Restriction map for
pFN21AB0919
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# NO CUT ENZYMES ( 169 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AfaI AflII AflIII
AgeI AhdI Alw44I Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
AvrII BalI BanI BbeI BbrPI
BcgI BciVI BclI BfaI BfrI
BglII BlnI BlpI Bpu1102I BsaBI
BsaHI BsaI BsaWI BseAI BsgI
BsiEI BsiWI BsmAI BsmBI BsmFI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrDI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstZ17I CelII ClaI
CpoI DraI DraIII DrdI EaeI
EagI Eam1105I EarI EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HgaI
Hin1I HincII HindII HindIII HinfI
HpaI KasI KpnI Ksp632I KspI
MaeI MaeIII MamI MfeI MluI
MluNI MroI MscI MseI MslI
MunI NarI NcoI NdeI NheI
NlaIII NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsaI RsrII SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TaqI TfiI Tru9I
Tsp45I Tsp509I Tth111I TthHB8I Van91I
XbaI XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 33 enzymes) #
###################################
AccII 22 CGCG CG^CG
AocI 347 CCTCAGG CC^T[ACGT]AGG
AspHI 262 GAGCTC G[AT]GC[AT]^C
AvaI 171 CCCGGG C^[CT]CG[GA]G
AvaII 285 GGACC G^G[AT]CC
BamHI 99 GGATCC G^GATCC
BbsI 127 GAAGAC GAAGAC(2/6)
BbvCI 246 GCTGAGG CCTCAGC(-5/-2)
BglI 202 GCCTACGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 127 GAAGAC GAAGAC(2/6)
BsaAI 205 TACGTG [CT]AC^GT[GA]
BseRI 345 CTCCTC GAGGAG(10/8)
BsiHKAI 262 GAGCTC G[AT]GC[AT]^C
BsoBI 171 CCCGGG C^[CT]CG[GA]G
BsrBI 292 GAGCGG CCGCTC(-3/-3)
BstUI 22 CGCG CG^CG
Bsu36I 347 CCTCAGG CC^T[ACGT]AGG
DsaI 53 CCACGG C^C[GA][CT]GG
Eco81I 347 CCTCAGG CC^T[ACGT]AGG
EcoT14I 8 CCAAGG C^C[AT][AT]GG
HaeII 45 AGCGCC [GA]GCGC^[CT]
HphI 110 TCACC GGTGA(8/7)
MboII 127 GAAGA GAAGA(8/7)
MspA1I 220 CAGCGG C[AC]G^C[GT]G
MvnI 22 CGCG CG^CG
PpuMI 284 AGGACCT [GA]G^G[AT]CC[CT]
SacI 262 GAGCTC GAGCT^C
SfaNI 160 GATGC GCATC(5/9)
SmaI 171 CCCGGG CCC^GGG
StyI 8 CCAAGG C^C[AT][AT]GG
TspRI 104 CAGTG CA[GC]TG[ACGT][ACGT]^
XmaCI 171 CCCGGG C^CCGGG
XmaI 171 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AluI 182 AGCT AG^CT
263 AGCT
AlwNI 64 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG
220 CAGCGGCTG
BanII 3 GGGCTC G[GA]GC[CT]^C
262 GAGCTC
BmyI 3 GGGCTC G[AGT]GC[ACT]^C
262 GAGCTC
BpmI 70 CTGGAG CTGGAG(16/14)
289 CTGGAG
Bsp1286I 3 GGGCTC G[AGT]GC[ACT]^C
262 GAGCTC
BsrI 103 CCAGT ACTGG(1/-1)
136 CCAGT
BstYI 65 AGATCC [GA]^GATC[CT]
99 GGATCC
DdeI 247 CTGAG C^T[ACGT]AG
348 CTCAG
DpnII 66 GATC ^GATC
100 GATC
DraII 167 AGGCCCC [GA]G^G[ACGT]CC[CT]
284 AGGACCT
EcoO109I 167 AGGCCCC [GA]G^G[ACGT]CC[CT]
284 AGGACCT
MaeII 206 ACGT A^CGT
242 ACGT
MboI 66 GATC ^GATC
100 GATC
MflI 65 AGATCC [GA]^GATC[CT]
99 GGATCC
NaeI 90 GCCGGC GCC^GGC
277 GCCGGC
NdeII 66 GATC ^GATC
100 GATC
NgoMIV 90 GCCGGC G^CCGGC
277 GCCGGC
PstI 226 CTGCAG CTGCA^G
268 CTGCAG
Sau3AI 66 GATC ^GATC
100 GATC
SfcI 226 CTGCAG C^T[GA][CT]AG
268 CTGCAG
XhoII 65 AGATCC [GA]^GATC[CT]
99 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 34 enzymes) #
#########################################
AciI AlwI BbvI BcnI BsaJI
BsiYI BslI BsrFI BssKI BstF5I
BstNI Cac8I CfoI Cfr10I Cfr13I
EcoRII FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HpaII ItaI
MnlI MspI MvaI MwoI NciI
NlaIV Sau96I ScrFI TseI