(Circular) (Six-base) MAPSORT of: pFN21AB0930.seq Check: 247 from: 1 to: 5818 pFN21AB0930 5818 bp With 182 enzymes: SgfI * April 15, 2010 09:33 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3628 278 Size: 53 83 186 936 2092 2468 Fragments arranged by size: 2468 2092 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3161 1937 Size: 1224 4594 Acc65I G'GTAC_C Cuts at: 3139 3139 Size: 5818 AclI AA'CG_TT Cuts at: 1563 3945 4318 1563 Size: 2382 373 3063 Fragments arranged by size: 3063 2382 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2017 2110 2359 2753 2980 3881 5265 5533 Size: 385 93 249 394 227 901 1384 268 Cuts at: 5533 1632 Size: 1917 Fragments arranged by size: 1917 1384 901 394 385 268 249 227 93 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5621 AflIII A'CryG_T Cuts at: 1203 4717 5694 1203 Size: 3514 977 1327 Fragments arranged by size: 3514 1327 977 AgeI A'CCGG_T Cuts at: 4649 4649 Size: 5818 AhdI GACnn_n'nnGTC Cuts at: 4547 4547 Size: 5818 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5818 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5818 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2849 2881 2849 Size: 32 5786 AlwNI CAG_nnn'CTG Cuts at: 3561 5133 3561 Size: 1572 4246 ApaI G_GGCC'C Cuts at: 2098 2098 Size: 5818 ApaLI G'TGCA_C Cuts at: 2594 2846 3875 5031 2594 Size: 252 1029 1156 3381 Fragments arranged by size: 3381 1156 1029 252 ApoI r'AATT_y Cuts at: 1493 3127 3353 3505 5614 1493 Size: 1634 226 152 2109 1697 Fragments arranged by size: 2109 1697 1634 226 152 AseI AT'TA_AT Cuts at: 160 1032 4372 160 Size: 872 3340 1606 Fragments arranged by size: 3340 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5818 AvaI C'yCGr_G Cuts at: 1870 1943 2624 3143 1870 Size: 73 681 519 4545 Fragments arranged by size: 4545 681 519 73 BamHI G'GATC_C Cuts at: 3148 3515 3148 Size: 367 5451 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3139 4594 618 Size: 325 217 627 1352 1455 1842 Fragments arranged by size: 1842 1455 1352 627 325 217 BanII G_rGCy'C Cuts at: 1391 2098 2314 3137 1391 Size: 707 216 823 4072 Fragments arranged by size: 4072 823 707 216 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2217 928 Size: 971 318 4529 Fragments arranged by size: 4529 971 318 BbvCI CC'TCA_GC Cuts at: 2912 2912 Size: 5818 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4009 4043 1903 Size: 34 2072 34 3678 Fragments arranged by size: 3678 2072 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3710 4920 3710 Size: 1210 4608 BclI T'GATC_A Cuts at: 1548 2296 1548 Size: 748 5070 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4429 136 Size: 107 122 71 631 605 2757 1525 Fragments arranged by size: 2757 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5813 5813 Size: 5818 BlpI GC'TnA_GC Cuts at: 2558 2748 3235 2558 Size: 190 487 5141 Fragments arranged by size: 5141 487 190 Bme1580I G_kGCm'C Cuts at: 1790 2098 2598 2624 2850 3879 5035 1790 Size: 308 500 26 226 1029 1156 2573 Fragments arranged by size: 2573 1156 1029 500 308 226 26 BmgBI CAC'GTC Cuts at: 1318 2041 2110 1318 Size: 723 69 5026 Fragments arranged by size: 5026 723 69 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3549 4507 442 Size: 416 53 83 399 24 2132 958 1753 Fragments arranged by size: 2132 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5818 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2400 4478 1239 Size: 1161 2078 2579 Fragments arranged by size: 2579 2078 1161 Bpu10I CC'TnA_GC Cuts at: 2912 2912 Size: 5818 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2041 3943 4808 5106 2041 Size: 1902 865 298 2753 Fragments arranged by size: 2753 1902 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2215 4481 882 Size: 617 716 2266 2219 Fragments arranged by size: 2266 2219 716 617 BsaAI yAC'GTr Cuts at: 493 1204 2329 2544 3609 493 Size: 711 1125 215 1065 2702 Fragments arranged by size: 2702 1125 1065 711 215 BsaBI GATnn'nnATC Cuts at: 5812 5812 Size: 5818 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2430 3625 4007 4642 Size: 53 83 186 936 897 1195 382 635 Cuts at: 4642 275 Size: 1451 Fragments arranged by size: 1451 1195 936 897 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2643 2895 2979 3006 4251 4649 4923 5070 5481 Size: 252 84 27 1245 398 274 147 411 Cuts at: 5481 2643 Size: 2980 Fragments arranged by size: 2980 1245 411 398 274 252 147 84 27 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2057 2087 2186 2216 2849 Size: 30 165 30 654 30 99 30 633 Cuts at: 2849 2879 1178 Size: 30 4117 Fragments