Restriction map for
pFN21AB6624
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# NO CUT ENZYMES ( 132 enzymes) #
##################################
AatII Acc65I AclI AcyI AflII
AflIII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspI
AvaI AviII BalI BanI BbeI
BbrPI BcgI BciVI BclI BfrI
BglI BsaAI BsaBI BsaHI BsaI
BseRI BsiWI BsoBI Bsp1407I BspDI
BspLU11I BsrBI BsrFI BsrGI BssHII
BssSI BstBI BstEII BstPI BstXI
Bsu36I CfoI Cfr10I ClaI CpoI
DraI DraIII EaeI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV FbaI
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HindIII HpaI KasI
KpnI Ksp632I MaeII MamI MfeI
MluI MluNI MscI MunI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RsrII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspBI SwaI
Tth111I Van91I XbaI XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AccII 69 CGCG CG^CG
AccIII 1150 TCCGGA T^CCGGA
AcsI 178 AAATTC [GA]^AATT[CT]
ApoI 178 AAATTC [GA]^AATT[CT]
Asp700I 174 GAACAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII 990 CCTAGG C^CTAGG
BbvCI 690 GCTGAGG CCTCAGC(-5/-2)
BcnI 86 CCGGG CC^[GC]GG
BlnI 990 CCTAGG C^CTAGG
BlpI 951 GCTCAGC GC^T[ACGT]AGC
BpmI 601 CTGGAG CTGGAG(16/14)
Bpu1102I 951 GCTCAGC GC^T[ACGT]AGC
BseAI 1150 TCCGGA T^CCGGA
BsiEI 94 CGGTCG CG[GA][CT]^CG
BsmBI 185 GAGACG CGTCTC(1/5)
BsmI 850 GCATTC GAATGC(1/-1)
BspEI 1150 TCCGGA T^CCGGA
Bst1107I 1291 GTATAC GTA^TAC
BstUI 69 CGCG CG^CG
BstZ17I 1291 GTATAC GTA^TAC
CelII 951 GCTCAGC GC^T[ACGT]AGC
DrdI 154 GACAAGGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 68 CCGCGG C^C[GA][CT]GG
EcoT22I 291 ATGCAT ATGCA^T
FauI 630 GCGGG CCCGC(4/6)
HincII 1075 GTTGAC GT[CT]^[GA]AC
HindII 1075 GTTGAC GT[CT]^[GA]AC
KspI 68 CCGCGG CCGC^GG
MroI 1150 TCCGGA T^CCGGA
MvnI 69 CGCG CG^CG
NciI 86 CCGGG CC^[GC]GG
NsiI 291 ATGCAT ATGCA^T
PpuMI 387 GGGTCCC [GA]G^G[AT]CC[CT]
PshAI 312 GACTGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 62 CAGCTG CAG^CTG
SacII 68 CCGCGG CCGC^GG
SfaNI 722 GCATC GCATC(5/9)
SphI 589 GCATGC GCATG^C
SspI 958 AATATT AAT^ATT
StuI 694 AGGCCT AGG^CCT
XmnI 174 GAACAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AccI 367 GTAGAC GT^[AC][GT]AC
1291 GTATAC
AspHI 805 GAGCTC G[AT]GC[AT]^C
949 GAGCTC
AvaII 388 GGTCC G^G[AT]CC
795 GGACC
BamHI 252 GGATCC G^GATCC
1102 GGATCC
BanII 805 GAGCTC G[GA]GC[CT]^C
949 GAGCTC
BbsI 675 GAAGAC GAAGAC(2/6)
1204 GAAGAC
BglII 512 AGATCT A^GATCT
1145 AGATCT
BpuAI 675 GAAGAC GAAGAC(2/6)
1204 GAAGAC
BsaWI 332 ACCGGA [AT]^CCGG[AT]
1150 TCCGGA
BsgI 32 GTGCAG GTGCAG(16/14)
45 GTGCAG
BsiHKAI 805 GAGCTC G[AT]GC[AT]^C
949 GAGCTC
BsmFI 632 GGGAC GGGAC(10/14)
389 GTCCC
BspHI 651 TCATGA T^CATGA
1176 TCATGA
BspMI 403 ACCTGC ACCTGC(4/8)
785 ACCTGC
BstAPI 407 GCAAGCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1123 GCAGATTGTGC
BstNI 214 CCAGG CC^[AT]GG
600 CCTGG
Cac8I 407 GCAAGC GC[ACGT]^[ACGT]GC
589 GCATGC
DraII 387 GGGTCCC [GA]G^G[ACGT]CC[CT]
644 AGGCCCT
EcoO109I 387 GGGTCCC [GA]G^G[ACGT]CC[CT]
644 AGGCCCT
EcoRII 214 CCAGG ^CC[AT]GG
600 CCTGG
EcoT14I 845 CCAAGG C^C[AT][AT]GG
990 CCTAGG
HaeIII 645 GGCC GG^CC
695 GGCC
MslI 652 CATGAAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1048 CATGGCAGTG
MspA1I 62 CAGCTG C[AC]G^C[GT]G
68 CCGCGG
MvaI 214 CCAGG CC^[AT]GG
600 CCTGG
NspI 493 GCATGT [GA]CATG^[CT]
589 GCATGC
PleI 742 GACTC GAGTC(4/5)
210 GAGTC
PstI 80 CTGCAG CTGCA^G
314 CTGCAG
RcaI 651 TCATGA T^CATGA
1176 TCATGA
SacI 805 GAGCTC GAGCT^C
949 GAGCTC
SmlI 4 CTCAAG C^T[CT][GA]AG
299 CTCAAG
StyI 845 CCAAGG C^C[AT][AT]GG
990 CCTAGG
TaqI 227 TCGA T^CGA
713 TCGA
TfiI 304 GATTC G^A[AT]TC
710 GATTC
TthHB8I 227 TCGA T^CGA
713 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 50 enzymes) #
#########################################
AciI AfaI AluI AlwI BbvI
BfaI BmyI BsaJI BsiYI BslI
BsmAI Bsp1286I BsrDI BsrI BssKI
BstF5I BstYI Cfr13I DdeI DpnII
Fnu4HI FokI HapII HinfI HpaII
HphI ItaI MaeI MaeIII MboI
MboII MflI MnlI MseI MspI
MwoI NdeII NlaIII NlaIV RsaI
Sau3AI Sau96I ScrFI SfcI Tru9I
TseI Tsp45I Tsp509I TspRI XhoII