Restriction map for
pFN21AE3903
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# NO CUT ENZYMES ( 152 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcsI AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaLI
ApoI AscI AseI AsnI Asp718I
AspEI AspHI AspI AviII AvrII
BalI BamHI BbrPI BbsI BbvCI
BciVI BcnI BglII BlnI BlpI
BpmI Bpu1102I BpuAI BsaAI BsaI
BsaWI BseAI BsiHKAI BsiWI BsmBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BsrFI BsrGI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstYI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraI
DraIII DrdI EaeI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV FauI
FseI FspI HapII HgaI HindIII
HpaI HpaII KpnI Ksp632I KspI
MfeI MflI MluI MluNI MroI
MscI MslI MspA1I MspI MunI
MvnI NaeI NciI NgoMIV NheI
NotI NruI NspV PacI PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuII RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tsp45I Tth111I Van91I XbaI XhoII
XmaCI XmaI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AcyI 218 GGCGCC G[GA]^CG[CT]C
AflII 1330 CTTAAG C^TTAAG
AlwNI 434 CAGTCCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 1056 GGGCCC GGGCC^C
AvaI 323 CTCGAG C^[CT]CG[GA]G
AvaII 525 GGTCC G^G[AT]CC
BanII 1056 GGGCCC G[GA]GC[CT]^C
BbeI 218 GGCGCC GGCGC^C
BcgI 1299 CGAGTTCCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 414 TGATCA T^GATCA
BfaI 653 CTAG C^TAG
BfrI 1330 CTTAAG C^TTAAG
BglI 939 GCCTCGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI 1056 GGGCCC G[AGT]GC[ACT]^C
BsaBI 965 GATACCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 218 GGCGCC G[GA]^CG[CT]C
BsiEI 754 CGATCG CG[GA][CT]^CG
BsoBI 323 CTCGAG C^[CT]CG[GA]G
Bsp1286I 1056 GGGCCC G[AGT]GC[ACT]^C
DsaI 106 CCATGG C^C[GA][CT]GG
EcoT14I 106 CCATGG C^C[AT][AT]GG
EcoT22I 1245 ATGCAT ATGCA^T
FbaI 414 TGATCA T^GATCA
Hin1I 218 GGCGCC G[GA]^CG[CT]C
KasI 218 GGCGCC G^GCGCC
MaeI 653 CTAG C^TAG
MaeII 957 ACGT A^CGT
MamI 965 GATACCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 218 GGCGCC GG^CGCC
NcoI 106 CCATGG C^CATGG
NsiI 1245 ATGCAT ATGCA^T
PaeR7I 323 CTCGAG C^TCGAG
PpuMI 524 AGGTCCC [GA]G^G[AT]CC[CT]
PstI 1049 CTGCAG CTGCA^G
PvuI 754 CGATCG CGAT^CG
SfoI 218 GGCGCC GGC^GCC
StyI 106 CCATGG C^C[AT][AT]GG
XcmI 97 CCAATGCGACCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 323 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 22 enzymes) #
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Asp700I 799 GAACATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1180 GAACAACTTC
BanI 218 GGCGCC G^G[CT][GA]CC
465 GGCACC
BsgI 188 CTGCAC GTGCAG(16/14)
486 CTGCAC
BsmAI 36 GAGAC GTCTC(1/5)
246 GTCTC
BsmI 390 GAATGC GAATGC(1/-1)
634 GCATTC
BssSI 1297 CACGAG CACGAG(-5/-1)
1154 CTCGTG
CfoI 42 GCGC GCG^C
219 GCGC
DraII 524 AGGTCCC [GA]G^G[ACGT]CC[CT]
1112 AGGCCCT
EcoO109I 524 AGGTCCC [GA]G^G[ACGT]CC[CT]
1112 AGGCCCT
HaeII 41 AGCGCC [GA]GCGC^[CT]
218 GGCGCC
HhaI 42 GCGC GCG^C
219 GCGC
HinP1I 42 GCGC G^CGC
219 GCGC
HincII 403 GTTGAC GT[CT]^[GA]AC
1318 GTTGAC
HindII 403 GTTGAC GT[CT]^[GA]AC
1318 GTTGAC
MaeIII 454 GTAAC ^GT[ACGT]AC
1292 GTAAC
NdeI 626 CATATG CA^TATG
1019 CATATG
NspI 598 GCATGT [GA]CATG^[CT]
1247 GCATGT
PleI 1261 GAGTC GAGTC(4/5)
1280 GAGTC
SfcI 570 CTGTAG C^T[GA][CT]AG
1049 CTGCAG
SmlI 323 CTCGAG C^T[CT][GA]AG
1330 CTTAAG
TfiI 477 GATTC G^A[AT]TC
1387 GATTC
XmnI 799 GAACATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1180 GAACAACTTC
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# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AfaI AluI AlwI BbvI BsaJI
BseRI BsiYI BslI BsmFI BspMI
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DpnII EcoRII Fnu4HI
FokI HaeIII HinfI HphI ItaI
MboI MboII MnlI MseI MvaI
MwoI NdeII NlaIII NlaIV RsaI
Sau3AI Sau96I ScrFI SfaNI TaqI
Tru9I TseI Tsp509I TspRI TthHB8I