arranged by size: 4117 654 633 165 99 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2078 2210 1186 Size: 892 132 4794 Fragments arranged by size: 4794 892 132 BseYI C'CCAG_C Cuts at: 2084 5021 5517 2084 Size: 2937 496 2385 Fragments arranged by size: 2937 2385 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2261 2999 1163 Size: 573 525 738 3982 Fragments arranged by size: 3982 738 573 525 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2486 2864 3268 4029 5057 1540 Size: 275 186 485 378 404 761 1028 2301 Fragments arranged by size: 2301 1028 761 485 404 378 275 186 BsiHKAI G_wGCw'C Cuts at: 2463 2598 2775 2850 3879 3964 5035 2463 Size: 135 177 75 1029 85 1071 3246 Fragments arranged by size: 3246 1071 1029 177 135 85 75 BsmI GAATG_Cn' Cuts at: 3329 3422 3329 Size: 93 5725 BsmBI CGTCTCn'nnnn_ Cuts at: 2179 2179 Size: 5818 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2098 2314 2463 2598 2624 2775 2850 Size: 399 308 216 149 135 26 151 75 Cuts at: 2850 3137 3879 3964 5035 1391 Size: 287 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 308 287 216 151 149 135 85 75 26 BspEI T'CCGG_A Cuts at: 2895 5481 2895 Size: 2586 3232 BspHI T'CATG_A Cuts at: 3707 5437 3707 Size: 1730 4088 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2667 3173 844 Size: 1823 506 3489 Fragments arranged by size: 3489 1823 506 BsrBI CCG'CTC Cuts at: 1394 1823 2860 3705 1394 Size: 429 1037 845 3507 Fragments arranged by size: 3507 1037 845 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4313 4487 65 Size: 1183 3065 174 1396 Fragments arranged by size: 3065 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2571 4462 4649 1700 Size: 110 761 1891 187 2869 Fragments arranged by size: 2869 1891 761 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5818 BssHII G'CGCG_C Cuts at: 1925 2512 1925 Size: 587 5231 BssSI C'ACGA_G Cuts at: 2785 3878 4890 2785 Size: 1093 1012 3713 Fragments arranged by size: 3713 1093 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5818 BstBI TT'CG_AA Cuts at: 4622 4622 Size: 5818 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5818 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2011 Size: 5818 BstYI r'GATC_y Cuts at: 1972 3148 3515 3900 3917 5369 5455 5467 5813 Size: 1176 367 385 17 1452 86 12 346 Cuts at: 5813 1972 Size: 1977 Fragments arranged by size: 1977 1452 1176 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2201 2439 513 Size: 552 939 197 238 3892 Fragments arranged by size: 3892 939 552 238 197 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5079 Fragments arranged by size: 5079 683 56 BtsI GCAGTG_nn' Cuts at: 2137 2244 3334 4128 4148 2137 Size: 107 1090 794 20 3807 Fragments arranged by size: 3807 1090 794 107 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5818 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5783 DraI TTT'AAA Cuts at: 3122 3122 Size: 5818 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4825 809 Size: 507 3509 1802 Fragments arranged by size: 3509 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2043 2255 2483 Size: 54 1425 50 275 18 213 212 228 Cuts at: 2483 2632 2861 3043 3265 4154 5783 8 Size: 149 229 182 222 889 1629 43 Fragments arranged by size: 1629 1425 889 275 229 228 222 213 212 182 149 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2483 2861 3265 1537 Size: 275 671 378 404 4090 Fragments arranged by size: 4090 671 404 378 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3020 3748 1353 Size: 1667 728 3423 Fragments arranged by size: 3423 1667 728 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4405 4779 4925 387 Size: 1064 138 2816 374 146 1280 Fragments arranged by size: 2816 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2017 2110 2359 2400 2753 2980 3881 Size: 393 385 93 249 41 353 227 901 Cuts at: 3881 4478 5265 5533 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 901 787 597 393 385 353 268 249 227 93 41 EcoO109I rG'GnC_Cy Cuts at: 2094 2095 2249 2778 2094 Size: 1 154 529 5134 Fragments arranged by size: 5134 529 154 1 EcoRI G'AATT_C Cuts at: 3127 3505 3127 Size: 378 5440 FspI TGC'GCA Cuts at: 4324 4324 Size: 5818 HaeII r_GCGC'y Cuts at: 1678 2503 2558 2668 4965 1678 Size: 825 55 110 2297 2531 Fragments arranged by size: 2531 2297 825 110 55 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2154 2159 2186 2191 4459 Size: 32 135 32 582 5 27 5 2268 Cuts at: 4459 4491 4533 4565 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2268 582 135 42 32 32 32 32 27 5 5 HincII GTy'rAC Cuts at: 669 1657 1938 3162 3408 