:       1064     138    2816     374     146    1280

  Fragments arranged by size: 

                2816    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2017    2110    2359    2400    2753    2980    3881 
   Size:        393     385      93     249      41     353     227     901

Cuts at:   3881    4478    5265    5533    1239 
   Size:        597     787     268    1524

  Fragments arranged by size: 

                1524     901     787     597     393     385     353     268
                 249     227      93      41
 
EcoO109I rG'GnC_Cy

Cuts at:   2094    2095    2249    2778    2094 
   Size:          1     154     529    5134

  Fragments arranged by size: 

                5134     529     154       1
 
EcoRI G'AATT_C

Cuts at:   3127    3505    3127 
   Size:        378    5440
 
FspI TGC'GCA

Cuts at:   4324    4324 
   Size:       5818
 
HaeII r_GCGC'y

Cuts at:   1678    2503    2558    2668    4965    1678 
   Size:        825      55     110    2297    2531

  Fragments arranged by size: 

                2531    2297     825     110      55
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2154    2159    2186    2191    4459 
   Size:         32     135      32     582       5      27       5    2268

Cuts at:   4459    4491    4533    4565    1373 
   Size:         32      42      32    2626

  Fragments arranged by size: 

                2626    2268     582     135      42      32      32      32
                  32      27       5       5
 
HincII GTy'rAC

Cuts at:    669    1657    1938    3162    3408     669 
   Size:        988     281    1224     246    3079

  Fragments arranged by size: 

                3079    1224     988     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5818
 
HpaI GTT'AAC

Cuts at:   3408    3408 
   Size:       5818
 
KpnI G_GTAC'C

Cuts at:   3143    3143 
   Size:       5818
 
MfeI C'AATT_G

Cuts at:   3417    3417 
   Size:       5818
 
MluI A'CGCG_T

Cuts at:   5694    5694 
   Size:       5818
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    2847    4932    5116    1305 
   Size:       1542    2085     184    2007

  Fragments arranged by size: 

                2085    2007    1542     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832    2045      10 
   Size:         54    1425     343     213    3783

  Fragments arranged by size: 

                3783    1425     343     213      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2009    3776    4135    4294     518 
   Size:        648     843    1767     359     159    2042

  Fragments arranged by size: 

                2042    1767     843     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2562    2842    2937    3234    3911    5059    5304    1606 
   Size:        956     280      95     297     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148     956     677     297     280     245      95
            
 
NaeI GCC'GGC

Cuts at:   2573    2573 
   Size:       5818
 
NcoI C'CATG_G

Cuts at:    513    1065    2004     513 
   Size:        552     939    4327

  Fragments arranged by size: 

                4327     939     552
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5818
 
NgoMIV G'CCGG_C

Cuts at:   2571    2571 
   Size:       5818
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5818
 
NotI GC'GGCC_GC

Cuts at:   3265    3265 
   Size:       5818
 
NspI r_CATG'y

Cuts at:   1132    1229    3176    4721    1132 
   Size:         97    1947    1545    2229

  Fragments arranged by size: 

                2229    1947    1545      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5818
 
PciI A'CATG_T

Cuts at:   4717    4717 
   Size:       5818
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       5818
 
PmeI GTTT'AAAC

Cuts at:   3122    3122 
   Size:       5818
 
PmlI CAC'GTG

Cuts at:   2329    2544    2329 
   Size:        215    5603
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2513    2545    2849    2881    3887    3919    5400    5432    2513 
   Size:         32     304      32    1006      32    1481      32    2899

  Fragments arranged by size: 

                2899    1481    1006     304      32      32      32      32
            
 
PpuMI rG'GwC_Cy

Cuts at:   2249    2249 
   Size:       5818
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5818
 
PsiI TTA'TAA

Cuts at:   3388    3388 
   Size:       5818
 
PspOMI G'GGCC_C

Cuts at:   2094    2094 
   Size:       5818
 
PstI C_TGCA'G

Cuts at:    830    2278    2664    3170     830 
   Size:       1448     386     506    3478

  Fragments arranged by size: 

                3478    1448     506     386
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5818
 
