(Circular) (Six-base) MAPSORT of: pFN21AE3903.seq Check: 5423 from: 1 to: 6100 pFN21AE3903 6100 bp With 182 enzymes: SgfI * March 28, 2012 11:41 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2200 3061 2200 Size: 861 5239 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3910 278 Size: 53 83 186 936 2374 2468 Fragments arranged by size: 2468 2374 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3443 1937 Size: 1506 4594 Acc65I G'GTAC_C Cuts at: 3421 3421 Size: 6100 AclI AA'CG_TT Cuts at: 1563 4227 4600 1563 Size: 2664 373 3063 Fragments arranged by size: 3063 2664 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2127 2582 3360 4163 5547 5815 1632 Size: 495 455 778 803 1384 268 1917 Fragments arranged by size: 1917 1384 803 778 495 455 268 AflII C'TTAA_G Cuts at: 820 1017 3335 820 Size: 197 2318 3585 Fragments arranged by size: 3585 2318 197 AflIII A'CryG_T Cuts at: 1203 4999 5976 1203 Size: 3796 977 1327 Fragments arranged by size: 3796 1327 977 AgeI A'CCGG_T Cuts at: 4931 4931 Size: 6100 AhdI GACnn_n'nnGTC Cuts at: 4829 4829 Size: 6100 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6100 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2526 1184 Size: 1342 4758 AlwNI CAG_nnn'CTG Cuts at: 2444 3843 5415 2444 Size: 1399 1572 3129 Fragments arranged by size: 3129 1572 1399 ApaI G_GGCC'C Cuts at: 3065 3065 Size: 6100 ApaLI G'TGCA_C Cuts at: 4157 5313 4157 Size: 1156 4944 ApoI r'AATT_y Cuts at: 1493 3409 3635 3787 5896 1493 Size: 1916 226 152 2109 1697 Fragments arranged by size: 2109 1916 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4654 160 Size: 872 3622 1606 Fragments arranged by size: 3622 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6100 AvaI C'yCGr_G Cuts at: 1870 1943 2328 3425 1870 Size: 73 385 1097 4545 Fragments arranged by size: 4545 1097 385 73 BamHI G'GATC_C Cuts at: 3430 3797 3430 Size: 367 5733 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2223 2470 3421 4876 618 Size: 325 217 627 436 247 951 1455 1842 Fragments arranged by size: 1842 1455 951 627 436 325 247 217 BanII G_rGCy'C Cuts at: 1391 3065 3419 1391 Size: 1674 354 4072 Fragments arranged by size: 4072 1674 354 BbeI G_GCGC'C Cuts at: 2227 2227 Size: 6100 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5129 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3293 3327 4291 4325 1903 Size: 34 1356 34 964 34 3678 Fragments arranged by size: 3678 1356 964 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3992 5202 3992 Size: 1210 4890 BclI T'GATC_A Cuts at: 1548 2419 1548 Size: 871 5229 BfrBI ATG'CAT Cuts at: 3252 3252 Size: 6100 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2950 4711 136 Size: 107 122 71 631 605 1278 1761 1525 Fragments arranged by size: 1761 1525 1278 631 605 122 107 71 BglII A'GATC_T Cuts at: 6095 6095 Size: 6100 BlpI GC'TnA_GC Cuts at: 3517 3517 Size: 6100 Bme1580I G_kGCm'C Cuts at: 1790 3065 4161 5317 1790 Size: 1275 1096 1156 2573 Fragments arranged by size: 2573 1275 1156 1096 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6100 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3152 3831 4789 Size: 416 53 83 399 24 1735 679 958 Cuts at: 4789 442 Size: 1753 Fragments arranged by size: 1753 1735 958 679 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6100 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4760 1239 Size: 3521 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4225 5090 5388 4225 Size: 865 298 4937 Fragments arranged by size: 4937 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4763 882 Size: 617 3264 2219 Fragments arranged by size: 3264 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3891 493 Size: 711 2687 2702 Fragments arranged by size: 2702 2687 711 BsaBI GATnn'nnATC Cuts at: 2974 6094 2974 Size: 3120 2980 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2224 3907 4289 4924 Size: 53 83 186 936 691 1683 382 635 Cuts at: 4924 275 Size: 1451 Fragments arranged by size: 1683 1451 936 691 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4533 4931 5205 5352 5763 4533 Size: 398 274 147 411 4870 Fragments arranged by size: 4870 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2550 2580 2818 2845 2848 Size: 30 165 30 1147 30 238 27 3 Cuts at: 2848 2875 1178 Size: 27 4403 Fragments arranged by size: 4403 1147 238 165 30 30 30 27 27 3 