669 Size: 988 281 1224 246 3079 Fragments arranged by size: 3079 1224 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5818 HpaI GTT'AAC Cuts at: 3408 3408 Size: 5818 KpnI G_GTAC'C Cuts at: 3143 3143 Size: 5818 MfeI C'AATT_G Cuts at: 3417 3417 Size: 5818 MluI A'CGCG_T Cuts at: 5694 5694 Size: 5818 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2847 4932 5116 1305 Size: 1542 2085 184 2007 Fragments arranged by size: 2085 2007 1542 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2045 10 Size: 54 1425 343 213 3783 Fragments arranged by size: 3783 1425 343 213 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 3776 4135 4294 518 Size: 648 843 1767 359 159 2042 Fragments arranged by size: 2042 1767 843 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2562 2842 2937 3234 3911 5059 5304 1606 Size: 956 280 95 297 677 1148 245 2120 Fragments arranged by size: 2120 1148 956 677 297 280 245 95 NaeI GCC'GGC Cuts at: 2573 2573 Size: 5818 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4327 Fragments arranged by size: 4327 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5818 NgoMIV G'CCGG_C Cuts at: 2571 2571 Size: 5818 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5818 NotI GC'GGCC_GC Cuts at: 3265 3265 Size: 5818 NspI r_CATG'y Cuts at: 1132 1229 3176 4721 1132 Size: 97 1947 1545 2229 Fragments arranged by size: 2229 1947 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5818 PciI A'CATG_T Cuts at: 4717 4717 Size: 5818 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5818 PmeI GTTT'AAAC Cuts at: 3122 3122 Size: 5818 PmlI CAC'GTG Cuts at: 2329 2544 2329 Size: 215 5603 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2513 2545 2849 2881 3887 3919 5400 5432 2513 Size: 32 304 32 1006 32 1481 32 2899 Fragments arranged by size: 2899 1481 1006 304 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2249 2249 Size: 5818 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5818 PsiI TTA'TAA Cuts at: 3388 3388 Size: 5818 PspOMI G'GGCC_C Cuts at: 2094 2094 Size: 5818 PstI C_TGCA'G Cuts at: 830 2278 2664 3170 830 Size: 1448 386 506 3478 Fragments arranged by size: 3478 1448 506 386 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5818 PvuII CAG'CTG Cuts at: 2562 2842 2562 Size: 280 5538 SalI G'TCGA_C Cuts at: 1936 3160 1936 Size: 1224 4594 SanDI GG'GwC_CC Cuts at: 2249 2249 Size: 5818 SbfI CC_TGCA'GG Cuts at: 3170 3170 Size: 5818 ScaI AGT'ACT Cuts at: 4066 5778 4066 Size: 1712 4106 SfcI C'TryA_G Cuts at: 826 1045 2274 2660 3166 4301 4982 5173 826 Size: 219 1229 386 506 1135 681 191 1471 Fragments arranged by size: 1471 1229 1135 681 506 386 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5818 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5818 SmaI CCC'GGG Cuts at: 1872 3145 1872 Size: 1273 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2056 3922 4823 5085 820 Size: 197 926 113 1866 901 262 1553 Fragments arranged by size: 1866 1553 926 901 262 197 113 SnaBI TAC'GTA Cuts at: 493 3609 493 Size: 3116 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5818 SphI G_CATG'C Cuts at: 1132 3176 1132 Size: 2044 3774 SspI AAT'ATT Cuts at: 5 52 3742 5 Size: 47 3690 2081 Fragments arranged by size: 3690 2081 47 StuI AGG'CCT Cuts at: 1517 2612 2711 1517 Size: 1095 99 4624 Fragments arranged by size: 4624 1095 99 StyI C'CwwG_G Cuts at: 513 1065 2004 2607 2901 2988 3257 513 Size: 552 939 603 294 87 269 3074 Fragments arranged by size: 3074 939 603 552 294 269 87 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3845 3862 4015 4200 4645 325 Size: 1255 2265 17 153 185 445 1498 Fragments arranged by size: 2265 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4064 5776 96 Size: 274 80 33 51 1443 2087 1712 138 Fragments arranged by size: 2087 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2057 2089 2186 2218 5778 5810 2057 Size: 32 97 32 3560 32 2065 Fragments arranged by size: 3560 2065 97 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2171 1316 Size: 855 4963 XbaI T'CTAG_A Cuts at: 3154 3154 Size: 5818 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5818 XmaI C'CCGG_G Cuts at: 1870 3143 1870 Size: 1273 4545 XmnI GAAnn'nnTTC Cuts at: 2372 2696 2807 3029 3059 3947 2372 Size: 324 111 222 30 888 4243 Fragments arranged by size: 4243 888 324 222 111 30 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3626 276 Size: 53 83 186 936 2092 2468 Fragments arranged by size: 2468 2092 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PspXI PsrI RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI XcmI