Restriction map for
pFN21AB6624
(Circular) (Six-base) MAPSORT of: pFN21AB6624.seq Check: 1377 from: 1 to: 6004
pFN21AB6624 6004 bp
With 182 enzymes: SgfI *
April 15, 2010 09:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2417 2417
Size: 6004
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3814 278
Size: 53 83 186 936 2278 2468
Fragments arranged by size:
2468 2278 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2373 3297 3347 1937
Size: 436 924 50 4594
Fragments arranged by size:
4594 924 436 50
Acc65I G'GTAC_C
Cuts at: 3325 3325
Size: 6004
AclI AA'CG_TT
Cuts at: 1563 4131 4504 1563
Size: 2568 373 3063
Fragments arranged by size:
3063 2568 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2538 4067 5451 5719 1632
Size: 906 1529 1384 268 1917
Fragments arranged by size:
1917 1529 1384 906 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5807
AflIII A'CryG_T
Cuts at: 1203 4903 5880 1203
Size: 3700 977 1327
Fragments arranged by size:
3700 1327 977
AgeI A'CCGG_T
Cuts at: 4835 4835
Size: 6004
AhdI GACnn_n'nnGTC
Cuts at: 4733 4733
Size: 6004
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6004
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6004
AlwNI CAG_nnn'CTG
Cuts at: 3747 5319 3747
Size: 1572 4432
ApaLI G'TGCA_C
Cuts at: 4061 5217 4061
Size: 1156 4848
ApoI r'AATT_y
Cuts at: 1493 2183 3313 3539 3691 5800 1493
Size: 690 1130 226 152 2109 1697
Fragments arranged by size:
2109 1697 1130 690 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4558 160
Size: 872 3526 1606
Fragments arranged by size:
3526 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6004
AvaI C'yCGr_G
Cuts at: 1870 1943 3329 1870
Size: 73 1386 4545
Fragments arranged by size:
4545 1386 73
AvrII C'CTAG_G
Cuts at: 2995 2995
Size: 6004
BamHI G'GATC_C
Cuts at: 2257 3107 3334 3701 2257
Size: 850 227 367 4560
Fragments arranged by size:
4560 850 367 227
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3325 4780 618
Size: 325 217 627 1538 1455 1842
Fragments arranged by size:
1842 1538 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2814 2958 3323 1391
Size: 1423 144 365 4072
Fragments arranged by size:
4072 1423 365 144
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2687 3216 928
Size: 971 788 529 3716
Fragments arranged by size:
3716 971 788 529
BbvCI CC'TCA_GC
Cuts at: 2696 2696
Size: 6004
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4195 4229 1903
Size: 34 50 34 2174 34 3678
Fragments arranged by size:
3678 2174 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3896 5106 3896
Size: 1210 4794
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6004
BfrBI ATG'CAT
Cuts at: 2298 2298
Size: 6004
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4615 136
Size: 107 122 71 631 605 2943 1525
Fragments arranged by size:
2943 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2517 3150 5999 2517
Size: 633 2849 2522
Fragments arranged by size:
2849 2522 633
BlpI GC'TnA_GC
Cuts at: 2957 3421 2957
Size: 464 5540
Bme1580I G_kGCm'C
Cuts at: 1790 2423 4065 5221 1790
Size: 633 1642 1156 2573
Fragments arranged by size:
2573 1642 1156 633
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6004
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2417 3735 4693
Size: 416 53 83 399 24 1000 1318 958
Cuts at: 4693 442
Size: 1753
Fragments arranged by size:
1753 1318 1000 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6004
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2627 4664 1239
Size: 1388 2037 2579
Fragments arranged by size:
2579 2037 1388
Bpu10I CC'TnA_GC
Cuts at: 2253 2696 2253
Size: 443 5561
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1994 2289 4129 4994 5292 1994
Size: 295 1840 865 298 2706
Fragments arranged by size:
2706 1840 865 298 295
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4667 882
Size: 617 3168 2219
Fragments arranged by size:
3168 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3795 493
Size: 711 2591 2702
Fragments arranged by size:
2702 2591 711
BsaBI GATnn'nnATC
Cuts at: 5998 5998
Size: 6004
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3811 4193 4828 275
Size: 53 83 186 936 2278 382 635 1451
Fragments arranged by size:
2278 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2337 3155 4437 4835 5109 5256 5667 2337
Size: 818 1282 398 274 147 411 2674
Fragments arranged by size:
2674 1282 818 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5779
Fragments arranged by size:
5779 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6004
BseYI C'CCAG_C
Cuts at: 2435 5207 5703 2435
Size: 2772 496 2736
Fragments arranged by size:
2772 2736 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2058 2071 1163
Size: 573 322 13 5096
Fragments arranged by size:
5096 573 322 13
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2102 3454 4215 5243 1540
Size: 275 186 101 1352 761 1028 2301
Fragments arranged by size:
2301 1352 1028 761 275 186 101
BsiHKAI G_wGCw'C
Cuts at: 2814 2958 4065 4150 5221 2814
Size: 144 1107 85 1071 3597
Fragments arranged by size:
3597 1107 1071 144 85
BsmI GAATG_Cn'
Cuts at: 2855 3515 3608 2855
Size: 660 93 5251
Fragments arranged by size:
5251 660 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2184 2184
Size: 6004
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2423 2814 2958 3323 4065 4150 5221
Size: 399 633 391 144 365 742 85 1071
Cuts at: 5221 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 633 399 391 365 144
85
BspEI T'CCGG_A
Cuts at: 3155 5667 3155
Size: 2512 3492
BspHI T'CATG_A
Cuts at: 2656 3181 3893 5623 2656
Size: 525 712 1730 3037
Fragments arranged by size:
3037 1730 712 525
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2417 2799 3359 844
Size: 1573 382 560 3489
Fragments arranged by size:
3489 1573 560 382
BsrBI CCG'CTC
Cuts at: 1394 1823 3891 1394
Size: 429 2068 3507
Fragments arranged by size:
3507 2068 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2298 2539 2690 4499 4673 65
Size: 1183 1050 241 151 1809 174 1396
Fragments arranged by size:
1809 1396 1183 1050 241 174 151
BsrFI r'CCGG_y
Cuts at: 1700 1810 4648 4835 1700
Size: 110 2838 187 2869
Fragments arranged by size:
2869 2838 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6004
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6004
BssSI C'ACGA_G
Cuts at: 4064 5076 4064
Size: 1012 4992
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2418 3134 794
Size: 1624 716 3664
Fragments arranged by size:
3664 1624 716
BstBI TT'CG_AA
Cuts at: 4808 4808
Size: 6004
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6004
BstYI r'GATC_y
Cuts at: 1972 2245 2257 2517 3107 3150 3334 3701 4086
Size: 273 12 260 590 43 184 367 385
Cuts at: 4086 4103 5555 5641 5653 5999 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 590 385 367 346 273 260
184 86 43 17 12 12
BstZ17I GTA'TAC
Cuts at: 3298 3298
Size: 6004
BtgI C'CryG_G
Cuts at: 513 1065 2073 513
Size: 552 1008 4444
Fragments arranged by size:
4444 1008 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5265
Fragments arranged by size:
5265 683 56
BtsI GCAGTG_nn'
Cuts at: 2390 2423 2605 3064 3520 4314 4334 2390
Size: 33 182 459 456 794 20 4060
Fragments arranged by size:
4060 794 459 456 182 33 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6004
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5969
DraI TTT'AAA
Cuts at: 3308 3308
Size: 6004
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2165 5011 809
Size: 507 849 2846 1802
Fragments arranged by size:
2846 1802 849 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3451 4340 5969
Size: 54 1425 50 275 18 1621 889 1629
Cuts at: 5969 8
Size: 43
Fragments arranged by size:
1629 1621 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3451 1537
Size: 275 1639 4090
Fragments arranged by size:
4090 1639 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3934 1353
Size: 2581 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4591 4965 5111 387
Size: 1064 138 3002 374 146 1280
Fragments arranged by size:
3002 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2812 2956 2812
Size: 144 5860
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2538 2627 4067 4664 5451 5719 1239
Size: 393 906 89 1440 597 787 268 1524
Fragments arranged by size:
1524 1440 906 787 597 393 268 89
EcoO109I rG'GnC_Cy
Cuts at: 2393 2650 2393
Size: 257 5747
EcoRI G'AATT_C
Cuts at: 3313 3691 3313
Size: 378 5626
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2875 2875
Size: 6004
FspI TGC'GCA
Cuts at: 4510 4510
Size: 6004
HaeII r_GCGC'y
Cuts at: 1678 5151 1678
Size: 3473 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2991 3023 4645 4677 4719
Size: 32 135 32 1419 32 1622 32 42
Cuts at: 4719 4751 1373
Size: 32 2626
Fragments arranged by size:
2626 1622 1419 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3082 3348 3594 669
Size: 988 281 1144 266 246 3079
Fragments arranged by size:
3079 1144 988 281 266 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6004