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2336 2577 2673 2697 1186 Size: 1150 241 96 24 4589 Fragments arranged by size: 4589 1150 241 96 24 BseYI C'CCAG_C Cuts at: 2388 2979 3011 5303 5799 2388 Size: 591 32 2292 496 2689 Fragments arranged by size: 2689 2292 591 496 32 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2178 2476 1163 Size: 573 442 298 4787 Fragments arranged by size: 4787 573 442 298 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2762 3550 4311 5339 1540 Size: 275 186 761 788 761 1028 2301 Fragments arranged by size: 2301 1028 788 761 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4161 4246 5317 4161 Size: 85 1071 4944 Fragments arranged by size: 4944 1071 85 BsmI GAATG_Cn' Cuts at: 2401 2639 3611 3704 2401 Size: 238 972 93 4797 Fragments arranged by size: 4797 972 238 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3065 3419 4161 4246 5317 1391 Size: 399 1275 354 742 85 1071 2174 Fragments arranged by size: 2174 1275 1071 742 399 354 85 BspEI T'CCGG_A Cuts at: 5763 5763 Size: 6100 BspHI T'CATG_A Cuts at: 3989 5719 3989 Size: 1730 4370 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2200 2518 3061 3455 844 Size: 1356 318 543 394 3489 Fragments arranged by size: 3489 1356 543 394 318 BsrBI CCG'CTC Cuts at: 1394 1823 3987 1394 Size: 429 2164 3507 Fragments arranged by size: 3507 2164 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4595 4769 65 Size: 1183 3347 174 1396 Fragments arranged by size: 3347 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4744 4931 1700 Size: 110 2934 187 2869 Fragments arranged by size: 2934 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6100 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6100 BssSI C'ACGA_G Cuts at: 3159 3302 4160 5172 3159 Size: 143 858 1012 4087 Fragments arranged by size: 4087 1012 858 143 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6100 BstBI TT'CG_AA Cuts at: 4904 4904 Size: 6100 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6100 BstYI r'GATC_y Cuts at: 1972 3430 3797 4182 4199 5651 5737 5749 6095 Size: 1458 367 385 17 1452 86 12 346 Cuts at: 6095 1972 Size: 1977 Fragments arranged by size: 1977 1458 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2111 513 Size: 552 939 107 4502 Fragments arranged by size: 4502 939 552 107 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5361 Fragments arranged by size: 5361 683 56 BtsI GCAGTG_nn' Cuts at: 3063 3616 4410 4430 3063 Size: 553 794 20 4733 Fragments arranged by size: 4733 794 553 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6100 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6065 DraI TTT'AAA Cuts at: 3404 3404 Size: 6100 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5107 809 Size: 507 3791 1802 Fragments arranged by size: 3791 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3547 4436 6065 Size: 54 1425 50 275 18 1717 889 1629 Cuts at: 6065 8 Size: 43 Fragments arranged by size: 1717 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3547 1537 Size: 275 1735 4090 Fragments arranged by size: 4090 1735 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4030 1353 Size: 2677 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4687 5061 5207 387 Size: 1064 138 3098 374 146 1280 Fragments arranged by size: 3098 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2127 2582 3360 4163 4760 5547 5815 Size: 393 495 455 778 803 597 787 268 Cuts at: 5815 1239 Size: 1524 Fragments arranged by size: 1524 803 787 778 597 495 455 393 268 EcoO109I rG'GnC_Cy Cuts at: 2530 3118 2530 Size: 588 5512 EcoRI G'AATT_C Cuts at: 3409 3787 3409 Size: 378 5722 FspI TGC'GCA Cuts at: 4606 4606 Size: 6100 HaeII r_GCGC'y Cuts at: 1678 2050 2227 5247 1678 Size: 372 177 3020 2531 Fragments arranged by size: 3020 2531 372 177 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2034 2060 2066 2092 2108 Size: 32 135 32 462 26 6 26 16 Cuts at: 2108 2140 2234 2266 2550 2582 3251 3283 4741 Size: 32 94 32 284 32 669 32 1458 Cuts at: 4741 4773 4815 4847 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1458 669 462 284 135 94 42 32 32 32 32 32 32 32 32 26 26 16 6 HincII GTy'rAC Cuts at: 669 1657 1938 2410 3325 3444 3690 669 Size: 988 281 472 915 119 246 3079 Fragments arranged by size: 3079 988 915 472 281 246 119 HindIII A'AGCT_T Cuts at: 748 748 Size: 6100 