PvuII CAG'CTG

Cuts at:   2562    2842    2562 
   Size:        280    5538
 
SalI G'TCGA_C

Cuts at:   1936    3160    1936 
   Size:       1224    4594
 
SanDI GG'GwC_CC

Cuts at:   2249    2249 
   Size:       5818
 
SbfI CC_TGCA'GG

Cuts at:   3170    3170 
   Size:       5818
 
ScaI AGT'ACT

Cuts at:   4066    5778    4066 
   Size:       1712    4106
 
SfcI C'TryA_G

Cuts at:    826    1045    2274    2660    3166    4301    4982    5173     826 
   Size:        219    1229     386     506    1135     681     191    1471

  Fragments arranged by size: 

                1471    1229    1135     681     506     386     219     191
            
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5818
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5818
 
SmaI CCC'GGG

Cuts at:   1872    3145    1872 
   Size:       1273    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    2056    3922    4823    5085     820 
   Size:        197     926     113    1866     901     262    1553

  Fragments arranged by size: 

                1866    1553     926     901     262     197     113
 
SnaBI TAC'GTA

Cuts at:    493    3609     493 
   Size:       3116    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5818
 
SphI G_CATG'C

Cuts at:   1132    3176    1132 
   Size:       2044    3774
 
SspI AAT'ATT

Cuts at:      5      52    3742       5 
   Size:         47    3690    2081

  Fragments arranged by size: 

                3690    2081      47
 
StuI AGG'CCT

Cuts at:   1517    2612    2711    1517 
   Size:       1095      99    4624

  Fragments arranged by size: 

                4624    1095      99
 
StyI C'CwwG_G

Cuts at:    513    1065    2004    2607    2901    2988    3257     513 
   Size:        552     939     603     294      87     269    3074

  Fragments arranged by size: 

                3074     939     603     552     294     269      87
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    3845    3862    4015    4200    4645     325 
   Size:       1255    2265      17     153     185     445    1498

  Fragments arranged by size: 

                2265    1498    1255     445     185     153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    4064    5776      96 
   Size:        274      80      33      51    1443    2087    1712     138

  Fragments arranged by size: 

                2087    1712    1443     274     138      80      51      33
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2057    2089    2186    2218    5778    5810    2057 
   Size:         32      97      32    3560      32    2065

  Fragments arranged by size: 

                3560    2065      97      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1316    2171    1316 
   Size:        855    4963
 
XbaI T'CTAG_A

Cuts at:   3154    3154 
   Size:       5818
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5818
 
XmaI C'CCGG_G

Cuts at:   1870    3143    1870 
   Size:       1273    4545
 
XmnI GAAnn'nnTTC

Cuts at:   2372    2696    2807    3029    3059    3947    2372 
   Size:        324     111     222      30     888    4243

  Fragments arranged by size: 

                4243     888     324     222     111      30
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3626     276 
   Size:         53      83     186     936    2092    2468

  Fragments arranged by size: 

                2468    2092     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AseI    AsiSI     AvaI    BamHI     BanI    BanII     BbsI    BbvCI
     BcgI    BciVI     BclI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmrI     BmtI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
    BstYI     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI     DrdI
     EaeI     EagI     EarI     EciI  Eco57MI EcoO109I    EcoRI     FspI
    HaeII    Hin4I   HincII  HindIII     HpaI     KpnI     MfeI     MluI
     MmeI     MscI     MslI   MspA1I     NaeI     NcoI     NdeI   NgoMIV
     NheI     NotI     NspI     PasI     PciI    PflMI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI     PstI     PvuI    PvuII
     SalI    SanDI     SbfI     ScaI     SfcI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SspI     StuI     StyI    TaqII
     TatI     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AscI    AvrII     BaeI     BbeI    BfrBI     BplI
    BsiWI  BstZ17I   Bsu36I   DraIII  EcoICRI    EcoNI    EcoRV     FalI
     FseI    FspAI     KasI     NarI     NruI     NsiI     PacI     PfoI
    PspXI     PsrI    RsrII     SacI    SacII     SapI    SexAI     SfiI
     SfoI     SrfI     SwaI     XcmI
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