HpaI GTT'AAC
Cuts at: 3594 3594
Size: 6004
KpnI G_GTAC'C
Cuts at: 3329 3329
Size: 6004
MfeI C'AATT_G
Cuts at: 3603 3603
Size: 6004
MluI A'CGCG_T
Cuts at: 5880 5880
Size: 6004
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2082 2149 3043 5118 5302 1305
Size: 777 67 894 2075 184 2007
Fragments arranged by size:
2075 2007 894 777 184 67
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4182
Fragments arranged by size:
4182 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2661 3057 3962 4321 4480 518
Size: 648 1495 396 905 359 159 2042
Fragments arranged by size:
2042 1495 905 648 396 359 159
MspA1I CmG'CkG
Cuts at: 1606 2069 2075 3420 4097 5245 5490 1606
Size: 463 6 1345 677 1148 245 2120
Fragments arranged by size:
2120 1345 1148 677 463 245 6
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5452
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6004
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6004
NotI GC'GGCC_GC
Cuts at: 3451 3451
Size: 6004
NsiI A_TGCA'T
Cuts at: 2300 2300
Size: 6004
NspI r_CATG'y
Cuts at: 1132 1229 2502 2598 3362 4907 1132
Size: 97 1273 96 764 1545 2229
Fragments arranged by size:
2229 1545 1273 764 97 96
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6004
PciI A'CATG_T
Cuts at: 4903 4903
Size: 6004
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6004
PfoI T'CCnGG_A
Cuts at: 2218 2218
Size: 6004
PmeI GTTT'AAAC
Cuts at: 3308 3308
Size: 6004
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4073 4105 5586 5618 4073
Size: 32 1481 32 4459
Fragments arranged by size:
4459 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2393 2393
Size: 6004
PshAI GACnn'nnGTC
Cuts at: 1613 2321 1613
Size: 708 5296
PsiI TTA'TAA
Cuts at: 3574 3574
Size: 6004
PstI C_TGCA'G
Cuts at: 830 2089 2323 3356 830
Size: 1259 234 1033 3478
Fragments arranged by size:
3478 1259 1033 234
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6004
PvuII CAG'CTG
Cuts at: 2069 2069
Size: 6004
SacI G_AGCT'C
Cuts at: 2814 2958 2814
Size: 144 5860
SacII CC_GC'GG
Cuts at: 2076 2076
Size: 6004
SalI G'TCGA_C
Cuts at: 1936 3346 1936
Size: 1410 4594
SanDI GG'GwC_CC
Cuts at: 2393 2393
Size: 6004
SbfI CC_TGCA'GG
Cuts at: 3356 3356
Size: 6004
ScaI AGT'ACT
Cuts at: 4252 5964 4252
Size: 1712 4292
SfcI C'TryA_G
Cuts at: 826 1045 2085 2319 3138 3352 4487 5168 5359
Size: 219 1040 234 819 214 1135 681 191
Cuts at: 5359 826
Size: 1471
Fragments arranged by size:
1471 1135 1040 819 681 234 219 214
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6004
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6004
SmaI CCC'GGG
Cuts at: 1872 3331 1872
Size: 1459 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2009 2304 4108 5009 5271 820
Size: 197 926 66 295 1804 901 262 1553
Fragments arranged by size:
1804 1553 926 901 295 262 197 66
SnaBI TAC'GTA
Cuts at: 493 3795 493
Size: 3302 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6004
SphI G_CATG'C
Cuts at: 1132 2598 3362 1132
Size: 1466 764 3774
Fragments arranged by size:
3774 1466 764
SspI AAT'ATT
Cuts at: 5 52 2965 3928 5
Size: 47 2913 963 2081
Fragments arranged by size:
2913 2081 963 47
StuI AGG'CCT
Cuts at: 1517 2701 1517
Size: 1184 4820
StyI C'CwwG_G
Cuts at: 513 1065 2850 2995 3443 513
Size: 552 1785 145 448 3074
Fragments arranged by size:
3074 1785 552 448 145
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4031 4048 4201 4386 4831 325
Size: 1255 2451 17 153 185 445 1498
Fragments arranged by size:
2451 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2570 4250 5962
Size: 274 80 33 51 1443 593 1680 1712
Cuts at: 5962 96
Size: 138
Fragments arranged by size:
1712 1680 1443 593 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2200 2232 5964 5996 2200
Size: 32 3732 32 2208
Fragments arranged by size:
3732 2208 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6004
XbaI T'CTAG_A
Cuts at: 3340 3340
Size: 6004
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6004
XmaI C'CCGG_G
Cuts at: 1870 3329 1870
Size: 1459 4545
XmnI GAAnn'nnTTC
Cuts at: 2183 4133 2183
Size: 1950 4054
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3812 276
Size: 53 83 186 936 2278 2468
Fragments arranged by size:
2468 2278 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SacI SacII SalI SanDI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI BaeI BbeI BplI BsiWI
BstXI Bsu36I DraIII EcoNI EcoRV FseI FspAI KasI
NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI
PsrI RsrII SapI SexAI SfiI SfoI SrfI SwaI
XcmI