HpaI GTT'AAC Cuts at: 3690 3690 Size: 6100 KasI G'GCGC_C Cuts at: 2223 2223 Size: 6100 KpnI G_GTAC'C Cuts at: 3425 3425 Size: 6100 MfeI C'AATT_G Cuts at: 3699 3699 Size: 6100 MluI A'CGCG_T Cuts at: 5976 5976 Size: 6100 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5214 5398 1305 Size: 3909 184 2007 Fragments arranged by size: 3909 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4278 Fragments arranged by size: 4278 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4058 4417 4576 518 Size: 648 2892 359 159 2042 Fragments arranged by size: 2892 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3516 4193 5341 5586 1606 Size: 1910 677 1148 245 2120 Fragments arranged by size: 2120 1910 1148 677 245 NarI GG'CG_CC Cuts at: 2224 2224 Size: 6100 NcoI C'CATG_G Cuts at: 513 1065 2004 2111 513 Size: 552 939 107 4502 Fragments arranged by size: 4502 939 552 107 NdeI CA'TA_TG Cuts at: 387 2632 3025 387 Size: 2245 393 3462 Fragments arranged by size: 3462 2245 393 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6100 NotI GC'GGCC_GC Cuts at: 3547 3547 Size: 6100 NsiI A_TGCA'T Cuts at: 3254 3254 Size: 6100 NspI r_CATG'y Cuts at: 1132 1229 2607 3256 3458 5003 1132 Size: 97 1378 649 202 1545 2229 Fragments arranged by size: 2229 1545 1378 649 202 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6100 PciI A'CATG_T Cuts at: 4999 4999 Size: 6100 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6100 PmeI GTTT'AAAC Cuts at: 3404 3404 Size: 6100 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4169 4201 5682 5714 4169 Size: 32 1481 32 4555 Fragments arranged by size: 4555 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2530 2530 Size: 6100 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6100 PsiI TTA'TAA Cuts at: 3670 3670 Size: 6100 PspOMI G'GGCC_C Cuts at: 3061 3061 Size: 6100 PspXI vC'TCGA_Gb Cuts at: 2328 2328 Size: 6100 PstI C_TGCA'G Cuts at: 830 3058 3452 830 Size: 2228 394 3478 Fragments arranged by size: 3478 2228 394 PvuI CG_AT'CG Cuts at: 2001 2762 2001 Size: 761 5339 SalI G'TCGA_C Cuts at: 1936 3442 1936 Size: 1506 4594 SbfI CC_TGCA'GG Cuts at: 3452 3452 Size: 6100 ScaI AGT'ACT Cuts at: 4348 6060 4348 Size: 1712 4388 SfcI C'TryA_G Cuts at: 826 1045 2575 3054 3448 4583 5264 5455 826 Size: 219 1530 479 394 1135 681 191 1471 Fragments arranged by size: 1530 1471 1135 681 479 394 219 191 SfoI GGC'GCC Cuts at: 2225 2225 Size: 6100 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6100 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6100 SmaI CCC'GGG Cuts at: 1872 3427 1872 Size: 1555 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2328 3335 4204 5105 5367 820 Size: 197 926 385 1007 869 901 262 1553 Fragments arranged by size: 1553 1007 926 901 869 385 262 197 SnaBI TAC'GTA Cuts at: 493 3891 493 Size: 3398 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6100 SphI G_CATG'C Cuts at: 1132 3458 1132 Size: 2326 3774 SspI AAT'ATT Cuts at: 5 52 4024 5 Size: 47 3972 2081 Fragments arranged by size: 3972 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6100 StyI C'CwwG_G Cuts at: 513 1065 2004 2111 3539 513 Size: 552 939 107 1428 3074 Fragments arranged by size: 3074 1428 939 552 107 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4127 4144 4297 4482 4927 325 Size: 1255 2547 17 153 185 445 1498 Fragments arranged by size: 2547 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2519 4346 6058 Size: 274 80 33 51 1443 542 1827 1712 Cuts at: 6058 96 Size: 138 Fragments arranged by size: 1827 1712 1443 542 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2514 2529 2546 2561 6060 6092 2514 Size: 15 17 15 3499 32 2522 Fragments arranged by size: 3499 2522 32 17 15 15 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6100 XbaI T'CTAG_A Cuts at: 3436 3436 Size: 6100 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2109 2109 Size: 6100 XhoI C'TCGA_G Cuts at: 1943 2328 1943 Size: 385 5715 XmaI C'CCGG_G Cuts at: 1870 3425 1870 Size: 1555 4545 XmnI GAAnn'nnTTC Cuts at: 2808 3189 4229 2808 Size: 381 1040 4679 Fragments arranged by size: 4679 1040 381 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3908 276 Size: 53 83 186 936 2374 2468 Fragments arranged by size: 2468 2374 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI PacI PfoI PmlI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI